Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,001,918	0	A	C	20.6%	180.5 / 11.2	68	intergenic (‑129/+192)	fliA/fliC	RNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein
Reads supporting (aligned to +/- strand):  ref base A (53/1);  new base C (0/14);  total (53/15)
Fisher's exact test for biased strand distribution p-value = 1.22e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCA  >  NC_000913/2001779‑2002055                                                                                                                                             |                                                                                                                                          agTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGaaaaaaa                                                                                                                                           >  2:1506805/1‑140 (MQ=255)   tGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAAcc                                                                                                                                         >  1:1589110/1‑140 (MQ=255)    gAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAAccc                                                                                                                                        >  1:226584/1‑140 (MQ=255)      aTTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCccg                                                                                                                                      >  2:1843742/1‑140 (MQ=255)      aTTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCccg                                                                                                                                      >  1:1594839/1‑140 (MQ=255)      aTTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGAGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCccg                                                                                                                                      >  2:1878374/1‑140 (MQ=255)      aTTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGCTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCccg                                                                                                                                      >  2:1596486/1‑140 (MQ=255)                        tGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCAc                                                                                                                    >  2:404981/1‑140 (MQ=255)                        tGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCAc                                                                                                                    >  1:1081801/1‑140 (MQ=255)                          cGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGt                                                                                                                  >  1:1429924/1‑140 (MQ=255)                               tatGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTg                                                                                                             >  2:212794/1‑140 (MQ=255)                                atGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGa                                                                                                            >  2:1762315/1‑140 (MQ=255)                                    gTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACaaa                                                                                                        >  1:620867/1‑140 (MQ=255)                                    gTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACaaa                                                                                                        >  1:529694/1‑140 (MQ=255)                                    gTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACaaa                                                                                                        >  2:217290/1‑140 (MQ=255)                                    gTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACaaa                                                                                                        >  1:454111/1‑140 (MQ=255)                                     ttATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATAGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAAt                                                                                                       >  1:514903/1‑140 (MQ=255)                                       aTCGGCATGTTTATCCGTTTCTGCAGGGTTTTTAAGGGGACGATTAGGGGGTGAAATGAGGGTTTTTTGGGGGGTTA‑CAGTTAAATCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTg                                                                                                     <  2:763919/140‑1 (MQ=255)                                       aTCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTg                                                                                                     >  2:1792985/1‑140 (MQ=255)                                       aTCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTg                                                                                                     >  2:855369/1‑140 (MQ=255)                                       aTCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTg                                                                                                     >  1:253385/1‑140 (MQ=255)                                       aTCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTg                                                                                                     >  1:1218339/1‑140 (MQ=255)                                         cGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTgcg                                                                                                   >  1:1177643/1‑140 (MQ=255)                                         cGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTgcg                                                                                                   >  2:134267/1‑140 (MQ=255)                                         cGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTgcg                                                                                                   >  1:608378/1‑140 (MQ=255)                                         cGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTgcg                                                                                                   >  1:1656528/1‑140 (MQ=255)                                             aTGATTATCCTTTTCTGCGGGTTTTTTAATGGGACGATTAGGGGGTGAAATGAGGGTTTTTTTGGGGTTTA‑CAGGAAAATTCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCttt                                                                                               <  2:1594839/140‑1 (MQ=255)                                             aTGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCttt                                                                                               >  1:7021/1‑140 (MQ=255)                                              tGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGGTTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTa                                                                                              >  1:376737/1‑140 (MQ=255)                                                  tATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGtt                                                                                          >  1:255590/1‑140 (MQ=255)                                                         ttCTGCAGGTTTTTAAATCGGACGATTAGGGGGTAAAATGGGGGTTTATTGGGGGGTTA‑CAGGTAAATTCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGAt                                                                                   <  1:404981/140‑1 (MQ=255)                                                         ttCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGAt                                                                                   >  1:1141984/1‑140 (MQ=255)                                                          tCTGCAGGGTTTTTAATGGGATGATTAGTGGGTAAAAGGAGGGTTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAAAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATg                                                                                  <  2:1018479/140‑1 (MQ=255)                                                          tCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATg                                                                                  >  1:662533/1‑140 (MQ=255)                                                           cTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgc                                                                                 >  2:5345/1‑140 (MQ=255)                                                           cTGCAGGGTTTTAAATGGGACGATTAGTGGGTGAAATGAGGTGTTATTTGGGGGTTA‑CATGTAAATACCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgc                                                                                 <  2:1448292/140‑1 (MQ=255)                                                                gggTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGt                                                                            >  1:1377416/1‑140 (MQ=255)                                                                gggTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGt                                                                            >  2:736225/1‑140 (MQ=255)                                                                  ttttttAATCGGACGATTGGTGGGTGAAATGGGGGTTTATTGGGGGGTAA‑CAGGTAAATCCCAGGCAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTaa                                                                          <  2:662533/139‑1 (MQ=255)                                                                           cGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTAt                                                                 >  1:437193/1‑140 (MQ=255)                                                                            ggACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATc                                                                >  1:234168/1‑140 (MQ=255)                                                                             gACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAACTTGGGCTTTATGTTGCCGCATGCGGCGTAAACGCCTTATcc                                                               >  1:1263255/1‑140 (MQ=255)                                                                             gACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATcc                                                               >  1:393261/1‑140 (MQ=255)                                                                             gACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATcc                                                               >  1:1483574/1‑140 (MQ=255)                                                                             gACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATcc                                                               >  2:1841044/1‑140 (MQ=255)                                                                              cagATTGGGGGGTGAAAGGAGGGGTT‑TTTGGGGGTTAGGAGATAACTGCCGGGCAAAAAAACCCCCCGCCGGTGGCGGGGAAGCACGTTGCTGGCAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCg                                                              <  2:376737/138‑1 (MQ=25)                                                                                         gtaaaaTGGGGGTTTTTTTGGGGGTTA‑CAGGTAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaaa                                                   <  1:1843742/137‑1 (MQ=38)                                                                                         gtaaaaTGGGGGGTTATTGGGGGTTTA‑CAGGTAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaaa                                                   <  2:426609/137‑1 (MQ=37)                                                                                            aaaTGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtgt                                                >  2:163769/1‑140 (MQ=255)                                                                                            aaaTGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtgt                                                >  1:1808943/1‑140 (MQ=255)                                                                                              aTGGGGGTTTATTGGGGGTTTA‑CAGGTAAATCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGc                                              <  1:1762315/140‑1 (MQ=37)                                                                                              aTGAGGGGTTATTGGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGc                                              <  1:223036/140‑1 (MQ=255)                                                                                                 aGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACCCCTTATCCGGCCTACAAAAATGTGCaaa                                           >  2:1296690/1‑140 (MQ=255)                                                                                                  ggggTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAAt                                          >  1:1204424/1‑140 (MQ=255)                                                                                                      ttATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCaa                                      >  2:660394/1‑140 (MQ=255)                                                                                                        aTTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATa                                    >  2:678323/1‑140 (MQ=255)                                                                                                        aTTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATa                                    >  2:708107/1‑140 (MQ=255)                                                                                                             ggggTTA‑CAGGTAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTg                               <  1:286910/140‑1 (MQ=255)                                                                                                              gggTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGc                              >  1:1862463/1‑140 (MQ=255)                                                                                                               ggTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCa                             >  1:1476652/1‑140 (MQ=255)                                                                                                               ggTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCa                             >  1:1018228/1‑140 (MQ=255)                                                                                                                gTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCaa                            >  1:1386897/1‑140 (MQ=255)                                                                                                                   taaaGGTAAATTCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAtt                          <  2:1018228/137‑1 (MQ=255)                                                                                                                   a‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTc                         >  1:104457/1‑140 (MQ=255)                                                                                                                   a‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTc                         >  1:936510/1‑140 (MQ=255)                                                                                                                   a‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTc                         >  1:266643/1‑140 (MQ=255)                                                                                                                   a‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTc                         >  1:1096689/1‑140 (MQ=255)                                                                                                                          aaaTCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTg                   <  2:1081801/140‑1 (MQ=255)                                                                                                                          aaaTCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTg                   <  2:1386897/140‑1 (MQ=255)                                                                                                                               ccAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGc              <  2:936510/140‑1 (MQ=255)                                                                                                                                       aaaaaacCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAag      <  1:1878374/140‑1 (MQ=255)                                                                                                                                       aaaaaaaCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAag      >  2:578126/1‑140 (MQ=255)                                                                                                                                       aaaaaaaCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAag      <  1:1296690/140‑1 (MQ=255)                                                                                                                                           aaaCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGCTGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgca  <  2:1141984/140‑1 (MQ=255)                                                                                                                                           aaaCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgca  <  2:7021/140‑1 (MQ=255)                                                                                                                                             |                                                                                                                                          AGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTA‑CAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCA  >  NC_000913/2001779‑2002055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑