Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,740,909	0	T	G	24.7%	165.9 / 15.1	73	V402G (GTG→GGG)	avtA	valine‑‑pyruvate aminotransferase
Reads supporting (aligned to +/- strand):  ref base T (13/42);  new base G (18/0);  total (31/42)
Fisher's exact test for biased strand distribution p-value = 3.75e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGT  >  NC_000913/3740770‑3741045                                                                                                                                            |                                                                                                                                         tATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGt                                                                                                                                          <  1:547342/140‑1 (MQ=255)        gcCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGaat                                                                                                                                   >  2:723748/1‑138 (MQ=255)        gcCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGAtt                                                                                                                                   >  2:1052947/1‑140 (MQ=255)         ccTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTc                                                                                                                                  >  1:1766739/1‑140 (MQ=255)           tGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTg                                                                                                                                <  1:832735/140‑1 (MQ=255)           tGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTg                                                                                                                                <  1:1596212/140‑1 (MQ=255)             aaaGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGc                                                                                                                              >  2:1872439/1‑140 (MQ=255)             aaaGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGc                                                                                                                              <  1:204301/140‑1 (MQ=255)             aaaGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGc                                                                                                                              <  1:1417576/140‑1 (MQ=255)                gCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGa                                                                                                                           <  2:1446945/140‑1 (MQ=255)                 cACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGGTTCTGGCGGaa                                                                                                                          <  1:861113/140‑1 (MQ=255)                 cACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGaa                                                                                                                          <  1:1052947/140‑1 (MQ=255)                    gcggcgTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAagag                                                                                                                       <  2:704030/140‑1 (MQ=255)                    gcggcgTGCTGATGGTGCCGGGGCACAACTTCTT‑CCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAgaga                                                                                                                      <  2:569459/140‑1 (MQ=255)                          tGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGaa                                                                                                                 <  2:784874/140‑1 (MQ=255)                                tGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCGATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGcc                                                                                                           <  2:1015474/140‑1 (MQ=255)                                     ccGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGGAGAGATAGAAAGAGCCTgggg                                                                                                      >  2:155921/1‑139 (MQ=255)                                         ggCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGaa                                                                                                  >  1:1724755/1‑140 (MQ=255)                                            acaACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGATGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGt                                                                                               >  1:403197/1‑140 (MQ=255)                                               aCTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCAc                                                                                            <  2:1014454/140‑1 (MQ=255)                                               aCTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCAc                                                                                            <  2:1931374/140‑1 (MQ=255)                                                   cttcCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGGCTGGGCTGAAAGTCACTaaa                                                                                        >  2:1161305/1‑140 (MQ=255)                                                   cttcCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAg                                                                                                <  2:70480/132‑1 (MQ=255)                                                   cttcCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAg                                                                                                >  1:70480/1‑132 (MQ=255)                                                     tcCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTaaaaa                                                                                      <  2:443166/140‑1 (MQ=255)                                                         aGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGc                                                                                  <  2:1385312/140‑1 (MQ=255)                                                         aGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGc                                                                                  <  1:155921/140‑1 (MQ=255)                                                           ggCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGc                                                                                  <  2:1276944/138‑1 (MQ=255)                                                           ggCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGc                                                                                  >  1:1276944/1‑138 (MQ=255)                                                             cTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGAGTCTGGCGGAAAAGATAGAAAGAGCCTGGGCTGAAAGGCACTAAAAAATGCCTGa                                                                              >  2:1167687/1‑140 (MQ=255)                                                             cTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGGAGAGGTAAAAAGAGGCTGGGGTGAAAGGCAATAAAAATTGccctt                                                                              >  2:1262647/1‑137 (MQ=255)                                                                gATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATgc                                                                           >  1:1074818/1‑140 (MQ=255)                                                                gATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGCAGGTTCTGGCGGGAGAGATAGAAAGAGCCTGGGCTGTAAGGGACTAAAAAATGTCTGATgc                                                                           >  2:1376498/1‑140 (MQ=255)                                                                gATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATgc                                                                           >  2:194227/1‑140 (MQ=255)                                                                 aTAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATgcg                                                                          <  1:1425569/140‑1 (MQ=255)                                                                 aTAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATgcg                                                                          <  1:1022507/140‑1 (MQ=255)                                                                      cggTGGCCGCATACGCATCAATTTATGGGCAGGAACTGCGTACAAGGGCCGAAGAAAATTGGGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGGGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTAct                                                                     <  2:249918/138‑2 (MQ=255)                                                                      ccGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAGGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACg                                                                     <  2:1786447/140‑1 (MQ=255)                                                                      ccGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACg                                                                     <  2:1186417/140‑1 (MQ=255)                                                                      ccGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACg                                                                     <  1:1405489/140‑1 (MQ=255)                                                                      ccGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACg                                                                     <  1:1603849/140‑1 (MQ=255)                                                                      ccGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACg                                                                     >  1:424067/1‑140 (MQ=255)                                                                      ccGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACg                                                                     >  1:1763805/1‑140 (MQ=255)                                                                          ggCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTa                                                                 >  1:930135/1‑140 (MQ=255)                                                                           gCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTAt                                                                <  1:391280/140‑1 (MQ=255)                                                                           gCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTAt                                                                >  1:881177/1‑140 (MQ=255)                                                                           gCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGACTGATGCGCTACGCTTAt                                                                >  2:999866/1‑140 (MQ=255)                                                                                aTACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGc                                                           <  1:681541/140‑1 (MQ=255)                                                                                    gCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTAc                                                       <  1:1751357/140‑1 (MQ=255)                                                                                    gCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTAc                                                       <  1:194227/140‑1 (MQ=255)                                                                                         aaTGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGAt                                                  <  2:424067/140‑1 (MQ=255)                                                                                         aaTGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGAt                                                  <  1:1376498/140‑1 (MQ=255)                                                                                         aaTGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGAt                                                  <  2:1763805/140‑1 (MQ=255)                                                                                               tGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGc                                            <  2:980580/140‑1 (MQ=255)                                                                                                gcgcATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCa                                           <  1:82535/140‑1 (MQ=255)                                                                                                gcgcATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCa                                           <  1:1872439/140‑1 (MQ=255)                                                                                                gcgcATGAACCACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCa                                           <  2:484592/140‑1 (MQ=255)                                                                                                  gcATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCa                                           <  1:1849/138‑1 (MQ=255)                                                                                                  gcATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCa                                           >  2:1849/1‑138 (MQ=255)                                                                                                   cATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAata                                        >  1:1643406/1‑140 (MQ=255)                                                                                                   cATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAata                                        >  1:1949016/1‑140 (MQ=255)                                                                                                   cATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAata                                        >  2:374053/1‑140 (MQ=255)                                                                                                     tGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAatata                                      >  2:1384985/1‑140 (MQ=255)                                                                                                       aaCTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATAtt                                    <  1:1535702/140‑1 (MQ=255)                                                                                                            cGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCCACATGATTTCTGGAATATATTTAAtt                               >  1:1276815/1‑140 (MQ=255)                                                                                                            cGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTAGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAAtt                               >  2:1247293/1‑140 (MQ=255)                                                                                                            cGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTAGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAAtt                               >  2:19775/1‑140 (MQ=255)                                                                                                              tACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTg                             <  1:1721200/140‑1 (MQ=255)                                                                                                              tACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTg                             >  2:392519/1‑140 (MQ=255)                                                                                                              tACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGCCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTg                             >  1:1416708/1‑140 (MQ=255)                                                                                                                ccAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTCGCGGAAGAGACAGAAAGAGCCATGGCTGAAAGTCACTAAAAAATGCCTGATGCGCTACCCTTAACCAGCCTACATGATTTCTGCAATATATTGAATTTTga                           >  2:1115964/1‑140 (MQ=37)                                                                                                                 cAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCa                                           <  2:263556/123‑1 (MQ=255)                                                                                                                 cAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCa                                           >  1:263556/1‑123 (MQ=255)                                                                                                                 cAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGgaa                          >  2:1842615/1‑140 (MQ=255)                                                                                                                  agagCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGgaag                         <  1:1262647/140‑1 (MQ=255)                                                                                                                  agagCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGgaag                         <  1:1167687/140‑1 (MQ=255)                                                                                                                   gagCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAAAGCCTGGGCTGAAAGTCACTAAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTTgaaga                        >  2:227044/1‑140 (MQ=255)                                                                                                                      ccGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAAtt                     >  1:450050/1‑140 (MQ=255)                                                                                                                         gagaAAATTGAGGCGGGGGGGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGt                  >  1:535775/1‑140 (MQ=255)                                                                                                                           gaAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAg                <  2:1724755/140‑1 (MQ=255)                                                                                                                                         gggTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCACGCCTACATGATTTCTGCAATATATTTAATTTGGAAGAATTTGTAGGCCGGATaaagagc  >  2:360440/1‑135 (MQ=255)                                                                                                                                            |                                                                                                                                         TATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGT  >  NC_000913/3740770‑3741045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑