Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,494,080	G→T	A537A (GCG→GCT)	trg →	methyl‑accepting chemotaxis protein Trg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,494,080	0	G	T	100.0%	286.1 / NA	84	A537A (GCG→GCT)	trg	methyl‑accepting chemotaxis protein Trg
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (47/37);  total (47/37)

CGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATC  >  NC_000913/1493944‑1494213                                                                                                                                         |                                                                                                                                      cGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGt                                                                                                                                    >  1:13172/1‑140 (MQ=255) cGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGt                                                                                                                                    >  1:1861709/1‑140 (MQ=255) cGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGt                                                                                                                                    >  2:724891/1‑140 (MQ=255) cGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGt                                                                                                                                    >  1:28297/1‑140 (MQ=255) cGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGt                                                                                                                                    >  1:867192/1‑140 (MQ=255) cGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGAATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGt                                                                                                                                    >  1:1938902/1‑140 (MQ=255)        gAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCag                                                                                                                             <  2:1781563/140‑1 (MQ=255)         aaTGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCagt                                                                                                                            <  2:676555/140‑1 (MQ=255)         aaTGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCagt                                                                                                                            <  2:28297/140‑1 (MQ=255)            gCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCagttag                                                                                                                         <  2:867192/140‑1 (MQ=255)            gCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCagttag                                                                                                                         <  1:1932334/140‑1 (MQ=255)             cTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAAACTCGCGGAGCTGGTGAACCagttagt                                                                                                                        >  2:628263/1‑140 (MQ=255)                ctctGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTc                                                                                                                     >  1:1517312/1‑140 (MQ=255)                   tGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCagttag                                                                                                                         <  2:323354/133‑1 (MQ=255)                   tGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCagttag                                                                                                                         >  1:323354/1‑133 (MQ=255)                   tGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCt                                                                                                                  >  1:924734/1‑140 (MQ=255)                    ggTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCAGGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCCCGCGGAGCTGGTGAACCAGTTAGTTTCGCTa                                                                                                                 <  2:42018/140‑1 (MQ=255)                    ggTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTa                                                                                                                 <  2:1732424/140‑1 (MQ=255)                    ggTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTa                                                                                                                 <  2:1861709/140‑1 (MQ=255)                    ggTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTa                                                                                                                 <  1:1264187/140‑1 (MQ=255)                       agaagaGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTa                                                                                                                 >  2:689945/1‑137 (MQ=255)                       agaagaGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTa                                                                                                                 <  1:689945/137‑1 (MQ=255)                        gaagaGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACggg                                                                                                             >  1:1010587/1‑139 (MQ=255)                         aagaGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGgtg                                                                                                            <  1:1674854/140‑1 (MQ=255)                          agagGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGgtgg                                                                                                           >  1:1521655/1‑139 (MQ=255)                           gagGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGtgtg                                                                                                          >  2:543408/1‑140 (MQ=255)                             ggCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTGACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGaa                                                                                                        <  1:490264/140‑1 (MQ=255)                             ggCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCCGTTAGTTTCGCTACGGTGTGaa                                                                                                        >  1:1235733/1‑140 (MQ=255)                             ggCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGGGAACCAGTTAGTTTCGCTACGGTGGGaa                                                                                                        >  1:1845930/1‑140 (MQ=255)                              gCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACg                                                                                                               <  1:1263124/132‑1 (MQ=255)                              gCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACg                                                                                                               >  2:1263124/1‑132 (MQ=255)                               ccTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGGACCCGGTAGTTTCGCTTCGGTGTGaaaa                                                                                                      >  2:432062/1‑140 (MQ=255)                               ccTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGaaaa                                                                                                      >  1:1043192/1‑140 (MQ=255)                               ccTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGaaa                                                                                                       >  2:1872565/1‑139 (MQ=255)                               ccTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGaaa                                                                                                       <  1:1872565/139‑1 (MQ=255)                                cTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAAt                                                                                                     >  2:1518496/1‑140 (MQ=255)                                cTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAAt                                                                                                     >  1:1171965/1‑140 (MQ=255)                                cTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGGGAACCAGTTAGTTTCGCTACGGTGTGAAAAt                                                                                                     >  1:1755747/1‑140 (MQ=255)                                       gcggcggTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAag                                                                                              <  2:420562/140‑1 (MQ=255)                                       gcggcggTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAag                                                                                              <  1:724891/140‑1 (MQ=255)                                       gcggcggTGCCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAag                                                                                              <  2:1818777/140‑1 (MQ=255)                                         ggcggTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAagga                                                                                            <  2:1951470/140‑1 (MQ=255)                                              tgtCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGAt                                                                                       <  1:1518496/140‑1 (MQ=255)                                                   cTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACa                                                                                  <  2:1531453/140‑1 (MQ=255)                                                   cTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACa                                                                                  <  2:1517312/140‑1 (MQ=255)                                                   cTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACa                                                                                  <  1:597678/140‑1 (MQ=255)                                                   cTTGAAGAACAGGCAGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTGTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGATACCGTGTGAAAATGTTCAAGGAGGGATCGACa                                                                                  <  2:405873/140‑1 (MQ=255)                                                          aaCAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCt                                                                           >  1:1554551/1‑140 (MQ=255)                                                          aaCAGGCGGCACGATTAACTGAGGCGGTGAATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACTAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCt                                                                           >  2:1383006/1‑140 (MQ=255)                                                             aGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCa                                                                        <  2:1521589/140‑1 (MQ=255)                                                              ggcggcACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGGGAACCCGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGGTCGACAGATCCCTTCAc                                                                       >  2:369407/1‑140 (MQ=255)                                                               gcggcACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCTGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCAcc                                                                      >  1:999322/1‑140 (MQ=255)                                                               gcggcACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCAcc                                                                      >  1:1774910/1‑140 (MQ=255)                                                                 ggcACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCtt                                                                          <  2:765217/134‑1 (MQ=255)                                                                 ggcACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCtt                                                                          >  1:765217/1‑134 (MQ=255)                                                                 ggcACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCtt                                                                    >  2:403886/1‑140 (MQ=255)                                                                 ggcACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCtt                                                                    >  1:380167/1‑140 (MQ=255)                                                                 ggcACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCtt                                                                    >  2:270256/1‑140 (MQ=255)                                                                     cGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAg                                                                <  1:403886/140‑1 (MQ=255)                                                                            cTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCa                                                         <  2:1043192/140‑1 (MQ=255)                                                                            cTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCa                                                         <  1:628263/140‑1 (MQ=255)                                                                                ggCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGa                                                     >  2:659833/1‑140 (MQ=255)                                                                                 gCGGTGGATGTATTCCGTCTGCACAAACATTCTTTTTCGGCAGAACCTCGCGGGGCTGGGGAACCCGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGAt                                                    >  2:1365942/1‑140 (MQ=255)                                                                                 gCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTTAGAACGGCATTGAt                                                    >  1:1276923/1‑140 (MQ=255)                                                                                  cGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGAtt                                                   >  1:1891046/1‑140 (MQ=255)                                                                                           tATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAg                                          >  2:1633659/1‑140 (MQ=255)                                                                                             ttCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCg                                        <  2:13172/140‑1 (MQ=255)                                                                                              tCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGt                                       <  2:1774910/140‑1 (MQ=255)                                                                                              tCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGt                                       <  2:608693/140‑1 (MQ=255)                                                                                              tCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGt                                       <  2:573326/140‑1 (MQ=255)                                                                                                 gTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTaa                                    >  1:192003/1‑140 (MQ=255)                                                                                                  tCTGCACAAACATTCTGTGTCGGCAGAACCTGGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAat                                   >  2:1643387/1‑140 (MQ=255)                                                                                                  tCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAat                                   >  2:1340171/1‑140 (MQ=255)                                                                                                  tCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAat                                   >  2:1259687/1‑140 (MQ=255)                                                                                                      cacaAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAatcatc                               >  2:749630/1‑140 (MQ=255)                                                                                                      cacaAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAatcatc                               <  2:1938902/140‑1 (MQ=255)                                                                                                      cacaAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAatcatc                               >  1:690214/1‑140 (MQ=255)                                                                                                      cacaAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAatcatc                               <  2:1598293/140‑1 (MQ=255)                                                                                                              ttCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCa                       >  1:995791/1‑140 (MQ=255)                                                                                                              ttCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCa                       <  2:1171965/140‑1 (MQ=255)                                                                                                              ttCTGTGGCGGCAGAACCGCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGACCCCTTCACCTTTCAGAGCGGCATTGATTTTCGAATAGCGTTACTCCACAACTggaa                       <  2:1719983/140‑3 (MQ=255)                                                                                                                cTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAAc                     >  1:1243841/1‑140 (MQ=255)                                                                                                                  gtgtCGGCAGAACCTCGCGGAGCTGGTGAACTAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCa                   <  1:1383006/140‑1 (MQ=255)                                                                                                                             aCCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGt        >  2:993727/1‑140 (MQ=255)                                                                                                                             aCCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGt        <  2:454821/140‑1 (MQ=255)                                                                                                                             aCCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGt        >  2:1885753/1‑140 (MQ=255)                                                                                                                             aCCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGt        >  2:305719/1‑140 (MQ=255)                                                                                                                             aCCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGt        >  2:337127/1‑140 (MQ=255)                                                                                                                               cTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCg      >  2:1688613/1‑140 (MQ=255)                                                                                                                                 cgcgGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCa    >  1:1934954/1‑140 (MQ=255)                                                                                                                                 cgcgGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCa    >  2:369145/1‑140 (MQ=255)                                                                                                                                   cgGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATc  >  1:1144926/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      CGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATC  >  NC_000913/1493944‑1494213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑