Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913650,8090TG27.3% 226.2 / 32.9 110T350P (ACC→CCC) citCcitrate lyase synthetase
Reads supporting (aligned to +/- strand):  ref base T (28/52);  new base G (30/0);  total (58/52)
Fisher's exact test for biased strand distribution p-value = 5.95e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 123 total aligned reads displayed.

CTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGATAT  >  NC_000913/650670‑650947
                                                                                                                                           |                                                                                                                                          
ctcAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGt                                                                                                                                            <  2:846753/140‑1 (MQ=255)
   aaCGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTTACCTGTTTCTCATGCGGGGGGCtt                                                                                                                                         >  1:665217/1‑140 (MQ=255)
     cGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCtttt                                                                                                                                       >  1:1418254/1‑140 (MQ=255)
     cGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCtttt                                                                                                                                       >  2:1889170/1‑140 (MQ=255)
      gctgctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTg                                                                                                                                      <  1:609062/140‑1 (MQ=255)
       ctgctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCCTTTTTCACCTGTTTCTCATGCGGGGGGCTTTTgg                                                                                                                                     >  2:1162850/1‑139 (MQ=255)
        tgctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGAc                                                                                                                                    >  1:1996000/1‑140 (MQ=255)
          ctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGa                                                                                                                                  >  2:180384/1‑140 (MQ=255)
          ctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTCATTTTTTTCCTGTTTTTCATGCGGGGGGCTTTTGACGa                                                                                                                                  >  2:1412599/1‑140 (MQ=255)
                  ttGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCcgc                                                                                                                          <  1:222700/140‑1 (MQ=255)
                  ttGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCcgc                                                                                                                          <  1:296015/140‑1 (MQ=255)
                    gCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGt                                                                                                                        <  1:1055277/140‑1 (MQ=255)
                    gCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGt                                                                                                                        >  1:96339/1‑140 (MQ=255)
                     cAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTc                                                                                                                       >  1:1858688/1‑140 (MQ=255)
                       ggTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACTAGCTGCCGCGTCCt                                                                                                                     >  1:1169722/1‑140 (MQ=255)
                          cGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGc                                                                                                                  >  2:106144/1‑140 (MQ=255)
                             tatCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCggg                                                                                                               <  1:329891/140‑1 (MQ=255)
                             tatCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCggg                                                                                                               <  1:1412599/140‑1 (MQ=255)
                                ccTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGt                                                                                                            <  2:1697871/140‑1 (MQ=255)
                                 cTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTg                                                                                                           <  2:2190410/140‑1 (MQ=255)
                                  tGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTTTTTTCATTTTTTACCTGTTTCTCATGCGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGCgggggggg                                                                                                          >  2:969030/1‑135 (MQ=255)
                                  tGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTTTTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCCGGCgggggggg                                                                                                          >  2:592794/1‑135 (MQ=255)
                                  tGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGCGGGAgggg                                                                                                          >  1:2235220/1‑137 (MQ=255)
                                  tGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGGGc                                                                                                          >  1:730332/1‑140 (MQ=255)
                                  tGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGGGCCTGCAACGGCGGGCTGGGTTATTTTCTTTTTTCACCTTGTTCTCCTACGGGGGGGTTTTGACCAGCTGGCGCGCGCTGGGGGGAGTGc                                                                                                          >  2:937293/1‑140 (MQ=255)
                                   gCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCt                                                                                                         <  1:1889170/140‑1 (MQ=255)
                                    cGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCCGGTTTATTTTCATTTTTTACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGGCCTGGGGGGGGTgacc                                                                                                        >  2:1060375/1‑137 (MQ=255)
                                       aTCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTTTTTTCATTTTTTACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGGTCaaa                                                                                                     >  2:743289/1‑139 (MQ=255)
                                       aTCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTA‑TTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGTGCTCaaac                                                                                                    >  1:1780814/1‑138 (MQ=255)
                                        tCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGc                                                                                                    <  1:2135053/140‑1 (MQ=255)
                                        tCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGc                                                                                                    <  2:352469/140‑1 (MQ=255)
                                         cGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGc                                                                                                    <  1:987809/139‑1 (MQ=255)
                                         cGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCa                                                                                                   >  1:240057/1‑140 (MQ=255)
                                           aGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAgg                                                                                                 >  1:79897/1‑140 (MQ=255)
                                            gTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGt                                                                                                <  1:1300144/140‑1 (MQ=255)
                                            gTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTTATTTTTCACCCGTTTCTCATACGGGGGGTTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGcgagt                                                                                                >  2:411261/1‑136 (MQ=255)
                                              ggggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTTTTTTTATTTTTCACCTTTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAGCAgggtg                                                                                              >  2:1600539/1‑137 (MQ=255)
                                              ggggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGGGGTCAAGCAAGttt                                                                                              >  1:1529177/1‑139 (MQ=255)
                                               gggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCt                                                                                             <  1:180384/140‑1 (MQ=255)
                                               gggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCt                                                                                             <  1:22060/140‑1 (MQ=255)
                                               gggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCt                                                                                             <  1:2138145/140‑1 (MQ=255)
                                               gggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCt                                                                                             <  2:930235/140‑1 (MQ=255)
                                               gggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCt                                                                                             <  1:1162850/140‑1 (MQ=255)
                                               gggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCt                                                                                             <  2:1996000/140‑1 (MQ=255)
                                               gggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCCGGTTTATTTTTATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACCAGCTGCCGCGTCCTGGCGGGGGGGGCCAAGCCGGTTCt                                                                                             >  1:1823459/1‑140 (MQ=255)
                                                     tGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAaat                                                                                       <  1:1437701/140‑1 (MQ=255)
                                                       cGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAaataa                                                                                     <  1:592794/140‑1 (MQ=255)
                                                            catcaCATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAg                                                                                <  2:1867054/140‑1 (MQ=255)
                                                               cacaTCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTTTTTTCATTTTTTACCTGTTTTTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCCGGCGGGGGGGGTCCAACAGGTTTTTCAAAAAAATCAGCgg                                                                             >  2:226235/1‑139 (MQ=255)
                                                               cacaTCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGt                                                                             <  1:506519/140‑1 (MQ=255)
                                                               cacaTCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGt                                                                             <  1:106144/140‑1 (MQ=255)
                                                                acaTCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTg                                                                            <  2:1858688/140‑1 (MQ=255)
                                                                acaTCCCCAGACTCAAGAGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTg                                                                            <  2:596850/140‑1 (MQ=255)
                                                                 caTCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTTTTTTCATTTTTTACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGGTCAAACAGGGTTTGCAAAAAATGCAGCgggg                                                                           >  2:609179/1‑137 (MQ=255)
                                                                 caTCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTTACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGGGCTCAAGCAGGTTCTTCAAAAAATGGAGCGTGa                                                                           >  1:2222293/1‑140 (MQ=255)
                                                                 caTCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAGCAGGGTCTGCAAATAATGCAGCGTGa                                                                           >  1:1379735/1‑140 (MQ=255)
                                                                   tCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTCCTTTTTCCCCTGTTTTTCCTGCGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGCGGGGGTGCTCCAGCAGGGTCTTCAAAAAATGCGACGGGACt                                                                         >  2:1468320/1‑140 (MQ=255)
                                                                   tCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTCATTTTTCACCTGTTTCTCTTGCGGGGGGCTTTTGACCGGCCGCCCCGTCCTGGCGGGGGTGCTCCAGCAGGTGTTGCCAAAAATGGAGCGGGagg                                                                         >  2:586725/1‑138 (MQ=255)
                                                                    ccccAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCCCCCTGTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGGGGGAGGGGTCAAACAGGTTTTTCAAAAAAAGCAGCGTGGCtt                                                                        >  2:1965553/1‑139 (MQ=255)
                                                                    ccccAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTg                                                                        >  1:164106/1‑140 (MQ=255)
                                                                    ccccAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTg                                                                        <  1:1925493/140‑1 (MQ=255)
                                                                    ccccAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAGCAGGGTTTTCCAAAAATGCAGCGGGGCTg                                                                        >  2:1925493/1‑140 (MQ=255)
                                                                      ccAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTTTTTTCATTTTTCACCCGTTTCTCATGCGGGGGGCTTTTTTCGAGCCGCCGCGTCCTGGCGGGGGGGCTTACGGAGGGTCTGCAAATAATGGAGCGTGGCGGCa                                                                      >  2:1622606/1‑140 (MQ=255)
                                                                      ccAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTTACCTGTTTCTCATGCGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAACAGGGTTTTCAAATAATGCAGCGGGACTGCa                                                                      >  2:2145122/1‑140 (MQ=255)
                                                                      ccAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCa                                                                      <  2:240057/140‑1 (MQ=255)
                                                                          aCTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGGTCTGCAAAAAATGCAGCGTGGCTGCCgggg                                                                  >  1:24255/1‑139 (MQ=255)
                                                                              aaGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAg                                                              >  1:2089627/1‑140 (MQ=255)
                                                                              aaGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAACAGGTTCTGCCAAAAATGCAGCGTGACTGCAGGGAcccg                                                              >  1:861127/1‑138 (MQ=255)
                                                                                 ggTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAgcgg                                                           <  1:1057871/140‑1 (MQ=255)
                                                                                   tGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAgcggcg                                                         <  2:37082/140‑1 (MQ=255)
                                                                                     ccTGCAACGGCGGGCTGGTTTTTTTTTCTTTTTTACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCAGCCGCGTCCTCGCGGGGGTGCTCCAAGAGGGTTTGGAAAAAATGGAAGGTGATTGCAGGGGGCAGGGGggcg                                                       >  2:1127056/1‑140 (MQ=255)
                                                                                     ccTGCAACGGCGGGCTGGTTTATTTTCCTTTTTTACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAACAGGGTCTGCAAATAAAGCAGCGTGGCTGCAGGGGCCACCGGcgcg                                                       >  2:626506/1‑140 (MQ=255)
                                                                                     ccTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGcgcg                                                       >  1:1968828/1‑140 (MQ=255)
                                                                                     ccTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGcgcg                                                       >  1:1475331/1‑140 (MQ=255)
                                                                                     ccTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGGGCTCAAGCAGGGTCTGCAAATAATGCAGCGGGACTGCAGGGACCAGCGGcgcg                                                       >  1:1090928/1‑140 (MQ=255)
                                                                                      cTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGa                                                      <  1:969030/140‑1 (MQ=255)
                                                                                      cTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGa                                                      <  1:1651879/140‑1 (MQ=255)
                                                                                             ggcggGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGt                                               <  1:1600539/140‑1 (MQ=255)
                                                                                                  gCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACTAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGAt                                          <  2:1169722/140‑1 (MQ=255)
                                                                                                         tATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGttttt                                   >  1:46456/1‑140 (MQ=255)
                                                                                                               cATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCcag                             <  1:744998/140‑1 (MQ=255)
                                                                                                                aTTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGGGATCGTTTTTCGCcagc                            >  2:333524/1‑140 (MQ=255)
                                                                                                                aTTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGc                                <  2:1383996/136‑1 (MQ=255)
                                                                                                                   tttCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGt                         <  1:1127056/140‑1 (MQ=255)
                                                                                                                      cACCTGTTTCTTATGCGGGGGTTTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGa                      <  1:2247101/140‑1 (MQ=255)
                                                                                                                      cACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGa                      <  1:2047846/140‑1 (MQ=255)
                                                                                                                        ccTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACg                    <  1:573754/140‑1 (MQ=255)
                                                                                                                          tGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTa                  >  1:488854/1‑140 (MQ=255)
                                                                                                                           gTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACATAc                 >  2:1367509/1‑140 (MQ=255)
                                                                                                                            tttCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTAcc                >  2:709752/1‑140 (MQ=255)
                                                                                                                            tttCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTAcc                >  2:1110005/1‑140 (MQ=255)
                                                                                                                              tctcATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCg              >  2:1537064/1‑140 (MQ=255)
                                                                                                                               ctcATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCgg             >  2:2079607/1‑140 (MQ=255)
                                                                                                                                tcATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGGCCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCggg            <  1:1965553/140‑1 (MQ=255)
                                                                                                                                   tGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACg                    >  1:2268668/1‑129 (MQ=255)
                                                                                                                                   tGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACg                    <  2:2268668/129‑1 (MQ=255)
                                                                                                                                    gCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGc        >  1:98999/1‑140 (MQ=255)
                                                                                                                                      gggggTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCgg      <  1:559841/140‑1 (MQ=255)
                                                                                                                                      gggggTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCgg      >  2:600909/1‑140 (MQ=255)
                                                                                                                                      gggggTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCgg      >  1:1465593/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
CTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGATAT  >  NC_000913/650670‑650947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑