Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913753,1250TA24.0% 230.3 / 30.1 100intergenic (‑330/+60)ybgD/gltAputative fimbrial protein YbgD/citrate synthase
Reads supporting (aligned to +/- strand):  ref base T (65/11);  new base A (0/24);  total (65/35)
Fisher's exact test for biased strand distribution p-value = 5.23e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 116 total aligned reads displayed.

GGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGT  >  NC_000913/752986‑753264
                                                                                                                                            |                                                                                                                                            
gggTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCatat                                                                                                                                              <  2:156466/140‑1 (MQ=255)
gggTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCatat                                                                                                                                              <  2:1921239/140‑1 (MQ=255)
     ataAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATaaaaa                                                                                                                                         >  1:373230/1‑140 (MQ=255)
     ataAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATaaaaa                                                                                                                                         >  2:582259/1‑140 (MQ=255)
     ataAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATaaaaa                                                                                                                                         >  2:2158986/1‑140 (MQ=255)
      taAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAAt                                                                                                                                        >  1:1721626/1‑140 (MQ=255)
           gCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑Acc                                                                                                                                  >  2:993345/1‑140 (MQ=255)
           gCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑Acc                                                                                                                                  >  2:1612076/1‑140 (MQ=255)
            cGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑Accc                                                                                                                                 >  2:248458/1‑140 (MQ=255)
            cGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑Accc                                                                                                                                 >  2:2221675/1‑140 (MQ=255)
              aCTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGc                                                                                                                               >  1:522837/1‑140 (MQ=255)
                 cccATGAATTTTTTAATTGAAAGTATTGGGTTGTTGATATTTTGAGCTTTTTTTTTTTTTTTAAATATTTATATAGGTTTGTTAATGGTTTTTTTTTTTACATTTTTTTGTGTTT‑AGGCATAAAAAAACCA‑CCCCGCCat                                                                                                                            <  2:537496/139‑1 (MQ=255)
                 aCCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCat                                                                                                                            >  2:900087/1‑140 (MQ=255)
                    aTGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATg                                                                                                                         >  2:1613915/1‑140 (MQ=255)
                         ttttttAATTGAAAGTTTTGGTTTGCTGATAATTTGAGCTTTTTTATTTTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTAAAATTTTTTGTTTTT‑AGGCATAAAAAAATCA‑CCCCGCCATATGAAcgg                                                                                                                    <  2:1806109/139‑1 (MQ=255)
                         ttttttAATTGAAAGTATTGGGTTGTTGATAATTTGAGTTTTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGTTTTTATTTTTAAAATTTTTTGTGTTT‑AGGCATATAAAAACCA‑ACCCGCCATATGAAcgg                                                                                                                    <  2:1937395/139‑1 (MQ=255)
                         ttttttAATTAAAATTTTTGGGTTGTTAATATTTTGAGCTTTTCTTTTTTTTTAAAATATCTATATAGGTCTGTTAATGGTTTTTATTTTTACATTTTTTTGTTTTT‑AGGCATAAAAAAATCA‑ACCCGCCATATGAAcgg                                                                                                                    <  2:1842402/139‑1 (MQ=255)
                         ttttttAATTAAAAGTATTGGTTTGCTGATATTTTGAGCTTTTTTTTTTTTTTTAAATTTCTATATAGGTTTGTTAATGGATTTTTTTTTTAAATTTTTTTGTTTTT‑AGGCATAAAAAAATCA‑ACCCGCCATATGAAcgg                                                                                                                    <  2:1721626/139‑1 (MQ=255)
                            tttAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAAcggcgg                                                                                                                 >  1:1842402/1‑140 (MQ=255)
                            tttAATTGAAAGTATTGGGTTGCTGATAATTTGACCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAAcggcgg                                                                                                                 >  1:501569/1‑140 (MQ=255)
                            tttAATTGAAAGCATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAAcggcgg                                                                                                                 >  1:2002574/1‑140 (MQ=255)
                                  taaaaGTATTGGTTTGTTTATAATTTGGGCTTTTCTTTTTTTTTAAAATATTTATATAGGTCTGTAAATGGTTTTTTTTTTTACATTTTTTTGTTTTT‑AGCCTTAAAAAAATCA‑CCCCGCCATATGAACGGCGGGTTaaa                                                                                                           <  2:2017955/138‑1 (MQ=255)
                                  tGAAAGTTTTGGTTTGCTGATATTTTGAGCTTTTCTTTTTTTTTTAAATATCTATATAGGTCTTTTAATGGTTTTTTTTTTTACAATTTTTTGTTTTT‑AGAAATAAAAAAACCA‑ACCCGCCATATGAACGGCGGGTTaaa                                                                                                           <  2:522837/140‑1 (MQ=255)
                                    aaaGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTaaaaa                                                                                                         >  2:1068307/1‑139 (MQ=255)
                                    aaaGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAat                                                                                                         >  1:1570671/1‑140 (MQ=255)
                                    aaaGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAat                                                                                                         >  1:1495650/1‑140 (MQ=255)
                                    aaaGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAat                                                                                                         >  2:1555484/1‑140 (MQ=255)
                                    aaaGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGAATTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTaaaaa                                                                                                         >  2:388055/1‑139 (MQ=255)
                                          ttGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATAttttc                                                                                                   >  2:63935/1‑138 (MQ=255)
                                          ttGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTAc                                                                                                   >  1:1407069/1‑140 (MQ=255)
                                               ttgttaatatTTTGAGTTTTTTTTTTTTTTTTAAATTTTTATATAGGTTTTTTAATGTTTTTTTTTTTTACATTTTTTTGTTTTT‑AGGAATAAAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACtt                                                                                              <  2:700282/134‑1 (MQ=255)
                                                  cTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGc                                                                                           >  1:2043480/1‑140 (MQ=255)
                                                  cTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGc                                                                                           >  1:1444735/1‑140 (MQ=255)
                                                  cTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGc                                                                                           >  2:1288237/1‑140 (MQ=255)
                                                  cTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGc                                                                                           >  1:816615/1‑140 (MQ=255)
                                                  cTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGc                                                                                           >  1:700282/1‑140 (MQ=255)
                                                     aTAATTTGAGTTGTTTTTTTTTTTTAAAATTTTTATATTTTTCTGTTAATGGTTTTTATTTTTACATTTTTTTGTTTTT‑AGGAATATAAAATTCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAAt                                                                                        <  2:1075602/140‑1 (MQ=255)
                                                       aaTTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCa                                                                                      >  1:1088204/1‑140 (MQ=255)
                                                         tttGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTAAAACTTAGCAATCAAc                                                                                    >  1:684833/1‑140 (MQ=255)
                                                            gAGCTTTTCTATTTTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTTTTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAACCA‑CCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCAt                                                                                 <  1:388055/140‑1 (MQ=255)
                                                              ttttttttATTTTTTTTAAATATTTATATAGGTTTTTTAATGGTTTTTTTTTTTACATTTTTTTGTTTTT‑AGGAATAAAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTa                                                                               <  2:1351272/133‑1 (MQ=255)
                                                                 gTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACg                                                                            >  2:2038970/1‑140 (MQ=255)
                                                                  ttCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGc                                                                           >  1:79654/1‑140 (MQ=255)
                                                                   tCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCCATCAACCATTAAAGCt                                                                          >  1:2222511/1‑140 (MQ=255)
                                                                         ttttttAAATATCTATATATGTCTTTTAATGTATTTTTTTTTTAAATTTTTTTGTTTTT‑AGGAATAAAAAAACCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGatat                                                                    <  2:2182216/139‑1 (MQ=255)
                                                                         ttttttAAATATCTATATAGGTCTGTTAATGGTTTTTATTTTTACAATTTTTTGTTTTT‑AGGCATAAAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGatat                                                                    <  1:1223440/139‑1 (MQ=255)
                                                                          tttttAAATATCTATATAGGTTTGTTAATGGATTTTTTTTTTACATTTTTTTGTTTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATc                                                                   <  1:993345/140‑1 (MQ=255)
                                                                            tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTTAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGc                                                                 >  2:1464941/1‑140 (MQ=255)
                                                                            tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATctg                                                                 >  1:1776251/1‑138 (MQ=255)
                                                                            tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGc                                                                 >  1:296472/1‑140 (MQ=255)
                                                                            tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGc                                                                 >  2:1588823/1‑140 (MQ=255)
                                                                            tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGc                                                                 >  1:456624/1‑140 (MQ=255)
                                                                               aaaTATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCttt                                                              >  1:1363202/1‑140 (MQ=255)
                                                                                 atatCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTa                                                            <  1:2221675/140‑1 (MQ=255)
                                                                                      tatataGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTc                                                       >  2:1435193/1‑140 (MQ=255)
                                                                                          tgggTCTGTTAATGATTTTTTTTTTTACATTTTTTTGTTTTT‑AGGCATAAAAAAACCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGt                                                   <  2:22433/138‑1 (MQ=255)
                                                                                          taGGTTTTTTAATGTTTTTTTTTTTTAAATTTTTTTGTTTTT‑AGGAATAAAAAAATCA‑CCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGt                                                   <  2:501569/140‑1 (MQ=255)
                                                                                          taGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGt                                                   <  1:1588823/140‑1 (MQ=255)
                                                                                             gTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGtttt                                                >  2:2007678/1‑140 (MQ=255)
                                                                                             gTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGtttt                                                >  1:2009378/1‑140 (MQ=255)
                                                                                             gTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGtttt                                                >  1:2231443/1‑140 (MQ=255)
                                                                                               cTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTc                                              >  1:2243533/1‑140 (MQ=255)
                                                                                                tGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCa                                             >  1:1266080/1‑140 (MQ=255)
                                                                                                tGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCa                                             >  1:460194/1‑140 (MQ=255)
                                                                                                tGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCa                                             >  1:1907934/1‑140 (MQ=255)
                                                                                                tGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCa                                             >  1:2182093/1‑140 (MQ=255)
                                                                                                tGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCa                                             >  2:1012936/1‑140 (MQ=255)
                                                                                                tGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCa                                             >  2:1535199/1‑140 (MQ=255)
                                                                                                tGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCa                                             >  2:1158934/1‑140 (MQ=255)
                                                                                                 tttAATGTATTTTATTTTTACATTTTTTTGTTTTT‑AGGAAAAAAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                                            <  2:1195526/139‑1 (MQ=255)
                                                                                                 tttAATGGTTTTTATTTTTACAATTTTTTGTTTTT‑AGACATAAAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                                            <  2:1444735/139‑1 (MQ=255)
                                                                                                 tttAATGGATTTTTTTTTTAAAATTTTTTGTTTTT‑AGGCATAAAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                                            <  2:891833/139‑1 (MQ=255)
                                                                                                 gtaaaTGGA‑TTTATTTTT‑TAATTTTTTTTGTTTAAAGTTTATAAAAAAAATGCCCGCCATATGAACGGTGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTTat                                            <  2:184144/137‑1 (MQ=255)
                                                                                                  ttAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGt                                                   <  1:980175/132‑1 (MQ=255)
                                                                                                  ttAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGt                                                   >  2:980175/1‑132 (MQ=255)
                                                                                                  ttAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCata                                           >  2:711440/1‑140 (MQ=255)
                                                                                                    aatgttttttattttTAAATTTTTTTGTTTTT‑AGAAATAAAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATc                                         <  2:2231443/134‑1 (MQ=255)
                                                                                                    aatgatttttttttttAAATTTTTTTGTTTTT‑AGGAATAAAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATc                                         <  2:203245/134‑1 (MQ=255)
                                                                                                    aaTGGTTTTTTTTTTTAAATTTTTTTGTTTTT‑AGGAATAAAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATc                                         <  2:1864881/140‑1 (MQ=255)
                                                                                                    aaTGGATTTTATTTTTACAATTTTTTGTTTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATc                                         <  1:996967/140‑1 (MQ=255)
                                                                                                    aaTGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATc                                         >  1:1072930/1‑140 (MQ=255)
                                                                                                    aaTGGATTTTATTTTTAAAATTTTTTTTTTTT‑GGCCATAAAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATc                                         <  1:633357/140‑1 (MQ=255)
                                                                                                       ggATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTg                                      >  1:1904593/1‑140 (MQ=255)
                                                                                                            ttattttTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATAc                                 >  2:2153591/1‑140 (MQ=255)
                                                                                                            ttattttTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATAc                                 >  1:922008/1‑140 (MQ=255)
                                                                                                                  ttACATTTTTTTGTTTTT‑AGGAATAAAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGa                           <  2:1266080/140‑1 (MQ=255)
                                                                                                                  ttACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGa                           >  1:1417654/1‑140 (MQ=255)
                                                                                                                    aCAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGAcg                         <  1:1288237/140‑1 (MQ=255)
                                                                                                                     cAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTTAAGTCGCGTTTTTCATATCCTGTATACAGCTGAcgc                        >  1:159821/1‑140 (MQ=255)
                                                                                                                      atttttttttGTTT‑AGGAAAAAAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGAcgcg                       <  2:456624/138‑1 (MQ=255)
                                                                                                                        ttttttGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGa                     >  1:663782/1‑140 (MQ=255)
                                                                                                                         tttttGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGAc                    <  1:1535199/140‑1 (MQ=255)
                                                                                                                             tgtgTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGc                <  2:1088204/140‑1 (MQ=255)
                                                                                                                             tgtgTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGc                <  1:1012936/140‑1 (MQ=255)
                                                                                                                                   t‑AGGCATAAAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCtt          <  1:1487652/140‑1 (MQ=255)
                                                                                                                                    tagaaataaAAAAACCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCtt          <  2:1379910/135‑1 (MQ=255)
                                                                                                                                      ggCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCa        <  1:2035023/140‑1 (MQ=255)
                                                                                                                                         ataaAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATAc     <  2:1393255/140‑1 (MQ=255)
                                                                                                                                            taAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTTACGCTTGATTTCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGt  >  1:2030677/1‑140 (MQ=255)
                                                                                                                                            taAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGt  >  2:124000/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                            
GGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTT‑AGGCATATAAAAATCA‑ACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGT  >  NC_000913/752986‑753264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑