BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,216,538	C→A	S354* (TCG→TAG)	aceB →	malate synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,216,538	0	C	A	99.2%	416.9 / ‑3.1	117	S354* (TCG→TAG)	aceB	malate synthase A
Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (67/49); total (68/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

Only 100 of 117 total aligned reads displayed.

GAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGC  >  NC_000913/4216401‑4216670

gAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGc                                                                                                                                    >  1:81113/1‑140 (MQ=255)

  aTGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTg                                                                                                                                  <  2:1148763/140‑1 (MQ=255)

   tGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTgg                                                                                                                                 >  1:847568/1‑140 (MQ=255)

   tGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTgg                                                                                                                                 <  1:331870/140‑1 (MQ=255)

   tGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTgg                                                                                                                                 >  1:1452084/1‑140 (MQ=255)

     cTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGaa                                                                                                                               <  2:2029955/140‑1 (MQ=255)

      ttACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAg                                                                                                                              >  1:157827/1‑140 (MQ=255)

        aCTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGcc                                                                                                                            <  1:83919/140‑1 (MQ=255)

        aCTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGcc                                                                                                                            >  2:1015573/1‑140 (MQ=255)

        aCTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGcc                                                                                                                            >  2:2232219/1‑140 (MQ=255)

        aCTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGcc                                                                                                                            >  2:2142707/1‑140 (MQ=255)

        aCTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGcc                                                                                                                            >  2:1197756/1‑140 (MQ=255)

             cGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATaa                                                                                                                       >  2:1123710/1‑140 (MQ=255)

               ccTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTg                                                                                                                                  >  1:110419/1‑127 (MQ=255)

               ccTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTg                                                                                                                                  <  2:110419/127‑1 (MQ=255)

                cTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACgg                                                                                                                    >  2:1011509/1‑140 (MQ=255)

                 tGTAGATAAAACCCTGCCAGAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTGTAGTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGt                                                                                                                   <  2:1102166/140‑1 (MQ=255)

                   ttGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCa                                                                                                                 <  1:1588453/140‑1 (MQ=255)

                   ttGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCa                                                                                                                 <  2:1345076/140‑1 (MQ=255)

                   ttGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCa                                                                                                                 >  2:1279307/1‑140 (MQ=255)

                   ttGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCa                                                                                                                 >  1:2185777/1‑140 (MQ=255)

                    tGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCAc                                                                                                                >  2:926236/1‑140 (MQ=255)

                    tGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCAc                                                                                                                >  2:1120874/1‑140 (MQ=255)

                     gATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACg                                                                                                               <  1:525608/140‑1 (MQ=255)

                     gATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACg                                                                                                               <  1:683394/140‑1 (MQ=255)

                      aTTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGa                                                                                                              <  2:682642/140‑1 (MQ=255)

                      aTTAAAACCTGCCATAAACGCGGTGCTTTTGCGACGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGa                                                                                                              <  1:1963542/140‑1 (MQ=255)

                       ttAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGAt                                                                                                             <  1:1508018/140‑1 (MQ=255)

                       ttAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGAt                                                                                                             <  1:1689983/140‑1 (MQ=255)

                       ttAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGAt                                                                                                             <  2:505413/140‑1 (MQ=255)

                       ttAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGAt                                                                                                             <  1:1123710/140‑1 (MQ=255)

                        tAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATg                                                                                                            >  1:1311125/1‑140 (MQ=255)

                        tAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACg                                                                                                               >  2:426169/1‑137 (MQ=255)

                        tAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACg                                                                                                               <  1:426169/137‑1 (MQ=255)

                          aaaCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTGAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGc                                                                                                          >  2:496465/1‑140 (MQ=255)

                             ccTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGcaca                                                                                                       >  1:1431860/1‑140 (MQ=255)

                                gCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGAt                                                                                                             >  2:111097/1‑131 (MQ=255)

                                gCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGAt                                                                                                             <  1:111097/131‑1 (MQ=255)

                                gCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATgg                                                                                                    >  1:1137227/1‑140 (MQ=255)

                                gCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATgg                                                                                                    >  2:761349/1‑140 (MQ=255)

                                gCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATgg                                                                                                    >  2:1682203/1‑140 (MQ=255)

                                 ccATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGAt                                                                                                             >  2:458460/1‑130 (MQ=255)

                                 ccATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGAt                                                                                                             <  1:458460/130‑1 (MQ=255)

                                 ccATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGa                                                                                                   >  2:523440/1‑140 (MQ=255)

                                    tAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCg                                                                                                <  1:1015573/140‑1 (MQ=255)

                                     aaaCGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGc                                                                                               <  2:2068795/140‑1 (MQ=255)

                                          cgGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCAcc                                                                                          <  1:1471128/140‑1 (MQ=255)

                                          cgGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCAcc                                                                                          <  1:489821/140‑1 (MQ=255)

                                            gTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCa                                                                                        <  1:2255832/140‑1 (MQ=255)

                                                       aTGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGa                                                                             >  2:2053946/1‑140 (MQ=255)

                                                       aTGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGa                                                                             >  2:813186/1‑140 (MQ=255)

                                                       aTGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGa                                                                             >  2:1753640/1‑140 (MQ=255)

                                                       aTGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGa                                                                             >  2:234329/1‑140 (MQ=255)

                                                       aTGGGCGGCATGGCGGCGTTTATTCCGAACAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGa                                                                             >  2:767932/1‑140 (MQ=255)

                                                         gggCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGaca                                                                           >  1:852041/1‑140 (MQ=255)

                                                            cggcATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGATCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACgg                                                                        >  2:1634673/1‑140 (MQ=255)

                                                             ggcATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGc                                                                       <  1:1999887/140‑1 (MQ=255)

                                                                  ggcggcGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATgg                                                                  >  2:242726/1‑140 (MQ=255)

                                                                   gcggcgTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGc                                                                 <  1:520555/140‑1 (MQ=255)

                                                                    cggcgTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGTAATGGCg                                                                >  1:319810/1‑140 (MQ=255)

                                                                    cggcgTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATgg                                                                  >  1:555739/1‑138 (MQ=255)

                                                                     ggcgTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCgg                                                               <  1:2053946/140‑1 (MQ=255)

                                                                              tCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACg                                                      <  2:352598/140‑1 (MQ=255)

                                                                                  aGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACAt                                                  >  2:139014/1‑140 (MQ=255)

                                                                                       aGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCg                                             >  2:178193/1‑140 (MQ=255)

                                                                                       aGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCg                                             >  2:915559/1‑140 (MQ=255)

                                                                                           gAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTc                                         <  1:1995012/140‑1 (MQ=255)

                                                                                           gAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTc                                         <  2:81113/140‑1 (MQ=255)

                                                                                           gAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTc                                         <  1:1430494/140‑1 (MQ=255)

                                                                                           gAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTc                                         <  1:234329/140‑1 (MQ=255)

                                                                                           gAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTc                                         <  1:813186/140‑1 (MQ=255)

                                                                                                  acaATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTaaa                                  >  2:1928348/1‑140 (MQ=255)

                                                                                                  acaATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTaaa                                  >  2:742905/1‑140 (MQ=255)

                                                                                                    aaTAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTaaaaa                                >  2:1128659/1‑140 (MQ=255)

                                                                                                           aGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCtt                         >  2:681805/1‑140 (MQ=255)

                                                                                                           aGGTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCtt                         >  2:2025109/1‑140 (MQ=255)

                                                                                                            ggTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAAt                               >  1:1104922/1‑133 (MQ=255)

                                                                                                            ggTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTg                        <  1:1727194/140‑1 (MQ=255)

                                                                                                            ggTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTg                        >  2:254404/1‑140 (MQ=255)

                                                                                                             gTGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGa                       >  1:265094/1‑140 (MQ=255)

                                                                                                              tGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCGCATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAAACAGCTTGaa                      >  2:1731508/1‑140 (MQ=255)

                                                                                                              tGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACTGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGaa                      >  1:466495/1‑140 (MQ=255)

                                                                                                              tGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGaa                      >  1:739549/1‑140 (MQ=255)

                                                                                                              tGCTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGaa                      >  2:1407935/1‑140 (MQ=255)

                                                                                                                cTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGGTCCCGTAAAAATCAGCTTGAAGt                    >  2:1127616/1‑140 (MQ=255)

                                                                                                                cTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGt                    >  2:943741/1‑140 (MQ=255)

                                                                                                                cTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGt                    >  2:2205079/1‑140 (MQ=255)

                                                                                                                cTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGt                    >  1:557521/1‑140 (MQ=255)

                                                                                                                cTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGt                    >  1:2192593/1‑140 (MQ=255)

                                                                                                                cTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGt                    >  1:171536/1‑140 (MQ=255)

                                                                                                                cTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGt                    >  1:144939/1‑140 (MQ=255)

                                                                                                                cTCAACAAAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGCATTCAACGAAATTCTCGGCTCCCGTAAAAATCAGCTTGAAGt                    >  2:1638051/1‑140 (MQ=255)

                                                                                                                    acaaAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATg                >  1:2188343/1‑140 (MQ=255)

                                                                                                                    acaaAGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATg                >  1:2014215/1‑140 (MQ=255)

                                                                                                                      aaaGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATgtg              <  2:1452084/140‑3 (MQ=255)

                                                                                                                       aaGTAAAAGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATgcgc             >  2:1989579/1‑140 (MQ=255)

                                                                                                                            aaaGCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACa        >  1:959746/1‑140 (MQ=255)

                                                                                                                               gCGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGa     >  1:2113947/1‑140 (MQ=255)

                                                                                                                                cGGATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTGAACGACCTTCTCGGGTCCCGGAAAAATCAGCTTGAAGTGATGCGCGAACAAGAc    >  2:1021498/1‑140 (MQ=255)

                                                                                                                                  gATAAATAGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGAcgc  >  2:649769/1‑140 (MQ=255)

GAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGC  >  NC_000913/4216401‑4216670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`