Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,956,076	G→T	H937N (CAC→AAC)	ptrA ←	protease 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,956,076	0	G	T	100.0%	293.6 / NA	84	H937N (CAC→AAC)	ptrA	protease 3
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (49/35);  total (49/35)

GGCGCTCACCCTGTAAGGGCAAGCGCAAAGGATCTAGTGTCTCGGCGACATCACTCATTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTGTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTT  >  NC_000913/2955946‑2956210                                                                                                                                   |                                                                                                                                       ggCGCTCACCCTGTAAGGGCAAGCGCAAAGGATCTAGTGTCTCGGCGACATCACTCATTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTc                                                                                                                               >  1:785857/1‑140 (MQ=255) ggCGCTCACCCTGTAAGGGCAAGCGCAAAGGATCTAGTGTCTCGGCGACATCACTCATTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTc                                                                                                                               >  1:1291570/1‑140 (MQ=255) ggCGCTCACCCTGTAAGGGCAAGCGCAAAGGATCTAGTGTCTCGGCGACATCACTCATTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTc                                                                                                                               >  2:883482/1‑140 (MQ=255) ggCGCTCACCCTGTAAGGGCAAGCGCAAAGGATCTAGTGTCTCGGCGACATCACTCATTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTc                                                                                                                               >  1:1654892/1‑140 (MQ=255)  gcgcTCACCCTGTAAGGGCAAGCGCAAAGGATCTAGTGTCTCGGCGACATCACTCATTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCg                                                                                                                              >  1:734187/1‑140 (MQ=255)      tCACCCTGTAAGGGCAAGCGCAAAGGATCTAGTGTCTCGGCGACATCACTCATTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCtt                                                                                                                          >  2:566077/1‑140 (MQ=255)      tCACCCTGTAAGGGCAAGCGCAAAGGATCTAGTGTCTCGGCGACATCACTCATTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCtt                                                                                                                          >  1:450992/1‑140 (MQ=255)        aCCCTGTAAGGGCAAGCGCAAAGGATCTAGTGTCTCGGCGACATCACTCATTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTc                                                                                                                        <  2:1654302/140‑1 (MQ=255)             gTAAGGGCAAGCGCAAAGGATCTAGTGTCTCGGCGACATCACTCATTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGtt                                                                                                                   <  1:605979/140‑1 (MQ=255)             gTAAGGGCAAGCGCAAAGGATCTAGTGTCTCGGCGACATCACTCATTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGtt                                                                                                                   >  2:655780/1‑140 (MQ=255)              tAAGGGCAAGCGCAAAGGATCTAGTGTCTCGGCGACATCACTCATTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTc                                                                                                                  >  1:1362790/1‑140 (MQ=255)               aaGGGCAAGCGCAAAGGATCTAGTGTCTCGGCGACATCACTCATTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCt                                                                                                                 >  1:784805/1‑140 (MQ=255)                  ggCAAGCGCAAAGGATCTAGTGTCTCGGCGACATCACTCATTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGc                                                                                                              >  1:322854/1‑140 (MQ=255)                    cAAGCGCAAAGGATCTAGTGTCTCGGCGACATCACTCATTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTg                                                                                                            >  1:855316/1‑140 (MQ=255)                    cAAGCGCAAAGGATCTAGTGTCTCGGCGACATCACTCATTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTg                                                                                                            >  1:694130/1‑140 (MQ=255)                        cgcAAAGGATCTAGTGTCTCGGCGACATCACTCATTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCgg                                                                                                        >  2:1272472/1‑140 (MQ=255)                        cgcAAAGGATCTAGTGTCTCGGCGACATCACTCATTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCgg                                                                                                        >  2:1123728/1‑140 (MQ=255)                            aaGGATCTAGTGTCTCGGCGACATCACTCATTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAAt                                                                                                    >  1:1166588/1‑140 (MQ=255)                                  cTAGTGTCTCGGCGACATCACTCATTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGa                                                                                              <  1:1502966/140‑1 (MQ=255)                                     gtgtCTCGGCGACATCACTCATTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAg                                                                                           <  2:322854/140‑1 (MQ=255)                                     gtgtCTCGGCGACATCACTCATTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAg                                                                                           <  2:583362/140‑1 (MQ=255)                                        tctcGGCGACATCACTCATTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAAt                                                                                        >  1:1465741/1‑140 (MQ=255)                                         ctcGGCGACATCACTCATTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATa                                                                                       >  1:895905/1‑140 (MQ=255)                                         ctcGGCGACATCACTCATTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATa                                                                                       >  2:1373063/1‑140 (MQ=255)                                            ggCGACATCACTCATTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGcc                                                                                    <  2:1130033/140‑1 (MQ=255)                                             gCGACATCACTCATTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCa                                                                                   >  2:354068/1‑140 (MQ=255)                                                   tCACTCATTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCtt                                                                             <  2:16415/140‑1 (MQ=255)                                                      cTCATTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCg                                                                          <  2:1349506/140‑1 (MQ=255)                                                         aTTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCt                                                                       <  2:734187/140‑1 (MQ=255)                                                         aTTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCt                                                                       <  2:1166588/140‑1 (MQ=255)                                                              tttCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGacc                                                                  >  2:219900/1‑140 (MQ=255)                                                              tttCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGacc                                                                  <  1:1272472/140‑1 (MQ=255)                                                               ttCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGacct                                                                 <  2:515728/140‑2 (MQ=255)                                                               ttCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGacca                                                                 >  1:1147773/1‑140 (MQ=255)                                                               ttCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGacca                                                                 >  1:1474572/1‑140 (MQ=255)                                                               ttCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGacca                                                                 >  1:1512828/1‑140 (MQ=255)                                                                    tcatcaGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGcc                                                            >  1:81099/1‑140 (MQ=255)                                                                     catcaGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCt                                                           >  2:1220161/1‑140 (MQ=255)                                                                     catcaGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCt                                                           >  1:1615036/1‑140 (MQ=255)                                                                       tcaGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGa                                                         <  2:450992/140‑1 (MQ=255)                                                                       tcaGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGa                                                         <  1:1368915/140‑1 (MQ=255)                                                                           gggCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGga                                                     >  2:393484/1‑140 (MQ=255)                                                                                ttGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGaaa                                                >  1:968282/1‑140 (MQ=255)                                                                                ttGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGaaa                                                <  2:785857/140‑1 (MQ=255)                                                                                     tgctgcAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGc                                           >  2:606621/1‑140 (MQ=255)                                                                                     tgctgcAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGc                                           >  2:61691/1‑140 (MQ=255)                                                                                     tgctgcAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGc                                           >  2:1168544/1‑140 (MQ=255)                                                                                       ctgcAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCaa                                         >  2:88088/1‑140 (MQ=255)                                                                                       ctgcAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCaa                                         >  2:143468/1‑140 (MQ=255)                                                                                       ctgcAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCaa                                         >  2:1051398/1‑140 (MQ=255)                                                                                          cAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCAAGtt                                      >  1:106906/1‑140 (MQ=255)                                                                                          cAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCAAGtt                                      >  2:1759080/1‑140 (MQ=255)                                                                                           aaCGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCAAGttt                                     >  1:34005/1‑140 (MQ=255)                                                                                             cgcgCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCAAGttttt                                   >  2:1369363/1‑140 (MQ=255)                                                                                             cgcgCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCAAGttttt                                   >  2:1410988/1‑140 (MQ=255)                                                                                             cgcgCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCAAGttttt                                   <  2:1668368/140‑1 (MQ=255)                                                                                                    aCGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGt                            <  2:1465741/140‑1 (MQ=255)                                                                                                     cGTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTc                           <  1:143468/140‑1 (MQ=255)                                                                                                      gTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTca                          <  2:1291570/140‑1 (MQ=255)                                                                                                      gTTCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTca                          <  2:784805/140‑1 (MQ=255)                                                                                                       ttCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTcag                         <  2:1147773/140‑1 (MQ=255)                                                                                                       ttCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTcag                         <  2:1654892/140‑1 (MQ=255)                                                                                                       ttCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTcag                         <  2:1474572/140‑1 (MQ=255)                                                                                                       ttCTCCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTcag                         <  1:219900/140‑1 (MQ=255)                                                                                                          tcCCACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTcagcag                      <  1:61691/140‑1 (MQ=255)                                                                                                           cccACACTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGt                     <  2:952755/140‑1 (MQ=255)                                                                                                              acacTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGtttt                  >  2:369470/1‑140 (MQ=255)                                                                                                              acacTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGtttt                  >  1:347387/1‑140 (MQ=255)                                                                                                                acTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTAt                >  2:438601/1‑140 (MQ=255)                                                                                                                acTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTAt                >  1:60093/1‑140 (MQ=255)                                                                                                                 cTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCTGCAGTTTTATc               <  2:694130/140‑1 (MQ=255)                                                                                                                 cTTTCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATc               <  1:1369363/140‑1 (MQ=255)                                                                                                                  tttCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCt              >  2:1072468/1‑140 (MQ=255)                                                                                                                    tCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTgg            >  1:995269/1‑140 (MQ=255)                                                                                                                    tCCAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTgg            >  1:465341/1‑140 (MQ=255)                                                                                                                      cAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGc          <  2:968282/140‑1 (MQ=255)                                                                                                                      cAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGc          <  1:354068/140‑1 (MQ=255)                                                                                                                      cAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGc          <  1:369470/140‑1 (MQ=255)                                                                                                                      cAGCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGc          <  1:606621/140‑1 (MQ=255)                                                                                                                        gCCTTCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCa        <  1:438601/140‑1 (MQ=255)                                                                                                                           ttCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGa     <  2:895905/140‑1 (MQ=255)                                                                                                                            tCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGc          >  1:1645529/1‑134 (MQ=255)                                                                                                                            tCAGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGc          <  2:1645529/134‑1 (MQ=255)                                                                                                                              aGGGTTTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGAttt  >  2:128056/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                       GGCGCTCACCCTGTAAGGGCAAGCGCAAAGGATCTAGTGTCTCGGCGACATCACTCATTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGGTGTACATATTCGGCTTTCCCGTTCTGGCTGCCGGAAATCTGCGACAGAATAGCCATGCCTTGCGGCTCGACCACCGCCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTT  >  NC_000913/2955946‑2956210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑