Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,995,156	C→A	A132D (GCT→GAT)	dapF →	diaminopimelate epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,995,156	0	C	A	100.0%	346.4 / NA	97	A132D (GCT→GAT)	dapF	diaminopimelate epimerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (44/53);  total (44/53)

GACTGACCAATAAGCGTGATATCCGCGTCAGCACCGCCAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGCTAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTGCGTGATTCAGGTCGATGATGTCGATACCGCGGCGGTAGAAACGCTTGGTC  >  NC_000913/3995024‑3995290                                                                                                                                     |                                                                                                                                       gACTGACCAATAAGCGTGATATCCGCGTCAGCACCGCCAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACaaa                                                                                                                                 >  2:1424255/1‑140 (MQ=255)    tGACCAATAAGCGTGATATCCGCGTCAGCACCGCCAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCg                                                                                                                              >  1:1309561/1‑140 (MQ=255)      aCCAATAAGCGTGATATCCGCGTCAGCACCGCCAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGa                                                                                                                            >  1:782250/1‑140 (MQ=255)       ccAATAAGCGTGATATCCGCGTCAGCACCGCCAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGaa                                                                                                                           <  2:1198896/140‑1 (MQ=255)       ccAATAAGCGTGATATCCGCGTCAGCACCGCCAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGaa                                                                                                                           <  1:252988/140‑1 (MQ=255)       ccAATAAGCGTGATATCCGCGTCAGCACCGCCAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGaa                                                                                                                           <  1:1569193/140‑1 (MQ=255)       ccAATAAGCGTGATATCCGCGTCAGCACCGCCAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGaa                                                                                                                           <  1:1412249/140‑1 (MQ=255)       ccAATAAGCGTGATATCCGCGTCAGCACCGCCAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGaa                                                                                                                           <  1:1305916/140‑1 (MQ=255)       ccAATAAGCGTGATATCCGCGTCAGCACCGCCAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGaa                                                                                                                           <  2:206095/140‑1 (MQ=255)       ccAATAAGCGTGATATCCGCGTCAGCACCGCCAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGaa                                                                                                                           <  1:1033334/140‑1 (MQ=255)         aaTAAGCGTGATATCCGCGTCAGCACCGCCAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGaaaa                                                                                                                         <  1:1370287/140‑1 (MQ=255)         aaTAAGCGTGATATCCGCGTCAGCACCGCCAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGaaaa                                                                                                                         <  2:697887/140‑1 (MQ=255)           tAAGCGTGATATCCGCGTCAGCACCGCCAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCTGTGCCGTTTCGCGATAACAAAGCGGAAAAGa                                                                                                                       <  1:1089011/140‑1 (MQ=255)           tAAGCGTGATATCCGCGTCAGCACCGCCAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGa                                                                                                                       >  2:931980/1‑140 (MQ=255)           tAAGCGTGATATCCGCGTCAGCACCGCCAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGa                                                                                                                       <  1:270728/140‑1 (MQ=255)            aaGCGTGATATCCGCGTCAGCACCGCCAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGAc                                                                                                                      <  1:1382538/140‑1 (MQ=255)            aaGCGTGATATCCGCGTCAGCACCGCCAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGAc                                                                                                                      <  1:308679/140‑1 (MQ=255)                gTGATATCCGCGTCAGCACCGCCAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGaaaa                                                                                                                         <  2:298369/133‑1 (MQ=255)                gTGATATCCGCGTCAGCACCGCCAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGaaaa                                                                                                                         >  1:298369/1‑133 (MQ=255)                 tGATATCCGCGTCAGCACCGCCAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCtata                                                                                                                 <  2:319347/140‑1 (MQ=255)                  gATATCCGCGTCAGCACCGCCAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTatat                                                                                                                >  2:1497795/1‑140 (MQ=255)                     atCCGCGTCAGCACCGCCAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTAtattat                                                                                                             >  1:702112/1‑140 (MQ=255)                        cgcgTCAGCACCGCCAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATgcg                                                                                                          >  1:696862/1‑140 (MQ=255)                         gcgTCAGCACCGCCAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATgcgc                                                                                                         >  1:456560/1‑140 (MQ=255)                         gcgTCAGCACCGCCAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATgcgc                                                                                                         >  1:101711/1‑140 (MQ=255)                          cgTCAGCACCGCCAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATgcgcg                                                                                                        <  1:518007/140‑1 (MQ=255)                            tCAGCACCGCCAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGcgcc                                                                                                      <  2:1361196/140‑1 (MQ=255)                            tCAGCACCGCCAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGcgcc                                                                                                      >  1:1468667/1‑140 (MQ=255)                             cAGCACCGCCAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGcgccg                                                                                                     <  1:931980/140‑1 (MQ=255)                                  ccGCCAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAg                                                                                                <  2:995087/140‑1 (MQ=255)                                  ccGCAAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAg                                                                                                <  1:1370376/140‑1 (MQ=255)                                    gCCAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCa                                                                                              >  1:660541/1‑140 (MQ=255)                                     ccAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAg                                                                                             <  1:1735880/140‑1 (MQ=255)                                      cAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGa                                                                                            <  1:1099681/140‑1 (MQ=255)                                      cAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGa                                                                                            <  2:473849/140‑1 (MQ=255)                                      cAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGa                                                                                            <  2:630043/140‑1 (MQ=255)                                      cAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGa                                                                                            <  1:564950/140‑1 (MQ=255)                                        aCGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACa                                                                                          <  1:987459/140‑1 (MQ=255)                                           ggCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATc                                                                                       <  2:1468667/140‑1 (MQ=255)                                             cGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCtt                                                                                     <  2:660541/140‑1 (MQ=255)                                                aTGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATg                                                                                  <  1:1718185/140‑1 (MQ=255)                                                    ttCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATgcggc                                                                              <  1:388454/140‑1 (MQ=255)                                                     tCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATgcggcg                                                                             <  2:696862/140‑1 (MQ=255)                                                      cTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCgt                                                                            >  1:1752804/1‑140 (MQ=255)                                                       tGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCgtg                                                                           <  1:1163023/140‑1 (MQ=255)                                                            gTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTc                                                                      <  2:1525545/140‑1 (MQ=255)                                                            gTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTc                                                                      >  1:793297/1‑140 (MQ=255)                                                             tCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCg                                                                     <  1:25472/140‑1 (MQ=255)                                                              cACCGATGATGATCTGGTGCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATCCGGCGTGGTGTCGa                                                                    >  2:550413/1‑140 (MQ=255)                                                              cACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGa                                                                    >  2:258470/1‑140 (MQ=255)                                                              cACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGa                                                                    >  1:901167/1‑140 (MQ=255)                                                              cACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGa                                                                    >  2:674839/1‑140 (MQ=255)                                                              cACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGa                                                                    >  1:392505/1‑140 (MQ=255)                                                               aCCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGAt                                                                   <  1:3215/140‑1 (MQ=255)                                                                 cGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATgg                                                                 <  1:423990/140‑1 (MQ=255)                                                                   atgatgatCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGa                                                               <  1:1424255/140‑1 (MQ=255)                                                                        gatCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATcc                                                          >  1:1793033/1‑140 (MQ=255)                                                                         atCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCg                                                         >  2:451821/1‑140 (MQ=255)                                                                           cTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCa                                                       <  2:702112/140‑1 (MQ=255)                                                                             ggTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCAGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCa                                                       >  2:879163/1‑138 (MQ=255)                                                                             ggTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCAGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCa                                                       <  1:879163/138‑1 (MQ=255)                                                                              gTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGAt                                                                   >  2:93748/1‑125 (MQ=255)                                                                              gTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGAt                                                                   <  1:93748/125‑1 (MQ=255)                                                                              gTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTg                                                    >  1:981747/1‑140 (MQ=255)                                                                               tCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTGc                                                   >  1:1689442/1‑140 (MQ=255)                                                                                ccGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTGCg                                                  <  2:1309561/140‑1 (MQ=255)                                                                                 cgcgTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTGCGt                                                 >  2:539772/1‑140 (MQ=255)                                                                                   cgTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTGCGTGa                                               >  2:1597704/1‑140 (MQ=255)                                                                                   cgTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTGCGTGa                                               >  2:730567/1‑140 (MQ=255)                                                                                     tAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTGCGTGAtt                                             <  1:1645293/140‑1 (MQ=255)                                                                                     tAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTGCGTGAtt                                             <  2:1078830/140‑1 (MQ=255)                                                                                     tAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTGCGTGAtt                                             <  1:1494227/140‑1 (MQ=255)                                                                                     tAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAAACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTGCGTGAtt                                             <  1:1723424/140‑1 (MQ=255)                                                                                        atatGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTGCGTGATTCAg                                          <  1:1018541/140‑1 (MQ=255)                                                                                             ggCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTGCGTGATTCAGGTCga                                     >  1:1295909/1‑140 (MQ=255)                                                                                              gCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTGCGTGATTCAGGTCgat                                    <  2:1467316/140‑1 (MQ=255)                                                                                              gCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTGCGTGATTCAGGTCgat                                    <  2:1793033/140‑1 (MQ=255)                                                                                                 aaCCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTGCGTGATTCAGGTCgatgat                                 >  1:1089701/1‑140 (MQ=255)                                                                                                  aCCCAACTTCGAACCTTCCGCCGTGTCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTGCGTGATTCAGGTCGatgatg                                >  1:1687494/1‑140 (MQ=255)                                                                                                  aCCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTGCGTGATTCAGGTCGatgatg                                >  2:1604604/1‑140 (MQ=255)                                                                                                   cccAACTTCGAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTGCGTGATTCAGGTCGATGATGt                               >  1:1421877/1‑140 (MQ=255)                                                                                                            gAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTGCGTGATTCAGGTCGATGATGTCGATACcgc                      >  2:229667/1‑140 (MQ=255)                                                                                                            gAACCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTGCGTGATTCAGGTCGATGATGTCGATACcgc                      >  1:980354/1‑140 (MQ=255)                                                                                                              aCCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTGCGTGATTCAGGTCGATGATGTCGATACCgcgg                    >  2:1274696/1‑140 (MQ=255)                                                                                                              aCCTTCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTGCGTGATTCAGGTCGATGATGTCGATACCgcgg                    <  1:727370/140‑1 (MQ=255)                                                                                                                  tCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTGCGTGATTCAGGTCGATGATGTCGATACCGCGGCGGt                >  1:851815/1‑140 (MQ=255)                                                                                                                  tCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTGCGTGATTCAGGTCGATGATGTCGATACCGCGGCGGt                >  1:1471336/1‑140 (MQ=255)                                                                                                                  tCCGCCGTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTGCGTGATTCAGGTCGATGATGTCGATACCGCGGCGGt                >  2:1757274/1‑140 (MQ=255)                                                                                                                   ccgccgTGCCGTTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTGCGTGATTCAGGTCGATGATGTCGATACCGCGGCGGTa               >  1:107191/1‑140 (MQ=255)                                                                                                                         tgccgtTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTGCGTGATTCAGGTCGATGATGTCGATACCGCGGCGGTAGAAACg         >  2:368693/1‑140 (MQ=255)                                                                                                                          gccgtTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTGCGTGATTCAGGTCGATGATGTCGATACCGCGGCGGTAGAAACGc        >  2:1152926/1‑140 (MQ=255)                                                                                                                          gccgtTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTGCGTGATTCAGGTCGATGATGTCGATACCGCGGCGGTAGAAACGc        >  2:456526/1‑140 (MQ=255)                                                                                                                           ccgtTTCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTGCGTGATTCAGGTCGATGATGTCGATACCGCGGCGGTAGAAACGCt       <  2:851815/140‑1 (MQ=255)                                                                                                                              tttCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTGCGTGATTCAGGTCGATGATGTCGATACCGCGGCGGTAGAAACGCTTgg    >  1:927749/1‑140 (MQ=255)                                                                                                                                tCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTGCGTGATTCAGGTCGATGATGTCGATACCGCGGCGGTAGAAACGCTTGGTc  <  1:485705/140‑1 (MQ=255)                                                                                                                                tCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTGCGTGATTCAGGTCGATGATGTCGATACCGCGGCGGTAGAAACGCTTGGTc  <  1:449847/140‑1 (MQ=255)                                                                                                                                tCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTGCGTGATTCAGGTCGATGATGTCGATACCGCGGCGGTAGAAACGCTTGGTc  <  2:1112540/140‑1 (MQ=255)                                                                                                                                tCGCGATAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTGCGTGATTCAGGTCGATGATGTCGATACCGCGGCGGTAGAAACGCTTGGTc  >  2:826005/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                       GACTGACCAATAAGCGTGATATCCGCGTCAGCACCGCCAACGGGCGGATGGTTCTGACCGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCCGCCGTGCCGTTTCGCGCTAACAAAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCGCATTGCGTGATTCAGGTCGATGATGTCGATACCGCGGCGGTAGAAACGCTTGGTC  >  NC_000913/3995024‑3995290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑