Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,234,213	C→A	A169S (GCC→TCC)	nhaB ←	Na(+):H(+) antiporter NhaB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,234,213	0	C	A	100.0%	175.3 / NA	50	A169S (GCC→TCC)	nhaB	Na(+):H(+) antiporter NhaB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (30/20);  total (30/20)

CGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAAAGAGAGCGACAGCAGCATTTTGGAGC  >  NC_000913/1234078‑1234344                                                                                                                                        |                                                                                                                                    cgccTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATAcg                                                                                                                                 >  1:667193/1‑140 (MQ=255) cgccTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATAcg                                                                                                                                 <  2:280226/140‑1 (MQ=255)   ccTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATAcgcg                                                                                                                               <  2:523406/140‑1 (MQ=255)   ccTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTACCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATAcgcg                                                                                                                               <  2:584244/140‑1 (MQ=255)      aaTGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTg                                                                                                                            >  1:528535/1‑140 (MQ=255)            gTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTGATAAAt                                                                                                                      <  1:982252/140‑1 (MQ=255)            gTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTGATAAAt                                                                                                                      <  2:445349/140‑1 (MQ=255)            gTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACCGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTGATAAAt                                                                                                                      <  2:1504708/140‑1 (MQ=255)             tGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATAcgcg                                                                                                                               <  1:1138723/130‑1 (MQ=255)             tGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATAcgcg                                                                                                                               >  2:1138723/1‑130 (MQ=255)             tGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTGATAAATa                                                                                                                     >  1:1214938/1‑140 (MQ=255)             tGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTGATAAATa                                                                                                                     >  1:641911/1‑140 (MQ=255)                   cacCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTGATAAATACCATaa                                                                                                               <  2:1173266/140‑1 (MQ=255)                        gcatgcatCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTGATAAATACCATAAAAAcc                                                                                                          <  1:281811/140‑1 (MQ=255)                        gcatgcatCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTGATAAATACCATAAAAAcc                                                                                                          <  2:139022/140‑1 (MQ=255)                             catcatCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTGATAAATACCATAAAAACCGACTg                                                                                                     <  2:293896/140‑1 (MQ=255)                             catcatCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTGATAAATACCATAAAAACCGACTg                                                                                                     >  1:157795/1‑140 (MQ=255)                             catcatCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTGATAAATACCATAAAAACCGACTg                                                                                                     <  2:1630948/140‑1 (MQ=255)                               tcatcaGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACTGGAAGAGGATACGCGGTGATAAATACCATAAAAACCGACTGCa                                                                                                   <  1:1123257/140‑1 (MQ=255)                               tcatcaGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTGATAAATACCATAAAAACCGACTGCa                                                                                                   <  1:131786/140‑1 (MQ=255)                                catcaGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTGATAAATACCATAAAAACCGACTGCaa                                                                                                  <  2:244334/140‑1 (MQ=255)                                  tcaGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTGATAAATACCATAAAAACCGACTGCAACg                                                                                                <  1:270778/140‑1 (MQ=255)                                   caGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTGATAAATACCATAAAAACCGACTGCAACGc                                                                                               <  2:1070171/140‑1 (MQ=255)                                          cGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATcac                                                                                        >  1:354602/1‑140 (MQ=255)                                          cGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATcac                                                                                        >  2:1511030/1‑140 (MQ=255)                                          cGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATcac                                                                                        >  1:1378409/1‑140 (MQ=255)                                          cGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATca                                                                                         <  1:275408/139‑1 (MQ=255)                                          cGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATca                                                                                         >  2:275408/1‑139 (MQ=255)                                                aaGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATCACCACCGc                                                                                  >  1:429125/1‑140 (MQ=255)                                                           cTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATCACCACCGCCACGACGGTTa                                                                       >  2:487347/1‑140 (MQ=255)                                                                        aCTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTTTTCGGTACGGGAAGAGGATACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGaa                                                          >  1:1594447/1‑140 (MQ=255)                                                                        aCTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGaa                                                          >  1:613216/1‑140 (MQ=255)                                                                        aCTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGaa                                                          >  1:492657/1‑140 (MQ=255)                                                                        aCTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGaa                                                          >  2:125482/1‑140 (MQ=255)                                                                        aCTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGaa                                                          >  2:792135/1‑140 (MQ=255)                                                                                      gTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACg                                            >  1:325917/1‑140 (MQ=255)                                                                                      gTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACg                                            >  2:724264/1‑140 (MQ=255)                                                                                             tGACTTTCGTCTTGCGGGTCGGTGTCTTGGGTAGGGAAAGAGGATACGCGGTAAAAAATCCAAAAAAACCCGACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAgccgc                                     <  2:613216/140‑1 (MQ=255)                                                                                                 tATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTGATAAATACCAAAAAAACCGACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACg                                 <  1:1417989/140‑1 (MQ=255)                                                                                                     gTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCaaa                             >  2:1062624/1‑140 (MQ=255)                                                                                                     gTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCaaa                             >  1:206855/1‑140 (MQ=255)                                                                                                     gTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCaaa                             >  2:220433/1‑140 (MQ=255)                                                                                                     gTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCaaa                             >  2:594969/1‑140 (MQ=255)                                                                                                     gTCTTGCAGGGCGGTGTTTTCGGTACGGGAAGAGGATACGGGGTAAAAAATCCCAAAAAACCCCACTGCAACCCGCATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCaaa                             <  1:1475558/140‑1 (MQ=255)                                                                                                             ggTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAAAGAGAGc                     >  2:983987/1‑140 (MQ=255)                                                                                                             ggTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAAAGAGAGc                     >  1:1100988/1‑140 (MQ=255)                                                                                                              gTCGGTGTCTTCGGTACGGGAAGAGGATACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAAAGAGAGCg                    >  1:397169/1‑140 (MQ=255)                                                                                                                     tCTTCGGTACGGGAAGAGGATACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATAGAGGGACGCTGATAGGAACGCAACCGCCACACAAAAAGAGAACGAcagcag             >  1:1227379/1‑140 (MQ=255)                                                                                                                            tACGGGAAGAGGATACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAAAGAGAGCGAcagca              >  2:110077/1‑132 (MQ=255)                                                                                                                            tACGGGAAGAGGATACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAAAGAGAGCGAcagca              <  1:110077/132‑1 (MQ=255)                                                                                                                               tatcagagGATACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAAAGAGAGCGACAGCAGCATTTTGGAg   >  1:1651298/5‑140 (MQ=255)                                                                                                                               gggAAGAGGATACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAAAGAGAGCGACAGCAGCATTTTGGAg   >  1:1266473/1‑140 (MQ=255)                                                                                                                                ggAAGAGGATGCGGGGTGATAAATACCATAAAACCCGACTCCAACGCTGATCCCCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAAAGAGAGCGACAGCAGCATTTTGGAGc  <  1:487347/140‑1 (MQ=255)                                                                                                                                ggAAGAGGATAGGCGTTGATAAATACCAAAAAAACGGACTGCAACGCTGACCCCCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAAAGAGAGCGACAGCAGCATTTTGGAGc  <  1:125482/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    CGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGGTCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACCGACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAAAGAGAGCGACAGCAGCATTTTGGAGC  >  NC_000913/1234078‑1234344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑