Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,567,832	C→A	S92S (TCG→TCT)	eutH ←	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,567,832	0	C	A	100.0%	95.7 / NA	28	S92S (TCG→TCT)	eutH	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (17/11);  total (17/11)

GTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTC  >  NC_000913/2567707‑2567960                                                                                                                              |                                                                                                                                 gTTGGCCCCATCATCGACCCGTGAATTAACCCAGAGTTTACCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGcc                                                                                                                    <  2:732058/140‑1 (MQ=255)    ggCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGGGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAg                                                                                                                 <  1:602984/140‑1 (MQ=255)    ggCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAg                                                                                                                 >  1:102132/1‑140 (MQ=255)    ggCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAg                                                                                                                 >  1:647595/1‑140 (MQ=255)         catcatCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCAttt                                                                                                            >  1:317044/1‑140 (MQ=255)          atcatcGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTc                                                                                                           >  2:193284/1‑140 (MQ=255)             atcGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTa                                                                                                        <  2:400200/140‑1 (MQ=255)             atcGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTa                                                                                                        <  2:102132/140‑1 (MQ=255)                           tAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTa                                                                                          <  2:317044/140‑1 (MQ=255)                             aCCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTAcc                                                                                        >  1:554894/1‑140 (MQ=255)                                    gTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCga                                                                                 >  2:256816/1‑140 (MQ=255)                                        aGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGccc                                                                                   >  2:599796/1‑134 (MQ=255)                                        aGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGccc                                                                                   <  1:599796/134‑1 (MQ=255)                                             cgcgGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAg                                                                        >  1:121517/1‑140 (MQ=255)                                             cgcgGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAg                                                                        >  1:127387/1‑140 (MQ=255)                                              gcgGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGc                                                                       <  2:647595/140‑1 (MQ=255)                                               cgGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACg                                                                            >  1:457429/1‑134 (MQ=255)                                               cgGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACg                                                                            <  2:457429/134‑1 (MQ=255)                                               cgGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGcc                                                                      >  1:729942/1‑140 (MQ=255)                                                    aCGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTAc                                                                 <  2:121517/140‑1 (MQ=255)                                                    aCGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTAc                                                                 <  1:193284/140‑1 (MQ=255)                                                               cgccAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAgcg                                                      >  1:504709/1‑140 (MQ=255)                                                                            gCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGAc                                         <  2:127387/140‑1 (MQ=255)                                                                               aGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCAt                                      >  2:435826/1‑140 (MQ=255)                                                                                           cccATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAg                          >  1:251674/1‑140 (MQ=255)                                                                                                    cACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCAt                 >  2:311684/1‑140 (MQ=255)                                                                                                          agcagTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCAt           <  1:532815/140‑1 (MQ=255)                                                                                                                 ttCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCct    >  1:219169/1‑140 (MQ=255)                                                                                                                   ccGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCcttc  >  2:60144/1‑140 (MQ=255)                                                                                                                              |                                                                                                                                 GTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTC  >  NC_000913/2567707‑2567960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑