BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	634,335	G→A	A250A (GCG→GCA)	ybdL →	methionine‑oxo‑acid transaminase, PLP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	634,335	0	G	A	100.0%	86.8 / NA	27	A250A (GCG→GCA)	ybdL	methionine‑oxo‑acid transaminase, PLP‑dependent
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (9/18); total (9/18)

ACGAGCACATCAACTTTTCACAACAGGGCCATGCCAGTGTGCTGGCGCATCCGCAGCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCG‑AAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAATACCCCGGCACAGCTGGCGCTTGCTGATATGCTACGTGCAGAACCTGAGCATTATCTTGCGTTACCGGACTTTTAT  >  NC_000913/634199‑634470

aCGAGCACATCAACTTTTCACAACAGGGCCATGCCAGTGTGCTGGCGCATCCGCAGCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCACCa                                                                                                                                       <  2:92899/140‑1 (MQ=255)

       caTCAACTTTTCACAACAGGGCCATGCCAGTGTGCTGGCGCATCCGCAGCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCACCAGCGCCCa                                                                                                                                >  1:147332/1‑140 (MQ=255)

                 tCACAACAGGGCCATGCCAGTGTGCTGGCGCATCCGCAGCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCACCAGCGCCCATCAGCGCCG‑a                                                                                                                     <  1:248375/140‑1 (MQ=255)

                  cacaACAGGGCCATGCCAGTGTGCTGGCGCATCCGCAGCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCCAGACCCATCATATGACCGGCTGGAAAGGGGGGTATTTTGTTGCACCAGCGCCCATCAGCGCCG‑aa                                                                                                                    >  2:133476/1‑140 (MQ=255)

                     gacaGGGCCATGCCAGTGTGCTGGCGCATCCGCAGCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCACCAGCGCCCATCAGCGCCG‑AAAtt                                                                                                                 <  1:292428/139‑1 (MQ=255)

                     aacaGGGCCATGCCAGTGTGCTGGCGCATCCGCAGCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGGAAGAGGGTTATTTTGTTTCAACAGCCCCCATTAACGCCG‑GAAtt                                                                                                                 >  2:110301/1‑140 (MQ=255)

                       caGGGCCATGCCAGTGTGCTGGCGCATCCGCAGCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCACCAGCGCCCATCAGCGCCG‑AAATTCg                                                                                                               <  2:638875/140‑1 (MQ=255)

                         gggCCATGCCAGTGTGCTGGCGCATCCGCAGCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCACCAGCGCCCATCAGCGCCG‑AAATTCGCa                                                                                                             <  1:110301/140‑1 (MQ=255)

                          tgCCATGCCAGTGTGCTGGCGCATCCGCAGCTGCGTGAGCGGGCAGTGGCGGATTCTTTATTTTGGAAGACCTATCATATTACCGGATGGAAATGGGGTTATTGTGTTGTCCCAGCGCCCAACAGCGCCGCAAAttgaaa                                                                                                             >  2:552334/2‑136 (MQ=255)

                          ggCCATGCCAGTGTGCTGGCGCATCCGCAGCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGGAAGGGGGGTATTGTGTTGCACCAGCGCCCATCAGCCCCG‑AAAATCGCaa                                                                                                            >  2:314779/1‑140 (MQ=255)

                            ccATGCCAGTGTGCTGGCGCATCCGCAGCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCACCAGCGCCCATCAGCGCCG‑AAATTCGCAAgg                                                                                                          <  2:543619/140‑1 (MQ=255)

                             cATGCCAGTGTGCTGGCGCATCCGCAGCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCACCAGCGCCCATCAGCGCCG‑AAATTCGCAAGGt                                                                                                         <  1:722159/140‑1 (MQ=255)

                             cATGCCAGTGTGCTGGCGCATCCGCAGCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCACCAGCGCCCATCAGCGCCG‑AAATTCGCAAGGt                                                                                                         <  2:147332/140‑1 (MQ=255)

                               tGCCAGTGTGCTGGCGCATCCGCAGCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGGGGGGTATTGTGTTGGACCAGCGCCCCTCAGCGCCG‑AAAATCGCAAGGTAc                                                                                                       >  2:669281/1‑140 (MQ=255)

                                     tgtgCTGGCGCATCCGCAGCTGCGTGAGCGGGCAGTGGCGGTTTCTTTATTTTGCAAGACCTATCATATTACCGGCTGGAAAGGGGGGTATTGTGTTGCACCAGCGCCCATCAGCGCCG‑AAATTCGCAAGGGACATCAGt                                                                                                 >  2:295541/1‑140 (MQ=255)

                                           ggCGCATCCGCAGCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCACCAGCGCCCATCAGCGCCG‑AAATTCGCAAGGTACATCAGTATCTGa                                                                                           <  2:722804/140‑1 (MQ=255)

                                                   cGCAGCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCACCAGCGCCCATCAGCGCCG‑AAATTCGCAAGGTACATCAGTATCTGACCTTTTct                                                                                   <  1:516558/140‑2 (MQ=255)

                                                           cGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCACCAGCGCCCATCAGCGCCG‑AAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAATAc                                                                           >  1:754875/1‑140 (MQ=255)

                                                             tGAGCGGGCAGTGGCGGTTTCTTCATTTTGCAAGACCCATCATATGACCGGCTGGGAAGTGGGGTATTGTGTTGCACCCGCGCCCCTCAGCGCCG‑AAATTCGCAAGGGACATCAGGATCTGACCTTTTTTGTGAATAccc                                                                         >  2:516558/1‑140 (MQ=255)

                                                                gCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCACCAGCGCCCATCAGCGCCG‑AAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAATACCCCgg                                                                      <  1:552334/140‑1 (MQ=255)

                                                                               ttcttcATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCACCAGCGCCCATCAGCGCCG‑AAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAATACCCCGGCACAGCTGGCGCTTg                                                       <  1:258819/140‑1 (MQ=255)

                                                                                    cATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCACCAGCGCCCATCAGCGCCG‑AAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAATACCCCGGCACAGCTGGCGCTTGCTGat                                                  <  1:314779/140‑1 (MQ=255)

                                                                                      tttGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCACCAGCGCCCATCAGCGCCG‑AAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAATACCCCGGCACAGCTGGCGCTTGCTGatat                                                <  1:133476/140‑1 (MQ=255)

                                                                                         ggCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCACCAGCGCCCATCAGCGCCG‑AAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAATACCCCGGCACAGCTGGCGCTTGCTGATATGCt                                             <  1:669281/140‑1 (MQ=255)

                                                                                                   aTCATATGACCGGCTGTAAAGTGGGTTATTGTGTTGCACCAGCGCCCATCAGCGCCG‑AAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAATACCCCGGCACAGCTGGCGCTTGCTGATATGCTACGTGCAGaa                                   >  2:542621/1‑140 (MQ=255)

                                                                                                      atatGACCGGCTGGAAAGTGGGTTATTGTGTTGCACCAGCGCCCATCAGCGCCG‑AAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAATACCCCGGCACAGCTGGCGCTTGCTGATATGCTACGTGCAGAACCt                                <  1:181093/140‑1 (MQ=255)

                                                                                                          gACCGGCTGGAAAGTGGGTTATTGTGTTGCACCAGCGCCCATCAGCGCCG‑AAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAATACCCCGGCACAGCTGGCGCTTGCTGATATGCTACGTGCAGAACCTGAGc                            >  2:109560/1‑140 (MQ=255)

                                                                                                          gACCGGCTGGAAAGTGGGTTATTGTGTTGCACCAGCGCCCATCAGCGCCG‑AAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAATACCCCGGCACAGCTGGCGCTTGCTGATATGCTACGTGCAGAACCTGAGc                            <  2:754875/140‑1 (MQ=255)

                                                                                                           aCCGGCTGGAAAGTGGGTTATTGTGTTGCACCAGCGCCCATCAGCGCCG‑AAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAATACCCCGGCACAGCTGGCGCTTGCTGATATGCTACGTGCAGAACCTGAGCa                           >  1:326510/1‑140 (MQ=255)

                                                                                                             cGGCTGGAAAGTGGGTTATTGTGTTGCACCAGCGTCCATCAGCGCCG‑AAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAATACCCCGGCACAGCTGGCGCTTGCTGATATGCTACGTGCAGAACCTGAGCAtt                         >  1:64695/1‑140 (MQ=255)

                                                                                                               gCTGGAAAGTGGGTTATTGTGTTGCACCAGCGCCCATCAGCGCCG‑AAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAATACCCCGGCACAGCTGGCGCTTGCTGATATGCTACGTGCAGAACCTGAGCATTAt                       >  1:557800/1‑140 (MQ=255)

                                                                                                                cTGGAAAGTGGGTTATTGTGTTGCACCAGCGCCCATCAGCGCCG‑AAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAATACCCCGGCACAGCTGGCGCTTGCTGATATGCTACGTGCAGAAc                                  >  2:107996/1‑128 (MQ=255)

                                                                                                                cTGGAAAGTGGGTTATTGTGTTGCACCAGCGCCCATCAGCGCCG‑AAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAATACCCCGGCACAGCTGGCGCTTGCTGATATGCTACGTGCAGAAc                                  <  1:107996/128‑1 (MQ=255)

                                                                                                                                  tgtTGCACCAGCGCCCATCAGCGCCG‑AAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAATACCCCGGCACAGCTGGCGCTTGCTGATATGCTACGTGCAGAACCTGAGCATTATCTTGCGTTACCGGACtttt    >  2:576937/1‑140 (MQ=255)

                                                                                                                                    ttGCACCAGCGCCCATCAGCGCCG‑AAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAATACCCCGGCACAGCTGGCGCTTGCTGATATGCTACGTGCAGAACCTGAGCATTATCTTGCGTTACCGGACTTTTAt  >  1:637115/1‑140 (MQ=255)

ACGAGCACATCAACTTTTCACAACAGGGCCATGCCAGTGTGCTGGCGCATCCGCAGCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCG‑AAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAATACCCCGGCACAGCTGGCGCTTGCTGATATGCTACGTGCAGAACCTGAGCATTATCTTGCGTTACCGGACTTTTAT  >  NC_000913/634199‑634470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`