Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,629,295	G→T	R82S (CGC→AGC)	rbbA ←	ribosome‑associated ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,629,295	0	G	T	100.0%	78.3 / NA	23	R82S (CGC→AGC)	rbbA	ribosome‑associated ATPase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (11/12);  total (11/12)

CCCGCTCCGCTTTGTCGTGACCGAACAGGCGAGCGAAAAAATCGACGTTTTCATACACCGACAAGGTGTGGTAGAGGTTTTTGCCCAGCCCCTGCGGCATCCAGGCGATGCGCGGGCAGACGTCGCGGCGATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGCCCTGTTCAATGACGCGGGCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGC  >  NC_000913/3629166‑3629430                                                                                                                                  |                                                                                                                                        cccGCTCCGCTTTGTCGTGACCGAACAGGCGAGCGAAAAAATCGACGTTTTCATACACCGACAAGGTGTGGTAGAGGTTTTTGCCCAGCCCCTGCGGCATCCAGGCGATGCGCGGGCAGACGTCGCGGCTATGCTTCggg                                                                                                                               <  1:102258/140‑1 (MQ=255) cccGCTCCGCTTTGTCGTGACCGAACAGGCGAGCGAAAAAATCGACGTTTTCATACACCGACAAGGTGTGGTAGAGGTTTTTGCCCAGCCCCTGCGGCATCCAGGCGATGCGCGGGCAGACGTCGCGGCTATGCTTCggg                                                                                                                               >  2:274286/1‑140 (MQ=255)  ccgctccgctTTGTCGTGACCGAACAGGCGAGCGAAAAAATCGACGTTTTCATACACCGACAAGGTGTGGTAGAGGTTTTTGCCCAGCCCCTGCGGCATCCAGGCGATGCGCGGGCAGACGTCGCGGCTATGCTTCGGGt                                                                                                                              <  1:519243/140‑1 (MQ=255)            ttGTCGTGACCGAACAGGCGAGCGAAAAAATCGACGTTTTCATACACCGACAAGGTGTGGTAGAGGTTTTTGCCCAGCCCCTGCGGCATCCAGGCGATGCGCGGGCAGACGTCGCGGCTATGCTTCGGGTCGCGCATATc                                                                                                                    >  1:442044/1‑140 (MQ=255)                   gACCGAACAGGCGAGCGAAAAAATCGACGTTTTCATACACCGACAAGGTGTGGTAGAGGTTTTTGCCCAGCCCCTGCGGCATCCAGGCGATGCGCGGGCAGACGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGccc                                                                                                             <  2:442044/140‑1 (MQ=255)                         aCAGGCGAGCGAAAAAATCGACGTTTTCATACACCGACAAGGTGTGGTAGAGGTTTTTGCCCAGCCCCTGCGGCATCCAGGCGATGCGCGGGCAGATGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGCCCAGCAcc                                                                                                       >  2:20743/1‑140 (MQ=255)                                    aaaaaaTCGACGTTTTCATACACCGACAAGGTGTGGTAGAGGTTTTTGCCCAGCCCCTGCGGCATCCAGGCGATGCGCGGGCAGACGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGcc                                                                                            <  2:400962/140‑1 (MQ=255)                                    aaaaaaTCGACGTTTTCATACACCGACAAGGTGTGGTAGAGGTTTTTGCCCAGCCCCTGCGGCATCCAGGCGATGCGCGGGCAGACGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGcc                                                                                            <  1:409982/140‑1 (MQ=255)                                    aaaaaaTCGACGTTTTCATACACCGACAAGGTGTGGTAGAGGTTTTTGCCCAGCCCCTGCGGCATCCAGGCGATGCGCGGGCAGACGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGcc                                                                                            <  1:274286/140‑1 (MQ=255)                                       aaaTCGACGTTTTCATACACCGACAAGGTGTGGTAGAGGTTTTTGCCCAGCCCCTGCGGCATCCAGGCGATGCGCGGGCAGACGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGCCCTg                                                                                         >  2:55913/1‑140 (MQ=255)                                             aCGTTTTCATACACCGACAAGGTGTGGTAGAGGTTTTTGCCCAGCCCCTGCGGCATCCAGGCGATGCGCGGGCAGACGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGCCCTGTTCAAt                                                                                   <  2:429305/140‑1 (MQ=255)                                                          ccGACAAGGTGTGGTAGAGGTTTTTGCCCAGCCCCTGCGGCATCCAGGCGATGCGCGGGCAGACGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGCCCTGTTCAATGACGCGGGCACCg                                                                      >  2:310415/1‑140 (MQ=255)                                                             aCAAGGTGTGGTAGAGGTTTTTGCCCAGCCCCTGCGGCATCCAGGCGATGCGCGGGCAGACGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGCCCTGTTCAATGACGCGGGCACCGGaa                                                                   >  1:694896/1‑140 (MQ=255)                                                                                       aGCCCCTGCGGCATCCAGGCGATGCGCGGGCAGACGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGCCCTGTTCAATGACGCGGGCACCGGAAATCAACGaca                                                         <  1:520543/124‑1 (MQ=255)                                                                                       aGCCCCTGCGGCATCCAGGCGATGCGCGGGCAGACGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGCCCTGTTCAATGACGCGGGCACCGGAAATCAACGaca                                                         >  2:520543/1‑124 (MQ=255)                                                                                            cTGCGGCATCCAGGCGATGCGCGGGCAGACGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGCCCTGTTCAATGACGCGGGCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGc                                    >  2:354774/1‑140 (MQ=255)                                                                                                   aTCCAGGCGATGCGCGGGCAGACGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGCCCTGTTCAATGACGCGGGCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCgg                             >  2:302584/1‑140 (MQ=255)                                                                                                         gCGATGCGCGGGCAGACGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGCCCTGTTCAATGACGCGGGCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAAt                       <  1:310415/140‑1 (MQ=255)                                                                                                            aTGCGCGGGCAGACGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGCCCTGTTCAATGACGCGGGCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAg                    <  2:694896/140‑1 (MQ=255)                                                                                                             tGCGCGGGCAGACGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGCCCTGTTCAATGACGCGGGCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGc                   >  1:165879/1‑140 (MQ=255)                                                                                                               cgcgGGCAGACGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGCCCTGTTCAATGACGCGGGCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGccc                 <  2:165879/140‑1 (MQ=255)                                                                                                               cgcgGGCAGACGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGCCCTGTTCAATGACGCGGGCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGccc                 >  2:132037/1‑140 (MQ=255)                                                                                                                         cGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGCCCTGTTCAATGACGCGGGCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAg       <  1:354774/140‑1 (MQ=255)                                                                                                                             gcggctATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGCCCTGTTCAATGACGCGGGCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCggg   <  2:247288/140‑1 (MQ=255)                                                                                                                              cggctATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGCCCTGTTCAATGACGCGGGCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGc  >  2:36796/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                        CCCGCTCCGCTTTGTCGTGACCGAACAGGCGAGCGAAAAAATCGACGTTTTCATACACCGACAAGGTGTGGTAGAGGTTTTTGCCCAGCCCCTGCGGCATCCAGGCGATGCGCGGGCAGACGTCGCGGCGATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGCCCTGTTCAATGACGCGGGCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGC  >  NC_000913/3629166‑3629430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑