Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,411,207 G→A A219T (GCC→ACC)  yjfJ → putative transcriptional regulator effector protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,411,2070GA92.3% 65.8 / ‑0.8 26A219T (GCC→ACC) yjfJputative transcriptional regulator effector protein
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (7/17);  total (8/18)
Fisher's exact test for biased strand distribution p-value = 5.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.94e-01

TAAAACGCCTGCAAACGCGTCAGGCCGAACGTCAGGCTCGCCTGGATGCTGCCGCACAGTTGGAGAAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCGCCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGTAATTTTTTTGCCAGCCTCGCTAAAAGGCTGGCAACTATTTTAAGGATAAAATATGTCTGGTTTTTTCCAGCGTCTGTTTGGCAA  >  NC_000913/4411070‑4411333
                                                                                                                                         |                                                                                                                              
tAAAACGCCTGCAAACGCGTCAGGCCGAACGTCAGGCTCGCCTGGATGCTGCCGCACAGTTGGAGAAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCAcc                                                                                                                              <  1:639476/140‑1 (MQ=255)
   aaCGCCTGCAAACGCGTCAGGCCGAACGTCAGGCTCGCCTGGATGCTGCCGCACAGTTGGAGAAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAg                                                                                                                           <  1:668727/140‑1 (MQ=255)
    aCGCCTGCAAACGCGTCAGGCCGAACGTCAGGCTCGCCTGGATGCTGCCGCACAGTTGGAGAAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAgg                                                                                                                          <  1:153766/140‑1 (MQ=255)
    aCGCCTGCAAACGCGTCAGGCCGAACGTCAGGCTCGCCTGGATGCTGCCGCACAGTTGGAGAAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAgg                                                                                                                          <  2:108796/140‑1 (MQ=255)
      gCCTGCAAACGCGTCAGGCCGAACGTCAGGCTAGCCTGGATGCTGCCGCACAGTTGGAGAAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGAt                                                                                                                        <  1:182862/140‑1 (MQ=255)
       ccTGCAAACGCGTCAGGCCGAACGTCAGGCTCGCCTGGATGCTGCCGCACAGTTGGAGAAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGGAGCACCCAGGATg                                                                                                                       >  2:289407/1‑140 (MQ=255)
        cTGCAAACGCGTCAGGCCGAACGTCAGGCTCGCCTGGATGCTGCCGCACAGTTGGAGAAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGATGt                                                                                                                      >  2:514058/1‑140 (MQ=255)
             aaCGCGTCAGGCCGAACGTCAGGCTCGCCTGGATGCTGCCGCACAGTTGGAGAAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGATGTATTAg                                                                                                                 <  1:484547/140‑1 (MQ=255)
                      ggCCGAACGTCAGGCTCGCCTGGATGCTGCCGCACAGTTGGAGAAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGATGTATTAGCAAGACTGc                                                                                                        <  1:26262/140‑1 (MQ=255)
                        ccGAACGTCAGGCTCGCCTGGATGCTGCCGCACAGTTGGAGAAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGATGTATTAGCAAGACTGCaa                                                                                                      <  1:514058/140‑1 (MQ=255)
                                        ccTGGATGCTGCCGCACAGTTGGAGAAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGt                                                                                      >  1:469730/1‑140 (MQ=255)
                                                 tgcCGCACAGTTGGAGAAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCGCCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGTAAttttttt                                                                             >  1:322181/1‑140 (MQ=255)
                                                   ccGCACAGTTGGAGAAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGTAATTTTTTTGc                                                                           >  1:343898/1‑140 (MQ=255)
                                                       acaGTTGGAGAAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGTAATTTTTTTGCCAGc                                                                       <  2:343898/140‑1 (MQ=255)
                                                           ttGGAGAAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGTAATTTTTTTGCCAGCCTCg                                                                   >  1:709695/1‑140 (MQ=255)
                                                                gaAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGTAATTTTTTTGCCAGCCTCGCTaaa                                                              <  2:709695/140‑1 (MQ=255)
                                                                gaAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGTAATTTTTTTGCCAGCCTCGCTaaa                                                              <  2:351130/140‑1 (MQ=255)
                                                                     tCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGTAATTTTTTTGCCAGCCTCGCTaa                                                               >  1:290481/1‑134 (MQ=255)
                                                                     tCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGTAATTTTTTTGCCAGCCTCGCTaa                                                               <  2:290481/134‑1 (MQ=255)
                                                                     tCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGTAATTTTTTTGCCAGCCTCGCTAAAAGGCt                                                         <  1:358589/140‑1 (MQ=255)
                                                                     tCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAAGAGGACCCAGGGTGTATTAGCAAGACTGCAACGGCAAAAAGGGGAGGAATTTTTTTTGCCGCCTCGCTAAAAgggt                                                         >  2:524181/1‑138 (MQ=255)
                                                                        cAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGTAATTTTTTTGCCAGCCTCGCTAAAAGGCTGGc                                                      <  2:469730/140‑1 (MQ=255)
                                                                         aGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGTAATTTTTTTGCCAGCCTCGCTAAAAGGCTGGCa                                                     <  2:74511/140‑1 (MQ=255)
                                                                                             aaaaGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGTAATTTTTTTGCCAGCCTCGCTAAAAGGCTGGCAACTATTTTAAGGATAAAata                                 >  1:517825/1‑140 (MQ=255)
                                                                                                     gCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGTAATTTTTTTGCCAGCCTCGCTAAAAGGCTGGCAACTATTTTAAGGAt                                       <  2:506934/126‑1 (MQ=255)
                                                                                                     gCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGTAATTTTTTTGCCAGCCTCGCTAAAAGGCTGGCAACTATTTTAAGGAt                                       >  1:506934/1‑126 (MQ=255)
                                                                                                                          aGCAATAAAAGTAGCACCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGTAATTTTTTTGCCAGCCTCGCTAAAGGGCTGGCAACTATTTTAAGGATAAAATATGTCTGGTTTTTTCCAGCGTCTGTTTGGc    <  1:524181/140‑1 (MQ=255)
                                                                                                                            cAATAAAAGTAGCGCCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGTAATTTTTTTGCCAGCCTCGCTAAAAGGCTGGCAACTATTTTAAGGATAAAATATGTCTGGTTTTTTCCAGCGTCTGTTTGGCaa  <  2:322181/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                              
TAAAACGCCTGCAAACGCGTCAGGCCGAACGTCAGGCTCGCCTGGATGCTGCCGCACAGTTGGAGAAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCGCCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGTAATTTTTTTGCCAGCCTCGCTAAAAGGCTGGCAACTATTTTAAGGATAAAATATGTCTGGTTTTTTCCAGCGTCTGTTTGGCAA  >  NC_000913/4411070‑4411333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑