Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,951,592	G→T	A66A (GCG→GCT)	yecE →	DUF72 domain‑containing protein YecE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,951,592	0	G	T	100.0%	87.2 / NA	26	A66A (GCG→GCT)	yecE	DUF72 domain‑containing protein YecE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (12/14);  total (12/14)

TGAAGAGTATGCCCGCCACTTTAACTGCGTGGAGGGCAACACCACGCTTTACGCCCTGCCGAAACCCGAGGTTGTCCTGCGCTGGCGTGAGCAGACCACAGATGACTTCCGCTTCTGTTTTAAGTTTCCGGCGACCATTTCGCATCAGGCAGCATTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATT  >  NC_000913/1951460‑1951711                                                                                                                                     |                                                                                                                        tGAAGAGTATGCCCGCCACTTTAACTGCGTGGAGGGCAACACCACGCTTTACGCCCTGCCGAAACCCGAGGTTGTCCTGCGCTGGCGTGAGCAGACCACAGATGACTTCCGCTTCTGTTTTAAGTTTCCGGCTACCAttt                                                                                                                  <  1:651662/140‑1 (MQ=255)     gagTATGCCCGCCACTTTAACTGCGTGGAGGGCAACACCACGCTTTACGCCCTGCCGAAACCCGAGGTTGTCCTGCGCTGGCGTGAGCAGACCACAGATGACTTCCGCTTCTGTTTTAAGTTTCCGGCTACCATTTCGCa                                                                                                              <  1:636146/140‑1 (MQ=255)        tATGCCCGCCACTTTAACTGCGTGGAGGGCAACACCACGCTTTACGCCCTGCCGAAACCCGAGGTTGTCCTGCGCTGGCGTGAGCAGACCACAGATGACTTCCGCTTCTGTTTTAAGTTTCCGGCTACCATTTCGCATCa                                                                                                           >  2:122925/1‑140 (MQ=255)        tATGCCCGCCACTTTAACTGCGTGGAGGGCAACACCACGCTTTACGCCCTGCCGAAACCCGAGGTTGTCCTGCGCTGGCGTGAGCAGACCACAGATGACTTCCGCTTCTGTTTTAAGTTTCCGGCTACCATTTCGCATCa                                                                                                           >  1:531457/1‑140 (MQ=255)                ccACTTTAACTGCGTGGAGGGCAACACCACGCTTTACGCCCTGCCGAAACCCGAGGTTGTCCTGCGCTGGCGTGAGCAGACCACAGATGACTTCCGCTTCTGTTTTAAGTTTCCGGCTACCATTTCGCa                                                                                                              <  2:499831/129‑1 (MQ=255)                ccACTTTAACTGCGTGGAGGGCAACACCACGCTTTACGCCCTGCCGAAACCCGAGGTTGTCCTGCGCTGGCGTGAGCAGACCACAGATGACTTCCGCTTCTGTTTTAAGTTTCCGGCTACCATTTCGCa                                                                                                              >  1:499831/1‑129 (MQ=255)                ccACTTTAACTGCGTGGAGGGCAACACCACGCTTTACGCCCTGCCGAAACCCGAGGTTGTCCTGCGCTGGCGTGAGCAGACCACAGATGACTTCCGCTTCTGTTTTAAGTTTCCGGCTACCATTTCGCATCAGGCAGCAt                                                                                                   >  2:142113/1‑140 (MQ=255)                ccACTTTAACTGCGTGGAGGGCAACACCACGCTTTACGCCCTGCCGAAACCCGAGGTTGTCCTGCGCTGGCGTGAGCAGACCACAGATGACTTCCGCTTCTGTTTTAAGTTTCCGGCTACCATTTCGCATCAGGCAGCAt                                                                                                   >  1:230037/1‑140 (MQ=255)                  aCTTTAACTGCGTGGAGGGCAACACCACGCTTTACGCCCTGCCGAAACCCGAGGTTGTCCTGCGCTGGCGTGAGCAGACCACAGATGACTTCCGCTTCTGTTTTAAGTTTCCGGCTACCATTTCGCATCAGGCAGCATTa                                                                                                 >  1:511851/1‑140 (MQ=255)                       aaCTGCGTGGAGGGCAACACCACGCTTTACGCCCTGCCGAAACCCGAGGTTGTCCTGCGCTGGCGTGAGCCGACCACAGATGACTTCCGCTTCTGTTTTAAGTTTCCGGCTACCATTTCGCATCAGGCAGCATTACGGCa                                                                                            <  2:121685/140‑1 (MQ=255)                       aaCTGCGTGGAGGGCAACACCACGCTTTACGCCCTGCCGAAACCCGAGGTTGTCCTGCGCTGGCGTGAGCAGACCACAGATGACTTCCGCTTCTGTTTTAAGTTTCCGGCTACCATTTCGCATCAGGCAGCATTACGGCa                                                                                            <  1:142113/140‑1 (MQ=255)                       aaCTGCGTGGAGGGCAACACCACGCTTTACGCCCTGCCGAAACCCGAGGTTGTCCTGCGCTGGCGTGAGCAGACCACAGATGACTTCCGCTTCTGTTTTAAGTTTCCGGCTACCATTTCACATCAGGCAGCATTACGGCa                                                                                            <  2:376298/140‑1 (MQ=255)                          tGCGTGGAGGGCAACACCACGCTTTACGCCCTGCCGAAACCCGAGGTTGTCCTGCGCTGGCGTGAGCAGACCACAGATGACTTCCGCTTCTGTTTTAAGTTTCCGGCTACCATTTCGCATCAGGCAGCATTACGGCATTg                                                                                         <  1:122925/140‑1 (MQ=255)                            cGTGGAGGGCAACACCACGCTTTACGCCCTGCCGAAACCCGAGGTTGTCCTGCGCTGGCGTGAGCAGACCACAGATGACTTCCGCTTCTGTTTTAAGTTTCCGGCTACCATTTCGCATCAGGCAGCATTACGGCATTGCg                                                                                       <  2:511851/140‑1 (MQ=255)                            cGTGGAGGGCAACACCACGCTTTACGCCCTGCCGAAACCCGAGGTTGTCCTGCGCTGGCGTGAGCAGACCACAGATGACTTCCGCTTCTGTTTTAAGTTTCCGGCTACCATTTCGCATCAGGCAGCATTACGGCATTGCg                                                                                       <  2:701759/140‑1 (MQ=255)                             gTGGAGGGCAACACCACGCTTTACGCCCTGCCGAAACCCGAGGTTGTCCTGCGCTGGCGTGAGCAGACCACAGATGACTTCCGCTTCTGTTTTAAGTTTCCGGCTACCATTTCGCATCAGGCAGCATTACGGCATTGCga                                                                                      >  1:725968/1‑140 (MQ=255)                                                                     aGGTTGTCCTGCGCTGGCGTGAGCAGACCACAGATGACTTCCGCTTCTGTTTTAAGTTTCCGGCTACCATTTCGCATCAGGCAGCATTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTg                                              >  1:549698/1‑140 (MQ=255)                                                                               gcgcTGGCGTGAGCAGACCACAGATGACTTCCGCTTCTGTTTTAAGTTTCCGGCTACCATTTCGCATCAGGCAGCATTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCa                                    <  1:313132/140‑1 (MQ=255)                                                                               gcgcTGGCGTGAGCAGACCACAGATGACTTCCGCTTCTGTTTTAAGTTTCCGGCTACCATTTCGCATCAGGCAGCATTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCa                                    <  2:682033/140‑1 (MQ=255)                                                                               gcgcTGGCGTGAGCAGACCACAGATGACTTCCGCTTCTGTTTTAAGTTTCCGGCTACCATTTCGCATCAGGCAGCATTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCa                                    <  2:725968/140‑1 (MQ=255)                                                                                   tGGCGTGAGCAGACCACAGATGACTTCCGCTTCTGTTTTAAGTTTCCGGCTACCATTTCGCATCAGGCAGCATTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTgg                                <  2:531457/140‑1 (MQ=255)                                                                                         gAGCAGACCACAGATGACTTCCGCTTCTGTTTTAAGTTTCCGGCTACCATTTCGCATCAGGCAGCATTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATa                          <  1:144806/140‑1 (MQ=255)                                                                                          aGCAGACCACAGATGACTTCCGCTTCTGTTTTAAGTTTCCGGCTACCATTTCGCATCAGGCAGCATTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATAc                         >  2:744052/1‑140 (MQ=255)                                                                                               aCCACAGATGACTTCCGCTTCTGTTTTAAGTTTCCGGCTACCATTTCGCATCAGGCAGCATTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCt                    >  2:518332/1‑140 (MQ=255)                                                                                                ccACAGATGACTTCCGCTTCTGTTTTAAGTTTCCGGCTACCATTTCGCATCAGGCAGCATTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTg                   >  2:144416/1‑140 (MQ=255)                                                                                                      aTGACTTCCGCTTCTGTTTTAAGTTTCCGGCTACCATTTCGCATCAGGCAGCATTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAActg             >  2:491369/1‑140 (MQ=255)                                                                                                                 ttCTGTTTTAAGTTTCCGGCTACCATTTCGCATCAGGCAGCATTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCCCCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACAtt  <  2:714937/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                        TGAAGAGTATGCCCGCCACTTTAACTGCGTGGAGGGCAACACCACGCTTTACGCCCTGCCGAAACCCGAGGTTGTCCTGCGCTGGCGTGAGCAGACCACAGATGACTTCCGCTTCTGTTTTAAGTTTCCGGCGACCATTTCGCATCAGGCAGCATTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATT  >  NC_000913/1951460‑1951711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑