New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3888183	45 (0.870)	3 (0.060) +GGTAA	3/254	5.0	6.1%	coding (1356/1365 nt)	mnmE	5‑carboxymethylaminomethyluridine‑tRNA synthase GTPase subunit
	?	NC_000913	3888184 =	49 (0.910)					coding (1357/1365 nt)	mnmE	5‑carboxymethylaminomethyluridine‑tRNA synthase GTPase subunit
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GTTGGGAGCCTGGGCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATT  >  NC_000913/3888057‑3888322                                                                                                                                     |                                                                                                                                           gTTGGGAGCCTGGGCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACcgc                                                                                                                                <  2:799821/140‑1 (MQ=255)        gCCTGGGCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACg                                                                                                                         <  2:112694/140‑1 (MQ=255)         ccTGGGCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGa                                                                                                                        >  2:564977/1‑140 (MQ=255)         ccTGGGCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGa                                                                                                                        >  1:603339/1‑140 (MQ=255)         ccTGGGCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGa                                                                                                                        >  1:894571/1‑140 (MQ=255)           tGGGCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAg                                                                                                                      >  1:486770/1‑140 (MQ=255)            gggCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGc                                                                                                                     >  2:240311/1‑140 (MQ=255)            gggCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGc                                                                                                                     <  1:906633/140‑1 (MQ=255)                  gTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCAtt                                                                                                               >  1:497279/1‑140 (MQ=255)                       ctgctgGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGGTAAGTAACCGCGCTTACGAAGCCGCAtt                                                                                                               >  2:666461/1‑140 (MQ=255)                       ctgctgGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGAc                                                                                                          >  2:845352/1‑140 (MQ=255)                            ggCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCa                                                                                                     >  1:168024/1‑140 (MQ=255)                                  agagTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCg                                                                                               >  1:829859/1‑140 (MQ=255)                                   gagTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCgg                                                                                              <  1:412636/140‑1 (MQ=255)                                    agTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGAt                                                                                                  >  2:776346/1‑140 (MQ=255)                                     gTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgct                                                                                            <  1:775755/140‑1 (MQ=255)                                      ttGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACTACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgctt                                                                                           <  1:262521/140‑1 (MQ=255)                                        gCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcg                                                                                         <  2:829859/140‑1 (MQ=255)                                         cggcTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcgc                                                                                        <  2:611353/137‑1 (MQ=255)                                         cGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcgc                                                                                        <  1:231383/140‑1 (MQ=255)                                         cGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcgc                                                                                        <  1:804982/140‑1 (MQ=255)                                           tCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcgctt                                                                                      <  1:27603/140‑1 (MQ=255)                                            cTGGCTCAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCTCATTCTGACTGTCAGATGCGgcttcgcttc                                                                                     <  2:857576/140‑1 (MQ=255)                                             tGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCa                                                                                    <  2:478035/140‑1 (MQ=255)                                              ggCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCAt                                                                                   <  1:564977/140‑1 (MQ=255)                                                cacaGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTg                                                                                 >  2:463946/1‑140 (MQ=255)                                                    gcagAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTAc                                                                             <  1:845352/140‑1 (MQ=255)                                                    gcagAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTAc                                                                             <  2:185105/140‑1 (MQ=255)                                                    gcagAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTAc                                                                             <  2:289900/140‑1 (MQ=255)                                                     cagAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTAcc                                                                            <  2:894571/140‑1 (MQ=255)                                                     cagAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTAcc                                                                            <  1:582631/140‑1 (MQ=255)                                                      agAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCa                                                                           <  2:766693/140‑1 (MQ=255)                                                             aaGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGt                                                                    >  2:426756/1‑140 (MQ=255)                                                               gCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTa                                                                  <  2:377104/140‑1 (MQ=255)                                                               gCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTa                                                                  >  2:275771/1‑140 (MQ=255)                                                                    aTCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCt                                                             >  1:692351/1‑140 (MQ=255)                                                                                 ttACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCttttttt                                                >  2:876097/1‑140 (MQ=255)                                                                                   aCTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTaa                                              <  2:67924/140‑1 (MQ=255)                                                                                          acgacCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAACCATTaa                                       >  1:46791/1‑140 (MQ=255)                                                                                          acgacCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTaa                                       >  2:171498/1‑140 (MQ=255)                                                                                          acgacCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTaa                                       >  1:879826/1‑140 (MQ=255)                                                                                              ccTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAAtt                                   >  2:265979/1‑140 (MQ=255)                                                                                                     gggCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGt                            <  1:876097/140‑1 (MQ=255)                                                                                                        cGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAAt                         >  1:442320/1‑140 (MQ=255)                                                                                                          gATTTTCTCCAGCTTCTGTATTGGTAAGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGt                            <  1:666461/140‑1 (MQ=255)                                                                                                          gATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAAttt                       >  2:38252/1‑140 (MQ=255)                                                                                                                     gCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAGGCCGCATTCTGACTGTCAGATGCTACTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCCTTTTTTAATCATTAAAATTCTTTCGTAATTTATAATCTTTaa            <  2:767447/140‑1 (MQ=255)                                                                                                                         cTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTaaaaaa        <  1:317545/140‑1 (MQ=255)                                                                                                                          tGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTaaaaaaa       <  1:240311/140‑1 (MQ=255)                                                                                                                          tGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTaaaaaaa       <  2:168024/140‑1 (MQ=255)                                                                                                                               t‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCAtt  <  2:713054/140‑1 (MQ=255)                                                                                                                               t‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCAtt  <  2:692351/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                           GTTGGGAGCCTGGGCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATT‑‑‑‑‑GGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATT  >  NC_000913/3888057‑3888322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.