Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	199,188	C→A	R421S (CGC→AGC)	bamA →	outer membrane protein assembly factor BamA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	199,188	0	C	A	100.0%	214.3 / NA	60	R421S (CGC→AGC)	bamA	outer membrane protein assembly factor BamA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (26/34);  total (26/34)

CATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGACCAAAAACGATTA  >  NC_000913/199051‑199321                                                                                                                                          |                                                                                                                                      cATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAgagc                                                                                                                                     >  1:136236/1‑140 (MQ=255) cATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAgagc                                                                                                                                     >  1:464805/1‑140 (MQ=255)    ggCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAac                                                                                                                                  >  2:568165/1‑140 (MQ=255)        ggggAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCg                                                                                                                              <  1:466470/140‑1 (MQ=255)         gggAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCgg                                                                                                                             >  1:907167/1‑140 (MQ=255)         gggAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCgg                                                                                                                             >  1:184821/1‑140 (MQ=255)         gggAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCgg                                                                                                                             <  2:412329/140‑1 (MQ=255)           gAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTa                                                                                                                           >  2:830589/1‑140 (MQ=255)               gATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCtt                                                                                                                       <  1:415629/140‑1 (MQ=255)                    ggTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACt                                                                                                                  <  1:373227/140‑1 (MQ=255)                     gTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACtt                                                                                                                 <  1:204987/140‑1 (MQ=255)                     gTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACtt                                                                                                                 <  1:62740/140‑1 (MQ=255)                          tCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTa                                                                                                            >  2:505663/1‑140 (MQ=255)                           cAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTAt                                                                                                           >  2:826877/1‑140 (MQ=255)                                 aaGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGTTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTa                                                                                                     <  2:634698/140‑1 (MQ=255)                                   ggAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACg                                                                                                   <  2:136236/140‑1 (MQ=255)                                     aGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGGCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAGCTTTGGTATTGGTTACGGt                                                                                                 <  2:167222/140‑1 (MQ=255)                                        gTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACt                                                                                              >  2:551781/1‑140 (MQ=255)                                        gTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACt                                                                                              >  2:712461/1‑140 (MQ=255)                                        gTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAAC‑TTGGTATTGGTTACGGTACTg                                                                                             >  1:903629/1‑140 (MQ=255)                                        gTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGAAGCTTCAACTTTGGTATTGGTTACGGTACt                                                                                              >  2:894649/1‑140 (MQ=255)                                        gTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTAC‑AGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTg                                                                                             >  1:317500/1‑140 (MQ=255)                                           tGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAAC‑TTGGTATTGGTTACGGTACTGaaa                                                                                          <  2:903629/140‑1 (MQ=255)                                               tCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTa                                                                                                     >  2:157158/1‑126 (MQ=255)                                               tCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTa                                                                                                     <  1:157158/126‑1 (MQ=255)                                                    tGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTg                                                                                  <  2:482002/140‑1 (MQ=255)                                                         ttcttTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCtt                                                                             <  1:239151/140‑1 (MQ=255)                                                              tGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAgg                                                                        <  2:629659/140‑1 (MQ=255)                                                              tGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAgg                                                                        <  1:77123/140‑1 (MQ=255)                                                                 aaCTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTg                                                                     <  1:712461/140‑1 (MQ=255)                                                                      tCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGtgtg                                                                >  1:701959/1‑140 (MQ=255)                                                                      tCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGtgtg                                                                >  2:202544/1‑140 (MQ=255)                                                                         ataccgataccCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTgcag                                                             <  1:830589/140‑1 (MQ=255)                                                                                taccCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATa                                                      >  1:802671/1‑140 (MQ=255)                                                                                 accCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATaa                                                     <  1:72493/140‑1 (MQ=255)                                                                                  ctcAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAAc                                                    <  2:459483/138‑1 (MQ=255)                                                                                  cccAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAAc                                                    <  1:505663/140‑1 (MQ=255)                                                                                  cccAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAAc                                                    <  1:40296/140‑1 (MQ=255)                                                                                  cccAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAAc                                                    <  2:701959/140‑1 (MQ=255)                                                                                  cccAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAAc                                                    <  2:607481/140‑1 (MQ=255)                                                                                    cAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGAt                                                       <  2:234614/135‑1 (MQ=255)                                                                                    cAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGAt                                                       >  1:234614/1‑135 (MQ=255)                                                                                    cAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTAC‑AGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTgg                                                 <  2:317500/140‑1 (MQ=255)                                                                                     aaCGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTgg                                                 <  2:907167/140‑1 (MQ=255)                                                                                      aCGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGt                                                <  1:260955/140‑1 (MQ=255)                                                                                      aCGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGt                                                <  1:894649/140‑1 (MQ=255)                                                                                      aCGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGt                                                <  1:551781/140‑1 (MQ=255)                                                                                       cGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGtt                                               <  2:464805/140‑1 (MQ=255)                                                                                        gtgtTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTa                                              <  1:568165/140‑1 (MQ=255)                                                                                           ttCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGt                                           >  2:652933/1‑140 (MQ=255)                                                                                            tCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTa                                          <  2:184821/140‑1 (MQ=255)                                                                                             ccGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGGAAAAGAGAGCAACACCGGCAGCTTCAACTTTGGTATTGGCTACGGTACTGAAAGTGGCGTGCGCTTCCACGCTGGTGTGCAGCAGGATAACTGGTTAGGAAc                                         >  2:551566/1‑140 (MQ=255)                                                                                                     cccGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATg                                 >  1:340982/1‑140 (MQ=255)                                                                                                          aCCAGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGtt                            >  2:833368/1‑140 (MQ=255)                                                                                                             aGGTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGt                         <  1:826877/140‑1 (MQ=255)                                                                                                              ggTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTa                        >  2:500051/1‑140 (MQ=255)                                                                                                               gTTGATGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTAt                       >  2:16549/1‑140 (MQ=255)                                                                                                                    tGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACg                  >  1:121922/1‑140 (MQ=255)                                                                                                                    tGTCGTCTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACg                  >  1:521957/1‑140 (MQ=255)                                                                                                                        gtcTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGAc              >  1:326690/1‑140 (MQ=255)                                                                                                                         tcTACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGAcc             >  2:506995/1‑140 (MQ=255)                                                                                                                           tACAAGGTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGACCaa           <  1:652933/140‑1 (MQ=255)                                                                                                                                ggTAAAAGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGTAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGACCAAAAACg      >  2:105590/1‑140 (MQ=255)                                                                                                                                    aaaGAGAGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGACCAAAAACGATTa  >  2:595162/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      CATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGACCAAAAACGATTA  >  NC_000913/199051‑199321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑