Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,881,732	C→A	A26A (GCG→GCT)	dmlR ←	DNA‑binding transcriptional regulator DmlR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,881,732	0	C	A	100.0%	131.2 / NA	38	A26A (GCG→GCT)	dmlR	DNA‑binding transcriptional regulator DmlR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (14/24);  total (14/24)

ATTCATAAATCCGCTCTCCTTCTTCGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGTCAATTA  >  NC_000913/1881599‑1881864                                                                                                                                      |                                                                                                                                     aTTCATAAATCCGCTCTCCTTCTTCGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCAGCCAcg                                                                                                                                <  1:715899/140‑1 (MQ=255)      tAAATCCGCTCTCCTTCTTCGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCAGCCACggcggc                                                                                                                           >  2:208389/1‑140 (MQ=255)             gCTCTCCTTCTTCGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCAGCCACGGCGGCAAAACCa                                                                                                                    >  1:85824/1‑140 (MQ=255)              ctctCCTTCTTCGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCAGCCACGGCGGCAAAACCAg                                                                                                                   >  1:894222/1‑140 (MQ=255)                        tcGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCAGCCACGGCGGCAAAACCAGCCCGGCGAGc                                                                                                         <  1:873333/140‑1 (MQ=255)                         cGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCAGCCACGGCGGCAAAACCAGCCCGGCGAGcc                                                                                                        <  1:862364/140‑1 (MQ=255)                           gTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCAGCCACGGCGGCAAAACCAGCCCGGCGAGccac                                                                                                      >  2:641733/1‑140 (MQ=255)                           gTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCAGCCACGGCGGCAAAACCAGCCCGGCGAGccac                                                                                                      <  1:584953/140‑1 (MQ=255)                           gTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCAGCCACGGCGGCAAAACCAGCCCGGCGAGccac                                                                                                      >  1:446647/1‑140 (MQ=255)                           gTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCAGCCACGGCGGCAAAACCAGCCCGGCGAGccac                                                                                                      <  1:338489/140‑1 (MQ=255)                                      gcggcgGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCAGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGa                                                                                           >  2:95581/1‑140 (MQ=255)                                         gcggGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCAGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGAcgc                                                                                        >  2:792437/1‑140 (MQ=255)                                            ggtggtGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCAGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCa                                                                                     <  2:149567/140‑1 (MQ=255)                                              tggtgCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCAGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCaaa                                                                                   <  1:36693/140‑1 (MQ=255)                                                      tgtAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCAGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTcag                                                                           <  1:65145/140‑1 (MQ=255)                                                      tgtAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCAGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTcag                                                                           <  2:577678/140‑1 (MQ=255)                                                      tgtAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCAGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTcag                                                                           <  2:46619/140‑1 (MQ=255)                                                      tgtAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCAGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTcag                                                                           <  2:196540/140‑1 (MQ=255)                                                       gtAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCAGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCa                                                                                     >  2:905066/1‑129 (MQ=255)                                                       gtAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCAGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCa                                                                                     <  1:905066/129‑1 (MQ=255)                                                           caacaCCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCAGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCg                                                                      <  1:22124/140‑1 (MQ=255)                                                                ccacGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCAGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTaaa                                                                 >  2:841546/1‑140 (MQ=255)                                                                ccacGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCAGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTaaa                                                                 >  1:214980/1‑140 (MQ=255)                                                                ccacGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCAGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTaaa                                                                 >  2:623179/1‑140 (MQ=255)                                                                  acGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCAGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAatt                                                               >  1:447358/1‑140 (MQ=255)                                                                  acGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCAGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAatt                                                               >  2:621994/1‑140 (MQ=255)                                                                      ttAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCAGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTc                                                           <  1:31514/140‑1 (MQ=255)                                                                        aGCGTTTGCTACAGCAAGGCGATGCGCTTGCTGAAGAACGCCGGTGAAACGCCCAGTTCTTCAGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCAt                                                         <  2:590162/140‑1 (MQ=255)                                                                             ttGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCAGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTc                                                    <  1:792437/140‑1 (MQ=255)                                                                                   cAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCAGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGttt                                              <  1:621994/140‑1 (MQ=255)                                                                                                  cTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCAGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGtt                               <  2:447358/140‑1 (MQ=255)                                                                                                    tGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCAGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTAc                             >  2:563586/1‑140 (MQ=255)                                                                                                              cGCCGGTGAAACGCCCAGTTCTTCAGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAAt                   <  1:208389/140‑1 (MQ=255)                                                                                                                     gAAACGCCCAGTTCTTCAGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCtttt            <  2:214980/140‑1 (MQ=255)                                                                                                                        ccGCCCAGTTCTTCAGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAg         <  2:114674/139‑1 (MQ=255)                                                                                                                         cGCCCAGTTCTTCAGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGt        >  1:742829/1‑140 (MQ=255)                                                                                                                         cGCCCAGTTCTTCAGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGt        <  2:446647/140‑1 (MQ=255)                                                                                                                         cGCCCAGTTCTTCAGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGt        <  2:894222/140‑1 (MQ=255)                                                                                                                         cGCCCAGTTCTTCAGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGt        <  2:320642/140‑1 (MQ=255)                                                                                                                            ccAGTTCTTCAGCGACGGAGGCAAAACCAGCCCGGCGAGACACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGTCaa     <  2:85824/140‑1 (MQ=255)                                                                                                                               gTTCTTCAGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGTCAATta  <  1:95581/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                     ATTCATAAATCCGCTCTCCTTCTTCGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGTCAATTA  >  NC_000913/1881599‑1881864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑