Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,374,861	C→A	G48V (GGG→GTG)	menE ←	o‑succinylbenzoate‑‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,374,861	0	C	A	100.0%	114.0 / NA	34	G48V (GGG→GTG)	menE	o‑succinylbenzoate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (18/16);  total (18/16)

CAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCCCCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAG  >  NC_000913/2374730‑2374995                                                                                                                                    |                                                                                                                                       cAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCACCTGCAcc                                                                                                                                <  1:302347/140‑1 (MQ=255)             gCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCACCTGCACCGCAAATCCGGAg                                                                                                                    <  1:476149/140‑1 (MQ=255)                              gcGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCACCTGCACCGCAAATCCGGAGGCTAATTCATCGAcgcg                                                                                                   <  2:211346/139‑1 (MQ=255)                              aCGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCACCTGCACCGCAAATCCGGAGGCTAATTCATCGAcgcg                                                                                                   >  2:419936/1‑140 (MQ=255)                              aCGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCACCTGCACCGCAAATCCGGAGGCTAATTCATCGAcgcg                                                                                                   <  1:251751/140‑1 (MQ=255)                              aCGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCACATGCACCGCAAATCCGGAGGCTAATTCATCGAcgcg                                                                                                   >  2:274250/1‑140 (MQ=255)                                      cacCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCACCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACaaa                                                                                           >  1:195439/1‑140 (MQ=255)                                            cgcCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCACCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCt                                                                                        <  2:621382/137‑1 (MQ=255)                                            cgcCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCACCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCt                                                                                        >  1:621382/1‑137 (MQ=255)                                            cgcCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCACCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTcgc                                                                                     <  1:501377/140‑1 (MQ=255)                                                 cGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCACCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCgcgc                                                                                   >  1:232823/1‑137 (MQ=255)                                                 cGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCACCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCgcgc                                                                                   <  2:232823/137‑1 (MQ=255)                                                 cGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCACCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCag                                                                                >  1:877/1‑140 (MQ=255)                                                   cATTGCAGTAACGCTAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCACCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCagtt                                                                              >  2:658171/1‑140 (MQ=255)                                                      tGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCACCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCagttgag                                                                           >  2:68696/1‑140 (MQ=255)                                                           tAACGCTAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCACCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCt                                                                      <  1:658171/140‑1 (MQ=255)                                                            aaCGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCACCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTc                                                                     <  1:819383/140‑1 (MQ=255)                                                             aCGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCACCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCg                                                                    >  2:209869/1‑140 (MQ=255)                                                                ccagccagGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCACCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCa                                                                 >  1:163462/1‑140 (MQ=255)                                                                ccagccagGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCACCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCa                                                                 >  1:689771/1‑140 (MQ=255)                                                                  agccagGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCACCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCAtt                                                               >  2:47656/1‑140 (MQ=255)                                                                             cagcGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCACCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAgg                                                    >  2:99051/1‑140 (MQ=255)                                                                             cagcGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCACCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAgg                                                    >  1:447795/1‑140 (MQ=255)                                                                                  tttGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCACCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATg                                               >  2:230579/1‑140 (MQ=255)                                                                                                     ccGCAACATCACGCCGCTGCCCTCAACCACCACCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTgcc                            >  2:269166/1‑140 (MQ=255)                                                                                                          aCATCACGCCGCTGCCCTCAACCACCACCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAg                       <  1:419936/140‑1 (MQ=255)                                                                                                           cATCACGCCGCTGCCCTCAACCACCACCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGt                      <  1:68696/140‑1 (MQ=255)                                                                                                               aCGCCGCTGCCCTCAACCACCACCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGCTGCTCGTCATTAAGACGTAAGGCGATGGCTTCCCCCCGCACTTGCCGCCAGTGACg                  >  2:865444/1‑140 (MQ=255)                                                                                                                cgccgcTGCCCTCAACCACCACCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGc                 <  1:53999/140‑1 (MQ=255)                                                                                                                  ccgcTGCCCTCAACCACCACCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCa               <  2:163462/140‑1 (MQ=255)                                                                                                                  ccgcTGCCCTCAACCACCACCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCa               <  2:689771/140‑1 (MQ=255)                                                                                                                     cTGCCCTCAACCACCACCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACgg            <  1:209869/140‑1 (MQ=255)                                                                                                                        cccTCAACCACCACCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCa         >  2:699196/1‑140 (MQ=255)                                                                                                                         ccTCAACCACCACCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCC‑CCAGTGACGCCACGGCCAGt       <  2:367058/140‑1 (MQ=255)                                                                                                                              accaccaccTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCaga   <  1:47656/140‑1 (MQ=255)                                                                                                                               ccaccaCCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCagag  <  1:274250/140‑1 (MQ=255)                                                                                                                               ccaccaCCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCagag  <  1:230579/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                       CAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCCCCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAG  >  NC_000913/2374730‑2374995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑