Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,945,626	G→T	Q73K (CAA→AAA)	tcdA ←	tRNA threonylcarbamoyladenosine dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,945,626	0	G	T	100.0%	202.2 / NA	56	Q73K (CAA→AAA)	tcdA	tRNA threonylcarbamoyladenosine dehydratase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (34/22);  total (34/22)

TATACTGCGCTACGTTATCCGGCGTCACGAAATCATCCACCACCGTTACACGGCACTCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTGCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGTGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAAC  >  NC_000913/2945491‑2945762                                                                                                                                        |                                                                                                                                         tataCTGCGCTACGTTATCCGGCGTCACGAAATCATCCACCACCGTTACACGGCACTCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGa                                                                                                                                      <  1:723623/140‑1 (MQ=255)  ataCTGCGCTACGTTATCCGGCGTCACGAAATCATCCACCACCGTTACACGGCACTCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGat                                                                                                                                     <  2:196816/140‑1 (MQ=255)      tGCGCTACGTTATCCGGCGTCACGAAATCATCCACCACCGTTACACGGCACTCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGattggt                                                                                                                                 >  2:404212/1‑140 (MQ=255)      tGCGCTACGTTATCCGGCGTCACGAAATCATCCACCACCGTTACACGGCACTCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGattggt                                                                                                                                 >  2:289274/1‑140 (MQ=255)      tGCGCTACGTTATCCGGCGTCACGAAATCATCCACCACCGTTACACGGCACTCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGattggt                                                                                                                                 >  1:486362/1‑140 (MQ=255)      tGCGCTACGTTATCCGGCGTCACGAAATCATCCACCACCGTTACACGGCACTCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGattggt                                                                                                                                 >  2:800718/1‑140 (MQ=255)        cgccaCGTTATCCGGCGTCACGAAATCATCCACCACCGTTACACGGCACTCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGattggtat                                                                                                                               >  2:275185/5‑140 (MQ=255)        cgcTACGTTATCCGGCGTCACGAAATCATCCACCACCGTTACACGGCACTCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGattggtat                                                                                                                               >  1:301130/1‑140 (MQ=255)        cgcTACGTTATCCGGCGTCACGAAATCATCCACCACCGTTACACGGCACTCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGattggtat                                                                                                                               >  1:816855/1‑140 (MQ=255)        cgcTACGTTATCCGGCGTCACGAAATCATCCACCACCGTTACACGGCACTCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGattggtat                                                                                                                               >  2:736299/1‑140 (MQ=255)        cgcTACGTTATCCGGCGTCACGAAATCATCCACCACCGTTACACGGCACTCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGattggtat                                                                                                                               >  1:568719/1‑140 (MQ=255)        cgcTACGTTATCCGGCGTCACGAAATCATCCACCACCGTTACACGGCACTCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGattggtat                                                                                                                               >  2:236002/1‑140 (MQ=255)        cgcTACGTTATCCGGCGTCACGAAATCATCCACCACCGTTACACGGCACTCCGGGTTAATCTAGCGAATACGCTCCGCAATACCTTCCGCTTCTGCCACCCCAACGGTATCGCGCAGGGCATGAATTTTCCGattggtat                                                                                                                               >  2:106799/1‑140 (MQ=255)        cgcTACGTTATCCGGCGTCACGAAATCATCCACCACCGTTACACGGCACCCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGattggtat                                                                                                                               >  2:363259/1‑140 (MQ=255)            aCGTTATCCGGCGTCACGAAATCATCCACCACCGTTACACGGCACTCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGattggtattggt                                                                                                                           <  1:275185/140‑1 (MQ=255)             cGTTATCCGGCGTCACGAAATCATCCACCACCGTTACACGGCACTCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTg                                                                                                                          <  1:800718/140‑1 (MQ=255)                tATCCGGCGTCACGAAATCATCCACCACCGTTACACGGCACCCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTGACg                                                                                                                       <  1:363259/140‑1 (MQ=255)                  tCCGGCGTCACGAAATCATCCCCCACCGTTCCACGGCACTCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTGACGca                                                                                                                     <  2:840450/140‑1 (MQ=255)                  tCCGGCGTCACGAAATCATCCACCACCGTTACACGGCACTCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTGACGca                                                                                                                     >  2:184457/1‑140 (MQ=255)                     ggtttcccGAAATCACCCCCCCCCGTTACGCGGCTCTCCGGTTTAATCTGGCGAATCCGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTGACGcacac                                                                                                                  <  2:568719/133‑1 (MQ=255)                      gCGTCACGAAATCATCCACCACCGTTACACGGCACTCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTGACGcacaca                                                                                                                 <  1:736299/140‑1 (MQ=255)                       cGTCACGAAATCATCCACCACCGTTACACGGCACTCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTGACGCACAcat                                                                                                                >  2:356645/1‑140 (MQ=255)                         tCACGAAATCATCCACCACCGTTACACGGCACTCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTGACGCACAcatca                                                                                                              >  2:568801/1‑140 (MQ=255)                         tCACGAAATCATCCACCACCGTTACACGGCACTCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTGACGCACAcatca                                                                                                              <  1:184457/140‑1 (MQ=255)                           aCGAAATCATCCACCACCGTTACACGGCACTCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTGACGCACACatcatc                                                                                                            >  2:625511/1‑140 (MQ=255)                              aaaTCATCCACCACCGTTACACGGCACTCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTGACGCACACATCATCCat                                                                                                         <  1:701398/140‑1 (MQ=255)                                atcatcCCCCACCGTTACACGGCACTCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTGACGCACACATCATCCatat                                                                                                       <  1:235173/140‑1 (MQ=255)                                    tcCACCACCGTTACACGGCACTCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTGACGCACACATCATCCATATCGAt                                                                                                   >  1:586769/1‑140 (MQ=255)                                       accaccGTTACACGGCACTCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAg                                                                                                >  1:396698/1‑140 (MQ=255)                                        ccaccGTTACACGGCACTCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGc                                                                                               >  1:552383/1‑140 (MQ=255)                                            cGTTACACGGCACTCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGTGa                                                                                           >  2:761220/1‑140 (MQ=255)                                                      cACTCCGGGTTAATCTGGCGAATACGCTCCGCCTTAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGATTGGTA‑TGGTGACGCACACATCATCCAATACGGTAAGCGTGATTGGGGCAATc                                                                                >  2:779774/1‑140 (MQ=255)                                                        cTCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGTGATTGCGCCAATcc                                                                               >  2:69921/1‑140 (MQ=255)                                                         tCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGTGATTGCGCCAATccc                                                                              >  2:903672/1‑140 (MQ=255)                                                             ggTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGTGATTGCGCCAATCCCCGTg                                                                          <  1:596034/140‑1 (MQ=255)                                                              gTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGTGATTGCGCCAATCCCCGTgc                                                                         >  1:429025/1‑140 (MQ=255)                                                                  aTCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTGACACACACATCATCCATATCGATAAGCGTGATTGCGCCAATCCCCGTgcgcgc                                                                     >  1:2297/1‑140 (MQ=255)                                                                   tCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGTGATTGCGCCAATCCCCGTGCGCGcc                                                                    <  1:625511/140‑1 (MQ=255)                                                                       gCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGTGATTGCGCCAATCCCCGTGCGCGCCAgcg                                                                >  1:879123/1‑140 (MQ=255)                                                                              cGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGTGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTccgc                                                         <  1:289274/140‑1 (MQ=255)                                                                                         aaCTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGTGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAAc                                              >  1:115361/1‑140 (MQ=255)                                                                                          aCTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTGACGCACCCATCATCCATATCGATAAGCGTGATTGCGCCAATCCCCGTGCGCCCCAGCGCTTCCGCCGCCCAGGAAcc                                             <  2:301130/140‑1 (MQ=255)                                                                                            ttCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGTGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGa                                           <  2:396698/140‑1 (MQ=255)                                                                                              ccGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGTGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGaca                                         >  2:148282/1‑140 (MQ=255)                                                                                              ccGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGTGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCACCGCCCAGGAACCGaca                                         <  2:486362/140‑1 (MQ=255)                                                                                               cGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGTGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGacac                                        >  1:636730/1‑140 (MQ=255)                                                                                                  ttttGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGTGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGAcaccac                                     <  1:732191/140‑1 (MQ=255)                                                                                                        cAGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGTGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATac                               <  1:478587/140‑1 (MQ=255)                                                                                                         aGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGTGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATacc                              <  2:865349/140‑1 (MQ=255)                                                                                                         aGCCCAACGTTATCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGTGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATacc                              <  2:21026/140‑1 (MQ=255)                                                                                                                    aTCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGTGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACaaa                   >  2:736978/1‑140 (MQ=255)                                                                                                                     tCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGTGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAat                  >  1:514954/1‑140 (MQ=255)                                                                                                                     tCGCGCAGGGCATGAATTTTCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGTGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAat                  >  2:537052/1‑140 (MQ=255)                                                                                                                      cgcgCAGGGCATGAATTTTCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGTGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAata                 >  1:746549/1‑140 (MQ=255)                                                                                                                          cAGGGCATGAATTTTCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGTGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATgcg             <  2:97804/140‑1 (MQ=255)                                                                                                                          cAGGGCATGAATTTTCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGTGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATgcg             >  2:444699/1‑140 (MQ=255)                                                                                                                             ggCATGAATTTTCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGTGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGt          >  2:482466/1‑140 (MQ=255)                                                                                                                             ggCATGAATTTTCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGTGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGt          >  2:240590/1‑140 (MQ=255)                                                                                                                                  gAATTTTCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGTGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCa     <  1:761220/140‑1 (MQ=255)                                                                                                                                    aTTTTCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGTGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCaaa   <  1:903672/140‑1 (MQ=255)                                                                                                                                     ttttCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGTGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAAc  <  1:404212/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         TATACTGCGCTACGTTATCCGGCGTCACGAAATCATCCACCACCGTTACACGGCACTCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTGCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGTGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAAC  >  NC_000913/2945491‑2945762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑