Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,952,852	G→T	P1051Q (CCG→CAG)	recB ←	exodeoxyribonuclease V subunit RecB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,952,852	0	G	T	100.0%	158.4 / NA	45	P1051Q (CCG→CAG)	recB	exodeoxyribonuclease V subunit RecB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (24/21);  total (24/21)

TTGGGTGTAAGCCGAACTGTCTTCACCCAACCAGTTGGATTTATAGTCGAGCAGGTAATAACGCCCTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCGGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAGCGGTTCACTAATCGGCAGATAAAACTCCATCTCCACCTGTTTATTGCGGGCGGAAAGTTGACTCAGGCTTACG  >  NC_000913/2952719‑2952986                                                                                                                                      |                                                                                                                                       ttGGGTGTAAGCCGAACTGTCTTCACCCAACCAGTTGGATTTATAGTCGAGCAGGTAATAACGCCCTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGc                                                                                                                                  >  2:844380/1‑140 (MQ=255) ttGGGTGTAAGCCGAACTGTCTTCACCCAACCAGTTGGATTTATAGTCGAGCAGGTAATAACGCCCTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGc                                                                                                                                  <  1:329891/140‑1 (MQ=255) ttGGGTGTAAGCCGAACTGTCTTCACCCAACCAGTTGGATTTATAGTCGAGCAGGTAATAACGCCCTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGc                                                                                                                                  >  1:818043/1‑140 (MQ=255)      tgtAAGCCGAACTGTCTTCACCCAACCAGTTGGATTTATAGTCGAGCAGGTAATAACGCCCTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCg                                                                                                                             >  1:104348/1‑140 (MQ=255)      tgtAAGCCGAACTGTCTTCACCCAACCAGTTGGATTTATAGTCGAGCAGGTAATAACGCCCTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCg                                                                                                                             >  1:797315/1‑140 (MQ=255)              gAACTGTCTTCACCCAACCAGTTGGATTTATAGTCGAGCAGGTAATAACGCCCTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACACACATGCATGAACTCCAGCGGCTCGCAGCCTGCGGATAGCgg                                                                                                                     >  2:337421/1‑140 (MQ=255)                      ttCACCCAACCAGTTGGATTTATAGTCGAGCAGGTAATAACGCCCTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACt                                                                                                             >  2:44989/1‑140 (MQ=255)                       tCACCCAACCAGTTGGATTTATAGTCGAGCAGGTAATAACGCCCTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTg                                                                                                            <  2:104348/140‑1 (MQ=255)                            cAACCAGTTGGATTTATAGTCGAGCAGGTAATAACGCCCTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGa                                                                                                       <  2:90534/140‑1 (MQ=255)                              aCCAGTTGGATTTATAGTCGAGCAGGTAATAACGCCCTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGAtt                                                                                                     >  2:435602/1‑140 (MQ=255)                              aCCAGTTGGATTTATAGTCGAGCAGGTAATAACGCCCTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGAtt                                                                                                     >  1:892488/1‑140 (MQ=255)                                    tGGATTTATAGTCGAGCAGGTAATAACGCCCTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTa                                                                                               <  2:640168/140‑1 (MQ=255)                                         ttATAGTCGAGCAGGTAATAACGCCCTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAg                                                                                          <  1:435602/140‑1 (MQ=255)                                             aGTCGAGCAGGTAATAACGCCCTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTTTCAAGCTGa                                                                                      >  2:418667/1‑140 (MQ=255)                                             aGTCGAGCAGGTAATAACGCCCTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGa                                                                                      <  1:122025/140‑1 (MQ=255)                                             aGTCGAGCAGGTAATAACGCCCTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGa                                                                                      <  1:314108/140‑1 (MQ=255)                                                cGAGCAGGTAATAACGCCCTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGACTg                                                                                   >  2:733851/1‑140 (MQ=255)                                                 gAGCAGGTAATAACGCCCTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGACTgg                                                                                  >  1:803923/1‑140 (MQ=255)                                                 gAGCAGGTAATAACGCCCTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGACTgg                                                                                  >  1:483844/1‑140 (MQ=255)                                                 gAGCAGGTAATAACGCCCTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGACTgg                                                                                  >  1:418572/1‑140 (MQ=255)                                                   gCAGGTAATAACGCCCTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCg                                                                                <  1:813597/140‑1 (MQ=255)                                                        taataaCGCCCTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAg                                                                           <  1:309998/140‑1 (MQ=255)                                                               gCCCTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAGCGGTTCa                                                                    <  2:889278/140‑1 (MQ=255)                                                                  cTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAGCGGTTCACTa                                                                 >  2:218073/1‑140 (MQ=255)                                                                    tCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAGCGGTTCACTAAt                                                               <  2:418572/140‑1 (MQ=255)                                                                        ggCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAGCGGTTCACTAATCGGc                                                           <  1:5842/140‑1 (MQ=255)                                                                        ggCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAGCGGTTCACTAATCGGc                                                           <  2:803923/140‑1 (MQ=255)                                                                        ggCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAGCGGTTCACTAATCGGc                                                           <  1:97519/140‑1 (MQ=255)                                                                         gCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAGCGGTTCACTAATCGGCa                                                          >  2:598062/1‑140 (MQ=255)                                                                                       cGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAGCGGTTCACTAATCGGCAGATAAAACTCCAtc                                            <  2:818043/140‑1 (MQ=255)                                                                                        gATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAGCGGTTCACTAATCGGCAGATAAAACTCCAtct                                           <  2:797315/140‑1 (MQ=255)                                                                                         aTAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAGCGGTTCACTAATCGGCAGATAAAACTCCAtctc                                          <  2:160709/140‑1 (MQ=255)                                                                                         aTAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAGCGGTTCACTAATCGGCAGATAAAACTCCAtctc                                          >  1:761769/1‑140 (MQ=255)                                                                                         aTAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAGCGGTTCACTAATCGGCAGATAAAACTCCAtctc                                          >  1:485226/1‑140 (MQ=255)                                                                                         aTAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAGCGGTTCACTAATCGGCAGATAAAACTCCAtctc                                          >  2:484167/1‑140 (MQ=255)                                                                                         aTAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAGCGGTTCACTAATCGGCAGATAAAACTCCAtctc                                          <  1:844380/140‑1 (MQ=255)                                                                                          tAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAGCGGTTCACTAATCGGCAGATAAAACTCCATCTcc                                         >  1:902306/1‑140 (MQ=255)                                                                                            aaGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAGCGGTTCACTAATCGGCAGATAAAACTCCATCTCCAc                                       <  2:892488/140‑1 (MQ=255)                                                                                            aaGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAGCGGTTCACTAATCGGCAGATAAAACTCCATCTCCAc                                       <  2:641686/140‑1 (MQ=255)                                                                                                   ttAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAGCGGTTCACTAATCGGCAGATAAAACTCCATCTCCACCTGTTTa                                >  1:460423/1‑140 (MQ=255)                                                                                                    tAACATGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAGCGGTTCACTAATCGGCAGATAAAACTCCATCTCCACCTGTTTAt                               >  2:410663/1‑140 (MQ=255)                                                                                                         tGCCACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAGCGGTTCACTAATCGGCAGATAAAACTCCATCTCCACCTGTTTATTgcgg                          >  2:534654/1‑140 (MQ=255)                                                                                                            cACGTACCTGCATGAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAGCGGTTCACTAATCGGCAGATAAAACTCCATCTCCACCTGTTTATTgcgggcg                       <  1:315310/140‑1 (MQ=255)                                                                                                                         gAACTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAGCGGTTCACTAATCGGCAGATAAAACTCCATCTCCACCTGTTTATTGCGGGCGGAAAGTTGACTCa          >  2:883805/1‑140 (MQ=255)                                                                                                                          aaCTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAGCGGTTCACTAATCGGCAGATAAAACTCCATCTCCACCTGTTTATTGCGGGCGGAAAGTTGACTCAg         >  1:357137/1‑140 (MQ=255)                                                                                                                           aCTCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAGCGGTTCACTAATCGGCAGATAAAACTCCATCTCCACCTGTTTATTGCGGGCGGAAAGTTGACTCAgg        >  1:135031/1‑140 (MQ=255)                                                                                                                             tCCAGCGGCTGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAGCGGTTCACTAATCGGCAGATAAAACTCCATCTCCACCTGTTTATTGCGGGCGGAAAGTTGACTCAGGCt      >  1:25370/1‑140 (MQ=255)                                                                                                                                 gcggctGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAGCGGTTCACTAATCGGCAGATAAAACTCCATCTCCACCTGTTTATTGCGGGCGGAAAGTTGACTCAGGCTTACg  >  1:881734/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                       TTGGGTGTAAGCCGAACTGTCTTCACCCAACCAGTTGGATTTATAGTCGAGCAGGTAATAACGCCCTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCGGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAGCGGTTCACTAATCGGCAGATAAAACTCCATCTCCACCTGTTTATTGCGGGCGGAAAGTTGACTCAGGCTTACG  >  NC_000913/2952719‑2952986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑