Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,443,534	C→A	L385M (CTG→ATG)	tamA →	translocation and assembly module subunit TamA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,443,534	0	C	A	100.0%	207.3 / NA	58	L385M (CTG→ATG)	tamA	translocation and assembly module subunit TamA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (38/20);  total (38/20)

AACGATACCGAATCTGACTCCACTACGCTGGTGGCTTCTCGCTACTGGGATCTCTCCAGCGGCTGGCAGCGTGCCATTAACCTGCGCTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGCTGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCGCAACGCTACTCTATCGACTACTCCAACACGGCCTGGGGTTCAGATGTCGATTTCTCCGTTTTCC  >  NC_000913/4443399‑4443669                                                                                                                                        |                                                                                                                                        aaCGATACCGAATCTGACTCCACTACGCTGGTGGCTTCTCGCTACTGGGATCTCTCCAGCGGCTGGCAGCGTGCCATTAACCTGCGCTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGtt                                                                                                                                     <  2:230075/140‑1 (MQ=255) aaCGATACCGAATCTGACTCCACTACGCTGGTGGCTTCTCGCTACTGGGATCTCTCCAGCGGCTGGCAGCGTGCCATTAACCTGCGCTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGtt                                                                                                                                     <  1:413455/140‑1 (MQ=255) aaCGATACCGAATCTGACTCCACTACGCTGGTGGCTTCTCGCTACTGGGATCTCTCCAGCGGCTGGCAGCGTGCCATTAACCTGCGCTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGtt                                                                                                                                     <  1:403565/140‑1 (MQ=255)      tACCGAATCTGACTCCACTACGCTGGTGGCTTCTCGCTACTGGGATCTCTCCAGCGGCTGGCAGCGTGCCATTAACCTGCGCTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATc                                                                                                                                >  1:876255/1‑140 (MQ=255)      tACCGAATCTGACTCCACTACGCTGGTGGCTTCTCGCTACTGGGATCTCTCCAGCGGCTGGCAGCGTGCCATTAACCTGCGCTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATc                                                                                                                                <  2:362029/140‑1 (MQ=255)         cGAATCTGACTCCACTACGCTGGTGGCTTCTCGCTACTGGGATCTCTCCAGCGGCTGGCAGCGTGCCATTAACCTGCGCTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTg                                                                                                                             <  2:876255/140‑1 (MQ=255)                  cTCCACTACGCTGGTGGCTTCTCGCTACTGGGATCTCTCCAGCGGCTGGCAGCGTGCCATTAACCTGCGCTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGtgatga                                                                                                                    <  2:329969/140‑1 (MQ=255)                             tggtggCTCCTCGCTACTGGGATCTCTCCAGCGGCTGGCAGCGTGCCATTAACCTGCGCTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCAcg                                                                                                         >  1:888009/1‑140 (MQ=255)                             tcggggCTTGTCGCTACTGGGATATATCCAACCGCTTGCAGCGTGCCGTTAACCTGCGCTGGCGTCATGATCACTTTACCCAGTGTGAAATTACCACAACCACGATGAGGTTTTATCCTGGGGTGATGATTAGCcgcgcg                                                                                                         <  2:210492/136‑1 (MQ=255)                               gtggCTTCTCGCTACTGGGATCTCTCCAGCGGCTGGCAGCGTGCCATTAACCTGCGCTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCAcgcg                                                                                                       >  1:30588/1‑140 (MQ=255)                               gtggCTTCTCGCTACTGGGATCTCTCCAGCGGCTGGCAGCGTGCCATTAACCTGCGCTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCAcgcg                                                                                                       >  1:341308/1‑140 (MQ=255)                               gtggCTTCTCGCTACTGGGATCTCTCCAGCGGCTGGCAGCGTGCCATTAACCTGCGCTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCAcgcg                                                                                                       >  1:700347/1‑140 (MQ=255)                                  gCTTCTCGCTACTGGGATCTCTCCAGCGGCTGGCAGCGTGCCATTAACCTGCGCTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTtc                                                                                                    <  1:365701/140‑1 (MQ=255)                                  gCTTCTCGCTACTGGGATCTCTCCAGCGGCTGGCAGCGTGCCATTAACCTGCGCTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTtc                                                                                                    <  2:700347/140‑1 (MQ=255)                                  gCTTCTCGCTACTGGGATCTCTCCAGCGGCTGGCAGCGTGCCATTAACCTGCGCTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTtc                                                                                                    <  2:341308/140‑1 (MQ=255)                                             cTGGGATCTCTCCAGCGGCTGGCAGCGTGCCATTAACCTGCGCTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGcc                                                                                         <  2:888009/140‑1 (MQ=255)                                                 gATCTCTCCAGCGGCTGGCAGCGTGCCATTAACCTGCGCTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGAt                                                                                     >  1:474551/1‑140 (MQ=255)                                                     tctcCAGCGGCTGGCAGCGTGCCATTAACCTGCGCTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCa                                                                                 >  2:181377/1‑140 (MQ=255)                                                     tctcCAGCGGCTGGCAGCGTGCCATTAACCTGCGCTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCa                                                                                 >  2:519901/1‑140 (MQ=255)                                                               cTGGCAGCGTGCCATTAACCTGCGCTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCg                                                                       >  2:9812/1‑140 (MQ=255)                                                               cTGGCAGCGTGCCATTAACCTGCGCTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCg                                                                       >  2:108093/1‑140 (MQ=255)                                                                 ggCAGCGTGCCATTAACCTGCGCTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGAc                                                                     >  2:557195/1‑140 (MQ=255)                                                                 ggCAGCGTGCCATTAACCTGCGCTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGAc                                                                     >  2:415896/1‑140 (MQ=255)                                                                 ggCAGCGTGCCATTAACCTGCGCTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGAc                                                                     >  2:105459/1‑140 (MQ=255)                                                                 ggCAGCGTGCCATTAACCTGCGCTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGAc                                                                     >  2:174701/1‑140 (MQ=255)                                                                    aGCGTGCCATTAACCTGCGCTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCg                                                                  <  1:710826/140‑1 (MQ=255)                                                                       gTGCCATTAACCTGCGCTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCGCaa                                                               <  1:9812/140‑1 (MQ=255)                                                                        tGCCATTAACCTGCGCTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCGCAAc                                                              >  1:546074/1‑140 (MQ=255)                                                                         gCCATTAACCTGCGCTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCGCAACg                                                             <  1:260381/140‑1 (MQ=255)                                                                             ttAACCTGCGCTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCGCAACGCTAc                                                         >  2:746820/1‑140 (MQ=255)                                                                              tAACCTGCGCTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCGCAACGCTAct                                                        <  1:174701/140‑1 (MQ=255)                                                                                 ccTGCGCTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCGCAACGCTACTCTa                                                     <  1:746820/140‑1 (MQ=255)                                                                                   tGCGCTGGAGTCTCGACGACTTTACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCGCAACGCTACTCTATc                                                   >  2:504017/1‑140 (MQ=255)                                                                                     cgcTGGAGTCTCGACCACTTTACTCTGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCGCAACGCTACTCTATCGa                                                 >  2:754143/1‑140 (MQ=255)                                                                                     cgcTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATGCTGGGGCGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCGCAACGCTACTCTATCGa                                                 >  2:92758/1‑140 (MQ=255)                                                                                     cgcTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCGCAACGCTACTCTATCGa                                                 >  1:274393/1‑140 (MQ=255)                                                                                     cgcTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCGCAACGCTACTCTATCGa                                                 >  2:686941/1‑140 (MQ=255)                                                                                      gcTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCGCAACGCTACTCTATCGac                                                <  1:686941/140‑1 (MQ=255)                                                                                       cTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCGCAACGCTACTCTATCGact                                               >  2:422746/1‑140 (MQ=255)                                                                                       cTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCGCAACGCTACTCTATCGact                                               >  1:365211/1‑140 (MQ=255)                                                                                       cTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCGCAACGCTACTCTATCGact                                               >  2:37420/1‑140 (MQ=255)                                                                                                       tttACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCGCAACGCTACTCTATCGACTACTCCAACACGGCCTg                               <  2:228244/140‑1 (MQ=255)                                                                                                        ttgcTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCGCAACGCTACTCTATCGACTACTCCAACACGGCCTgg                              <  2:829471/137‑1 (MQ=255)                                                                                                         tACTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCGCAACGCTACTCTATCGACTACTCCAACACGGCCTggg                             >  1:668177/1‑140 (MQ=255)                                                                                                           cTCAGGGTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCGCAACGCTACTCTATCGACTACTCCAACACGGCCTGGGGt                           >  2:778567/1‑140 (MQ=255)                                                                                                               gggTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCGCAACGCTACTCTATCGACTACTCCAACACGGCCTGGGGTTCAg                       >  2:761263/1‑140 (MQ=255)                                                                                                                ggTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCGCAACGCTACTCTATCGACTACTCCAACACGGCCTGGGGTTCAGa                      >  1:427693/1‑140 (MQ=255)                                                                                                                 gggAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCGCAACGCTACTCTATCGACTACTCCAACACGGCCTGGGGTTCAGAt                     >  1:150139/3‑140 (MQ=255)                                                                                                                 gTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCGCAACGCTACTCTATCGACTACTCCAACACGGCCTGGGGTTCAGAt                     >  1:474411/1‑140 (MQ=255)                                                                                                                 gTGAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCGCAACGCTACTCTATCGACTACTCCAACACGGCCTGGGGTTCAGAt                     >  2:94480/1‑140 (MQ=255)                                                                                                                   gAAATTACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCGCAACGCTACTCTATCGACTACTCCAACACGGCCTGGGGTTCAGATGt                   >  2:625966/1‑140 (MQ=255)                                                                                                                        tACCAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCGCAACGCTACTCTATCGACTACTCCAACACGGCCTGGGGTTCAGATGTCGAtt              <  2:534141/140‑1 (MQ=255)                                                                                                                          ccAATACCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCGCAACGCTACTCTATCGACTACTCCAACACGGCCTGGGGTTCAGATGTCGATTtc            >  1:353831/1‑140 (MQ=255)                                                                                                                              tACCACGATGATGTTTTATCCTGGGGCGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCGCAACGCTACTCTATCGACTACTCCAACACGGCCTGGGGTTCAGATGTCGATTTCTCCg        >  2:873848/1‑140 (MQ=255)                                                                                                                               aCCACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCGCAACGCTACTCTATCGACTACTCCAACACGGCCTGGGGTTCAGATGTCGATTTCTCCGt       <  1:754143/140‑1 (MQ=255)                                                                                                                                 cACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCGCAACGCTACTCTATCGACTACTCCAACACGGCCTGGGGTTCAGATGTCGATTTCTCCGttt     >  1:239491/1‑140 (MQ=255)                                                                                                                                 cACGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCGCAACGCTACTCTATCGACTACTCCAACACGGCCTGGGGTTCAGATGTCGATTTCTCCGttt     >  2:271629/1‑140 (MQ=255)                                                                                                                                  aCGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCGCAACGCTACTCTATCGACTACTCCAACACGGCCTGGGGTTCAGATGTCGATTTCTCCGtttt    >  1:292027/1‑140 (MQ=255)                                                                                                                                   cGATGATGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCGCAACGCTACTCTATCGACTACTCCAACACGGCCTGGGGTTCAGATGTCGATTTCTCCGTTTTc   >  1:722230/1‑140 (MQ=255)                                                                                                                                    gatgatGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCGCAACGCTACTCTATCGACTACTCCAACACGGCCTGGGGTTCAGATGTCGATTTCTCCGTTTTcc  >  1:533897/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        AACGATACCGAATCTGACTCCACTACGCTGGTGGCTTCTCGCTACTGGGATCTCTCCAGCGGCTGGCAGCGTGCCATTAACCTGCGCTGGAGTCTCGACCACTTTACTCAGGGTGAAATTACCAATACCACGATGCTGTTTTATCCTGGGGTGATGATTAGCCGCACGCGTTCTCGTGGTGGCCTGATGCCAACCTGGGGCGACTCGCAACGCTACTCTATCGACTACTCCAACACGGCCTGGGGTTCAGATGTCGATTTCTCCGTTTTCC  >  NC_000913/4443399‑4443669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑