Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	339,025	C→A	A234E (GCG→GAG)	yahG →	DUF1116 domain‑containing protein YahG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	339,025	0	C	A	100.0%	172.9 / NA	49	A234E (GCG→GAG)	yahG	DUF1116 domain‑containing protein YahG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (29/20);  total (29/20)

GCGTATGGGCGCTAACGACCAGAGCGTGATTGACCGCCTGAACTGGATGCGTGATGTGCAGGGACCAATACTGCGCGACGCGATGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGCGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACACTGCTGATTCAGGCGCTGACGCCGGGGATTATTCAGGCGGGTTATTCCGTCGAGCAACAGCGCGAAGTGTTTGAGTTTGTCGCCAG  >  NC_000913/338897‑339160                                                                                                                                 |                                                                                                                                        gCGTATGGGCGCTAACGACCAGAGCGTGATTGACCGCCTGAACTGGATGCGTGATGTGCAGGGACCAATACTGCGCGACGCGATGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATgg                                                                                                                              <  1:893157/140‑1 (MQ=255)      tGGGCGCTAACGACCAGAGCGTGATTGACCGCCTGAACTGGATGCGTGATGTGCAGGGACCAATACTGCGCGACGCGATGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGAt                                                                                                                         >  2:603776/1‑140 (MQ=255)       gggCGCTAAGGGCCAGAGCGTGATTGAGCGCCTGAACTGGATGCGTGATGTGCAGGGACCAATACTGCGCGACGCGATGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATg                                                                                                                        <  2:119437/140‑1 (MQ=255)                  aCCAGAGCGTGATTGACCGCCTGAACTGGATGCGTGATGTGCAGGGACCAATACTGCGCGACGCGATGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAAc                                                                                                             >  1:434732/1‑140 (MQ=255)                  aCCAGAGCGTGATTGACCGCCTGAACTGGATGCGTGATGTGCAGGGACCAATACTGCGCGACGCGATGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAAc                                                                                                             >  1:563952/1‑140 (MQ=255)                   ccAGAGCGTGATTGACCGCCTGAACTGGATGCGTGATGTGCAGGGACCAATACTGCGCGACGCGATGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAAcc                                                                                                            >  2:375126/1‑140 (MQ=255)                       agCGTGATTGACCGCCTGAACTGGATGCGTGATGTGCAGGGACCAATACTGCGCGACGTGATGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCaa                                                                                                        >  1:594527/1‑140 (MQ=255)                       agCGTGATTGACCGCCTGAACTGGATGCGTGATGTGCAGGGACCAATACTGCGCGACGCGATGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCaa                                                                                                        >  2:759653/1‑140 (MQ=255)                       agCGTGATTGACCGCCTGAACTGGATGCGTGATGTGCAGGGACCAATACTGCGCGACGCGATGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCaa                                                                                                        >  2:227194/1‑140 (MQ=255)                       agCGTGATTGACCGCCTGAACTGGATGCGTGATGTGCAGGGACCAATACTGCGCGACGCGATGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCaa                                                                                                        >  1:402511/1‑140 (MQ=255)                             aTTGACCGCCTGAACTGGATGCGTGATGTGCAGGGACCAATACTGCGCGACGCGATGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGc                                                                                                  <  2:80266/140‑1 (MQ=255)                             aTTGACCGCCTGAACTGGATGCGTGATGTGCAGGGACCAATACTGCGCGACGCGATGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGc                                                                                                  >  2:721363/1‑140 (MQ=255)                                     ccTGAACTGGATGCGTGATGTGCAGGGACCAATACTGCGCGACGCGATGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGgacga                                                                                          <  2:443664/140‑1 (MQ=255)                                       tGAACTGGATGCGTGATGTGCAGGGACCAATACTGCGCGACGCGATGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGaca                                                                                        <  2:563952/140‑1 (MQ=255)                                         aaCTGGATGCGTGATGTGCAGGGACCAATACTGCGCGACGCGATGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACAct                                                                                      <  1:721363/140‑1 (MQ=255)                                                    tGATGTGCAGGGACCAATACTGCGCGACGCGATGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACACTGCTGATTCAgg                                                                           >  2:606168/1‑140 (MQ=255)                                                      aTGTGCAGGGACCAATACTGCGCGACGCGATGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACACTGCTGATTCAGgcg                                                                         >  2:330521/1‑140 (MQ=255)                                                         tgCAGGGACCAATACTGCGCGACGCGATGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACACTGCTGATTCAGGCGCTg                                                                      <  2:499681/140‑1 (MQ=255)                                                              ggACCAATACTGCGCGACGCGATGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACACTGCTGATTCAGGCGCTGACGcc                                                                 <  1:603776/140‑1 (MQ=255)                                                                aCCAATACTGCGCGACGCGATGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACACTGCTGATTCAGGCGCTGACGCCgg                                                               >  2:575743/1‑140 (MQ=255)                                                                   aaTACTGCGCGACGTGATGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACACTGCTGATTCAGGCGCTGACGCCGGGGa                                                            <  2:594527/140‑1 (MQ=255)                                                                   aaTACTGCGCGACGCGATGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACACTGCTGATTCAGGCGCTg                                                                      >  2:502852/1‑130 (MQ=255)                                                                   aaTACTGCGCGACGCGATGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACACTGCTGATTCAGGCGCTg                                                                      <  1:502852/130‑1 (MQ=255)                                                                   aaTACTGCGCGACGCGATGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACACTGCTGATTCAGGCGCTGACGCCGGGGa                                                            >  2:223175/1‑140 (MQ=255)                                                                   aaTACTGCGCGACGCGATGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACACTGCTGATTCAGGCGCTGACGCCGGGGa                                                            <  2:402511/140‑1 (MQ=255)                                                                        tGCGCGACGCGATGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACACTGCTGATTCAGGCGCTGACGCCGGGGattatt                                                       >  2:135174/1‑140 (MQ=255)                                                                          cgcgacgcgaTGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACACTGCTGATTCAGGCGCt                                                                       <  2:268929/122‑1 (MQ=255)                                                                          cgcgacgcgaTGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACACTGCTGATTCAGGCGCt                                                                       >  1:268929/1‑122 (MQ=255)                                                                          cgcgacgcgaTGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACACTGCTGATTCAGGCGCTGACGCCGGGGATTATTCa                                                     >  1:504582/1‑140 (MQ=255)                                                                          cgcgacgcgaTGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACACTGCTGATTCAGGCGCTGACGCCGGGGATTATTCa                                                     >  2:170819/1‑140 (MQ=255)                                                                          cgcgacgcgaTGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACACTGCTGATTCAGGCGCTGACGCCGGGGATTATTCa                                                     >  2:627546/1‑140 (MQ=255)                                                                          cgcgacgcgaTGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACACTGCTGATTCAGGCGCTGACGCCGGGGATTATTCa                                                     >  2:361786/1‑140 (MQ=255)                                                                          cgcgacgcgaTGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACACTGCTGATTCAGGCGCTGACGCCGGGGATTATTCa                                                     >  1:172796/1‑140 (MQ=255)                                                                          cgcgacgcgaTGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACACTGCTGATTCAGGCGCTGACGCCGGGGATTATTCa                                                     >  2:423962/1‑140 (MQ=255)                                                                          cgcgacgcgaTGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACACTGCTGATTCAGGCGCTGACGCCGGGGATTATTCa                                                     >  2:544264/1‑140 (MQ=255)                                                                          cgcgacgcgaTGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCCGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACACTGCTGATTCAGGCGCTGACGCCGGGGATTATTCa                                                     >  1:127998/1‑140 (MQ=255)                                                                          cgcgacacgaTGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACACTGCTGATTCAGGCGCTGACGCCGGGGATTATTCa                                                     >  1:692686/1‑140 (MQ=255)                                                                           gcgacgcgaTGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACACTGCTGATTCAGGCGCTGACGCCGGGGATTATTCAg                                                    <  1:135174/140‑1 (MQ=255)                                                                            cgacgcgaTGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACACTGCTGATTCAGGCGCTGACGCCGGGGATTATTCAgg                                                   >  1:307288/1‑140 (MQ=255)                                                                             gacgcgaTGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACACTGCTGTTTCAGGCGCTGACGCCGGGGATTATTCAGGc                                                  >  1:390187/1‑140 (MQ=255)                                                                                                     aTCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACACTGCTGATTCAGGCGCTGACGCCGGGGATTATTCAGGCGGGTTATTCCGTCGAGCAACAgcg                          <  1:584434/140‑1 (MQ=255)                                                                                                     aTCGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACACTGCTGATTCAGGCGCTGACGCCGGGGATTATTCAGGCGGGTTATTCCGTCGAGCAACAgcg                          <  1:575743/140‑1 (MQ=255)                                                                                                       cGATCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACACTGCTGATTCAGGCGCTGACGCCGGGGATTATTCAGGCGGGTTATTCCGTCGAGCAACAGcgcg                        <  1:330521/140‑1 (MQ=255)                                                                                                        gATCTGCGCTTAATGCCGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACACTGCTGATTCAGGCGCTGACGCCGGGGATTATTCAGGCGGGTTATTCCGTCGAGCAACAGCGCGa                       <  2:127998/140‑1 (MQ=255)                                                                                                         aTCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACACTGCTGATTCAGGCGCTGACGCCGGGGATTATTCAGGCGGGTTATTCCGTCGAGCAACAGCGCGaa                      <  1:66043/140‑1 (MQ=255)                                                                                                         aTCTGCGCTTAATGCTGGCGCAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACACTGCTGATTCAGGCGCTGACGCCGGGGATTATTCAGGCGGGTTATTCCGTCGAGCAACAGCGCGaa                      <  1:375126/140‑1 (MQ=255)                                                                                                                           cgcAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACACTGCTGATTCAGGCGCTGACGCCGGGGATTATTCAGGCGGGTTATTCCGTCGAGCAACAGCGCGAAGTGTTTGAGTTTGTCGcc    >  2:495752/1‑140 (MQ=255)                                                                                                                           cgcAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACACTGCTGATTCAGGCGCTGACGCCGGGGATTATTCAGGCGGGTTATTCCGTCGAGCAACAGCGCGAAGTGTTTGAGTTTGTCGcc    >  2:851245/1‑140 (MQ=255)                                                                                                                             cAGGAGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACACTGCTGATTCAGGCGCTGACGCCGGGGATTATTCAGGCGGGTTATTCCGTCGAGCAACAGCGCGAAGTGTTTGAGTTTGTCGCcag  <  1:237928/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                        GCGTATGGGCGCTAACGACCAGAGCGTGATTGACCGCCTGAACTGGATGCGTGATGTGCAGGGACCAATACTGCGCGACGCGATGAAAATTATCGGCGAAATCGATCTGCGCTTAATGCTGGCGCAGGCGCTGCATATGGGCGATGAGTGCCATAACCGCAATAACGCCGGGACGACACTGCTGATTCAGGCGCTGACGCCGGGGATTATTCAGGCGGGTTATTCCGTCGAGCAACAGCGCGAAGTGTTTGAGTTTGTCGCCAG  >  NC_000913/338897‑339160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑