Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,498,390	C→A	P512Q (CCG→CAG)	opgD →	glucan biosynthesis protein D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,498,390	0	C	A	100.0%	160.7 / NA	47	P512Q (CCG→CAG)	opgD	glucan biosynthesis protein D
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (25/22);  total (25/22)

GCCGCGCCAAAAGGCATTGAGCCGGTGATTACGCTTTCCAGTGGGGAAGCGAAGCAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTT  >  NC_000913/1498254‑1498518                                                                                                                                         |                                                                                                                                 gCCGCGCCAAAAGGCATTGAGCCGGTGATTACGCTTTCCAGTGGGGAAGCGAAGCAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGt                                                                                                                               <  1:155429/140‑1 (MQ=255)  ccGCGCCAAAAGGCATTGAGCCGGTGATTACGCTTTCCAGTGGGGAAGCGAAGCAAATCGAAATTCTCTAAATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGTc                                                                                                                              >  2:54648/1‑140 (MQ=255)     cgcCAAAAGGCATTGAGCCGGAGATTACGCTTTCCAGTGGGGAAGCGCAGCAAATCGAAATTCTGTATATCGAACCCATCGTTGGATATCGTATCCAGTTTAACTGGTATCTGACTTCCGACTCCTCTGATCAGGTCGat                                                                                                                           >  2:286885/1‑140 (MQ=255)       ccAAAAGGCATTGAGCCGGTGATTACGCTTTCCAGTGGGGAAGCGAAGCAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGCCGatat                                                                                                                         <  2:886131/140‑1 (MQ=255)        cAAAAGGCATTGAGCCGGTGATTACGCTTTCCAGTGGGGAAGCGAAGCAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATg                                                                                                                        <  2:209168/140‑1 (MQ=255)           aaGGCATTGAGCCGGTGATTACGCTTTCCAGTGGGGAAGCGAAGCAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCgg                                                                                                                     <  1:860560/140‑1 (MQ=255)           aaGGCATTGAGCCGGTGATTACGCTTTCCAGTGGGGAAGCGAAGCAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCgg                                                                                                                     <  2:241526/140‑1 (MQ=255)           aaGGCATTGAGCCGGTGATTACGCTTTCCAGTGGGGAAGCGAAGCAAATCGAAATTCTCTATAGTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCgg                                                                                                                     <  2:510968/140‑1 (MQ=255)            aGGCATTGAGCCGGTGATTACGCTTTCCAGTGGGGAAGCGAAGCAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCGGa                                                                                                                    >  1:629889/1‑140 (MQ=255)             ggCATTGAGCCGGTGATTACGCTTTCCAGTGGGGAAGCGAAGCAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCGGAt                                                                                                                   <  1:699688/140‑1 (MQ=255)               cATTGAGCCGGTGATTACGCTTTCCAGTGGGGACCCGCAGCATATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCGGATGt                                                                                                                 >  2:420915/1‑140 (MQ=255)                  tGAGCCGGTGATTACGCTTTCCAGTGGGGAAGCGAAGCAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCATTGATCAGGTCGATATGCGGATGTATc                                                                                                              >  1:196225/1‑140 (MQ=255)                      ccGGTGATTACGCTTTCCAGTGGGGAAGCGAAGCAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCGGATGTATCTACg                                                                                                          <  2:736391/140‑1 (MQ=255)                        ggTGATTACGCTTTCCAGTGGGGAAGCGAAGCAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCGGATGTATCTACGtt                                                                                                        >  1:364976/1‑140 (MQ=255)                          tGATTACGCTTTCCAGTGGGGAAGCGAAGCAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCGGATGTATCTACGTTGt                                                                                                      <  1:737491/140‑1 (MQ=255)                              tACGCTTTCCAGTGGGGAAGCGAAGCAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCGGATGTATCTACGTTGTCAgg                                                                                                  <  1:832555/140‑1 (MQ=255)                                cGCTTTCCAGTGGGGAAGCGAAGCAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCGGATGTATCTACGTTGTCAgggg                                                                                                <  1:286885/140‑1 (MQ=255)                                     tCCAGTGGGGAAGCGAAGCAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGc                                                                                           >  1:406148/1‑140 (MQ=255)                                               aagcgaagcAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGaa                                                                                 >  2:53388/1‑140 (MQ=255)                                               aagcgaagcAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGaa                                                                                 >  1:152004/1‑140 (MQ=255)                                                 gcgaagcAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAAc                                                                               <  1:202958/140‑1 (MQ=255)                                                      gcAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGc                                                                          <  2:892405/140‑1 (MQ=255)                                                      gcAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGc                                                                          <  2:74652/140‑1 (MQ=255)                                                                      tataTTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTccc                                                          >  2:368601/1‑140 (MQ=255)                                                                             aaCCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAgcg                                                   >  2:824125/1‑140 (MQ=255)                                                                              aCCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAgcgc                                                  >  1:298325/1‑140 (MQ=255)                                                                                ccATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCg                                                >  2:532884/1‑140 (MQ=255)                                                                                  aTCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCgaa                                              >  2:398168/1‑138 (MQ=255)                                                                                  aTCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGa                                              >  1:365887/1‑140 (MQ=255)                                                                                  aTCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGa                                              >  2:171101/1‑140 (MQ=255)                                                                                  aTCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGa                                              >  1:593840/1‑140 (MQ=255)                                                                                  aTCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGa                                              >  1:377022/1‑140 (MQ=255)                                                                                  aTCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGa                                              >  2:415304/1‑140 (MQ=255)                                                                                    cGATGGTTATCGTATTCAGTTTGACTGGCATCCGACTTCGGACTCCACTGATCAGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATa                                            <  1:711453/140‑1 (MQ=255)                                                                                         gTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGt                                       <  1:549371/140‑1 (MQ=255)                                                                                         gTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGt                                       <  1:361843/140‑1 (MQ=255)                                                                                         gTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGt                                       <  2:629889/140‑1 (MQ=255)                                                                                           tATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCa                                     <  2:558085/140‑1 (MQ=255)                                                                                           tATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCa                                     <  1:67945/140‑1 (MQ=255)                                                                                             tCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGt                                   <  2:298325/140‑1 (MQ=255)                                                                                                   tCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTg                             >  2:718497/1‑140 (MQ=255)                                                                                                       tttGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTgacga                         >  1:36786/1‑140 (MQ=255)                                                                                                       tttGACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTgacga                         >  1:630428/1‑140 (MQ=255)                                                                                                          gACTGGTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACcg                      >  1:909958/1‑140 (MQ=255)                                                                                                               gTATCCGACTTCGGACTCCACTGATCAGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGtga                 >  1:541584/1‑140 (MQ=255)                                                                                                                         tCGGACTCCACTGATCAGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATc       >  1:869161/1‑140 (MQ=255)                                                                                                                          cGGACTCCACTGATCAGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCg      >  1:486679/1‑140 (MQ=255)                                                                                                                          cGGACTCCACTGATCAGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCg      >  1:354664/1‑140 (MQ=255)                                                                                                                              cTCCACTGATCAGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGtttt  <  2:630428/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                 GCCGCGCCAAAAGGCATTGAGCCGGTGATTACGCTTTCCAGTGGGGAAGCGAAGCAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTT  >  NC_000913/1498254‑1498518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑