Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,111,7470GT30.9% 87.8 / 27.8 42N156K (AAC→AAA) yiiQDUF1454 domain‑containing protein YiiQ
Reads supporting (aligned to +/- strand):  ref base G (26/3);  new base T (0/13);  total (26/16)
Fisher's exact test for biased strand distribution p-value = 2.19e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01
Rejected as consensus: Frequency below/above cutoff threshold.

ATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGA  >  NC_000913/4111617‑4111883
                                                                                                                                   |                                                                                                                                        
ttttATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCCCCTTCTGTCTCGGTGTAATAACGTTTGTTTTTcccc                                                                                                                                 <  2:532887/139‑1 (MQ=255)
 tttATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCTGTTCAACAGCGAAGGTCAGCCCCTTTT‑CTCCGTTGTCCGCGTCAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCccg                                                                                                                                >  2:1466519/1‑140 (MQ=255)
      tCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGtt                                                                                                                           >  1:1983452/1‑140 (MQ=255)
          aCCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTtagta                                                                                                                       >  2:1869404/1‑140 (MQ=255)
                    gtttCAGATAGCGCCAGTTAAATGGGTTCAACAGCGAAGGCCACCCCCTTTC‑CGCCTTTGCCCGCGACAACATACCGCGGTCCCCCTTCTGTCTGGGTTTAATACCTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAg                                                                                                             <  2:1977908/138‑1 (MQ=255)
                      ttCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGtt                                                                                                           >  1:1402296/1‑140 (MQ=255)
                               cgcCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGc                                                                                                  >  1:86053/1‑140 (MQ=255)
                                gcCAGCTTAATCGGTTCAACGGGGAGGGTCCGCCCTTTTT‑CGCTGTTGCCCGCGACAACATAACGCAGTGCCCCTTCTTTTGGGGTGTAATACCGTTTTTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCt                                                                                                 <  2:86053/140‑1 (MQ=255)
                                gcCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCt                                                                                                 >  1:1664667/1‑140 (MQ=255)
                                gcCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCt                                                                                                 >  1:985502/1‑140 (MQ=255)
                                gcCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCt                                                                                                 >  1:789346/1‑140 (MQ=255)
                                 ccAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCtt                                                                                                >  1:440559/1‑140 (MQ=255)
                                            gttcaaaCAGCGAGGGTCACCCCTTTTC‑CGCCGTTGTCCGCAAAAACAAACCGCGGTGCCCTTTCTGTCTGGGTGTAATACCTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGt                                                                                     <  2:1664667/135‑1 (MQ=255)
                                              ttCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCa                                                                                   >  1:1920401/1‑140 (MQ=255)
                                              gacagcAGCGAGGGTCCGCCCCTTTC‑CGCTGTGGCCCGCAAAAACAGAGCTCGGTCCCCCTTTTTGCTGGGTTTATTATCGTTTTTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCa                                                                                   <  2:1645569/135‑1 (MQ=255)
                                                  aaggcaaaGGTCACCCCTTTTT‑CGCCGTTGTCCGCGAAAAATTAACGCGGTGCACTTTCTGTCTGGGTGTAATACTTTTTTTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTaa                                                                               <  2:374748/134‑1 (MQ=255)
                                                  aaaGCGACGGCCACCCCTTTTC‑CGCCGTTGCCCGGAACAAAAAAACGCGGTGCACTTTCTGTCTGGGTAAAATAATGTTTTTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTaa                                                                               <  2:1272796/138‑1 (MQ=255)
                                                  aCAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTaa                                                                               >  1:1672233/1‑140 (MQ=255)
                                                        aaGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGt                                                                         >  1:1750231/1‑140 (MQ=255)
                                                        aaGGTCACCCCCTTTC‑CGCCGTTGCCCGCAAAAAAATACCGCATTCCCCCTTCTGTCTGGGTGTAATACTGTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGt                                                                         <  2:900922/140‑1 (MQ=255)
                                                         aaggcccccccTTTTCCGCCGTGGTCCGCGACAACATACCGCGTTCCCCCTTCTGTCTGGGTGTAATAATTTTTTTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGt                                                                         <  2:166999/133‑1 (MQ=255)
                                                              aGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTgctgtc                                                                   >  1:1645569/1‑135 (MQ=255)
                                                                  ccTTTT‑CGCCGTTGTCCCCGACAACATAACGCAGTGCCCCTTCTGTCTGGGTGTAATAACTTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCgg                                                               <  2:1983452/140‑1 (MQ=255)
                                                                    tttt‑CGCCGTTGCCCGCAACAACATACCGCGGTGCCCTTTCTGTCTGGGTTTAATAATGTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGAt                                                             <  2:914956/140‑1 (MQ=255)
                                                                           ccGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGc                                                       >  2:1476524/1‑140 (MQ=255)
                                                                            tgTTGCCCGCGAAAAAATACCGCAGTCCCCCTTCTGTCTGGGTGTAATAACGTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCt                                                      <  1:1447027/139‑1 (MQ=255)
                                                                             gTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTg                                                     >  2:1265676/1‑140 (MQ=255)
                                                                             gTTGCCCGCGAAAAAATACCGTAGTCCTCCTTCGGTCGGGTTATATTAGTTTTTTTTTTCCCCCGCCGTTAGTAGCGAGTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTg                                                     <  2:443363/140‑1 (MQ=255)
                                                                               tgcccGCAACAACATAACGCAGTGCCCCTTCTGTCTCGGTGTAATAACTTTTGTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGcc                                                   <  1:1272893/137‑1 (MQ=255)
                                                                                              accGCAGTCCCCTTTCTGTCTGGGTTTAATACCTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgct                                    <  1:541876/138‑1 (MQ=255)
                                                                                                     tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                             >  2:1030390/1‑140 (MQ=255)
                                                                                                     tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                             >  1:239410/1‑140 (MQ=255)
                                                                                                     tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                             >  2:611502/1‑140 (MQ=255)
                                                                                                            tCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGc                      >  1:444542/1‑140 (MQ=255)
                                                                                                            tCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGc                      >  1:2027945/1‑140 (MQ=255)
                                                                                                               ttttgggTGTAATATTTTTTGTTTACCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCttt                   <  2:2005814/135‑1 (MQ=255)
                                                                                                               gTCTGGGTTTAATACTGTTTTTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCttt                   <  2:1672233/140‑1 (MQ=255)
                                                                                                               gTCTGGGTGTAATAGTTTTTTTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCttt                   <  2:180627/140‑1 (MQ=255)
                                                                                                               gTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCttt                   >  1:363931/1‑140 (MQ=255)
                                                                                                               gTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCttt                   >  2:1479480/1‑140 (MQ=255)
                                                                                                                  ccggtaaaatacCTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTg                <  1:232604/133‑1 (MQ=255)
                                                                                                                    ggTGTAATAACTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGTGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGct              <  1:1072537/140‑1 (MQ=255)
                                                                                                                    ggTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGct              >  2:1892187/1‑140 (MQ=255)
                                                                                                                     gtgtAATAGGTTTTTTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGctc             <  1:614527/140‑1 (MQ=255)
                                                                                                                     gtgtAATAACGTTTGTTTTTCCCGGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGctc             >  1:847857/1‑140 (MQ=255)
                                                                                                                      tgtAATAACTTTTTTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGctct            <  1:791146/140‑1 (MQ=255)
                                                                                                                      tgtAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGctct            <  1:1030390/140‑1 (MQ=255)
                                                                                                                        taataaCGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTgg          >  1:1052154/1‑140 (MQ=255)
                                                                                                                          ataaCGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCTTGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGcc        >  1:985024/1‑140 (MQ=255)
                                                                                                                          ataaCGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGcc        >  1:788601/1‑140 (MQ=255)
                                                                                                                          aaagctttttttttCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGcc        <  1:1667517/134‑1 (MQ=255)
                                                                                                                              ttttttttttCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTg    <  1:1869404/138‑1 (MQ=255)
                                                                                                                              cGTTTTTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTg    <  1:611502/140‑1 (MQ=255)
                                                                                                                              cGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTg    <  1:1265676/140‑1 (MQ=255)
                                                                                                                                tttgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGa  >  2:1110535/1‑140 (MQ=255)
                                                                                                                                tttgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGa  >  1:642041/1‑140 (MQ=255)
                                                                                                                                tttgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGa  >  1:1865370/1‑140 (MQ=255)
                                                                                                                                tttgtttTTCCCCGCCATTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGa  >  1:870931/1‑140 (MQ=255)
                                                                                                                                   |                                                                                                                                        
ATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGA  >  NC_000913/4111617‑4111883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑