Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,568,5040TG32.4% 296.8 / 14.3 141T6P (ACC→CCC) mdtMmultidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter
Reads supporting (aligned to +/- strand):  ref base T (26/68);  new base G (45/0);  total (73/68)
Fisher's exact test for biased strand distribution p-value = 2.49e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 149 total aligned reads displayed.

AGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTAAT  >  NC_000913/4568374‑4568639
                                                                                                                                  |                                                                                                                                         
aGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACg                                                                                                                                  <  2:428367/140‑1 (MQ=255)
aGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACg                                                                                                                                  <  2:271975/140‑1 (MQ=255)
aGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACg                                                                                                                                  <  1:2009220/140‑1 (MQ=255)
  aCTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTg                                                                                                                                <  2:1241923/140‑1 (MQ=255)
     gACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCa                                                                                                                             <  1:1259574/140‑1 (MQ=255)
     gACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCa                                                                                                                             >  1:2027060/1‑140 (MQ=255)
     gACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCa                                                                                                                             <  2:1422321/140‑1 (MQ=255)
       cATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATa                                                                                                                           >  1:1642477/1‑140 (MQ=255)
          cGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATaaca                                                                                                                        <  1:873868/140‑1 (MQ=255)
             cATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCATaaaaaaa                                                                                                                     >  1:1165858/1‑139 (MQ=255)
                   aaTCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTcc                                                                                                               <  1:513654/140‑1 (MQ=255)
                   aaTCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTcc                                                                                                               <  2:551845/140‑1 (MQ=255)
                          tACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCCTAAAAAACAAAACCTTTTccc                                                                                                        >  2:366030/1‑139 (MQ=255)
                          tACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGCAAAAAAAAAAGGCATAAAAAAAAACTCCCTGTTCg                                                                                                        >  2:1859826/1‑140 (MQ=255)
                            ccACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCATAACAAAAAAATCCTTGTCCGga                                                                                                      >  1:1756784/1‑140 (MQ=255)
                             cacaTTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGgag                                                                                                     <  1:1697047/140‑1 (MQ=255)
                               caTTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGGGGGATAAAAAAAAAATCCTTGTCCGGAG‑aa                                                                                                  >  1:1443782/1‑140 (MQ=255)
                                 agacagATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑cc                                                                                               <  1:1534037/135‑1 (MQ=255)
                                  tAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑ccc                                                                                              >  1:218351/1‑140 (MQ=255)
                                       aTCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAGATGCCTAACCAACAACACCCTGTCCGGAG‑AA‑ACCCCAcc                                                                                         >  1:377840/1‑139 (MQ=255)
                                       aTCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACg                                                                                         >  1:990665/1‑140 (MQ=255)
                                       aTCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACg                                                                                         >  1:1957322/1‑140 (MQ=255)
                                       aTCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGAGGCATAACAAAAAACTCCTTGTCCGGAG‑AA‑CCCCaaaa                                                                                         >  2:1697047/1‑138 (MQ=255)
                                       aTCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGAGGCATAAAAAAAAACTCCCTGTCCGGAG‑AA‑CCCCAAcc                                                                                         >  2:1373355/1‑139 (MQ=255)
                                         cccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACggg                                                                                       <  1:427255/140‑1 (MQ=255)
                                         cccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAGTGGCATAAAAAAAAACTCCTTTTCCGGAG‑AA‑CCCCAACcgg                                                                                       >  1:109932/1‑140 (MQ=255)
                                          ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAGTGGCATAAAAAACAACCCCTTTTCCGGGG‑AA‑CCCCCACgggg                                                                                      >  2:1309897/1‑140 (MQ=255)
                                          ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAACTGGCATAAAAAAAAACTCCTTTTCCCGGG‑AACCCCCAACggg                                                                                       >  2:781095/1‑140 (MQ=255)
                                          ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAATGGGGTAAAAAAAAAAACCCTTTCCCGAG‑AA‑ACCCAACgggg                                                                                      >  2:1316070/1‑140 (MQ=255)
                                          ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAACGAGGGATAACAAAAAACTCCCTGTCCGGGG‑GA‑CCCCCACcggg                                                                                      >  2:749140/1‑140 (MQ=255)
                                          ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCATAACAAAAAACCCCTTTTTCCGGG‑AA‑ACCCAAAgggg                                                                                      >  2:471075/1‑140 (MQ=255)
                                          ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCATAAAAAAAAACTCCTTGTCCCGAG‑AA‑ACCCCACgggg                                                                                      >  2:1332896/1‑140 (MQ=255)
                                          ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAACAGGCGTAAAAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACgggg                                                                                      >  2:262130/1‑140 (MQ=255)
                                           cAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGa                                                                                     <  2:1200138/140‑1 (MQ=255)
                                           cAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGa                                                                                     <  2:1166926/140‑1 (MQ=255)
                                            aGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAGGTAGCACAAAAAAAAAAGTCTTGTTCGGAG‑GA‑CCCCCACggggga                                                                                    >  2:1764100/1‑138 (MQ=255)
                                            aGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGGGGCATAAAAAAAAACTCCTTTTCCGGAG‑AA‑CCCCAACggggga                                                                                    >  1:615857/1‑138 (MQ=255)
                                              gCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCATAACAAACAACTCCCTGTCCAGAG‑AA‑CCCCCACGGGGgaaa                                                                                  >  1:1589430/1‑140 (MQ=255)
                                                tGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACg                                                                                <  1:1576346/140‑1 (MQ=255)
                                                tGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACg                                                                                <  1:1376019/140‑1 (MQ=255)
                                                  gATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACg                                                                                >  1:721929/1‑138 (MQ=255)
                                                  gATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACg                                                                                <  2:721929/138‑1 (MQ=255)
                                                  gATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAGTGGCATAAAAAACAACCCCTTTTCCGGAG‑AA‑CCCCAAAGGGGGAAAAGaa                                                                              >  2:2052495/1‑140 (MQ=255)
                                                  gATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGGAAAAAACATGGCCTAACAAAAAACTCCCCGTCCGGAA‑AA‑ACCCCACGGGGAAAAAGaa                                                                              >  2:619094/1‑140 (MQ=255)
                                                  gATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCATAAAAAAAAACTCCTTGTCCGGAG‑AA‑ACCCCACGGGGAAAAAGaa                                                                              >  2:1508785/1‑140 (MQ=255)
                                                   aTCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAGGGGCATAAAAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGaaaaaaaaa                                                                             >  2:594124/1‑135 (MQ=255)
                                                   aTCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCATAAAAAAAAACTCCTTGTTCGGAG‑AA‑CCCCAACGGGGGAAACGaaa                                                                             >  2:109350/1‑140 (MQ=255)
                                                        aTCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAGTGGCATAAAAAAAAACCCCTTGTCCGGGG‑AA‑ACCCAACCGGGGAAAAAAAAATCaa                                                                        >  2:1984771/1‑140 (MQ=255)
                                                        aTCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGGGGCATAAAAAACAAATCCTTGTCCGGAG‑AA‑ACCCCACGGGGAAAAAGAAAATCaa                                                                        >  2:955456/1‑140 (MQ=255)
                                                        aTCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGCGGGATAAAAAAAAAACCCTTGTCCGGAG‑AA‑ACCCAACGGGGGAAAAGAAAATCaa                                                                        >  2:818536/1‑140 (MQ=255)
                                                         tCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACGAAATTCAAt                                                                       <  1:471075/140‑1 (MQ=255)
                                                          ccGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACGAAATTCAATa                                                                      <  2:1434949/140‑1 (MQ=255)
                                                            gTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACACCCTGTCCGGAG‑AA‑CCCCAAAGGGGAAAACGAAATTCAATAcc                                                                    >  2:408011/1‑140 (MQ=255)
                                                             tCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAAAGGCATAAAAAAAAAACCCCTGTCCGGGGAAA‑CCCCACCGGGGAAAA‑GAAAATCAAAAccc                                                                   >  2:1724796/1‑139 (MQ=255)
                                                             tCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGAGGCATAAAAAACAACTCCCTGTCCGGAG‑AA‑CCCCCACGGGGAAAACGAAAATCaaaaacc                                                                   >  2:1840615/1‑135 (MQ=255)
                                                             tCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGAGGCATAAAAAAAAACCCCTCGGCCCGGG‑GA‑CCCCAAAAGGGGAAAAGAAAATCAATAccc                                                                   >  2:1734589/1‑139 (MQ=255)
                                                             tCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAATGGCATAAAAAAAAACTCCCTGTCCGGAG‑AA‑ACCCCACGGGGGAAAAGAAAATCAATAccc                                                                   >  2:357837/1‑139 (MQ=255)
                                                             tCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGCAAAAAAAAGTGGCCTAAAAAAAAAAACCCTGTCCGGAGAAA‑CCCCAACGGGGAAAA‑GAAAATCAAAAccc                                                                   >  2:820190/1‑139 (MQ=255)
                                                               gACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAATGGCATAAAAAACAACTCCTTGTCCGGGG‑AA‑CCCCAACGGGGAAAAAGAAAATCAAAACCGTc                                                                 >  2:778134/1‑140 (MQ=255)
                                                                  aGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACg                                                                                <  2:1373993/122‑1 (MQ=255)
                                                                    aTACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTaa                                                            <  1:440651/140‑1 (MQ=255)
                                                                    aTACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTaa                                                            <  1:109350/140‑1 (MQ=255)
                                                                    aTACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTaa                                                            <  1:998028/140‑1 (MQ=255)
                                                                    aTACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAGTGGGATAAAAAACAACTCCTTGTCCGGAG‑AA‑ACCCCACGGGGGAAAAGAAAATCAATACCGTCGGGaa                                                            >  2:440651/1‑140 (MQ=255)
                                                                       cGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAACGGGGCATAAAAAAAAAACCCTTGTCCGGAG‑AA‑CCCCAAAGGGGGAAACGAAAATCAATAACGTCGGGAATGa                                                         >  2:1586426/1‑140 (MQ=255)
                                                                         cagcaAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACg                                                       <  1:408011/140‑1 (MQ=255)
                                                                            caAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGaaa                                                    <  2:2014930/140‑1 (MQ=255)
                                                                            caAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGaaa                                                    <  1:1373355/140‑1 (MQ=255)
                                                                              aaGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAgg                                                  <  1:1859826/140‑1 (MQ=255)
                                                                              aaGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAgg                                                  <  1:1201781/140‑1 (MQ=255)
                                                                              aaGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAGGGGCATAACAAACAACTCCTTGTCCGGGG‑AA‑CCCCAACGGGGAAAAAGAAAATCAATACCCGCGGGAATGACGaaaag                                                  >  2:1985283/1‑138 (MQ=255)
                                                                               aGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAGTGGCCTAAAAAAAAACACCCTTTCCGGGG‑AA‑ACCCAACAGGGGAAAAGAAATTTAATACCCCCGGGAATGACGAAAAGc                                                 >  2:958514/1‑140 (MQ=255)
                                                                                 tCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAg                                               <  1:1885364/140‑1 (MQ=255)
                                                                                 tCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAg                                               <  1:1720322/140‑1 (MQ=255)
                                                                                    tACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTg                                            <  1:1309897/140‑1 (MQ=255)
                                                                                    tACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCATAAAAAAAAAATCCCTTTCCGGAG‑AA‑CCCCAACGGGGAAAAAGAAAATCAAAACCGTCGGGAATGACGAAAAGCAgggg                                            >  2:1207791/1‑138 (MQ=255)
                                                                                      cAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTgag                                          <  1:1753428/140‑1 (MQ=255)
                                                                                      cAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTgag                                          <  1:345445/140‑1 (MQ=255)
                                                                                       aaaaTCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTgaga                                         <  1:82198/140‑1 (MQ=255)
                                                                                         aaTCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAg                                       <  2:218351/140‑1 (MQ=255)
                                                                                          aTCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGGGGCATAACAAAAAACCCCCTGTCCGGAG‑AA‑ACCCAACCGGGGAAAAGAAAATCAAAAACCTCGGGAAAGACGAAAAGCAGGGGAGAAgg                                      >  2:1079148/1‑140 (MQ=255)
                                                                                           tCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAggg                                     >  1:693779/1‑140 (MQ=255)
                                                                                           tCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGGGGCATAAAAAAAAACTCCTTGTCCGGAG‑AA‑ACCCAACGGGGGAAACGAAAATCAATACCCTCGGTAATGACGAAAAGCAGGGGAGAAagg                                     >  2:1364416/1‑140 (MQ=255)
                                                                                                 ccATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACga                               <  1:652917/140‑1 (MQ=255)
                                                                                                  cATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACgag                              <  1:1376038/140‑1 (MQ=255)
                                                                                                    tCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGAc                            <  2:1642477/140‑1 (MQ=255)
                                                                                                     cGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAGCGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCCACGGGGGAAACGAAAATCAAAACCGTCGGTAATGACGAAAAGCAGGGGAGAAGGGGAAACAGACt                           >  2:1600659/1‑140 (MQ=255)
                                                                                                           aaaCAGCGTGGCGGCATGGCGGGTAAAAAAACGTTGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAAt                     <  1:854401/140‑1 (MQ=255)
                                                                                                              cAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTa                  <  1:1840615/140‑1 (MQ=255)
                                                                                                              cAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTa                  <  1:1207791/140‑1 (MQ=255)
                                                                                                               aGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTAc                 <  1:1333891/140‑1 (MQ=255)
                                                                                                                gCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCCTAAAAAACAAACCCCTGTCCGGAG‑AA‑ACCCAACGGGGGAAACGAAATTTAATAACCACGGGAATGAGCAAAGACAAGGGAGAAGGGCGAACAGACTGCAAAAGGACt                >  2:1513890/1‑140 (MQ=255)
                                                                                                                 cGTGGCGGCATGACGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGCCGGTTATGACGAAAAGCAGGCGAGAAGGGCAACGAGACTTCAAATGTACTg               >  2:2016514/1‑140 (MQ=255)
                                                                                                                    ggcggcATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAAt            >  1:1436959/1‑140 (MQ=255)
                                                                                                                       ggcATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTc         <  1:820190/140‑1 (MQ=255)
                                                                                                                          aTGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATg      <  2:1764800/140‑1 (MQ=255)
                                                                                                                           tgccGGGTAAAAAACCGTGG‑ATAACAAACCACCTCCTGGCCGGAG‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTa    <  2:409389/137‑1 (MQ=255)
                                                                                                                            ggCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTa    <  1:259540/140‑1 (MQ=255)
                                                                                                                            ggCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTCCAAATGTACTGAATTTCATGTa    <  1:217582/140‑1 (MQ=255)
                                                                                                                              cGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTAAt  <  2:1165858/140‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                         
AGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTAAT  >  NC_000913/4568374‑4568639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑