Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,3440CT43.4% 77.8 / 74.3 99S62F (TCT→TTT) bglJDNA‑binding transcriptional regulator BglJ
Reads supporting (aligned to +/- strand):  ref base C (17/39);  new base T (43/0);  total (60/39)
Fisher's exact test for biased strand distribution p-value = 2.77e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 117 total aligned reads displayed.

AAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAT  >  NC_000913/4604205‑4604481
                                                                                                                                            |                                                                                                                                          
aaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑Ttttttc                                                                                                                                            <  2:2030679/140‑1 (MQ=255)
  aTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCTGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑Ttttttttt                                                                                                                                          >  1:1324271/1‑140 (MQ=255)
       tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTttttctttttct                                                                                                                                     <  2:692831/140‑1 (MQ=255)
       tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTttttctttttct                                                                                                                                     <  2:797111/140‑1 (MQ=255)
        cATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑Ttttttctttttttt                                                                                                                                    >  1:1112005/1‑137 (MQ=255)
         aTGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGc                                                                                                                                   <  1:578191/140‑1 (MQ=255)
         aTGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGc                                                                                                                                   <  2:1752552/140‑1 (MQ=255)
          tGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGcc                                                                                                                                  >  1:1414522/1‑140 (MQ=255)
                 taTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGaa                                                                                                                           >  2:762267/1‑140 (MQ=255)
                  aTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGGA‑TTTTTTTTTTTTTTTTCATGAGAAg                                                                                                                          >  2:1862500/1‑140 (MQ=255)
                   tCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGt                                                                                                                         <  2:1239808/140‑1 (MQ=255)
                   tCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATA‑CAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTTCCATGAGAAggg                                                                                                                        >  2:1470186/1‑138 (MQ=255)
                      gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTGCCATGAGGAGTggg                                                                                                                      >  2:530894/1‑138 (MQ=255)
                        aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTTA‑TTTTTTTTTTTTTTGACATGAGaaaggaacg                                                                                                                    >  2:944722/1‑133 (MQ=255)
                        aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAgcg                                                                                                                    <  2:1175873/140‑1 (MQ=255)
                         ttGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTTCCATGAGAAGGGAgcgc                                                                                                                   >  2:89670/1‑140 (MQ=255)
                          tGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCa                                                                                                                  <  2:17287/140‑1 (MQ=255)
                           gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCag                                                                                                                 <  1:762267/140‑1 (MQ=255)
                           gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCag                                                                                                                 >  1:710242/1‑140 (MQ=255)
                            agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTTTGCCATGAGAAGTGAGCGCaga                                                                                                                >  2:1043663/1‑140 (MQ=255)
                            agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCaga                                                                                                                <  1:1900639/140‑1 (MQ=255)
                             gagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                                                                               <  1:1916664/140‑1 (MQ=255)
                             gagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                                                                               <  2:1589231/140‑1 (MQ=255)
                             gagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                                                                               <  1:818163/140‑1 (MQ=255)
                                 ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTTAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTCTGCCATGAGAAGGGGGGGCAGAGAggg                                                                                                           >  1:628886/1‑140 (MQ=255)
                                 ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCT‑TTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCAGAGAggg                                                                                                           >  1:1964643/1‑140 (MQ=255)
                                 ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTCGCATGAGGAAGGGGCGCAGAGAggg                                                                                                           >  1:410197/1‑140 (MQ=255)
                                 ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAggg                                                                                                           <  1:828423/140‑1 (MQ=255)
                                 ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAggg                                                                                                           <  2:217834/140‑1 (MQ=255)
                                   aTTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCAGAGAggggt                                                                                                         >  1:2056473/1‑138 (MQ=255)
                                   aTTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCAGAGAggggt                                                                                                         >  2:256382/1‑138 (MQ=255)
                                     tCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagaga                                                                                                              >  1:745519/1‑133 (MQ=255)
                                     tCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagaga                                                                                                              <  2:745519/133‑1 (MQ=255)
                                         aaaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCtt                                                                                                   >  1:1962196/1‑140 (MQ=255)
                                          aaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTg                                                                                                  >  1:1830054/1‑140 (MQ=255)
                                          aaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTg                                                                                                  <  2:710242/140‑1 (MQ=255)
                                           aGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGc                                                                                                 <  1:754948/140‑1 (MQ=255)
                                             tttGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCct                                                                                               <  1:979738/140‑1 (MQ=255)
                                             tttGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCct                                                                                               <  1:1803421/140‑1 (MQ=255)
                                                     tAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAc                                                                                       <  2:925278/140‑1 (MQ=255)
                                                         ccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGc                                                                                   <  2:1962196/140‑1 (MQ=255)
                                                          cTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGGGGGAGTATCTTGTCTGACTGAACttggg                                                                                  >  2:1364996/1‑136 (MQ=255)
                                                             ttaAGCTCCATTCCTATTCCAGTTAGGAGTTATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGAtt                                                                               <  2:161215/139‑1 (MQ=255)
                                                             ataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGAtt                                                                               >  1:1041873/1‑140 (MQ=255)
                                                             ataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGAtt                                                                               <  1:1671606/140‑1 (MQ=255)
                                                                   tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTTCCTGACTGAACTGGGGGTTAAG‑ttt                                                                        >  2:899358/1‑140 (MQ=255)
                                                                   tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTTTGCCATGAGAAGTGAGCGCAGAGGGGGGTTATCTTGCCTGACTGAACTGGCGATTAAG‑ttt                                                                        >  2:582515/1‑140 (MQ=255)
                                                                   tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑ttt                                                                        >  1:1147024/1‑140 (MQ=255)
                                                                   tCCATACCTATACCAGACAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTCTTCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTTCCTGACTGAAATGGCGATTAAG‑ttt                                                                        >  2:1819088/1‑140 (MQ=255)
                                                                     cATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGATTAAG‑ttttc                                                                      >  2:1141406/1‑138 (MQ=255)
                                                                       tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCcgc                                                                    <  2:1147024/140‑1 (MQ=255)
                                                                       tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑ATTTTTTTTTTTTTTCCCTGAGAAAGGAGCGCAGAAAGGGGATATCTTGCCTTCCTGAACTGGCGATTAAA‑TTTTcgc                                                                    >  2:765592/1‑140 (MQ=255)
                                                                           tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTAc                                                                <  1:1926961/140‑1 (MQ=255)
                                                                            ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTGCCCTGGGAAGTGAGCGCAGAGAGGGGTTATCTTTCCTGACTGAACTGGCGGTTAAG‑TTTTCCCGTAcc                                                               >  2:1903177/1‑140 (MQ=255)
                                                                            ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCCGAGAGGGGGTATTTTGCCTGACTGAACTGGGGATTTAA‑TTTTCCCGTAcc                                                               >  2:2045251/1‑140 (MQ=255)
                                                                            ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTCTGCCATGAGAAGGGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTTCGCGGAcc                                                               >  2:6416/1‑140 (MQ=255)
                                                                            ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTAcc                                                               >  2:1365722/1‑140 (MQ=255)
                                                                             taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCC‑TTTTTTTTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTAccc                                                              >  2:673316/1‑140 (MQ=255)
                                                                             taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTCTGCCATGAGAAGTGAGCGCCGAGAGGGGTTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGGAccc                                                              >  2:1723323/1‑140 (MQ=255)
                                                                              aCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCg                                                             <  2:462399/140‑1 (MQ=255)
                                                                               ccAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCgg                                                            >  1:1102580/1‑140 (MQ=255)
                                                                                  gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCC‑TTTTTTTTTTTTTTTGCCTGAGGAATGAGCGGAGAGAGGGGTTATCTTGCCTGACTTAACTTGGGATTAAA‑ATTTCGCGGACCCGGCgg                                                         >  2:1468674/1‑139 (MQ=255)
                                                                                  gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCC‑TTTTTTCTTTTTCTGCCCTGAGAAGTGAGCGCAGAGAGGGAGTATCTTGCCTTACTGAAATGGCGGTTAAG‑GTTCCGCGTACCCcgcgg                                                         >  2:571844/1‑139 (MQ=255)
                                                                                  gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCAGAGAGGGGATATTTTGCCTGACTGAACTGGCGGTTAAG‑TTTCCGCGGACCCGGCgg                                                         >  2:653264/1‑139 (MQ=255)
                                                                                  gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTTCCATGAGAAGGGAGGGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGATTTAG‑TTTTCGCGTACCCGGCgg                                                         >  2:1025071/1‑139 (MQ=255)
                                                                                  gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTGCCATGAGAAGGGAGGGCCGAGGGGGGTTTTCTTGCCCGACTGAACCGGGGATTTAA‑TTTTCGCGGACCCGGCgg                                                         >  2:1029452/1‑139 (MQ=255)
                                                                                  gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTTTGCCATGAGAAGTGAGGGCAGGGAGGGGTTATTTTGCCTGACTCAAATCGCGGTTAAG‑GTTTCGCGGACCCcgcgc                                                         >  2:697645/1‑139 (MQ=255)
                                                                                  gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCg                                                          <  1:1195149/139‑1 (MQ=255)
                                                                                  gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCg                                                          >  2:1195149/1‑139 (MQ=255)
                                                                                   tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCT‑TTTTTTCTTTTTCTGTCATGAGGAGGGAGGGCGGAGAGGGATTATCTTGCCTGACTGCACTGGCGATTAAG‑ATTTCGCCGACCCCGccct                                                        >  2:1383331/1‑137 (MQ=255)
                                                                                   tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTTCGCGGACCCGGCGtt                                                        >  2:270857/1‑140 (MQ=255)
                                                                                   tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTTCCATGAGAAGGGAGCGCAGAGAGGGGTTATTTTGCCTGACTGAACTGGCGGTTAAG‑TTTCCGCGTACCCGGCGtt                                                        >  2:1104970/1‑140 (MQ=255)
                                                                                   tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGTCGtt                                                        >  2:1480411/1‑140 (MQ=255)
                                                                                   tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGtt                                                        >  1:923760/1‑140 (MQ=255)
                                                                                   tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGACATTTTTTTTTTTTTCGGCCA‑GCGGAAAGAGGGCCGAGGGGGGGTATTTTGTCTGGATGAACGGGGGAATAAGTTTTCCGCCAACCCgcgtt                                                         >  2:560435/1‑136 (MQ=255)
                                                                                    cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGTGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGttt                                                       >  2:258131/1‑140 (MQ=255)
                                                                                    cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGgtt                                                       >  2:137308/1‑140 (MQ=255)
                                                                                    cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGttt                                                       >  2:279736/1‑140 (MQ=255)
                                                                                    cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTGCCATGAGAAGGGAGCGCAGAGAGGGATTATTTTGCCTGACTGGACTGGCGATTAAA‑TTTCCGCGGACCCGGCGttt                                                       >  2:2019756/1‑140 (MQ=255)
                                                                                    cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTTCCTGACTGAACTGGCGATTAAG‑TTTTCGCGTACCCCGCGgtt                                                       >  2:975827/1‑140 (MQ=255)
                                                                                    cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGttt                                                       >  1:150527/1‑140 (MQ=255)
                                                                                          tCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTAt                                                 <  1:1750289/140‑1 (MQ=255)
                                                                                          tCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTAt                                                 <  1:692915/140‑1 (MQ=255)
                                                                                             tttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTTCCTGACTGAACTGGCGATTAAG‑TTTTCGCGTACCCGGCGTTTTGTTATTgg                                              >  2:1750289/1‑139 (MQ=255)
                                                                                              ttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCg                                             <  2:923760/140‑1 (MQ=255)
                                                                                                cAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGga                                           <  1:883661/140‑1 (MQ=255)
                                                                                                  ggATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGgatg                                         <  1:360416/140‑1 (MQ=255)
                                                                                                   gATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGgatga                                        <  1:1365722/140‑1 (MQ=255)
                                                                                                    aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGTCGTTTAGTTATTGCGgatgat                                       <  1:1480411/140‑1 (MQ=255)
                                                                                                    aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgat                                       <  1:1819088/140‑1 (MQ=255)
                                                                                                        cATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTTCCATGAAGAGTGAGTGCGGAGAGGGGTTATCTTTCCTGACTGAACTGGGGATTAAG‑TTTGCGCGGACCCGGCGGTTAGGTATTGCGGATGATGata                                   >  2:360416/1‑140 (MQ=255)
                                                                                                         aTGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGatat                                  <  1:256382/140‑1 (MQ=255)
                                                                                                                     tcGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCt                      <  1:1025071/140‑1 (MQ=255)
                                                                                                                            cggcggTCA‑TTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGATTAAG‑GTTTCGCGGACCCCGCGGTTTGTTATTTCGGGTGATGATATTTAAGCTCGGCTGATTGGt               >  2:940245/1‑140 (MQ=255)
                                                                                                                            cggcggTCA‑TTTTTTTTTTTTCTGCCCTGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGt               >  2:1720804/1‑140 (MQ=255)
                                                                                                                                ggTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCAt           <  1:975827/140‑1 (MQ=255)
                                                                                                                                 gTCA‑TTTTTTTTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCAtt          >  2:1413899/1‑140 (MQ=255)
                                                                                                                                 gTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGGGCAGAGAGGGATTTTCTTGCCTGACTGAACTGGCGGTTAAG‑TTTCCGCCGAACCCGCGTTTAGTTATTTCGGAAGATGATATTTAAGCTCGGCTGGTTGGTTCAtt          >  2:240019/1‑140 (MQ=255)
                                                                                                                                          ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGTGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt  <  1:1470186/140‑1 (MQ=255)
                                                                                                                                          ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt  <  2:1645464/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                          
AAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAT  >  NC_000913/4604205‑4604481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑