Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	25,738	C→A	intergenic (+37/‑88)	lspA → / → fkpB	lipoprotein signal peptidase/peptidyl‑prolyl cis‑trans isomerase FkpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	25,738	0	C	A	100.0%	202.9 / NA	60	intergenic (+37/‑88)	lspA/fkpB	lipoprotein signal peptidase/peptidyl‑prolyl cis‑trans isomerase FkpB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (27/33);  total (27/33)

ACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGC  >  NC_000913/25612‑25874                                                                                                                               |                                                                                                                                         aCCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATtg                                                                                                                             <  1:1869941/140‑1 (MQ=255)  ccTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATtgc                                                                                                                            >  1:1037250/1‑140 (MQ=255)     tCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGGGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATtgctgc                                                                                                                         >  2:1486913/1‑140 (MQ=255)      cAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCg                                                                                                                        <  1:110493/140‑1 (MQ=255)       aaCCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTAc                                                                                                                                   >  2:786428/1‑128 (MQ=255)       aaCCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTAc                                                                                                                                   <  1:786428/128‑1 (MQ=255)            tGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCg                                                                                                                  >  2:823985/1‑140 (MQ=255)             gCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGt                                                                                                                 >  2:899831/1‑140 (MQ=255)             gCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGt                                                                                                                 >  1:437335/1‑140 (MQ=255)              ccGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTa                                                                                                                >  2:443775/1‑140 (MQ=255)               cGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAg                                                                                                               <  1:2566/140‑1 (MQ=255)                      gCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAGCCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGAt                                                                                                        <  2:828155/140‑1 (MQ=255)                        cATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATaa                                                                                                      <  2:1037250/140‑1 (MQ=255)                             gtgtCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGt                                                                                                 >  1:1767965/1‑140 (MQ=255)                              tgtCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGtt                                                                                                >  1:1618992/1‑140 (MQ=255)                                 cGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTAc                                                                                             >  2:879447/1‑140 (MQ=255)                                    tGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTAcgcc                                                                                          >  1:298796/1‑140 (MQ=255)                                      cGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTAcgccgc                                                                                        >  1:1609541/1‑140 (MQ=255)                                      cGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTAcgccgc                                                                                        >  1:899566/1‑140 (MQ=255)                                       ggCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCa                                                                                       <  2:34440/140‑1 (MQ=255)                                        gCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCAt                                                                                      >  2:487778/1‑140 (MQ=255)                                            tGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATTCgg                                                                                  <  1:1025350/140‑1 (MQ=255)                                              aTTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCa                                                                                >  2:235343/1‑140 (MQ=255)                                               ttGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCaa                                                                               <  1:328347/140‑1 (MQ=255)                                                tgtgCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCaaa                                                                              <  2:553582/140‑1 (MQ=255)                                                     tGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATcc                                                                         <  1:1391214/140‑1 (MQ=255)                                                        aaGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTa                                                                      >  1:632832/1‑140 (MQ=255)                                                        aaGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTa                                                                      >  1:1983561/1‑140 (MQ=255)                                                        aaGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTa                                                                      >  2:811969/1‑140 (MQ=255)                                                             tttttGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAatat                                                                 <  2:1470645/140‑1 (MQ=255)                                                              ttttGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAAtata                                                                >  2:1113411/1‑140 (MQ=255)                                                              ttttGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAAtata                                                                >  2:1228949/1‑140 (MQ=255)                                                                  gCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAagag                                                            <  1:1970444/140‑1 (MQ=255)                                                                     ttCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCaa                                                         <  1:879447/140‑1 (MQ=255)                                                                     ttCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCaa                                                         <  2:899566/140‑1 (MQ=255)                                                                      tCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCaaa                                                        >  1:1787353/1‑140 (MQ=255)                                                                           agCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGc                                                   <  2:437335/140‑1 (MQ=255)                                                                           agCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGc                                                   <  2:1746001/140‑1 (MQ=255)                                                                           agCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGc                                                   <  1:487778/140‑1 (MQ=255)                                                                               aaaaaaCAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGt                                               >  1:494500/1‑140 (MQ=255)                                                                                aaaaaCAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTc                                              <  1:899831/140‑1 (MQ=255)                                                                                aaaaaCAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTc                                              <  2:1609541/140‑1 (MQ=255)                                                                                aaaaaCAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTc                                              <  2:592396/140‑1 (MQ=255)                                                                                aaaaaCAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTc                                              <  2:298796/140‑1 (MQ=255)                                                                                aaaaaCAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTc                                              <  1:1228949/140‑1 (MQ=255)                                                                                     cAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAAt                                         <  1:267407/140‑1 (MQ=255)                                                                                     cAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAAt                                         <  1:1486913/140‑1 (MQ=255)                                                                                          ataaACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTa                                    <  1:443775/140‑1 (MQ=255)                                                                                          ataaACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTa                                    <  1:823985/140‑1 (MQ=255)                                                                                          ataaACCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTa                                    <  1:658270/140‑1 (MQ=255)                                                                                              aCCCTGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACaga                                >  2:880384/1‑140 (MQ=255)                                                                                                  tGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTc                                              >  2:1045776/1‑122 (MQ=255)                                                                                                  tGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTc                                              <  1:1045776/122‑1 (MQ=255)                                                                                                  tGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCaa                            >  1:454613/1‑140 (MQ=255)                                                                                                  tGCCGGATGCGATGCTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCaa                            >  1:1734063/1‑140 (MQ=255)                                                                                                       gatgcgatgcTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAgcg                       <  1:1223201/140‑1 (MQ=255)                                                                                                       gatgcgatgcTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAgcg                       <  1:1113411/140‑1 (MQ=255)                                                                                                       gatgcgatgcTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAgcg                       <  1:811969/140‑1 (MQ=255)                                                                                                          gcgatgcTGACGCATCTTATCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCg                    <  2:494500/140‑1 (MQ=255)                                                                                                                            aTCAGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGc  >  1:362856/1‑140 (MQ=255)                                                                                                                               |                                                                                                                                         ACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGC  >  NC_000913/25612‑25874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑