Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,083,942	T→C	I396T (ATC→ACC)	sbcB →	exodeoxyribonuclease I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,083,942	0	T	C	95.4%	302.5 / 1.6	87	I396T (ATC→ACC)	sbcB	exodeoxyribonuclease I
Reads supporting (aligned to +/- strand):  ref base T (2/2);  new base C (45/38);  total (47/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

GCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATATCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTGCA  >  NC_000913/2083809‑2084077                                                                                                                                       |                                                                                                                                        gcgGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACttt                                                                                                                                   <  2:1415314/140‑1 (MQ=255) gcgGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACttt                                                                                                                                   <  1:185730/140‑1 (MQ=255)  cgGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACtttt                                                                                                                                  <  1:1349887/140‑1 (MQ=255)          gAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGTTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATaa                                                                                                                          <  1:1399649/140‑1 (MQ=255)          gAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATaa                                                                                                                          <  1:1806740/140‑1 (MQ=255)             ccGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACg                                                                                                                       <  2:537230/140‑1 (MQ=255)             ccGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACg                                                                                                                       <  1:1364224/140‑1 (MQ=255)                    cGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGaa                                                                                                                >  1:1421290/1‑140 (MQ=255)                     gCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGaaa                                                                                                               <  2:1964542/140‑1 (MQ=255)                          cAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATATCACTTTTGTTGATAAACGGATTGAAAAGCtg                                                                                                          >  1:1062240/1‑140 (MQ=255)                          cAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCtg                                                                                                          >  1:590781/1‑140 (MQ=255)                          cAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCtg                                                                                                          >  1:438536/1‑140 (MQ=255)                          cAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCtg                                                                                                          <  2:1203356/140‑1 (MQ=255)                          cAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCtg                                                                                                          <  1:1663085/140‑1 (MQ=255)                          cAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCtg                                                                                                          >  2:1657488/1‑140 (MQ=255)                          cAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCtg                                                                                                          <  2:438536/140‑1 (MQ=255)                          cAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCtg                                                                                                          >  2:226221/1‑140 (MQ=255)                          cAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCtg                                                                                                          <  1:1352740/140‑1 (MQ=255)                             aTAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCtgttg                                                                                                       <  2:327956/140‑1 (MQ=255)                               aaCGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCtg                                                                                                          >  2:881630/1‑135 (MQ=255)                               aaCGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCtg                                                                                                          <  1:881630/135‑1 (MQ=255)                               aaCGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTgttgtt                                                                                                     <  1:785485/140‑1 (MQ=255)                               aaCGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTgttgtt                                                                                                     <  1:1213468/140‑1 (MQ=255)                                aCGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTc                                                                                                    >  2:2004961/1‑140 (MQ=255)                                 cGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCa                                                                                                   >  2:1094483/1‑140 (MQ=255)                                 cGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCa                                                                                                   >  1:1587173/1‑140 (MQ=255)                                 cGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTCACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCa                                                                                                   >  1:1522556/1‑140 (MQ=255)                                    ggATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAAtt                                                                                                >  2:160335/1‑140 (MQ=255)                                       tGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATc                                                                                             >  2:210728/1‑140 (MQ=255)                                       tGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATc                                                                                             >  1:828162/1‑140 (MQ=255)                                       tGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATc                                                                                             >  2:1931680/1‑140 (MQ=255)                                          acaGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCggg                                                                                          <  2:1699743/140‑1 (MQ=255)                                           caGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGc                                                                                         >  2:973839/1‑140 (MQ=255)                                              cTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACg                                                                                      >  1:1260403/1‑140 (MQ=255)                                              cTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACg                                                                                      >  2:1128494/1‑140 (MQ=255)                                                ttATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATATCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCa                                                                                    <  2:1062240/140‑1 (MQ=255)                                                 tataACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCaa                                                                                   <  1:1927744/140‑1 (MQ=255)                                                   taACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACt                                                                                 >  2:1367009/1‑140 (MQ=255)                                                        gCTTTTTCAGTGACGCAGATCGTGGAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTccc                                                                             >  1:1428016/1‑140 (MQ=255)                                                        gCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATATCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCg                                                                            >  2:1506900/1‑140 (MQ=255)                                                        gCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCg                                                                            >  1:604421/1‑140 (MQ=255)                                                        gCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCg                                                                            >  2:2006388/1‑140 (MQ=255)                                                        gCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCg                                                                            >  1:1527910/1‑140 (MQ=255)                                                        gCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCg                                                                            >  1:780740/1‑140 (MQ=255)                                                        gCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCg                                                                            >  2:1857171/1‑140 (MQ=255)                                                        gCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCg                                                                            >  2:151459/1‑140 (MQ=255)                                                        gCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCg                                                                            >  2:1181670/1‑140 (MQ=255)                                                         cTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATATCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCgg                                                                           <  1:1506900/140‑1 (MQ=255)                                                         cTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTATTGTTCAATTATCGGGCACGCAACTTCCCgg                                                                           <  1:936766/140‑1 (MQ=255)                                                          tttttCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCggg                                                                          <  2:60790/140‑1 (MQ=255)                                                          tttttCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCggg                                                                          <  1:75177/140‑1 (MQ=255)                                                            tttCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGa                                                                        >  1:433655/1‑140 (MQ=255)                                                              tCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACg                                                                      <  2:5846/140‑1 (MQ=255)                                                              tCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACg                                                                      <  1:151459/140‑1 (MQ=255)                                                              tCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACg                                                                      <  1:1657488/140‑1 (MQ=255)                                                              tCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACg                                                                      <  1:1857171/140‑1 (MQ=255)                                                              tCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTCACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACg                                                                      <  2:1522556/140‑1 (MQ=255)                                                                aGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCt                                                                    >  1:1577690/1‑140 (MQ=255)                                                                         gATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGc                                                           <  2:1069389/140‑1 (MQ=255)                                                                           tCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCg                                                         <  1:1181670/140‑1 (MQ=255)                                                                              tGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGagc                                                      >  2:307441/1‑140 (MQ=255)                                                                              tGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGagc                                                      >  2:36554/1‑140 (MQ=255)                                                                                cagcaATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGagcag                                                    >  1:652969/1‑140 (MQ=255)                                                                                  gcaATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAgcagca                                                  <  2:780740/140‑1 (MQ=255)                                                                                     aTGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACg                                               <  1:226221/140‑1 (MQ=255)                                                                                      tGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACgc                                              >  1:458356/1‑140 (MQ=255)                                                                                      tGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACgc                                              >  2:394906/1‑140 (MQ=255)                                                                                       gAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACgct                                             <  2:284126/140‑1 (MQ=255)                                                                                        aaaaTTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACgctg                                            >  1:1002128/1‑140 (MQ=255)                                                                                             tgtgCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGctggctgg                                       >  1:655382/1‑140 (MQ=255)                                                                                               tgCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAg                                     <  1:210728/140‑1 (MQ=255)                                                                                               tgCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAg                                     >  1:753177/1‑140 (MQ=255)                                                                                               tgCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAg                                     >  1:1037443/1‑140 (MQ=255)                                                                                                gCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGc                                    >  2:928643/1‑140 (MQ=255)                                                                                                         cGAGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGcc                           >  2:773024/1‑140 (MQ=255)                                                                                                           aGCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAg                         >  2:112646/1‑140 (MQ=255)                                                                                                            gCCGCGTAATTTACC‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAgg                        >  2:1445974/1‑140 (MQ=255)                                                                                                                          c‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGcc                           >  1:1605694/1‑123 (MQ=255)                                                                                                                          c‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGcc                           <  2:1605694/123‑1 (MQ=255)                                                                                                                          c‑GGCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCACGCCagag          >  2:1471041/1‑140 (MQ=255)                                                                                                                             gCACTGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCACGCCagaggt        <  1:112646/140‑3 (MQ=255)                                                                                                                                 tGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTg    <  1:443815/140‑1 (MQ=255)                                                                                                                                 tGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTg    <  2:1421290/140‑1 (MQ=255)                                                                                                                                 tGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTg    <  1:1931680/140‑1 (MQ=255)                                                                                                                                 tGGATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTACACGCCAGAGTTTTTg    <  2:1587173/140‑1 (MQ=255)                                                                                                                                   gATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTGCa  >  2:570682/1‑140 (MQ=255)                                                                                                                                   gATACCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTGCa  >  1:2040698/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        GCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACC‑GGCACTGGATATCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTGCA  >  NC_000913/2083809‑2084077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑