BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,384,163	C→A	A36S (GCT→TCT)	mdh ←	malate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,384,163	0	C	A	99.4%	551.6 / ‑3.7	157	A36S (GCT→TCT)	mdh	malate dehydrogenase
Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (72/84); total (72/85)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

Only 100 of 166 total aligned reads displayed.

ACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAG  >  NC_000913/3384026‑3384299

aCGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGgagaga                                                                                                                                        >  1:1603653/1‑140 (MQ=255)

aCGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTAAGATCGACAGCCACACCGGGAGTCACTGgagaga                                                                                                                                        >  2:2002679/1‑140 (MQ=255)

 cgcgCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGat                                                                                                                                       >  1:1774072/1‑140 (MQ=255)

 cgcgCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGat                                                                                                                                       <  2:568002/140‑1 (MQ=255)

  gcgcTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGata                                                                                                                                      <  2:977935/140‑1 (MQ=255)

    gcTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATc                                                                                                                                    <  2:369603/140‑1 (MQ=255)

     cTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCa                                                                                                                                   <  1:1395123/140‑1 (MQ=255)

       aCGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATa                                                                                                                                 <  2:76032/140‑1 (MQ=255)

       aCGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATa                                                                                                                                 >  2:775636/1‑140 (MQ=255)

        cGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATAc                                                                                                                                <  2:1893170/140‑1 (MQ=255)

           cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACaga                                                                                                                             <  1:720891/140‑1 (MQ=255)

             gCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACagaga                                                                                                                           <  2:342120/140‑1 (MQ=255)

             gCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACagaga                                                                                                                           <  2:2022889/140‑1 (MQ=255)

              catagaTAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCATCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGat                                                                                                                                       <  2:1909536/124‑1 (MQ=255)

              catagaTAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCATCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGat                                                                                                                                       >  1:1909536/4‑127 (MQ=255)

              cAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACagagag                                                                                                                          >  2:1863421/1‑140 (MQ=255)

              cAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACagagag                                                                                                                          >  2:1007802/1‑140 (MQ=255)

              cAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACagaga                                                                                                                           >  2:1598763/1‑139 (MQ=255)

                  gaTAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACagagagag                                                                                                                        <  2:115170/138‑1 (MQ=255)

                  gaTAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACagagagag                                                                                                                        >  1:115170/1‑138 (MQ=255)

                   aTAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGat                                                                                                                                       >  1:933160/1‑122 (MQ=255)

                   aTAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGat                                                                                                                                       <  2:933160/122‑1 (MQ=255)

                   aTAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTc                                                                                                                     >  2:1092139/1‑140 (MQ=255)

                        aaCGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAAc                                                                                                                >  1:1734023/1‑140 (MQ=255)

                           gACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTg                                                                                                             >  2:1679167/1‑140 (MQ=255)

                             cATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGaa                                                                                                           >  2:604226/1‑140 (MQ=255)

                             cATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGaa                                                                                                           >  1:258/1‑140 (MQ=255)

                             cATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGaa                                                                                                           >  1:1840729/1‑140 (MQ=255)

                             cATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGaa                                                                                                           >  1:1717111/1‑140 (MQ=255)

                                    gcCTTCCAGCGCCTGGGTCGCATCTTCACCAGAAATACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGGGAGAGTTCTGAACCTGAAGGCAGtt                                                                                                    <  1:2038404/140‑1 (MQ=255)

                                      cTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTgg                                                                                                  <  2:83958/140‑1 (MQ=255)

                                       ttCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTTAAGGCAGTTggg                                                                                                 <  2:786582/140‑1 (MQ=255)

                                       ttCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTggg                                                                                                 <  1:1007802/140‑1 (MQ=255)

                                       ttCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTggg                                                                                                 <  2:1752696/140‑1 (MQ=255)

                                       ttCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTggg                                                                                                 <  1:937474/140‑1 (MQ=255)

                                       ttCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTggg                                                                                                 <  1:1129825/140‑1 (MQ=255)

                                       ttCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTggg                                                                                                 <  1:1610547/140‑1 (MQ=255)

                                             cgcCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTgg                                                                                                  <  1:1372205/133‑1 (MQ=255)

                                             cgcCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTa                                                                                           <  1:780935/140‑1 (MQ=255)

                                              gcCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTaa                                                                                          <  1:63394/140‑1 (MQ=255)

                                              gcCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTaa                                                                                          <  2:1418907/140‑1 (MQ=255)

                                               ccGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAAc                                                                                         <  1:797099/140‑1 (MQ=255)

                                                 ggAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACag                                                                                       <  2:1840729/140‑1 (MQ=255)

                                                  gAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGtt                                                                                               >  2:170708/1‑131 (MQ=255)

                                                  gAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACagt                                                                                      >  1:1567938/1‑140 (MQ=255)

                                                    gTCACATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACagtag                                                                                    >  2:1636722/1‑140 (MQ=255)

                                                        cATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCa                                                                                <  1:595487/140‑1 (MQ=255)

                                                             tCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAgcgc                                                                           <  2:188235/140‑1 (MQ=255)

                                                             tCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAgcgc                                                                           <  1:1403913/140‑1 (MQ=255)

                                                                  aGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGACCCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGc                                                                      <  2:739974/140‑1 (MQ=255)

                                                                   gAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGcc                                                                     >  2:680855/1‑140 (MQ=255)

                                                                    aaaaaCCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCa                                                                    <  2:1774072/140‑1 (MQ=255)

                                                                      aaaCCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAAt                                                                  >  2:1009794/1‑140 (MQ=255)

                                                                         ccTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATAcc                                                               <  1:572829/140‑1 (MQ=255)

                                                                           tttGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGc                                                             >  1:755821/1‑140 (MQ=255)

                                                                           tttGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGc                                                             >  1:1498935/1‑140 (MQ=255)

                                                                            ttGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGcc                                                            >  2:937396/1‑140 (MQ=255)

                                                                                ttttCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCcagca                                                        <  1:1009794/140‑1 (MQ=255)

                                                                                     acaGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGcc                                                   <  2:225476/140‑1 (MQ=255)

                                                                                              gggATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGc                                          <  2:1734023/140‑1 (MQ=255)

                                                                                              gggATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGc                                          <  2:1325067/140‑1 (MQ=255)

                                                                                               ggATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCg                                         <  2:1603653/140‑1 (MQ=255)

                                                                                                 atatGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGAc                                       >  2:1171811/1‑140 (MQ=255)

                                                                                                 atatGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGAc                                       >  2:1571662/1‑140 (MQ=255)

                                                                                                   atGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACtt                                     >  2:1376351/1‑140 (MQ=255)

                                                                                                   atGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACtt                                     <  1:1679167/140‑1 (MQ=255)

                                                                                                   atGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACtt                                     >  2:523851/1‑140 (MQ=255)

                                                                                                     ggCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTc                                   >  2:1492396/1‑140 (MQ=255)

                                                                                                     ggCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTc                                   >  2:6638/1‑140 (MQ=255)

                                                                                                      gCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACttt                                    <  1:1278563/138‑1 (MQ=255)

                                                                                                      gCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACttt                                    >  2:1278563/1‑138 (MQ=255)

                                                                                                      gCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCa                                  >  1:266495/1‑140 (MQ=255)

                                                                                                      gCTCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCa                                  >  2:500978/1‑140 (MQ=255)

                                                                                                        tCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATc                                >  2:1528853/1‑140 (MQ=255)

                                                                                                        tCAGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATc                                >  2:2043990/1‑140 (MQ=255)

                                                                                                          aGATCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCt                              >  1:351737/1‑140 (MQ=255)

                                                                                                            aTCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTaa                            <  1:1492396/140‑1 (MQ=255)

                                                                                                             tCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTaaa                           <  1:1237140/140‑1 (MQ=255)

                                                                                                             tCGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTaaa                           <  1:2002679/140‑1 (MQ=255)

                                                                                                              cGACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGATTTTCATCCTAAAc                          <  1:1919272/140‑1 (MQ=255)

                                                                                                               gACAGCCACACCGGGAGTCACTGGAGAGATATCTTACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGGGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACt                         <  2:1542752/140‑1 (MQ=255)

                                                                                                               gACAGCCACACCGGGAGTCACTGGAGAGATATCATATAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACt                         <  1:1579114/140‑1 (MQ=255)

                                                                                                               gACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACt                         <  2:288021/140‑1 (MQ=255)

                                                                                                               gACAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACt                         <  1:6638/140‑1 (MQ=255)

                                                                                                                aCAGCCACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTc                                   >  2:1755897/1‑129 (MQ=255)

                                                                                                                    ccACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCtta                    <  1:680855/140‑1 (MQ=255)

                                                                                                                    ccACACCGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGGAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCtta                    >  1:1426326/1‑140 (MQ=255)

                                                                                                                         ccGGGAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATtata               <  1:1528853/140‑1 (MQ=255)

                                                                                                                             gAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGa           <  1:1758588/140‑1 (MQ=255)

                                                                                                                             gAGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGa           <  2:458309/140‑1 (MQ=255)

                                                                                                                              aGTCACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATTCCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGAt          <  1:1636722/140‑1 (MQ=255)

                                                                                                                                 cACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATaaa       <  1:845134/140‑1 (MQ=255)

                                                                                                                                 cACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATaaa       <  1:170760/140‑1 (MQ=255)

                                                                                                                                 cACTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATaaa       <  1:2043990/140‑1 (MQ=255)

                                                                                                                                  aCTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAAc      >  1:1298958/1‑140 (MQ=255)

                                                                                                                                  aCTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCCCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGa           <  2:220321/135‑1 (MQ=255)

                                                                                                                                  aCTGGAGAGATATCATACAGAGAGAGATCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGa           >  1:220321/1‑135 (MQ=255)

                                                                                                                                   cTGGAGAGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACt     <  2:746367/140‑1 (MQ=255)

                                                                                                                                      gagagaTATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAg  <  2:1240077/140‑1 (MQ=255)

                                                                                                                                      gagagaTATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAg  <  2:1781923/140‑1 (MQ=255)

ACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAG  >  NC_000913/3384026‑3384299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`