BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,438,365	C→A	A220E (GCG→GAG)	ytfI →	protein YtfI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,438,365	0	C	A	99.3%	497.6 / ‑5.7	141	A220E (GCG→GAG)	ytfI	protein YtfI
Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (71/69); total (71/70)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 144 total aligned reads displayed.

TATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAATTTTCTGGTTAATGCAGGTTGCCAACAAGTCGAATTAGCCGAACAAGTGGAT  >  NC_000913/4438228‑4438501

  taGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCtt                                                                                                                                      <  2:1569167/140‑1 (MQ=255)

  taGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCtt                                                                                                                                      >  2:49741/1‑140 (MQ=255)

  taGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCtt                                                                                                                                      <  1:1978334/140‑1 (MQ=255)

   aGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTa                                                                                                                                     >  1:1522583/1‑140 (MQ=255)

   aGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTa                                                                                                                                     <  1:1332878/140‑1 (MQ=255)

     tAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTaaa                                                                                                                                   >  1:1643737/1‑140 (MQ=255)

     tAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTaaa                                                                                                                                   <  1:1424490/140‑1 (MQ=255)

     tAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTaaa                                                                                                                                   <  1:293394/140‑1 (MQ=255)

      aaGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTaaaa                                                                                                                                  >  2:700556/1‑140 (MQ=255)

      aaGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTaaaa                                                                                                                                  >  1:251416/1‑140 (MQ=255)

       aGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAAt                                                                                                                                 >  1:1565380/1‑140 (MQ=255)

        gCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATc                                                                                                                                >  1:815680/1‑140 (MQ=255)

        gCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATc                                                                                                                                >  1:1133836/1‑140 (MQ=255)

        gCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATc                                                                                                                                >  2:1074380/1‑140 (MQ=255)

         cAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCt                                                                                                                               >  1:402079/1‑140 (MQ=255)

          aaaTTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTaa                                                                                                                                    >  1:1716418/1‑134 (MQ=255)

          aaaTTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTaa                                                                                                                                    <  2:1716418/134‑1 (MQ=255)

            aTTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAACTAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATg                                                                                                                            >  1:1071183/1‑140 (MQ=255)

            aTTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATg                                                                                                                            >  2:1580751/1‑140 (MQ=255)

            aTTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATg                                                                                                                            >  2:1836871/1‑140 (MQ=255)

             ttAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATgg                                                                                                                           >  1:92180/1‑140 (MQ=255)

              tAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGa                                                                                                                          <  1:388185/140‑1 (MQ=255)

              tAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGa                                                                                                                          <  2:1875365/140‑1 (MQ=255)

              tAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGa                                                                                                                          <  2:1384891/140‑1 (MQ=255)

              tAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCGGTGAGCTTAAGATCTATGGa                                                                                                                          <  2:1537624/140‑1 (MQ=255)

                       gTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGaa                                                                                                                 >  1:832965/1‑140 (MQ=255)

                         aaaTTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACg                                                                                                               <  1:655245/140‑1 (MQ=255)

                          aaTTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGt                                                                                                              <  2:1033916/140‑1 (MQ=255)

                           aTTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGtt                                                                                                             <  1:1232347/140‑1 (MQ=255)

                           aTTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGtt                                                                                                             >  1:580612/1‑140 (MQ=255)

                            tttGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGttt                                                                                                            <  2:1922951/140‑1 (MQ=255)

                             ttGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGtttt                                                                                                           <  1:285795/140‑1 (MQ=255)

                                aaCTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGt                                                                                                        >  2:671743/1‑140 (MQ=255)

                                aaCTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGt                                                                                                        >  2:777791/1‑140 (MQ=255)

                                aaCTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGt                                                                                                        >  1:1143027/1‑140 (MQ=255)

                                aaCTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGt                                                                                                        <  1:656482/140‑1 (MQ=255)

                                  cTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTaa                                                                                                      <  2:1566712/140‑1 (MQ=255)

                                     cAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAAt                                                                                                     >  2:1446985/1‑138 (MQ=255)

                                     cAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATgg                                                                                                   >  2:1364917/1‑140 (MQ=255)

                                     cAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATgg                                                                                                   <  2:1643737/140‑1 (MQ=255)

                                      aaaTACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGt                                                                                                  <  1:949697/140‑1 (MQ=255)

                                           ccAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCctc                                                                                             <  1:1040867/140‑1 (MQ=255)

                                             aggaggTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCtg                                                                                           <  1:162970/140‑1 (MQ=255)

                                             aggaggTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCtg                                                                                           <  2:251416/140‑1 (MQ=255)

                                               gaggTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCtgtt                                                                                         <  2:1110201/140‑1 (MQ=255)

                                               gaggTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCtgtt                                                                                         >  2:879680/1‑140 (MQ=255)

                                                   tGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGttt                                                                                     >  2:728619/1‑140 (MQ=255)

                                                    ggAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGtttt                                                                                    >  1:80756/1‑140 (MQ=255)

                                                    ggAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGtttt                                                                                    >  1:1044693/1‑140 (MQ=255)

                                                       aTTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTgga                                                                                 <  2:1993010/140‑1 (MQ=255)

                                                           aaGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGAt                                                                             >  2:1092838/1‑140 (MQ=255)

                                                              aaTGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGaa                                                                          <  2:166010/140‑1 (MQ=255)

                                                              aaTGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGaa                                                                          <  2:1390715/140‑1 (MQ=255)

                                                              aaTGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGaa                                                                          <  1:1104811/140‑1 (MQ=255)

                                                                tGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGa                                                                        >  2:1678612/1‑140 (MQ=255)

                                                                tGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGa                                                                        >  2:549539/1‑140 (MQ=255)

                                                                tGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGa                                                                        >  1:719847/1‑140 (MQ=255)

                                                                   gatggatgGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTa                                                                     <  1:49741/140‑1 (MQ=255)

                                                                     tggatggTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTAct                                                                   <  1:828995/140‑1 (MQ=255)

                                                                     tggatggTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTAct                                                                   <  1:671743/140‑1 (MQ=255)

                                                                        atggTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTActctc                                                                <  1:1433713/140‑1 (MQ=255)

                                                                        atggTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGCTTTGGAGGATGAAGATTActctc                                                                >  1:255356/1‑140 (MQ=255)

                                                                                    cGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAAtt                                                    >  2:934982/1‑140 (MQ=255)

                                                                                     gAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAAttt                                                   >  1:597884/1‑140 (MQ=255)

                                                                                     gAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAAttt                                                   >  1:974711/1‑140 (MQ=255)

                                                                                      aaaaaaTAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAAtttt                                                  <  1:879680/140‑1 (MQ=255)

                                                                                      aaaaaaTAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAAtttt                                                  <  1:439102/140‑1 (MQ=255)

                                                                                      aaaaaaTAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAAtttt                                                  <  1:1947476/140‑1 (MQ=255)

                                                                                      aaaaaaTAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAAtttt                                                  <  2:402079/140‑1 (MQ=255)

                                                                                      aaaaaaTAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAAtttt                                                  <  1:1500546/140‑1 (MQ=255)

                                                                                      aaaaaaTAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAAtttt                                                  >  1:1405026/1‑140 (MQ=255)

                                                                                      aaaaaaTAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAAtttt                                                  >  2:1500546/1‑140 (MQ=255)

                                                                                      aaaaaaTAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAAtttt                                                  >  1:1211419/1‑140 (MQ=255)

                                                                                      aaaaaaTAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAAtttt                                                  >  2:803384/1‑140 (MQ=255)

                                                                                            taatTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAATTTTCTGGtt                                            <  1:1678612/140‑1 (MQ=255)

                                                                                            taatTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAATTTTCTGGtt                                            <  1:1862488/140‑1 (MQ=255)

                                                                                                    gatAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAATTTTCTGGTTAATGCAgg                                    <  2:1405026/140‑1 (MQ=255)

                                                                                                    gatAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAATTTTCTGGTTAATGCAgg                                    >  1:1179070/1‑140 (MQ=255)

                                                                                                                   gTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAATTTTCTGGTTAATGCAGGTTGCCAACAAGTCGa                     <  2:1211419/140‑1 (MQ=255)

                                                                                                                   gTCATAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAATTTTCTGGTTAATGCAGGTTGCCAACAAGTCGa                     >  1:133519/1‑140 (MQ=255)

                                                                                                                      aTAGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAATTTTCTGGTTAATGCAGGTTGCCAACAAGTCGAAtt                  <  1:1015317/140‑1 (MQ=255)

                                                                                                                        aGAATCTTTGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAATTTTCTGGTTAATGCAGGTTGCCAACAAGTCGAATTAg                <  1:1364917/140‑1 (MQ=255)

                                                                                                                              tttGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAATTTTCTGGTTAATGCAGGTTGCCAACAAGTCGAATTAGCCGAAc          >  2:605239/1‑140 (MQ=255)

                                                                                                                              tttGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAATTTTCTGGTTAATGCAGGTTGCCAACAAGTCGAATTAGCCGAAc          >  2:133967/1‑140 (MQ=255)

                                                                                                                              tttGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAATTTTCTGGTTAATGCAGGTTGCCAACAAGTCGAATTAGCCGAAc          >  2:1160927/1‑140 (MQ=255)

                                                                                                                              tttGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAATTTTCTGGTTAATGCAGGTTGCCAACAAGTCGAATTAGCCGAAc          >  2:1014540/1‑140 (MQ=255)

                                                                                                                              tttGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAATTTTCTGGTTAATGCAGGTTGCCAACAAGTCGAATTAGCCGAAc          <  1:758897/140‑1 (MQ=255)

                                                                                                                              tttGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAATTTTCTGGTTAATGCAGGTTGCCAACAAGTCGAATTAGCCGAAc          >  2:1583424/1‑140 (MQ=255)

                                                                                                                              tttGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAATTTTCTGGTTAATGCAGGTTGCCAACAAGTCGAATTAGCCGAAc          >  2:2012449/1‑140 (MQ=255)

                                                                                                                              tttGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAATTTTCTGGTTAATGCAGGTTGCCAACAAGTCGAATTAGCCGAAc          >  1:622700/1‑140 (MQ=255)

                                                                                                                                tGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAATTTTCTGGTTAATGCAGGTTGCCAACAAGTCGAATTAGCCGAACaa        >  1:494202/1‑140 (MQ=255)

                                                                                                                                tGGGCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAATTTTCTGGTTAATGCAGGTTGCCAACAAGTCGAATTAGCCGAACaa        >  2:741999/1‑140 (MQ=255)

                                                                                                                                 gggCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAATTTTCTGGTTAATGCAGGTTGCCAACAAGTCGAATTAGCCGAACAAg       >  1:1586442/1‑140 (MQ=255)

                                                                                                                                  ggCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAATTTTCTGGTTAATGCAGGTTGCCAACAAGTCGAATTAGCCGAACAAGt      >  2:403723/1‑140 (MQ=255)

                                                                                                                                  ggCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAATTTTCTGGTTAATGCAGGTTGCCAACAAGTCGAATTAGCCGAACAAGt      <  2:584285/140‑1 (MQ=255)

                                                                                                                                  ggCTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAATTTTCTGGTTAATGCAGGTTGCCAACAAGTCGAATTAGCCGAACAAGt      <  1:852739/140‑1 (MQ=255)

                                                                                                                                    cTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAATTTTCTGGTTAATGCAGGTTGCCAACAAGTCGAATTAGCCGAACAAGTgg    <  1:1583424/140‑1 (MQ=255)

                                                                                                                                    cTTTGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAATTTTCTGGTTAATGCAGGTTGCCAACAAGTCGAATTAGCCGAACAAGTgg    <  1:611041/140‑1 (MQ=255)

                                                                                                                                      ttGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAATTTTCTGGTTAATGCAGGTTGCCAACAAGTCGAATTAGCCGAACAAGTGGAt  <  2:622700/140‑1 (MQ=255)

                                                                                                                                      ttGAGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAATTTTCTGGTTAATGCAGGTTGCCAACAAGTCGAATTAGCCGAACAAGTGGAt  <  2:1522583/140‑1 (MQ=255)

TATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAGGATGAAGATTACTCTCTTATTTATAATTTTCTGGTTAATGCAGGTTGCCAACAAGTCGAATTAGCCGAACAAGTGGAT  >  NC_000913/4438228‑4438501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`