Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	832,483	C→T	L156L (TTG→TTA)	ybiA ←	N‑glycosidase YbiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	832,483	0	C	T	100.0%	354.4 / NA	98	L156L (TTG→TTA)	ybiA	N‑glycosidase YbiA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (44/54);  total (44/54)

CTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGC  >  NC_000913/832348‑832618                                                                                                                                        |                                                                                                                                        cTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAAtt                                                                                                                                     >  1:435093/1‑140 (MQ=255)   tttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGt                                                                                                                                   <  1:1230107/140‑1 (MQ=255)     tGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTc                                                                                                                                 >  2:829055/1‑140 (MQ=255)        aGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTcgcg                                                                                                                              <  2:1038332/140‑1 (MQ=255)        aGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTcgcg                                                                                                                              <  1:1266611/140‑1 (MQ=255)        aGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTcgcg                                                                                                                              <  2:1922809/140‑1 (MQ=255)           ccGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGTGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCaa                                                                                                                           >  2:367665/1‑140 (MQ=255)           ccGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCaa                                                                                                                           <  2:226702/140‑1 (MQ=255)           ccGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCaa                                                                                                                           <  2:341962/140‑1 (MQ=255)           ccGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACC‑‑GACGTTACTTCTCTATAGCTAATTGTTCGCGCAACt                                                                                                                         >  2:143525/1‑140 (MQ=255)            cGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTaa                                                                                                                                       >  2:727501/1‑127 (MQ=255)            cGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAAc                                                                                                                          >  1:1757496/1‑140 (MQ=255)            cGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAAc                                                                                                                          >  1:929322/1‑140 (MQ=255)             ggATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACt                                                                                                                         <  2:1325290/140‑1 (MQ=255)             ggATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACt                                                                                                                         <  1:2048518/140‑1 (MQ=255)              gATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCaa                                                                                                                           >  2:1020279/1‑137 (MQ=255)              gATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCaa                                                                                                                           <  1:1020279/137‑1 (MQ=255)              gATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTc                                                                                                                        <  1:1711071/140‑1 (MQ=255)              gATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTc                                                                                                                        >  2:557941/1‑140 (MQ=255)              gATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTc                                                                                                                        <  2:733736/140‑1 (MQ=255)              gATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTc                                                                                                                        >  1:667215/1‑140 (MQ=255)                  aGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCAtt                                                                                                                    <  2:1503778/140‑1 (MQ=255)                   ggCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTa                                                                                                                   <  2:1370624/140‑1 (MQ=255)                   ggCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTa                                                                                                                   <  2:875852/140‑1 (MQ=255)                   ggCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTa                                                                                                                   <  1:490055/140‑1 (MQ=255)                        ttACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAgg                                                                                                              >  2:244586/1‑140 (MQ=255)                        ttACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAgg                                                                                                              <  2:405305/140‑1 (MQ=255)                         tACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGt                                                                                                             >  1:36658/1‑140 (MQ=255)                         tACGCCGCATCCGGCATTTCACAACAAGC‑CTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTa                                                                                                            >  2:650594/1‑140 (MQ=255)                           cgccgcATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAg                                                                                                           <  1:1311704/140‑1 (MQ=255)                              cgcATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGccc                                                                                                        >  1:1440938/1‑140 (MQ=255)                              cgcATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGc                                                                                                          >  2:210138/1‑138 (MQ=255)                              cgcATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGc                                                                                                          <  1:210138/138‑1 (MQ=255)                                cATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAg                                                                                                      >  2:830001/1‑140 (MQ=255)                                 acccGGCATTTCAGATCAACACCTTGTCACCAATTTTCGGTCACGGAAAAAAAATTATTTGTTATACTGCCCAGAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGt                                                                                                     <  2:508121/138‑1 (MQ=255)                                 aTCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGt                                                                                                     <  1:1966033/140‑1 (MQ=255)                                  tCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTc                                                                                                    <  2:36658/140‑1 (MQ=255)                                  tCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTc                                                                                                    <  2:1551898/140‑1 (MQ=255)                                      gCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGAtt                                                                                                <  1:892641/140‑1 (MQ=255)                                      gCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACC‑‑GACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCt                                                                                              <  1:143525/140‑1 (MQ=255)                                        aTTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCt                                                                                              <  2:237323/140‑1 (MQ=255)                                        aTTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCt                                                                                              <  2:65834/140‑1 (MQ=255)                                         tttCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCtt                                                                                             <  2:929322/140‑1 (MQ=255)                                            cacaACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGcc                                                                                          <  1:829055/140‑1 (MQ=255)                                            cacaACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGcc                                                                                          <  1:1716250/140‑1 (MQ=255)                                            cacaACAAGC‑CTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGccc                                                                                         <  1:650594/140‑1 (MQ=255)                                             acaacaAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGccc                                                                                         <  2:435093/140‑1 (MQ=255)                                                 caaGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTAc                                                                                     >  2:1923862/1‑140 (MQ=255)                                                  aaGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTAcc                                                                                    >  1:1490351/1‑140 (MQ=255)                                                        ttGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGacc                                                                              <  1:244586/140‑1 (MQ=255)                                                        ttGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGacc                                                                              <  2:1757496/140‑1 (MQ=255)                                                        ttGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGacc                                                                              <  1:1923862/140‑1 (MQ=255)                                                        ttGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGacc                                                                              <  1:1477954/140‑1 (MQ=255)                                                           tCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGaccacc                                                                           >  1:270520/1‑140 (MQ=255)                                                             aGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGt                                                                         <  1:563585/140‑1 (MQ=255)                                                               cAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGtct                                                                       <  2:1610932/140‑1 (MQ=255)                                                               cAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGtct                                                                       >  2:951566/1‑140 (MQ=255)                                                               cAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGtct                                                                       >  1:262490/1‑140 (MQ=255)                                                                  tttGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTccc                                                                    >  2:139120/1‑140 (MQ=255)                                                                  tttGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTccc                                                                    <  2:1573589/140‑1 (MQ=255)                                                                    tGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACAACCGTCTCCCCa                                                                  >  2:670011/1‑140 (MQ=255)                                                                       gaACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTa                                                               >  2:396897/1‑140 (MQ=255)                                                                        aaCACCGGAAAAAATTTATCTGTTTTGCCGCCCATAAGCCACCGAGACGTTACTTCTCGATAGCTAATTGGTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGTCCACCGTCTCCCCAGTaa                                                              <  2:550830/140‑1 (MQ=255)                                                                           acGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCa                                                           <  2:1490351/140‑1 (MQ=255)                                                                            cGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCAt                                                          >  2:1258972/1‑140 (MQ=255)                                                                               gAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGt                                                       <  1:498751/140‑1 (MQ=255)                                                                                     aTTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCg                                                 <  1:882043/140‑1 (MQ=255)                                                                                                ttACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGt                                                       >  1:1210656/1‑123 (MQ=255)                                                                                                ttACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGt                                                       <  2:1210656/123‑1 (MQ=255)                                                                                                ttACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAAcc                                      >  2:1260222/1‑140 (MQ=255)                                                                                                ttACCGCCCATAAGCCACCGAGACGTTAC‑TCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCa                                     >  1:1187117/1‑140 (MQ=255)                                                                                                      cccATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCg                                                 >  1:631170/1‑123 (MQ=255)                                                                                                      cccATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCg                                                 <  2:631170/123‑1 (MQ=255)                                                                                                       ccATAAGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTg                               >  2:1869462/1‑140 (MQ=255)                                                                                                           aaGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCggg                           >  1:489893/1‑140 (MQ=255)                                                                                                            aGCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGgc                          <  1:1600462/140‑1 (MQ=255)                                                                                                             gCCACCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGgcg                         <  1:452183/140‑1 (MQ=255)                                                                                                                aCCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCGCCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCgg                      <  1:670011/140‑1 (MQ=255)                                                                                                                aCCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCgg                      >  2:1110002/1‑140 (MQ=255)                                                                                                                aCCGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCgg                      <  1:139120/140‑1 (MQ=255)                                                                                                                 ccGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGt                     >  1:926268/1‑140 (MQ=255)                                                                                                                 ccGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGt                     >  1:1258571/1‑140 (MQ=255)                                                                                                                  cGAGACGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGtt                    <  2:262490/140‑1 (MQ=255)                                                                                                                    agaCGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGc                  <  1:951566/140‑1 (MQ=255)                                                                                                                     gaCGTTACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGcc                 >  1:437479/1‑140 (MQ=255)                                                                                                                         ttACTTCTCTATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCa             >  2:541746/1‑140 (MQ=255)                                                                                                                               ctctATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAgcg       >  1:403127/1‑140 (MQ=255)                                                                                                                                 ctATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGcgca     <  2:437479/140‑1 (MQ=255)                                                                                                                                 ctATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGcgca     <  2:926268/140‑1 (MQ=255)                                                                                                                                 ctATAGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGcgca     <  1:1258972/140‑1 (MQ=255)                                                                                                                                  tataGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGcgcac    >  2:1356490/1‑140 (MQ=255)                                                                                                                                  tataGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGcgcac    >  1:1206293/1‑140 (MQ=255)                                                                                                                                  tataGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGcgcac    >  2:1197255/1‑140 (MQ=255)                                                                                                                                  tataGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGcgcac    >  1:1633565/1‑140 (MQ=255)                                                                                                                                  tataGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGcgcac    <  2:403127/140‑1 (MQ=255)                                                                                                                                   ataGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGcgcacg   >  2:1302727/1‑140 (MQ=255)                                                                                                                                   ataGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGcgcacg   <  1:985278/140‑1 (MQ=255)                                                                                                                                    taGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGcgcacgc  >  1:1721962/1‑140 (MQ=255)                                                                                                                                    taGCTAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGcgcacgc  >  1:204443/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        CTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGC  >  NC_000913/832348‑832618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑