Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,611,955	C→A	A19E (GCG→GAG)	hyfR →	DNA‑binding transcriptional activator HyfR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,611,955	0	C	A	100.0%	99.4 / NA	29	A19E (GCG→GAG)	hyfR	DNA‑binding transcriptional activator HyfR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (20/9);  total (20/9)

GAACCGGCCATCTATCTGATGGCCAGAAAAATTGCGTGAGAAGGATTTCTCATTAATAAGGACTGTTGATGGCTATGTCAGACGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTA  >  NC_000913/2611832‑2612090                                                                                                                            |                                                                                                                                        gAACCGGCCATCTATCTGATGGCCAGAAAAATTGCGTGAGAAGGATTTCTCATTAATAAGGACTGTTGATGGCTATGTCAGACGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTc                                                                                                                         >  1:265561/1‑140 (MQ=255)           tctatctGATGGCCAGAAAAATTGCGTGAGAAGGATTTCTCATTAATAAGGACTGTTGATGGCTATGTCAGACGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGGAGAGGTAAACGGAATGCTCGCGGGGCGGt                                                                                                               >  1:178680/1‑140 (MQ=255)            ttatcgGAGGGCCGAAAAATTTGGGGGAGAAGGTTTTCTCATAAATAAGGATTGTTGATGGCTATGGCGGAGGGGGCGATGTTTCCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGtt                                                                                                              <  2:130018/139‑1 (MQ=255)                ctGATGGCCAGAAAAATTGCGTGAGAAGGATTTCTCATTAATAAGGACTGTTGATGGCTATGTCAGACGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGca                                                                                                          >  1:362666/1‑140 (MQ=255)                      gCCAGAAAAATTGCGTGAGAAGGATTTCTCATTAATAAGGACTGTTGATGGCTATGTCAGACGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGaaa                                                                                                    >  2:753737/1‑140 (MQ=255)                      gCCAGAAAAATTGCGTGAGAAGGATTTCTCATTAATAAGGACTGTTGATGGCTATGTCAGACGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGaaa                                                                                                    >  1:738894/1‑140 (MQ=255)                       ccAGAAAAATTGCGTGAGAAGGATTTCTCATTAATAAGGACTGTTGATGGCTATGTCAGACGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAac                                                                                                   >  2:869845/1‑140 (MQ=255)                       ccAGAAAAATTGCGTGAGAAGGATTTCTCATTAATAAGGACTGTTGATGGCTATGTCAGACGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAac                                                                                                   >  2:730987/1‑140 (MQ=255)                                      gagaGGGTTTTTTAATAAAAAGGGATTGTTGATGGTTATGTCAGAGGGGGCGTTGTTTCCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCtt                                                                                    <  2:873105/140‑1 (MQ=255)                                             ttttCTCATAAATAAGGACTGTTGATGGTTATGTCAGACGGGGCGTTTTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTAcg                                                                             <  1:114984/139‑1 (MQ=255)                                             ttttCTAATAAAAAAGGATTGTTGATGGCTATGTCAGAGGGGGGGTTTTTTCCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTAcg                                                                             <  1:806031/139‑1 (MQ=255)                                                           aGGACTGTTGATGGCTATGTCAGACGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGc                                                               >  1:658977/1‑140 (MQ=255)                                                                tGTTGATGGCTATGTCAGACGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTgccg                                                          >  1:408820/1‑140 (MQ=255)                                                                tGTTGATGGCTATGTCAGACGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTgccg                                                          >  1:350182/1‑140 (MQ=255)                                                                tGTTGATGGCTATGTCAGACGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTgccg                                                          >  2:25059/1‑140 (MQ=255)                                                                   tGATGGTTATGAAAGAGGGGGCGTTTTTCCCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTgccgccg                                                       <  2:52279/140‑1 (MQ=255)                                                                       ggCTATGTCAGACGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGt                                                   >  2:785073/1‑140 (MQ=255)                                                                            tGTCAGACGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGc                                              >  2:746843/1‑140 (MQ=255)                                                                            tGTCAGACGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGc                                              >  2:639122/1‑140 (MQ=255)                                                                             gTCAGAGGGGGCTTTTTTTCCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGcc                                             <  1:661643/140‑1 (MQ=255)                                                                             gTCAGACGGGGGGTTTTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGcc                                             <  1:443785/140‑1 (MQ=255)                                                                              tCAGACGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGAACCGGGTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTCCAGccc                                            >  2:229250/1‑140 (MQ=255)                                                                               cAGACGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCg                                           >  1:384014/1‑140 (MQ=255)                                                                                  aCGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTa                                        >  1:82605/1‑140 (MQ=255)                                                                                   cGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGGAc                                       >  1:843732/1‑140 (MQ=255)                                                                                      ggCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAAc                                    >  1:24904/1‑140 (MQ=255)                                                                                                     gCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATg                     <  2:362666/140‑1 (MQ=255)                                                                                                                   cAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAAc       <  2:820473/140‑1 (MQ=255)                                                                                                                        gAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTa  >  2:550892/1‑140 (MQ=255)                                                                                                                        gAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCCCAAAAGCAACTTCTa  >  1:351678/1‑140 (MQ=255)                                                                                                                            |                                                                                                                                        GAACCGGCCATCTATCTGATGGCCAGAAAAATTGCGTGAGAAGGATTTCTCATTAATAAGGACTGTTGATGGCTATGTCAGACGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTA  >  NC_000913/2611832‑2612090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑