Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,974,644	G→T	T457T (ACC→ACA)	aas ←	acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,974,644	0	G	T	100.0%	183.4 / NA	52	T457T (ACC→ACA)	aas	acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (24/28);  total (24/28)

ATCCTGCCAAAGCTGTTTGGTACTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACGGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACG  >  NC_000913/2974512‑2974780                                                                                                                                     |                                                                                                                                         aTCCTGCCAAAGCTGTTTGGTACTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAAc                                                                                                                                   <  1:61676/140‑1 (MQ=255)  tCCTGCCAAAGCTGTTTGGTACTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACt                                                                                                                                  >  2:604881/1‑140 (MQ=255)       ccAAAGCTGTTTGGTACTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGt                                                                                                                             <  2:833771/140‑1 (MQ=255)               gTTTGGTACTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACAcc                                                                                                                     <  1:183859/140‑1 (MQ=255)               gTTTGGTACTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACAcc                                                                                                                     <  2:270563/140‑1 (MQ=255)                tttGGTACTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCa                                                                                                                    >  1:194748/1‑140 (MQ=255)                tttGGTACTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCa                                                                                                                    >  2:602379/1‑140 (MQ=255)                     tACTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTcc                                                                                                               >  1:866767/1‑140 (MQ=255)                         ttCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGca                                                                                                           <  1:281826/140‑1 (MQ=255)                          tCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGcac                                                                                                          >  1:101963/1‑140 (MQ=255)                          tCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGcac                                                                                                          >  1:558687/1‑140 (MQ=255)                            ttGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACaa                                                                                                        <  2:101963/140‑1 (MQ=255)                            ttGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACaa                                                                                                        <  2:647077/140‑1 (MQ=255)                                         gcgcCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAg                                                                                           <  1:116207/140‑1 (MQ=255)                                              tGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGc                                                                                      <  2:812506/140‑1 (MQ=255)                                              tGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGc                                                                                      <  2:194748/140‑1 (MQ=255)                                              tGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGc                                                                                      <  2:866767/140‑1 (MQ=255)                                                   ccacATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGa                                                                                 <  2:658153/140‑1 (MQ=255)                                                         aGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAgg                                                                           >  1:71818/1‑140 (MQ=255)                                                          gCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGa                                                                          >  2:32654/1‑140 (MQ=255)                                                           cGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGTGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGaa                                                                         >  1:80684/1‑140 (MQ=255)                                                           cGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAg                                                                                           >  2:54530/1‑122 (MQ=255)                                                           cGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAg                                                                                           <  1:54530/122‑1 (MQ=255)                                                           cGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGaa                                                                         >  2:640562/1‑140 (MQ=255)                                                           cGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGaa                                                                         <  1:84011/140‑1 (MQ=255)                                                           cGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGaa                                                                         <  1:32654/140‑1 (MQ=255)                                                           cGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGaa                                                                         >  2:134987/1‑140 (MQ=255)                                                              aGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAacac                                                                      >  1:251683/1‑140 (MQ=255)                                                                   aTAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTg                                                                 <  1:604881/140‑1 (MQ=255)                                                                   aTAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTg                                                                 <  1:542995/140‑1 (MQ=255)                                                                     aGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAacac                                                                      <  1:290724/133‑1 (MQ=255)                                                                     aGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAacac                                                                      >  2:290724/1‑133 (MQ=255)                                                                     aGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCa                                                               >  2:238084/1‑140 (MQ=255)                                                                          tCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGt                                                          <  1:238084/140‑1 (MQ=255)                                                                           cATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTa                                                         >  2:546037/1‑140 (MQ=255)                                                                            aTACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTaa                                                        >  1:660994/1‑140 (MQ=255)                                                                               cGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGTGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCa                                                     <  2:80684/140‑1 (MQ=255)                                                                               cGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCa                                                     <  1:110348/140‑1 (MQ=255)                                                                                gggTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAg                                                    >  1:220793/1‑140 (MQ=255)                                                                                     ggCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCg                                               >  1:423897/1‑140 (MQ=255)                                                                                       cGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTa                                             >  2:759572/1‑140 (MQ=255)                                                                                              cgcgTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGc                                      <  2:618413/140‑1 (MQ=255)                                                                                                     tGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGt                               <  2:558687/140‑1 (MQ=255)                                                                                                     tGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGt                               <  1:495418/140‑1 (MQ=255)                                                                                                     tGACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGt                               >  2:99104/1‑140 (MQ=255)                                                                                                      gACCGAGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTc                              >  2:245990/1‑140 (MQ=255)                                                                                                           aGGAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGccc                         >  1:760945/1‑140 (MQ=255)                                                                                                             gAAAGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCaa                       <  2:337662/140‑1 (MQ=255)                                                                                                               aaGTCGAGGTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAg                     <  1:28573/140‑1 (MQ=255)                                                                                                                      ggTGCCGAACAACACTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGaa              <  2:71818/140‑1 (MQ=255)                                                                                                                              acaacaCTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGt      <  1:245990/140‑1 (MQ=255)                                                                                                                                  cacTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACg  >  1:99429/1‑140 (MQ=255)                                                                                                                                  cacTGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACg  >  1:302782/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                         ATCCTGCCAAAGCTGTTTGGTACTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACGGTGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACG  >  NC_000913/2974512‑2974780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑