Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,232,125	C→A	A154E (GCA→GAA)	fadH →	2,4‑dienoyl‑CoA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,232,125	0	C	A	100.0%	155.8 / NA	44	A154E (GCA→GAA)	fadH	2,4‑dienoyl‑CoA reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (19/25);  total (19/25)

ACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAG  >  NC_000913/3231993‑3232259                                                                                                                                     |                                                                                                                                       aCCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATac                                                                                                                                 >  1:584762/1‑140 (MQ=255)   cAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGTGGGAGGAAGGATacga                                                                                                                               <  1:300209/140‑1 (MQ=255)   cAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATacga                                                                                                                               <  2:832550/140‑1 (MQ=255)   cAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATacga                                                                                                                               <  2:584762/140‑1 (MQ=255)   cAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATacga                                                                                                                               <  1:280431/140‑1 (MQ=255)   cAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATacga                                                                                                                               <  2:150982/140‑1 (MQ=255)   cAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATacga                                                                                                                               <  2:131223/140‑1 (MQ=255)   cAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATacga                                                                                                                               <  1:737992/140‑1 (MQ=255)   cAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATacga                                                                                                                               <  1:579798/140‑1 (MQ=255)    aaCCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGCGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATacgac                                                                                                                              >  2:732978/1‑140 (MQ=255)    aaCCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATacgac                                                                                                                              >  2:18480/1‑140 (MQ=255)                tCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTg                                                                                                                  >  1:584179/1‑140 (MQ=255)                    cccGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATgg                                                                                                              <  2:586141/140‑1 (MQ=255)                    cccGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATgg                                                                                                              >  1:574381/1‑140 (MQ=255)                         ccGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTcc                                                                                                         >  2:896661/1‑140 (MQ=255)                           gCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGa                                                                                                       <  2:557763/140‑1 (MQ=255)                           gCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCGGCAACTGATCGACAATTTCGCCCGCTGCGCGGAACTGGCGCGGGAGGAAGGATGCGACGGTGTAGAGGTGATGGGTTCCGa                                                                                                       <  2:479427/140‑1 (MQ=255)                            cATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGaa                                                                                                      <  1:623446/140‑1 (MQ=255)                            cATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGaa                                                                                                      <  2:503246/140‑1 (MQ=255)                            cATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGaa                                                                                                      <  2:58210/140‑1 (MQ=255)                            cATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGaa                                                                                                      <  1:18888/140‑1 (MQ=255)                            cATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGaa                                                                                                      <  1:895569/140‑1 (MQ=255)                                 cAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTa                                                                                                 >  2:415701/1‑140 (MQ=255)                                         cATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCa                                                                                         >  1:510784/1‑140 (MQ=255)                                           tCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAAc                                                                                       >  1:132615/1‑140 (MQ=255)                                              aCCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGaa                                                                                    >  1:666137/1‑140 (MQ=255)                                                  tttCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTc                                                                                >  2:505870/1‑140 (MQ=255)                                                       tGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACg                                                                           <  2:311102/140‑1 (MQ=255)                                                       tGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACg                                                                           >  2:850958/1‑140 (MQ=255)                                                             aTGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTgcgc                                                                     >  1:478775/1‑140 (MQ=255)                                                              tGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCa                                                                    <  1:269278/140‑1 (MQ=255)                                                                   tAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAAt                                                               <  2:666137/140‑1 (MQ=255)                                                                     aGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCa                                                             <  2:584179/140‑1 (MQ=255)                                                                     aGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCa                                                             <  2:478775/140‑1 (MQ=255)                                                                     aGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCa                                                             <  2:455327/140‑1 (MQ=255)                                                                          tGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTa                                                        >  2:276730/1‑140 (MQ=255)                                                                               agaTCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGAc                                                   <  1:115096/140‑1 (MQ=255)                                                                                 aTCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCa                                                 >  1:523698/1‑140 (MQ=255)                                                                                         aCTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGggcg                                         >  1:486714/1‑140 (MQ=255)                                                                                             aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGa                                     >  2:70380/1‑140 (MQ=255)                                                                                                              cTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGT‑GGGCGGCGATTACCGCAACCGGATGCg                   >  2:221871/1‑140 (MQ=255)                                                                                                                     cAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGc             <  1:18480/140‑1 (MQ=255)                                                                                                                       aCTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCg           <  2:574381/140‑1 (MQ=255)                                                                                                                       aCTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCg           <  1:732978/140‑1 (MQ=255)                                                                                                                         tGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTa         >  2:395495/1‑140 (MQ=255)                                                                                                                                ggaggaAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAagtag  >  1:5244/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                       ACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAG  >  NC_000913/3231993‑3232259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑