Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,092,290	G→T	A26D (GCC→GAC)	frvB ←	putative PTS enzyme IIBC component FrvB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,092,290	0	G	T	100.0%	130.8 / NA	38	A26D (GCC→GAC)	frvB	putative PTS enzyme IIBC component FrvB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (18/20);  total (18/20)

TCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGC  >  NC_000913/4092153‑4092426                                                                                                                                          |                                                                                                                                         tCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCt                                                                                                                                        >  2:888724/1‑140 (MQ=255) tCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCt                                                                                                                                        >  2:714193/1‑140 (MQ=255)     gCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCg                                                                                                                                    >  2:891896/1‑140 (MQ=255)     gCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCg                                                                                                                                    >  1:891822/1‑140 (MQ=255)     gCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCCGTTCCAGGTCTTCCg                                                                                                                                    >  1:409914/1‑140 (MQ=255)      ccACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGc                                                                                                                                   <  2:873164/140‑1 (MQ=255)      ccACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCCGTTCCAGGTCTTCCGc                                                                                                                                   <  2:409914/140‑1 (MQ=255)             cGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATg                                                                                                                            <  1:596516/140‑1 (MQ=255)              ggTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGt                                                                                                                           <  1:891896/140‑1 (MQ=255)               gTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTa                                                                                                                          >  2:147916/1‑140 (MQ=255)               gTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTa                                                                                                                          >  2:432153/1‑140 (MQ=255)               gTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTa                                                                                                                          >  2:636504/1‑140 (MQ=255)                            cGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATc                                                                                                             >  1:872006/1‑140 (MQ=255)                                  cGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCcggcg                                                                                                       <  2:227425/140‑1 (MQ=255)                                     cAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCgggg                                                                                                    <  2:351558/140‑1 (MQ=255)                                     cAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCgggg                                                                                                    >  2:524147/1‑140 (MQ=255)                                     cAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCgggg                                                                                                    <  1:195481/140‑1 (MQ=255)                                     cAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCgggg                                                                                                    <  1:147916/140‑1 (MQ=255)                                               ctctTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGt                                                                                            >  2:84686/1‑138 (MQ=255)                                               ctctTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGt                                                                                            <  1:84686/138‑1 (MQ=255)                                                       tGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACa                                                                                  <  2:379124/140‑1 (MQ=255)                                                       tGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACa                                                                                  <  1:432153/140‑1 (MQ=255)                                                       tGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACa                                                                                  <  2:428802/140‑1 (MQ=255)                                                        ggATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACaa                                                                                 <  2:373169/140‑1 (MQ=255)                                                           tAAGCTGTTTTCGACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTGTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATAc                                                                              <  2:734483/140‑1 (MQ=255)                                                                                  aCCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACt                                                       <  1:714193/140‑1 (MQ=255)                                                                                      ttGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCt                                                   >  2:372629/1‑140 (MQ=255)                                                                                       tGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCtt                                                  >  2:495410/1‑140 (MQ=255)                                                                                        gAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTa                                                 <  2:891822/140‑1 (MQ=255)                                                                                             tCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCCCTTCCTTATGAGc                                            >  1:893252/1‑140 (MQ=255)                                                                                              ccACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCt                                           <  1:223642/140‑1 (MQ=255)                                                                                                    ttATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCCTATGAGCTGAGGGt                                     <  1:636504/140‑1 (MQ=255)                                                                                                                   gagaACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGc                      >  1:861241/1‑140 (MQ=255)                                                                                                                    agaACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCa                     >  2:198525/1‑140 (MQ=255)                                                                                                                         gggCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCAc                >  1:877060/1‑140 (MQ=255)                                                                                                                              ttCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGt           <  2:659916/140‑1 (MQ=255)                                                                                                                              ttCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGt           >  2:388351/1‑140 (MQ=255)                                                                                                                              ttCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGt           <  1:198525/140‑1 (MQ=255)                                                                                                                                    tCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTAt     <  2:669320/140‑1 (MQ=255)                                                                                                                                       aGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGc  <  2:872006/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         TCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGC  >  NC_000913/4092153‑4092426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑