Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,359,925	G→T	N328K (AAC→AAA)	puuA ←	glutamate‑putrescine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,359,925	0	G	T	100.0%	165.2 / NA	47	N328K (AAC→AAA)	puuA	glutamate‑putrescine ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (22/25);  total (22/25)

CCGCCACGCGATATTCCACGCGGTGATTATGACGGTCGCCGCACGGAATACGCAGGGCGACGGTGCGGTTGTTATGGCCCCACGACGCCTGCGTCGGCACATACATTCCCGGCTGGAAGCGGCGATACGAGTTCACGTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTTTGCATACTGA  >  NC_000913/1359789‑1360055                                                                                                                                         |                                                                                                                                   ccGCCACGCGATATTCCACGCGGTGATTATGACGGTCGCCGCACGGAATACGCAGGGCGACGGTGCGGTTGTTATGGCCCCACGACGCCTGCGTCGGCACATACATTCCCGGCTGGAAGCGGCGATACGAGTTCACtttt                                                                                                                                 >  1:422355/1‑140 (MQ=255) ccGCCACGCGATATTCCACGCGGTGATTATGACGGTCGCCGCACGGAATACGCAGGGCGACGGTGCGGTTGTTATGGCCCCACGACGCCTGCGTCGGCACATACATTCCCGGCTGGAAGCGGCGATACGAGTTCACtttt                                                                                                                                 >  1:357147/1‑140 (MQ=255)        gcgATATTCCACGCGGTGATTATGACGGTCGCCGCACGGAATACGCAGGGCGACGGTGCGGTTGTTATGGCCCCACGACGCCTGCGTCGGCACATACATTCCCGGCTGGAAGCGGCGATACGAGTTCACTTTTGGTGCCa                                                                                                                          >  1:105729/1‑140 (MQ=255)                      ggTGATTATGACGGTCGCCGCACGGAATACGCAGGGCGACGGTGCGGTTGTTATGGCCCCACGACGCCTGCGTCGGCACATACATTCCCGGCTGGAAGCGGCGATACGAGTTCACTTTTGGTGCCAGCAACGCCATcgac                                                                                                            >  2:214219/1‑140 (MQ=255)                         gATTATGACGGTCGCCGCACGGAATACGCAGGGCGACGGTGCGGTTGTTATGGCCCCACGACGCCTGCGTCGGCACATACATTCCCGGCTGGAAGCGGCGATACGAGTTCACTTTTGGTGCCAGCAACGCCATCgacgac                                                                                                         >  2:624398/1‑140 (MQ=255)                            tATGACGGTCGCCGCACGGAATACGCAGGGCGACGGTGCGGTTGTTATGGCCCCACGACGCCTGCGTCGGCACATACATTCCCGGCTGGAAGCGGCGATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGc                                                                                                      <  1:261906/140‑1 (MQ=255)                              tGACGGTCGCCGCACGGAATACGCAGGGCGACGGTGCGGTTGTTATGGCCCCACGACGCCTGCGTCGGCACATACATTCCCGGCTGGAAGCGGCGATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCAt                                                                                                    <  1:711280/140‑1 (MQ=255)                              tGACGGTCGCCGCACGGAATACGCAGGGCGACGGTGCGGTTGTTATGGCCCCACGACGCCTGCGTCGGCACATACATTCCCGGCTGGAAGCGGCGATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCAt                                                                                                    <  2:779454/140‑1 (MQ=255)                                aCGGTCGCCGCACGGAATACGCAGGGCGACGGTGCGGTTGTTATGGCCCCACGACGCCTGCGTCGGCACATACATTCCCGGCTGGAAGCGGCGATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCa                                                                                                  <  2:467792/140‑1 (MQ=255)                                 cGGTCGCCGCACGGAATACGCAGGGCGACGGTGCGGTTGTTATGGCCCCACGACGCCTGCGTCGGCACATACATTCCCGGCTGGAAGCGGCGATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAg                                                                                                 >  2:710592/1‑140 (MQ=255)                                 cGGTCGCCGCACGGAATACGCAGGGCGACGGTGCGGTTGTTATGGCCCCACGACGCCTGCGTCGGCACATACATTCCCGGCTGGAAGCGGCGATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAg                                                                                                 >  2:584403/1‑140 (MQ=255)                                 cGGTCGCCGCACGGAATACGCAGAGCGACGGTGCGGTTGTTATGGCCCCACGACGCCTGCGTCGGCACATACATTCCCGGCTGGAAGCGGCGATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAg                                                                                                 <  2:288246/140‑1 (MQ=255)                                    tCGCCGCACGGAATACGCAGGGCGACGGTGCGGTTGTTATGGCCCCACGACGCCTGCGTCGGCACATACATTCCCGGCTGGAAGCGGCGATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTc                                                                                              >  1:309042/1‑140 (MQ=255)                                    tCGCCGCACGGAATACGCAGGGCGACGGTGCGGTTGTTATGGCCCCACGACGCCTGCGTCGGCACATACATTCCCGGCTGGAAGCGGCGATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTc                                                                                              >  2:358852/1‑140 (MQ=255)                                                        ggCGACGGTGCGGTTGTTATGGCCCCACGACGCCTGCGTCGGCACATACATTCCCGGCTGGAAGCGGCGATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCt                                                                          >  2:116036/1‑140 (MQ=255)                                                         gCGACGGTGCGGTTGTTATGGCCCCACGACGCCTGCGTCGGCACATACATTCCCGGCTGGAAGCGGCGATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCtt                                                                         >  1:46278/1‑140 (MQ=255)                                                         gCGACGGTGCGGTTGTTATGGCCCCACGACGCCTGCGTCGGCACATACATTCCCGGCTGGAAGCGGCGATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCtt                                                                         >  1:682600/1‑140 (MQ=255)                                                                tGCGGTTGTTATGGCCCCACGACGCCTGCGTCGGCACATACATTCCCGGCTGGAAGCGGCGATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTcagc                                                                  <  2:522256/140‑1 (MQ=255)                                                                  cGGTTGTTATGGCCCCACGACGCCTGCGTCGGCACATACATTCCCGGCTGGAAGCGGCGATACGAGTTCACTTTTGGTGCCAGCCACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTcagcag                                                                >  2:661985/1‑140 (MQ=255)                                                                  cGGTTGTTATGGCCCCACGACGCCTGCGTCGGCACATACATTCCCGGCTGGAAGCGGCGATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCGATCATCCCGGCGAGCATCTTTTTcagcag                                                                >  2:549/1‑140 (MQ=255)                                                                          aTGGCCCCACGACGCCTGCGTCGGCACATACATTCCCGGCTGGAAGCGGCGATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAAt                                                        >  1:418330/1‑140 (MQ=255)                                                                          aTGGCCCCACGACGCCTGCGTCGGCACATACATTCCCGGCTGGAAGCGGCGATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAAt                                                        >  1:649644/1‑140 (MQ=255)                                                                              ccccACGACGCCTGCGTCGGCACATACATTCCCGGCTGGAAGCGGCGATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTc                                                    >  2:738300/1‑140 (MQ=255)                                                                               cccACGACGCCTGCGTCGGCACATACATTCCCGGCTGGAAGCGGCGATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCg                                                   >  1:578107/1‑140 (MQ=255)                                                                                       gCCTGCGTCGGCACATACATTCCCGGCTGGAAGCGGCGATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGGCGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCcgc                                           <  1:499910/140‑1 (MQ=255)                                                                                           gCGTCGGCACATACATTCCCGGCTGGAAGCGGCGATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCa                                       <  1:24552/140‑1 (MQ=255)                                                                                                 gCACATACATTCCCGGCTGGAAGCGGCGATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGc                                 <  2:593544/140‑1 (MQ=255)                                                                                                       acatTCCCGGCTGGAAGCGGCGATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTtc                           <  1:214219/140‑1 (MQ=255)                                                                                                             ccGGCTGGAAGCGGCGATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCa                     <  1:599675/140‑1 (MQ=255)                                                                                                              cGGCTGGAAGCGGCGATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCAc                    <  2:357147/140‑1 (MQ=255)                                                                                                               ggCTGGAAGCGGCGATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACg                   >  1:187103/1‑140 (MQ=255)                                                                                                               ggCTGGAAGCGGCGATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACg                   >  2:88584/1‑140 (MQ=255)                                                                                                               ggCTGGAAGCGGCGATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACg                   >  1:407143/1‑140 (MQ=255)                                                                                                                gCTGGAAGCGGCGATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGa                  >  1:544129/1‑140 (MQ=255)                                                                                                                gCTGGAAGCGGCGATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGa                  >  2:494986/1‑140 (MQ=255)                                                                                                                gCTGGAAGCGGCGATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGa                  >  1:579692/1‑140 (MQ=255)                                                                                                                       gcggcgATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTAtttt           <  2:669662/140‑1 (MQ=255)                                                                                                                          gcgATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTTTGCa        <  2:105729/140‑1 (MQ=255)                                                                                                                          gcgATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTTTGCa        <  1:584403/140‑1 (MQ=255)                                                                                                                          gcgATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTTTGCa        <  1:710592/140‑1 (MQ=255)                                                                                                                           cgATACGGGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTTTGCAt       <  2:105100/140‑1 (MQ=255)                                                                                                                           cgATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCGATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTTTGCAt       <  1:549/140‑1 (MQ=255)                                                                                                                            gATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTTTGCATa      <  2:579692/140‑1 (MQ=255)                                                                                                                            gATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTTTGCATa      <  1:793700/140‑1 (MQ=255)                                                                                                                            gATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTTTGCATa      <  1:381784/140‑1 (MQ=255)                                                                                                                            gATACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTTTGCATa      <  2:833174/140‑1 (MQ=255)                                                                                                                             aTACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTTTGCATAc     <  1:868090/140‑1 (MQ=255)                                                                                                                             aTACGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTTTGCATAc     <  1:318678/140‑1 (MQ=255)                                                                                                                                cGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTTTGCATACTga  <  2:578107/140‑1 (MQ=255)                                                                                                                                cGAGTTCACTTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTTTGCATACTga  <  2:309042/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                   CCGCCACGCGATATTCCACGCGGTGATTATGACGGTCGCCGCACGGAATACGCAGGGCGACGGTGCGGTTGTTATGGCCCCACGACGCCTGCGTCGGCACATACATTCCCGGCTGGAAGCGGCGATACGAGTTCACGTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTTTGCATACTGA  >  NC_000913/1359789‑1360055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑