BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	987,174	(A)_6→5	intergenic (‑192/+411)	ompF ← / ← asnS	outer membrane porin F/asparagine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	987,169	0	A	.	100.0%	626.4 / NA	149	intergenic (‑187/+416)	ompF/asnS	outer membrane porin F/asparagine‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (72/77); total (72/77)

Only 100 of 154 total aligned reads displayed.

ATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATCTATTTATATGATTTCCTTATATTTAAATTAACTAAACGGA  >  NC_000913/987031‑987301

aTAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGaa                                                                                                                                     <  1:1626793/140‑1 (MQ=255)

  aaGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGaaaa                                                                                                                                   <  2:1658686/140‑1 (MQ=255)

         gTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTa                                                                                                                           >  1:750954/1‑140 (MQ=255)

         gTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTa                                                                                                                           >  2:29256/1‑140 (MQ=255)

          tCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGaaaa                                                                                                                                   >  2:443932/1‑132 (MQ=255)

             aTAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCa                                                                                                                       >  2:1032488/1‑140 (MQ=255)

             aTAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCa                                                                                                                       >  1:80666/1‑140 (MQ=255)

                  aaTTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGt                                                                                                                  <  2:139457/140‑1 (MQ=255)

                   aTTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTa                                                                                                                 <  2:1738039/140‑1 (MQ=255)

                      tACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTaaaa                                                                                                              >  1:1118799/1‑140 (MQ=255)

                      tACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTaaaa                                                                                                              >  2:333958/1‑140 (MQ=255)

                              tATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGa                                                                                                      <  2:750954/140‑1 (MQ=255)

                               aTTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGaa                                                                                                     >  2:1241246/1‑140 (MQ=255)

                               aTTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGaa                                                                                                     >  2:597708/1‑140 (MQ=255)

                                ttGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAAc                                                                                                    <  1:1399207/140‑1 (MQ=255)

                                 tgatgaGAGTTTGGTGTCTTTATATGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACt                                                                                                   <  1:1833148/140‑1 (MQ=255)

                                    tgaGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTtg                                                                                                <  1:1690117/140‑1 (MQ=255)

                                     gagaGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTa                                                                                                          <  2:151727/129‑1 (MQ=255)

                                     gagaGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTtgt                                                                                               >  2:1941229/1‑140 (MQ=255)

                                       gagTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGa                                                                                             <  1:2440712/140‑1 (MQ=255)

                                        ggTTTGGTTTCTTTATTGGGCTGGCGGGAACTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGaa                                                                                            <  2:1958747/139‑1 (MQ=255)

                                        agTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGaa                                                                                            <  2:2638021/140‑1 (MQ=255)

                                        agTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGaa                                                                                            >  2:2114845/1‑140 (MQ=255)

                                        agTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGaa                                                                                            >  2:2061425/1‑140 (MQ=255)

                                          tttGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGaa                                                                                            <  1:485410/138‑1 (MQ=255)

                                          tttGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGaa                                                                                            <  2:1903364/138‑1 (MQ=255)

                                          tttGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGaa                                                                                            >  1:1903364/1‑138 (MQ=255)

                                          tttGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGaa                                                                                            >  2:485410/1‑138 (MQ=255)

                                          tttGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAaca                                                                                          >  2:549129/1‑140 (MQ=255)

                                          tttGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAaca                                                                                          >  2:1105475/1‑140 (MQ=255)

                                          tttGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAaca                                                                                          >  1:925702/1‑140 (MQ=255)

                                          tttGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAaca                                                                                          >  2:1445963/1‑140 (MQ=255)

                                          tttGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAaca                                                                                          >  2:2704278/1‑140 (MQ=255)

                                          tttGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAaca                                                                                          >  2:2218713/1‑140 (MQ=255)

                                          tttGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAaca                                                                                          >  2:1550521/1‑140 (MQ=255)

                                          tttGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAaca                                                                                          >  1:241798/1‑140 (MQ=255)

                                           ttGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAacac                                                                                         <  1:2704278/140‑1 (MQ=255)

                                           ttGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAacac                                                                                         <  2:2616536/140‑1 (MQ=255)

                                             ggTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGaa                                                                                            >  1:233665/1‑135 (MQ=255)

                                             ggTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGaa                                                                                            <  2:233665/135‑1 (MQ=255)

                                             ggTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCg                                                                                       >  1:1509841/1‑140 (MQ=255)

                                             ggTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCg                                                                                       >  2:491427/1‑140 (MQ=255)

                                              gtgtCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGt                                                                                      <  2:646683/140‑1 (MQ=255)

                                               tgtCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGA‑TTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCa                                                                                    <  1:1937589/140‑1 (MQ=255)

                                                gtCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCa                                                                                    <  1:2218713/140‑1 (MQ=255)

                                                  cTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCata                                                                                  >  1:1727752/1‑140 (MQ=255)

                                                  cTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCata                                                                                  >  1:1702553/1‑140 (MQ=255)

                                                  cTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCata                                                                                  >  1:933623/1‑140 (MQ=255)

                                                   tttATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCatat                                                                                 >  1:2008363/1‑140 (MQ=255)

                                                   tttATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCatat                                                                                 <  2:2448493/140‑1 (MQ=255)

                                                   tttATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCatat                                                                                 <  2:80666/140‑1 (MQ=255)

                                                    ttATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCatat                                                                                 <  2:1088524/139‑1 (MQ=255)

                                                         tgtCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCAt                                                                           >  1:2413945/1‑140 (MQ=255)

                                                           tCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCa                                                                                    <  1:1465652/129‑1 (MQ=255)

                                                             tGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATagag                                                                       <  1:29256/140‑1 (MQ=255)

                                                             tGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATagag                                                                       <  1:2138446/140‑1 (MQ=255)

                                                             tGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATagag                                                                       >  2:81069/1‑140 (MQ=255)

                                                               cAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCatat                                                                                 >  2:1875137/1‑128 (MQ=255)

                                                               cAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCatat                                                                                 <  1:1875137/128‑1 (MQ=255)

                                                               cAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGAc                                                                     >  1:2642844/1‑140 (MQ=255)

                                                                aGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACg                                                                    <  2:2008363/140‑1 (MQ=255)

                                                                   cATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGtga                                                                 >  1:2265424/1‑140 (MQ=255)

                                                                    aTCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGtgat                                                                <  1:1550521/140‑1 (MQ=255)

                                                                     tCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGtgatg                                                               <  1:702164/140‑1 (MQ=255)

                                                                     tCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGtgatg                                                               <  2:2413945/140‑1 (MQ=255)

                                                                     tCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGtgatg                                                               >  1:1737828/1‑140 (MQ=255)

                                                                     tCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGtgatg                                                               >  1:110161/1‑140 (MQ=255)

                                                                       tttCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTgatgat                                                             >  1:2505962/1‑140 (MQ=255)

                                                                          ccATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATAtt                                                          <  2:110161/140‑1 (MQ=255)

                                                                           cATTCAAACTAACGTTTCGCTACCGTGAAAGTGCGACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATAttt                                                         <  2:2418385/140‑1 (MQ=255)

                                                                            aTTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGAAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTa                                                        >  2:2030836/1‑140 (MQ=255)

                                                                             ttCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTAc                                                       >  2:276142/1‑140 (MQ=255)

                                                                                  aCTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGtgat                                                                >  2:93883/1‑126 (MQ=255)

                                                                                       cGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAg                                                     >  1:981034/1‑132 (MQ=255)

                                                                                       cGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTa                                             <  1:594015/140‑1 (MQ=255)

                                                                                        gTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTaa                                            <  2:1512966/140‑1 (MQ=255)

                                                                                         tttCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAAt                                           <  1:101586/140‑1 (MQ=255)

                                                                                         tttCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAAt                                           <  1:1241246/140‑1 (MQ=255)

                                                                                         tttCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAAt                                           <  2:557498/140‑1 (MQ=255)

                                                                                         tttCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAAt                                           <  1:679813/140‑1 (MQ=255)

                                                                                         tttCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAAt                                           <  1:529431/140‑1 (MQ=255)

                                                                                         tttCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAAt                                           <  2:2166803/140‑1 (MQ=255)

                                                                                          ttCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATc                                          <  1:2061425/140‑1 (MQ=255)

                                                                                           tCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATCt                                         <  1:276142/140‑1 (MQ=255)

                                                                                            cGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAg                                                     >  1:325878/1‑127 (MQ=255)

                                                                                            cGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATCTa                                        <  2:1727752/140‑1 (MQ=255)

                                                                                                  cGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATCTATTtata                                  >  2:1545351/1‑140 (MQ=255)

                                                                                                    tGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATCTATTTATATg                                >  1:435695/1‑140 (MQ=255)

                                                                                                     gAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATCTATTTATATGa                               <  2:726790/140‑1 (MQ=255)

                                                                                                     gAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATCTATTTATATGa                               <  2:2265424/140‑1 (MQ=255)

                                                                                                      aaaGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATCTATTTATATGAt                              <  1:265166/140‑1 (MQ=255)

                                                                                                       aaGTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATCTATTTATATGAtt                             <  1:1545351/140‑1 (MQ=255)

                                                                                                         gTGCTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATCTATTTATATGATTTc                           >  1:1050875/1‑140 (MQ=255)

                                                                                                            cTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATCTATTTATATGATTTCCtt                        <  1:549129/140‑1 (MQ=255)

                                                                                                            cTACAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATCTATTTATATGATTTCCt                         >  1:1090181/1‑139 (MQ=255)

                                                                                                               cAAAGATAAAGATTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATCTATTTATATGATTTCCTtata                     <  1:2114845/140‑1 (MQ=255)

                                                                                                                    ataaagatTTGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATCTATTTATATGATTTCCTTATATTTaa                <  2:515803/140‑1 (MQ=255)

                                                                                                                            ttGGTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATCTATTTATATGATTTCCTTATATTTAAATTAACTa        >  1:1411841/1‑140 (MQ=255)

                                                                                                                              ggTTTCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATCTATTTATATGATTTCCTTATATTTAAATTAACTaaa      >  2:320128/1‑140 (MQ=255)

                                                                                                                                  tCAAAAAG‑AAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATCTATTTATATGATTTCCTTATATTTAAATTAACTAAACGGa  <  2:1297785/140‑1 (MQ=255)

ATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATCTATTTATATGATTTCCTTATATTTAAATTAACTAAACGGA  >  NC_000913/987031‑987301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`