Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 232,873 G→T S361R (AGC→AGA)  mltD ← membrane‑bound lytic murein transglycosylase D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913232,8730GT100.0% 548.5 / NA 154S361R (AGC→AGA) mltDmembrane‑bound lytic murein transglycosylase D
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (80/74);  total (80/74)

Only 100 of 159 total aligned reads displayed.

TTTGCGCACACGATACGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGTCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCAC  >  NC_000913/232738‑233009
                                                                                                                                       |                                                                                                                                        
tttGCGCACACGATACGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTa                                                                                                                                      >  2:2299309/1‑140 (MQ=255)
 ttGCGCACACGATACGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTAc                                                                                                                                     >  2:1923242/1‑140 (MQ=255)
  tGCGCACACGATACGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACg                                                                                                                                    <  1:2169289/140‑1 (MQ=255)
   gcgcACACGATACGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGc                                                                                                                                   >  1:1228072/1‑140 (MQ=255)
    cgcACACGATACGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGcc                                                                                                                                  >  2:2494087/1‑140 (MQ=255)
              aCGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGaa                                                                                                                        >  2:2560597/1‑140 (MQ=255)
               cGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAg                                                                                                                       <  1:1720798/140‑1 (MQ=255)
               cGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAg                                                                                                                       <  2:1108801/140‑1 (MQ=255)
                 tAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCg                                                                                                                     <  2:1529719/140‑1 (MQ=255)
                   aTGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGt                                                                                                                           >  1:1109743/1‑132 (MQ=255)
                   aTGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCg                                                                                                                     <  2:765218/138‑1 (MQ=255)
                   aTGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGAt                                                                                                                   <  1:1328185/140‑1 (MQ=255)
                   aTGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGAt                                                                                                                   >  2:558608/1‑140 (MQ=255)
                   aTGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGAt                                                                                                                   >  1:946223/1‑140 (MQ=255)
                   aTGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGAt                                                                                                                   <  1:2299309/140‑1 (MQ=255)
                     gCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATAc                                                                                                                 >  1:285607/1‑140 (MQ=255)
                     gCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATAc                                                                                                                 >  2:2741133/1‑140 (MQ=255)
                     gCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATAc                                                                                                                 >  2:1448547/1‑140 (MQ=255)
                     gCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATAc                                                                                                                 >  2:927285/1‑140 (MQ=255)
                     gCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGCTCTTACGCCGAGACGTGAAGCGATAc                                                                                                                 >  1:2528553/1‑140 (MQ=255)
                       tATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGTCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACtt                                                                                                               >  2:540238/1‑140 (MQ=255)
                       tATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCg                                                                                                                     <  1:1544246/134‑1 (MQ=255)
                       tATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCg                                                                                                                     >  2:1544246/1‑134 (MQ=255)
                       tATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACtt                                                                                                               >  1:1838184/1‑140 (MQ=255)
                       tATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACtt                                                                                                               >  2:1657122/1‑140 (MQ=255)
                        aTCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTg                                                                                                              >  2:1642535/1‑140 (MQ=255)
                         tCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGa                                                                                                             >  2:154052/1‑140 (MQ=255)
                         tCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGa                                                                                                             >  2:704983/1‑140 (MQ=255)
                         tCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGa                                                                                                             >  1:1061645/1‑140 (MQ=255)
                         tCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTATGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGa                                                                                                             >  1:1412299/1‑140 (MQ=255)
                          cGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGaa                                                                                                            >  1:22383/1‑140 (MQ=255)
                          cGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGaa                                                                                                            <  2:118616/140‑1 (MQ=255)
                          cGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGaa                                                                                                            >  2:1142984/1‑140 (MQ=255)
                          cGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGaa                                                                                                            <  1:2494087/140‑1 (MQ=255)
                          cGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGaa                                                                                                            <  1:154052/140‑1 (MQ=255)
                           gCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGaaa                                                                                                           >  2:2224779/1‑140 (MQ=255)
                                 gttTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCgtgt                                                                                                     >  2:132074/1‑140 (MQ=255)
                                    tGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCTCTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGc                                                                                                  >  1:792704/1‑140 (MQ=255)
                                    tGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGc                                                                                                  >  1:2352203/1‑140 (MQ=255)
                                     gCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGcc                                                                                                 <  1:132074/140‑1 (MQ=255)
                                         gTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCagag                                                                                             >  2:2029280/1‑140 (MQ=255)
                                          tCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGc                                                                                            >  2:2750324/1‑140 (MQ=255)
                                          tCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGc                                                                                            >  2:359476/1‑140 (MQ=255)
                                          tCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGc                                                                                            >  2:1099958/1‑140 (MQ=255)
                                            gCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGt                                                                                          <  2:1678057/140‑1 (MQ=255)
                                            gCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGt                                                                                          <  1:927285/140‑1 (MQ=255)
                                             cTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTa                                                                                         <  2:22383/140‑1 (MQ=255)
                                             cTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTa                                                                                         <  2:1061645/140‑1 (MQ=255)
                                             cTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTa                                                                                         <  2:531568/140‑1 (MQ=255)
                                             cTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTa                                                                                         <  1:2750324/140‑1 (MQ=255)
                                              tgtgCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTAc                                                                                        <  2:2449178/140‑1 (MQ=255)
                                              tgtgCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTAc                                                                                        <  1:662647/140‑1 (MQ=255)
                                                   gcTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGgtgt                                                                                   <  1:359476/140‑1 (MQ=255)
                                                    cTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTATGGTGTa                                                                                  >  2:2043037/1‑140 (MQ=255)
                                                    cTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTa                                                                                  >  1:429871/1‑140 (MQ=255)
                                                     tACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTa                                                                                         >  2:249848/1‑132 (MQ=255)
                                                          cctgcACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTATGGTGTAAACACg                                                                            >  1:2068265/1‑140 (MQ=255)
                                                          cctgcACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACg                                                                            >  2:279552/1‑140 (MQ=255)
                                                          cctgcACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACg                                                                            >  2:1963609/1‑140 (MQ=255)
                                                           ctgcACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACgg                                                                           <  1:1253987/140‑1 (MQ=255)
                                                               aCCAATCGTCAAACTTTAGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGt                                                                       >  1:822414/1‑140 (MQ=255)
                                                                ccAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCCCTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGtt                                                                      <  1:540238/140‑1 (MQ=255)
                                                                  aaTCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTaa                                                                    <  1:1963609/140‑1 (MQ=255)
                                                                   aTCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAg                                                                   >  2:1444130/1‑140 (MQ=255)
                                                                   aTCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAg                                                                   <  2:2352203/140‑1 (MQ=255)
                                                                   aTCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAg                                                                   <  2:946223/140‑1 (MQ=255)
                                                                    tCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAgc                                                                  >  1:1607179/1‑140 (MQ=255)
                                                                      gTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTATGGTGTAAACACGGCTGTTAAgcgg                                                                <  2:2068265/140‑1 (MQ=255)
                                                                       tCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAgcggc                                                               <  1:1947462/140‑1 (MQ=255)
                                                                       tCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGAGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAgcggc                                                               <  1:2646880/140‑1 (MQ=255)
                                                                         aaaCTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGt                                                             >  2:1686815/1‑140 (MQ=255)
                                                                           aCTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTAt                                                           <  1:747132/140‑1 (MQ=255)
                                                                           aCTATGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTAt                                                           <  2:1412299/140‑1 (MQ=255)
                                                                             tttGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTg                                                         <  2:2665806/140‑1 (MQ=255)
                                                                             tttGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTg                                                         <  2:742799/140‑1 (MQ=255)
                                                                              ttGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGt                                                        <  1:1000669/140‑1 (MQ=255)
                                                                                    tGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGTCGGCaa                                                  <  2:1838184/140‑1 (MQ=255)
                                                                                    tGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGTCGGCaa                                                  <  2:285607/140‑1 (MQ=255)
                                                                                         tCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGTCGGCAACCAGc                                             <  2:839139/140‑1 (MQ=255)
                                                                                                 gATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGTCGGCAACCAGCGTCGACTg                                     <  1:1642535/140‑1 (MQ=255)
                                                                                                 gATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGTCGGCAACCAGCGTCGACTg                                     >  1:427697/1‑140 (MQ=255)
                                                                                                     cgcgCAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGTCGGCAACCAGCGTCGACTGTAca                                 >  1:696922/1‑140 (MQ=255)
                                                                                                       cgcAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGTCGGCAACCAGCGTCGACTGTAcagc                               >  1:2477583/1‑140 (MQ=255)
                                                                                                       cgcAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGTCGGCAACCAGCGTCGACTGTAcagc                               >  2:781376/1‑140 (MQ=255)
                                                                                                       cgcAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGTCGGCAACCAGCGTCGACTGTAcagc                               >  2:1129340/1‑140 (MQ=255)
                                                                                                         cAGTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGTCGGCAACCAGCGTCGACTGTAcagcag                             <  1:704983/140‑1 (MQ=255)
                                                                                                           gTTTGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGTCGGCAACCAGCGTCGACTGTACAgcagca                           >  1:1233537/1‑140 (MQ=255)
                                                                                                            tttGTTCCACTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGTCGGCAACCAGCGTCGACTGTACAGCAGCaa                          >  1:2722601/1‑140 (MQ=255)
                                                                                                                   cACTGCTGCAAATCTTTGGTTCTTACACCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGTCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGcc                   <  1:2400160/140‑1 (MQ=255)
                                                                                                                    aCTGCTGCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTATGGTGTAAACACGGCTGTTAAGCGGCGTATTGTCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCt                  <  1:2043037/140‑1 (MQ=255)
                                                                                                                     ctgctgCAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGTCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTg                 <  1:1448547/140‑1 (MQ=255)
                                                                                                                      tgctgcAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGTCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGa                >  1:278505/1‑140 (MQ=255)
                                                                                                                          gcAAATCTTTGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGTCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGcc            >  1:1401155/1‑140 (MQ=255)
                                                                                                                           gaaaTCTTTGGTTCTTACGCCGAGACGTGAAGCGATGCTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGTCGGCAACCAGCGTCGTCTGTACAGCAGCAATTTCGCCTGAAGCCa           <  2:931173/139‑1 (MQ=255)
                                                                                                                                  ttGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGTCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCa           >  1:1660511/1‑133 (MQ=255)
                                                                                                                                  ttGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGTCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTc    >  1:261403/1‑140 (MQ=255)
                                                                                                                                   tGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGTCGGCAACCAGCGTCGACTGTACAGCCGCAATTTCGCCTGAAGCCAGAGATTCa   <  2:375119/140‑1 (MQ=255)
                                                                                                                                   tGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGTCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCa   <  2:2631955/140‑1 (MQ=255)
                                                                                                                                   tGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGTCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCa   <  1:1129340/140‑1 (MQ=255)
                                                                                                                                   tGGTTCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGTCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCa   <  1:2271908/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                        
TTTGCGCACACGATACGTAATGCTATCGCTGTTGTTTGCCAGTCGCTGTGCGCTACTGCCTGCACCAATCGTCAAACTTTGGCCTGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGTCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCAC  >  NC_000913/232738‑233009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑