Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,457,501 G→T A553A (GCC→GCA)  fadJ ← 3‑hydroxyacyl‑CoA dehydrogenase FadJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,457,5010GT100.0% 400.0 / NA 114A553A (GCC→GCA) fadJ3‑hydroxyacyl‑CoA dehydrogenase FadJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (53/61);  total (53/61)

Only 100 of 117 total aligned reads displayed.

GGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCGGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGTTCACCTTGGGTCAACA  >  NC_000913/2457364‑2457634
                                                                                                                                         |                                                                                                                                     
ggATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGc                                                                                                                                     >  2:1626507/1‑140 (MQ=255)
ggATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGc                                                                                                                                     >  2:592085/1‑140 (MQ=255)
ggATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGc                                                                                                                                     >  1:1576459/1‑140 (MQ=255)
ggATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGc                                                                                                                                     >  2:2465824/1‑140 (MQ=255)
 gATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAgctgct                                                                                                                                    <  2:865244/140‑1 (MQ=255)
  aTCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCtt                                                                                                                                   >  1:2424601/1‑140 (MQ=255)
   tCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTc                                                                                                                                  <  1:2200811/140‑1 (MQ=255)
   tCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTc                                                                                                                                  <  2:749683/140‑1 (MQ=255)
   tCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTc                                                                                                                                  <  2:352349/140‑1 (MQ=255)
   tCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTc                                                                                                                                  <  2:2316882/140‑1 (MQ=255)
     gACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCa                                                                                                                                <  1:1626507/140‑1 (MQ=255)
     gACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCa                                                                                                                                <  1:2465824/140‑1 (MQ=255)
        cTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTa                                                                                                                             <  1:696814/140‑1 (MQ=255)
         tGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTAc                                                                                                                            <  1:371126/140‑1 (MQ=255)
         tGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTAc                                                                                                                            <  2:137607/140‑1 (MQ=255)
         tGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTAc                                                                                                                            <  1:166261/140‑1 (MQ=255)
          gTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACa                                                                                                                           <  1:1386318/140‑1 (MQ=255)
          gTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACa                                                                                                                           <  2:127381/140‑1 (MQ=255)
            tttttGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAgg                                                                                                                         <  2:897549/140‑1 (MQ=255)
               ttGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGaat                                                                                                                      <  1:592085/140‑1 (MQ=255)
               ttGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGaat                                                                                                                      <  1:1244793/140‑1 (MQ=255)
                 gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGaataa                                                                                                                    >  1:1088909/1‑140 (MQ=255)
                            cTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCg                                                                                                         <  1:2534828/140‑1 (MQ=255)
                             tttCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCgg                                                                                                        <  1:35861/140‑1 (MQ=255)
                             tttCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCgg                                                                                                        <  2:929453/140‑1 (MQ=255)
                                cTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCTGTACAGGAATAATTTTAGTCCCGgtgt                                                                                                     <  2:2392553/140‑1 (MQ=255)
                                cTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGgtgt                                                                                                     <  2:216044/140‑1 (MQ=255)
                                  gACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCg                                                                                                   <  1:1958901/140‑1 (MQ=255)
                                       tAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGgtgt                                                                                                     <  1:782292/133‑1 (MQ=255)
                                       tAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGgtgt                                                                                                     >  2:782292/1‑133 (MQ=255)
                                       tAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTcc                                                                                              >  2:1335527/1‑140 (MQ=255)
                                       tAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTcc                                                                                              >  2:2681645/1‑140 (MQ=255)
                                       tAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTcc                                                                                              >  2:1092472/1‑140 (MQ=255)
                                             tAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTc                                                                                        >  2:334866/1‑140 (MQ=255)
                                               gAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCAt                                                                                      >  1:1129013/1‑140 (MQ=255)
                                               gAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCAt                                                                                      >  1:868331/1‑140 (MQ=255)
                                               gAAACCCCGGCCATTTTTTCTGACTTTGCGNTCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGATAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCAt                                                                                      >  2:1969353/1‑140 (MQ=255)
                                                aaaCCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATc                                                                                     >  1:1787279/1‑140 (MQ=255)
                                                 aaCCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATcc                                                                                    <  2:1787279/140‑1 (MQ=255)
                                                  aCCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCa                                                                                   >  2:1397552/1‑140 (MQ=255)
                                                    cccGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCaaa                                                                                 >  2:407421/1‑140 (MQ=255)
                                                     ccGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCaaaa                                                                                >  2:2154168/1‑140 (MQ=255)
                                                      cGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTTCAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAg                                                                               >  2:1513866/1‑140 (MQ=255)
                                                      cGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAg                                                                               >  2:2122137/1‑140 (MQ=255)
                                                      cGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAg                                                                               <  2:868331/140‑1 (MQ=255)
                                                       ggCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGt                                                                              >  1:2655690/1‑140 (MQ=255)
                                                       ggCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGt                                                                              <  1:407421/140‑1 (MQ=255)
                                                        gCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGtt                                                                             >  2:244990/1‑140 (MQ=255)
                                                          cATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTgg                                                                           >  1:1209448/1‑140 (MQ=255)
                                                           aTTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGa                                                                          <  2:1026462/140‑1 (MQ=255)
                                                            ttttttCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGAt                                                                         <  2:2424601/140‑1 (MQ=255)
                                                                   tGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCt                                                                  <  2:152434/140‑1 (MQ=255)
                                                                       tttGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCg                                                              >  2:187009/1‑140 (MQ=255)
                                                                        ttGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCgg                                                             >  1:1769346/1‑140 (MQ=255)
                                                                         tGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGa                                                            >  1:1240843/1‑140 (MQ=255)
                                                                         tGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGa                                                            >  1:402482/1‑140 (MQ=255)
                                                                             aTCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAAc                                                        <  1:686001/140‑1 (MQ=255)
                                                                               cgtcgtTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCa                                                      <  2:1209448/140‑1 (MQ=255)
                                                                               cgtcgtTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCa                                                      <  1:244990/140‑1 (MQ=255)
                                                                                  cgtTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCgg                                                             >  1:1974966/1‑130 (MQ=255)
                                                                                  cgtTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCgg                                                             <  2:1974966/130‑1 (MQ=255)
                                                                                  cgtTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCgg                                                             >  1:1131589/1‑130 (MQ=255)
                                                                                  cgtTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCgg                                                             <  2:1131589/130‑1 (MQ=255)
                                                                                  cgtTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAAt                                                   >  2:2385739/1‑140 (MQ=255)
                                                                                   gtTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAAtt                                                  >  2:2132161/1‑140 (MQ=255)
                                                                                    ttCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAAttt                                                 >  1:2074226/1‑140 (MQ=255)
                                                                                        aaaTTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACt                                             <  1:1769404/140‑1 (MQ=255)
                                                                                          aTTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATGCAAAAGTTGGATTGGGCCCACCGGAAAACCAAATTTCACTAg                                           <  2:1088909/140‑1 (MQ=255)
                                                                                           ttGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAgc                                          >  2:814397/1‑140 (MQ=255)
                                                                                            tGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAgcg                                         <  1:187009/140‑1 (MQ=255)
                                                                                            tGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAgcg                                         <  1:2132161/140‑1 (MQ=255)
                                                                                              aagaaACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAgcg                                         <  2:1657235/138‑1 (MQ=255)
                                                                                              aagaaACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAgcg                                         >  1:1657235/1‑138 (MQ=255)
                                                                                                 aaaCAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCa                                    <  2:2074226/140‑1 (MQ=255)
                                                                                                 aaaCAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCa                                    <  1:1335527/140‑1 (MQ=255)
                                                                                                 aaaCAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCa                                    <  1:842469/140‑1 (MQ=255)
                                                                                                 aaaCAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCa                                    <  1:2154168/140‑1 (MQ=255)
                                                                                                  aacaacATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCAt                                   <  2:2693739/140‑1 (MQ=255)
                                                                                                     aacaTTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCaa                                <  2:1941565/140‑1 (MQ=255)
                                                                                                     aacaTTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCaa                                <  1:1572748/140‑1 (MQ=255)
                                                                                                     aacaTTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCaa                                <  2:1939062/140‑1 (MQ=255)
                                                                                                     aacaTTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCaa                                >  1:1890021/1‑140 (MQ=255)
                                                                                                      acaTTTGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAAt                               <  1:1440346/140‑1 (MQ=255)
                                                                                                           tGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGct                          >  2:755522/1‑140 (MQ=255)
                                                                                                           tGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGct                          >  2:188024/1‑140 (MQ=255)
                                                                                                           tGCAGGCGCGCTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGct                          >  1:300617/1‑140 (MQ=255)
                                                                                                                 cgcgcTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCAt                                   >  1:1691478/1‑125 (MQ=255)
                                                                                                                 cgcgcTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCAt                                   <  2:1691478/125‑1 (MQ=255)
                                                                                                                 cgcgcTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTAccc                    >  2:164483/1‑140 (MQ=255)
                                                                                                                     cTAAAACGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGTTc                >  1:2735520/1‑140 (MQ=255)
                                                                                                                          aCGTTCTCCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGTTCACCtt           <  1:392086/140‑1 (MQ=255)
                                                                                                                              tctcCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGTTCACCTTGGGt       >  2:231882/1‑140 (MQ=255)
                                                                                                                              tctcCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGTTCACCTTGGGt       >  2:2025948/1‑140 (MQ=255)
                                                                                                                              tctcCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGTTCACCTTGGGt       >  1:2671556/1‑140 (MQ=255)
                                                                                                                              tctcCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGTTCACCTTGGGt       >  1:313374/1‑140 (MQ=255)
                                                                                                                               ctcCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGTTCACCTTGGGTc      <  2:878511/140‑1 (MQ=255)
                                                                                                                                tcCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGTTCACCTTGGGTCa     >  2:182096/1‑140 (MQ=255)
                                                                                                                                tcCATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGTTCACCTTGGGTCa     >  1:189365/1‑140 (MQ=255)
                                                                                                                                  cATAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGTTCACCTTGGGTCAAc   >  1:384644/1‑140 (MQ=255)
                                                                                                                                   aTAAGCTGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGTTCACCTTGGGTCAACa  <  2:300617/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                     
GGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCGGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGTTCACCTTGGGTCAACA  >  NC_000913/2457364‑2457634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑