BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,200,061	T→C	G186G (GGT→GGC)	yjaG →	conserved protein YjaG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,200,061	0	T	C	99.3%	547.1 / ‑5.2	148	G186G (GGT→GGC)	yjaG	conserved protein YjaG
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (78/69); total (79/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 150 total aligned reads displayed.

ATGAAGAGCTTAAAGAAAACCCAGCTGTAGAGCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGG  >  NC_000913/4199925‑4200196

aTGAAGAGCTTAAAGAAAACCCAGCTGTAGAGCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCgag                                                                                                                                      <  1:151673/140‑1 (MQ=255)

  gAAGAGCTTAAAGAAAACCCAGCTGTAGAGCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGagag                                                                                                                                    <  1:2569229/140‑1 (MQ=255)

  gAAGAGCTTAAAGAAAACCCAGCTGTAGAGCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGagag                                                                                                                                    <  1:378621/140‑1 (MQ=255)

  gAAGAGCTTAAAGAAAACCCAGCTGTAGAGCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGagag                                                                                                                                    <  1:136761/140‑1 (MQ=255)

   aaGAGCTTAAAGAAAACCCAGCTGTAGAGCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGc                                                                                                                                   <  2:1810597/140‑1 (MQ=255)

   aaGAGCTTAAAGAAAACCCAGCTGTAGAGCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGc                                                                                                                                   <  1:1653077/140‑1 (MQ=255)

   aaGAGCTTAAAGAAAACCCAGCTGTAGAGCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGc                                                                                                                                   <  2:2639077/140‑1 (MQ=255)

      agCTTAAAGAAAACCCAGCTGTAGAGCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAat                                                                                                                                <  2:1037025/140‑1 (MQ=255)

        cTTAAAGAAAACCCAGCTGTAGAGCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAatat                                                                                                                              <  2:2406838/140‑1 (MQ=255)

              gAAAACCCAGCTGTAGAGCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGtat                                                                                                                        <  1:2308835/140‑1 (MQ=255)

                      aGCTGTAGAGCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTc                                                                                                                <  1:2580583/140‑1 (MQ=255)

                      aGCTGTAGAGCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTc                                                                                                                <  1:2542046/140‑1 (MQ=255)

                        cTGTAGAGCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAg                                                                                                              >  1:1159270/1‑140 (MQ=255)

                         tGTAGAGCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCa                                                                                                               <  1:2727741/138‑1 (MQ=255)

                         tGTAGAGCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCa                                                                                                               >  2:2727741/1‑138 (MQ=255)

                         tGTAGAGCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGc                                                                                                             >  2:2031048/1‑140 (MQ=255)

                           tAGAGCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCaa                                                                                                           <  1:2328937/140‑1 (MQ=255)

                            agagCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATGAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAAt                                                                                                          <  2:2065434/140‑1 (MQ=255)

                                  aGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGAcc                                                                                                    >  1:497456/1‑140 (MQ=255)

                                   gAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCa                                                                                                   <  1:2076298/140‑1 (MQ=255)

                                     aTGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGa                                                                                                 >  1:182964/1‑140 (MQ=255)

                                      tGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGaa                                                                                                <  2:2229724/140‑1 (MQ=255)

                                           cATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGt                                                                                           <  2:463852/140‑1 (MQ=255)

                                              tCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGAt                                                                                        >  2:1583408/1‑140 (MQ=255)

                                                 gTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTa                                                                                     >  2:530417/1‑140 (MQ=255)

                                                  tGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTaa                                                                                    >  1:2084409/1‑140 (MQ=255)

                                                  tGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTaa                                                                                    >  1:1893590/1‑140 (MQ=255)

                                                  tGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTaa                                                                                    >  2:118136/1‑140 (MQ=255)

                                                  tGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTaa                                                                                    >  1:1505750/1‑140 (MQ=255)

                                                   gggAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAAc                                                                                   >  1:963877/1‑140 (MQ=255)

                                                     gAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCATTATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGc                                                                                 <  1:1071922/140‑1 (MQ=255)

                                                     gAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGc                                                                                 <  1:19117/140‑1 (MQ=255)

                                                     gAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGc                                                                                 <  2:105835/140‑1 (MQ=255)

                                                       aaTATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCt                                                                               >  1:895552/1‑140 (MQ=255)

                                                         tatTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGa                                                                             <  1:1173948/140‑1 (MQ=255)

                                                          atTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGAt                                                                            <  2:797188/140‑1 (MQ=255)

                                                            tCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGAttt                                                                          <  2:490903/140‑1 (MQ=255)

                                                            tCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGAt                                                                            >  2:1877093/1‑138 (MQ=255)

                                                             ccGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTg                                                                         <  1:1132217/140‑1 (MQ=255)

                                                              cGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGAt                                                                            <  2:51224/136‑1 (MQ=255)

                                                              cGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGAt                                                                            >  1:51224/1‑136 (MQ=255)

                                                              cGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGt                                                                        <  2:1893590/140‑1 (MQ=255)

                                                              cGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGt                                                                        >  1:1033815/1‑140 (MQ=255)

                                                              cGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGt                                                                        >  1:1752269/1‑140 (MQ=255)

                                                                aCTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGt                                                                        >  1:1332290/1‑138 (MQ=255)

                                                                aCTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGt                                                                        <  2:1332290/138‑1 (MQ=255)

                                                                aCTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCg                                                                      >  1:1007917/1‑140 (MQ=255)

                                                                     tAGCTGAGTGTGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTAcc                                                                  >  1:2381505/1‑139 (MQ=255)

                                                                     tAGCTGAGTGTGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTAcc                                                                  <  2:2381505/139‑1 (MQ=255)

                                                                     tAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGTGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTTTTATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCt                                                                 >  1:2768545/1‑140 (MQ=255)

                                                                      aGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTg                                                                >  2:2003007/1‑140 (MQ=255)

                                                                           aGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTc                                                           >  2:702483/1‑140 (MQ=255)

                                                                           aGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTc                                                           >  2:2542697/1‑140 (MQ=255)

                                                                            gTGCGAAGAACGCGATATCGAGCTGATAAAAGTCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCt                                                          >  2:2062295/1‑140 (MQ=255)

                                                                             tGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCtt                                                         >  2:1676864/1‑140 (MQ=255)

                                                                                gaagaaCGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTccc                                                      <  1:1583408/140‑1 (MQ=255)

                                                                                   gaaCGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTGCCttt                                                   <  2:1378951/140‑1 (MQ=255)

                                                                                      cgcgATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGc                                                <  2:1921876/140‑1 (MQ=255)

                                                                                      cgcgATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGc                                                >  2:148921/1‑140 (MQ=255)

                                                                                      cgcgATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGc                                                <  2:963877/140‑1 (MQ=255)

                                                                                             tCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGt                                         <  1:148921/140‑1 (MQ=255)

                                                                                             tCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGt                                         <  1:45699/140‑1 (MQ=255)

                                                                                              cGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGtt                                        >  2:564495/1‑139 (MQ=255)

                                                                                              cGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTc                                        <  1:1985694/140‑1 (MQ=255)

                                                                                              cGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTc                                        <  2:810001/140‑1 (MQ=255)

                                                                                              cGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTc                                        >  1:810001/1‑140 (MQ=255)

                                                                                              cGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTc                                        >  2:111246/1‑140 (MQ=255)

                                                                                                aGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTg                                      >  2:408377/1‑140 (MQ=255)

                                                                                                  cTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGt                                    <  2:1775478/140‑1 (MQ=255)

                                                                                                  cTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGt                                    <  2:1033815/140‑1 (MQ=255)

                                                                                                     aTAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTc                                        >  1:2406265/1‑133 (MQ=255)

                                                                                                     aTAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTg                                      <  2:884756/135‑1 (MQ=255)

                                                                                                     aTAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTg                                      >  1:884756/1‑135 (MQ=255)

                                                                                                          aGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACGttt                            >  2:2718572/1‑140 (MQ=255)

                                                                                                            gCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTgg                          >  1:413871/1‑140 (MQ=255)

                                                                                                               ttAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGt                                         <  1:1360433/122‑1 (MQ=255)

                                                                                                               ttAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGt                                         >  2:1360433/1‑122 (MQ=255)

                                                                                                               ttAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTggggg                       >  2:2161171/1‑140 (MQ=255)

                                                                                                                    gCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGaa                  <  1:1967162/140‑1 (MQ=255)

                                                                                                                      aGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGaaaa                >  2:41596/1‑140 (MQ=255)

                                                                                                                       gACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGaaaaa               >  1:638686/1‑140 (MQ=255)

                                                                                                                       gACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGaaaaa               >  2:1644409/1‑140 (MQ=255)

                                                                                                                       gACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGaaaaa               >  1:2203349/1‑140 (MQ=255)

                                                                                                                        aCCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGaaaaaa              >  2:2362422/1‑140 (MQ=255)

                                                                                                                            gCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTg          >  1:1633846/1‑140 (MQ=255)

                                                                                                                             cGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTgg         <  1:530417/140‑1 (MQ=255)

                                                                                                                             cGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTgg         >  2:982495/1‑140 (MQ=255)

                                                                                                                             cGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTgg         >  1:2214376/1‑140 (MQ=255)

                                                                                                                             cGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTgg         >  2:1196831/1‑140 (MQ=255)

                                                                                                                             cGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTgg         >  2:2568970/1‑140 (MQ=255)

                                                                                                                             cGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTgg         >  2:2445755/1‑140 (MQ=255)

                                                                                                                             cGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTgg         >  2:1568652/1‑140 (MQ=255)

                                                                                                                             cGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTgg         >  1:2522421/1‑140 (MQ=255)

                                                                                                                             cGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTgg         >  2:2175946/1‑140 (MQ=255)

                                                                                                                             cGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTgg         >  2:2095959/1‑140 (MQ=255)

                                                                                                                               tGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCt       >  2:1940644/1‑140 (MQ=255)

                                                                                                                                 aGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTAt     <  2:884788/140‑1 (MQ=255)

                                                                                                                                  ggcgggcgAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATc    >  1:664797/1‑140 (MQ=255)

                                                                                                                                   gCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCg   >  2:2599974/1‑140 (MQ=255)

                                                                                                                                    cGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCgg  <  1:1314854/140‑1 (MQ=255)

ATGAAGAGCTTAAAGAAAACCCAGCTGTAGAGCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGG  >  NC_000913/4199925‑4200196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`