BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,424,472	G→T	intergenic (+86/+44)	ulaF → / ← yjfY	L‑ribulose‑5‑phosphate 4‑epimerase UlaF/DUF1471 domain‑containing protein YjfY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,424,472	0	G	T	100.0%	609.6 / NA	176	intergenic (+86/+44)	ulaF/yjfY	L‑ribulose‑5‑phosphate 4‑epimerase UlaF/DUF1471 domain‑containing protein YjfY
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (84/92); total (84/92)

Only 100 of 182 total aligned reads displayed.

TAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCA  >  NC_000913/4424335‑4424606

tAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAggtgg                                                                                                                                      <  2:331676/140‑1 (MQ=255)

tAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAggtgg                                                                                                                                      >  2:2274807/1‑140 (MQ=255)

tAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAggtgg                                                                                                                                      >  1:1155474/1‑140 (MQ=255)

     acTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGgcaaag                                                                                                                                 <  1:1650149/140‑1 (MQ=255)

     acTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGgcaaag                                                                                                                                 <  2:2394574/140‑1 (MQ=255)

     acTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGgcaaag                                                                                                                                 >  1:1943699/1‑140 (MQ=255)

     acTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGgcaaag                                                                                                                                 <  2:1943699/140‑1 (MQ=255)

     acTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGgcaaag                                                                                                                                 >  1:2152650/1‑140 (MQ=255)

       ttCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGgcaaagca                                                                                                                               >  2:1886974/1‑140 (MQ=255)

        tCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGgcaaagcaa                                                                                                                              >  2:448091/1‑140 (MQ=255)

        tCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGgcaaagcaa                                                                                                                              >  2:1144820/1‑140 (MQ=255)

        tCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGgcaaagca                                                                                                                               >  2:1405817/1‑139 (MQ=255)

         cATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGgcaaagcaaa                                                                                                                             >  2:1624704/1‑140 (MQ=255)

         cATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGgcaaagcaaa                                                                                                                             >  2:2442003/1‑140 (MQ=255)

          aTGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAAc                                                                                                                            >  2:929575/1‑140 (MQ=255)

          aTGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAAc                                                                                                                            >  2:314903/1‑140 (MQ=255)

          aTGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAAc                                                                                                                            >  1:1838755/1‑140 (MQ=255)

          aTGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAG‑CCCCTGCGAAGGTGGCAAAGCAAACt                                                                                                                           >  2:1100465/1‑140 (MQ=255)

           tGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACt                                                                                                                           >  1:490754/1‑140 (MQ=255)

               tAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATgg                                                                                                                       <  1:1624704/140‑1 (MQ=255)

                 aaCACGGTGCTAATGTGTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCTGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCa                                                                                                                     <  1:1669714/140‑1 (MQ=255)

                 aaCACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCa                                                                                                                     >  1:1415199/1‑140 (MQ=255)

                      ggTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGtt                                                                                                                >  2:1558540/1‑140 (MQ=255)

                      ggTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGtt                                                                                                                >  1:2408599/1‑140 (MQ=255)

                        tCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTc                                                                                                              >  2:1468464/1‑140 (MQ=255)

                        tCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTc                                                                                                              >  2:1839471/1‑140 (MQ=255)

                        tCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTc                                                                                                              >  2:2237233/1‑140 (MQ=255)

                        tCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTc                                                                                                              >  2:1268831/1‑140 (MQ=255)

                        tCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAG‑CCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCg                                                                                                             <  1:1100465/140‑1 (MQ=255)

                         ccTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCg                                                                                                             <  1:1268831/140‑1 (MQ=255)

                         ccTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCg                                                                                                             <  2:1234199/140‑1 (MQ=255)

                         ccTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCg                                                                                                             <  1:929575/140‑1 (MQ=255)

                         ccTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCg                                                                                                             <  2:1155474/140‑1 (MQ=255)

                         ccTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCg                                                                                                             <  1:2567799/140‑1 (MQ=255)

                         ccTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCg                                                                                                             <  1:853744/140‑1 (MQ=255)

                          cTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTc                                                                                                              >  1:1027775/1‑138 (MQ=255)

                          cTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTc                                                                                                              <  2:1027775/138‑1 (MQ=255)

                          cTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGt                                                                                                            >  2:814062/1‑140 (MQ=255)

                            aaCGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTg                                                                                                          >  2:38615/1‑140 (MQ=255)

                               gCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGtt                                                                                                       <  1:2274807/140‑1 (MQ=255)

                                  tattaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCa                                                                                                                     >  1:149907/1‑123 (MQ=255)

                                  tattaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCg                                                                                                             <  2:652784/131‑1 (MQ=255)

                                  tattaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCg                                                                                                             >  1:652784/1‑131 (MQ=255)

                                  tattaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGt                                                                                                            >  2:2730965/1‑132 (MQ=255)

                                  tattaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGt                                                                                                            >  1:431957/1‑132 (MQ=255)

                                  tattaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGt                                                                                                            <  1:2730965/132‑1 (MQ=255)

                                  tattaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGt                                                                                                            >  2:1569376/1‑132 (MQ=255)

                                  tattaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTtata                                                                                                    >  2:1338493/1‑140 (MQ=255)

                                  tattaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTtata                                                                                                    >  1:104400/1‑140 (MQ=255)

                                  tattaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTtata                                                                                                    >  2:1803746/1‑140 (MQ=255)

                                  tattaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTtata                                                                                                    >  1:1001041/1‑140 (MQ=255)

                                  tattaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTtata                                                                                                    >  2:859886/1‑140 (MQ=255)

                                  tattaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTtata                                                                                                    >  1:1893448/1‑140 (MQ=255)

                                    ttatgggCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCg                                                                                                             >  1:957963/5‑129 (MQ=255)

                                    ttaTGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCg                                                                                                             <  2:957963/129‑1 (MQ=255)

                                     taCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCt                                                                                                 <  2:2075655/140‑1 (MQ=255)

                                         ggCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTg                                                                                             <  2:2178833/140‑1 (MQ=255)

                                         ggCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTg                                                                                             <  1:1338493/140‑1 (MQ=255)

                                         ggCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTg                                                                                             <  1:214683/140‑1 (MQ=255)

                                         ggCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTg                                                                                             >  1:2529151/1‑140 (MQ=255)

                                           cAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCt                                                                                           <  1:2219109/140‑1 (MQ=255)

                                             gAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAg                                                                                         <  1:2630296/140‑1 (MQ=255)

                                               aGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATCCCTGGTGCTAGCg                                                                                       <  1:1109525/140‑1 (MQ=255)

                                                    aaCACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGc                                                                                            >  1:1407175/1‑130 (MQ=255)

                                                      cacGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAata                                                                                >  1:2283401/1‑140 (MQ=255)

                                                        cgcgCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATc                                                                              >  1:2032083/1‑140 (MQ=255)

                                                        cgcgCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATc                                                                              >  2:1548803/1‑140 (MQ=255)

                                                         gcgcTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCt                                                                                           >  1:98322/1‑126 (MQ=255)

                                                         gcgcTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATcc                                                                             >  1:1241017/1‑140 (MQ=255)

                                                          cgcTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAatat                                                                               <  1:819165/137‑1 (MQ=255)

                                                           gcTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCcg                                                                            >  1:1701680/1‑140 (MQ=255)

                                                           gcTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCcgc                                                                           >  1:877465/1‑140 (MQ=255)

                                                           gcTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCcgc                                                                           >  2:2583546/1‑140 (MQ=255)

                                                           gcTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑‑AAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCcgcg                                                                          >  2:764512/1‑140 (MQ=255)

                                                            cTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCcgcg                                                                          <  1:1144820/140‑1 (MQ=255)

                                                            cTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCcgcg                                                                          <  2:2294348/140‑1 (MQ=255)

                                                            cTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCcgcg                                                                          <  2:2283401/140‑1 (MQ=255)

                                                            cTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCcgcg                                                                          <  1:381467/140‑1 (MQ=255)

                                                            cTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCcgcg                                                                          >  1:785424/1‑140 (MQ=255)

                                                                 gAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGc                                                                     <  1:2237233/140‑1 (MQ=255)

                                                                      ttccttccTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCa                                                                <  2:1241017/140‑1 (MQ=255)

                                                                         cttcctCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCatat                                                             >  2:1398016/1‑140 (MQ=255)

                                                                            cctCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCat                                                               <  2:1678223/135‑1 (MQ=255)

                                                                                       aaCTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTAc                                                    <  1:2083889/135‑1 (MQ=255)

                                                                                            gTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTc                                          >  2:2503784/1‑140 (MQ=255)

                                                                                             tCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTcc                                         <  1:1548803/140‑1 (MQ=255)

                                                                                             tCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTcc                                         <  1:1398016/140‑1 (MQ=255)

                                                                                             tCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTcc                                         <  2:104400/140‑1 (MQ=255)

                                                                                               tAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCg                                       <  1:1882644/140‑1 (MQ=255)

                                                                                               tAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCg                                       >  1:303803/1‑140 (MQ=255)

                                                                                                 aTTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCa                                     >  2:2090019/1‑140 (MQ=255)

                                                                                                      gCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGa                                >  2:2777652/1‑140 (MQ=255)

                                                                                                       cAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGaa                               >  1:1568572/1‑140 (MQ=255)

                                                                                                           cGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTAc                           <  2:423014/140‑1 (MQ=255)

                                                                                                            gTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                          <  1:1909636/140‑1 (MQ=255)

                                                                                                            gTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                          <  2:1506004/140‑1 (MQ=255)

                                                                                                                            gCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTgcg           <  1:764512/140‑1 (MQ=255)

                                                                                                                            gCCCCCTGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTgcg           <  2:877465/140‑1 (MQ=255)

                                                                                                                                  tGCGAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTgc     <  1:2355400/140‑1 (MQ=255)

                                                                                                                                     gAAGGTGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCa  <  2:2152650/140‑1 (MQ=255)

TAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCA  >  NC_000913/4424335‑4424606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`