Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	994,645	G→T	A63E (GCG→GAG)	ssuC ←	aliphatic sulfonate ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	994,645	0	G	T	100.0%	269.8 / NA	80	A63E (GCG→GAG)	ssuC	aliphatic sulfonate ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (41/39);  total (41/39)

GCACGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCGAGGAGGCCAGTTGCCACACCGCCACGATGCCCACCG  >  NC_000913/994511‑994780                                                                                                                                       |                                                                                                                                        gCACGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGa                                                                                                                                    <  2:2549092/140‑1 (MQ=255) gCACGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGa                                                                                                                                    <  1:712132/140‑1 (MQ=255)       tGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGAtgctgc                                                                                                                              >  1:906190/1‑140 (MQ=255)       tGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGAtgctgc                                                                                                                              >  1:151759/1‑140 (MQ=255)       tGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGAtgctgc                                                                                                                              >  1:211300/1‑140 (MQ=255)        gcgcAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGcc                                                                                                                             >  2:506310/1‑140 (MQ=255)         cgcAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCca                                                                                                                            <  1:461166/140‑1 (MQ=255)                 cTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTcgc                                                                                                                    <  2:151759/140‑1 (MQ=255)                   gAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTcgccg                                                                                                                  <  1:135186/140‑1 (MQ=255)                   gAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTcgccg                                                                                                                  >  1:893552/1‑140 (MQ=255)                   gAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTcgccg                                                                                                                  <  1:564217/140‑1 (MQ=255)                   gAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTcgccg                                                                                                                  <  2:381710/140‑1 (MQ=255)                   gAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTcgccg                                                                                                                  <  1:2704346/140‑1 (MQ=255)                      ttGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTg                                                                                                               >  2:927630/1‑140 (MQ=255)                        gAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGc                                                                                                             <  1:2331662/140‑1 (MQ=255)                        gAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGc                                                                                                             >  1:2682939/1‑140 (MQ=255)                        gAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGc                                                                                                             >  2:472792/1‑140 (MQ=255)                        gAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAATTGTTGCCACAGTTCGCCGCTggg                                                                                                             >  1:2494749/1‑139 (MQ=255)                         aGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCg                                                                                                            >  2:90443/1‑140 (MQ=255)                          ggTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCgg                                                                                                           <  1:2414116/140‑1 (MQ=255)                           gTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGga                                                                                                          <  1:137663/140‑1 (MQ=255)                           gTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGga                                                                                                          >  2:1455926/1‑140 (MQ=255)                            tATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGgag                                                                                                         <  2:198523/140‑1 (MQ=255)                              tCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGgag                                                                                                         <  1:1261714/138‑1 (MQ=255)                              tCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGgag                                                                                                         >  2:1261714/1‑138 (MQ=255)                              tCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGagag                                                                                                       >  1:2376053/1‑140 (MQ=255)                              tCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGagag                                                                                                       >  1:868153/1‑140 (MQ=255)                              tCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGagag                                                                                                       >  1:1788372/1‑140 (MQ=255)                                 agcagcCGTTCTCCCCAGCGAGATATCCCGCTAATCAGTCCCAAAATCAACCCCATCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGt                                                                                                    <  1:213479/140‑1 (MQ=255)                                 agcagcCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGt                                                                                                    <  2:2376053/140‑1 (MQ=255)                                   cagcCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGgaga                                                                                                        <  2:685033/134‑1 (MQ=255)                                   cagcCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGgaga                                                                                                        >  1:685033/1‑134 (MQ=255)                                   cagcCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTcc                                                                                                  >  2:2767392/1‑140 (MQ=255)                                   cagcCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTcc                                                                                                  >  2:1409601/1‑140 (MQ=255)                                     gcCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAg                                                                                                <  1:323111/140‑1 (MQ=255)                                      ccGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGa                                                                                               <  1:1409601/140‑1 (MQ=255)                                       cGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGaa                                                                                              <  1:90443/140‑1 (MQ=255)                                        gTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAAc                                                                                             >  1:604362/1‑140 (MQ=255)                                          tctcCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGc                                                                                           <  1:1984480/140‑1 (MQ=255)                                              cccAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTc                                                                                       <  1:2163680/140‑1 (MQ=255)                                              cccAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTc                                                                                       <  2:906190/140‑1 (MQ=255)                                                cAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTcac                                                                                     <  2:694602/140‑1 (MQ=255)                                                 aGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTcacc                                                                                    <  1:902044/140‑1 (MQ=255)                                                  gCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTcacca                                                                                   <  1:599586/140‑1 (MQ=255)                                                   cGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTcaccac                                                                                  <  2:288189/140‑1 (MQ=255)                                                     agaTAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTc                                                                                >  2:1804223/1‑140 (MQ=255)                                                        tAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCtt                                                                             >  2:2086012/1‑140 (MQ=255)                                                        tAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCtt                                                                             >  1:2201384/1‑140 (MQ=255)                                                        tAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCtt                                                                             <  2:1793004/140‑1 (MQ=255)                                                        tAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGACCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCTTCCAGAACGCCGTCACCACTCCtt                                                                             <  2:2682939/140‑1 (MQ=255)                                                         aGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTc                                                                            <  2:1062324/140‑1 (MQ=255)                                                           cccGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCg                                                                          >  2:42534/1‑140 (MQ=255)                                                                tAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGaa                                                                     <  2:1736593/140‑1 (MQ=255)                                                                     aGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCaa                                                                >  2:2559855/1‑140 (MQ=255)                                                                       tCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCaaaa                                                              <  1:2086012/140‑1 (MQ=255)                                                                       tCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCaaaa                                                              >  1:2547547/1‑140 (MQ=255)                                                                       tCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCaaaa                                                              >  1:566241/1‑140 (MQ=255)                                                                         ccAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATa                                                            >  1:948641/1‑140 (MQ=255)                                                                            aaaTCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATAcgc                                                         >  2:1694775/1‑140 (MQ=255)                                                                                 aaCCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGa                                                    >  2:916612/1‑140 (MQ=255)                                                                                 aaCCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGa                                                    >  2:1524596/1‑140 (MQ=255)                                                                                 aaCCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGa                                                    >  2:1550740/1‑140 (MQ=255)                                                                                 aaCCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGa                                                    >  2:1932255/1‑140 (MQ=255)                                                                                   ccccAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGAca                                                  >  2:700004/1‑140 (MQ=255)                                                                                   ccccAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGAca                                                  >  2:1121370/1‑140 (MQ=255)                                                                                       aGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGAcagcca                                              <  2:2621557/140‑1 (MQ=255)                                                                                        gCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGAcag                                                 <  2:1084171/136‑1 (MQ=255)                                                                                        gCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGAcag                                                 >  1:1084171/1‑136 (MQ=255)                                                                                             ccGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAAc                                        >  1:1150125/1‑140 (MQ=255)                                                                                                ccAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCga                                     >  1:1404530/1‑140 (MQ=255)                                                                                                     tGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTTACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCGaggagg                                <  2:868153/140‑1 (MQ=255)                                                                                                        aaaGCCAATCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCGAGGAGGCCa                             <  1:2160884/140‑1 (MQ=255)                                                                                                               aTCAGCGCCCGCCGGGAGCTGACCTCCAGATGCTGCCCCAGTTCGCCGCTGGCGGAGAGCGCCCAGAACGCCGTCACCTCCCCTTCCGGAGAAGGCAAAATACGCTCCGACAGCCAGCCAACCGGGGAGGCCAGTTGCca                      <  2:338117/140‑1 (MQ=255)                                                                                                               aTCAGCGCCCGCCAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCGAGGAGGCCAGTTGCca                      <  1:2559855/140‑1 (MQ=255)                                                                                                                    cgcccgccAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCACAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCGAGGAGGCCAGTTGCCACACCg                 >  2:2224898/1‑140 (MQ=255)                                                                                                                           cAGGAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCGAGGAGGCCAGTTGCCACACCGCCACGAt          <  1:1694775/140‑1 (MQ=255)                                                                                                                              gAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCCCTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCGAGGAGGCCAGTTGCCACACCGCCACGATGcc       <  2:1819672/140‑1 (MQ=255)                                                                                                                              gAGCTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCGAGGAGGCCAGTTGCCACACCGCCACGATGcc       >  2:530686/1‑140 (MQ=255)                                                                                                                                 cTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCGAGGAGGCCAGTTGCCACACCGCCACGATGCCCAc    <  1:2737782/140‑1 (MQ=255)                                                                                                                                 cTGATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCGAGGAGGCCAGTTGCCACACCGCCACGATGCCCAc    <  2:211300/140‑1 (MQ=255)                                                                                                                                   gATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCGAGGAGGCCAGTTGCCACACCGCCACGATGCCCACCg  >  1:440274/1‑140 (MQ=255)                                                                                                                                   gATCTCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCGAGGAGGCCAGTTGCCACACCGCCACGATGCCCACCg  >  2:2086426/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        GCACGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCGAGGAGGCCAGTTGCCACACCGCCACGATGCCCACCG  >  NC_000913/994511‑994780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑