BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,883	+C	coding (667/687 nt)	rph ←	truncated RNase PH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,879	1	.	C	95.9%	354.9 / 5.4	97	coding (671/687 nt)	rph	truncated RNase PH
Reads supporting (aligned to +/- strand): ref base . (2/2); new base C (35/58); total (37/60)
Fisher's exact test for biased strand distribution p-value = 6.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 104 total aligned reads displayed.

AACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATT‑CCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCT  >  NC_000913/3815746‑3816013

aaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATT‑ccccct                                                                                                                                  >  2:476567/1‑138 (MQ=255)

aaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATT‑ccccct                                                                                                                                  <  2:912270/140‑3 (MQ=255)

  cTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATT‑ccccctcg                                                                                                                                >  2:803701/1‑136 (MQ=255)

   tGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATT‑CCCCTCggg                                                                                                                               >  2:226638/1‑140 (MQ=255)

    ggCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCggg                                                                                                                               >  1:1878406/1‑140 (MQ=255)

     gcgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGc                                                                                                                              <  2:1002570/140‑1 (MQ=255)

     gcgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGc                                                                                                                              <  1:25546/140‑1 (MQ=255)

      cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGcc                                                                                                                             <  1:1284119/140‑1 (MQ=255)

      cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGcc                                                                                                                             <  2:1156629/140‑1 (MQ=255)

       gcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCa                                                                                                                            <  1:641840/140‑1 (MQ=255)

          gATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCagag                                                                                                                         <  2:1845377/140‑1 (MQ=255)

            tatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGcc                                                                                                                       >  1:83536/1‑140 (MQ=255)

             atGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCca                                                                                                                      <  2:1977852/140‑1 (MQ=255)

                   tCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGa                                                                                                                >  2:644924/1‑140 (MQ=255)

                     aTGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCca                                                                                                                      >  2:1884575/1‑132 (MQ=255)

                     aTGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCca                                                                                                                      <  1:1884575/132‑1 (MQ=255)

                     aTGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATg                                                                                                              >  2:113611/1‑140 (MQ=255)

                     aTGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATg                                                                                                              <  1:1119597/140‑1 (MQ=255)

                     aTGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATg                                                                                                              >  2:318154/1‑140 (MQ=255)

                     aTGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAAGCGACTCATCAGTCGCCTTTAAAATCAGTTTTCCAGCGCCCCCTTCTGCGGCGCTACAAAGGATTCG‑ATTCCCCCTCGGGCCCGAGCcaacaaaaag                                                                                                              >  2:1399723/1‑136 (MQ=255)

                      tGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGa                                                                                                             <  2:358346/140‑1 (MQ=255)

                       gCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAg                                                                                                            <  2:1773440/140‑1 (MQ=255)

                         cTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATT‑CCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                         <  1:226638/140‑1 (MQ=255)

                          ttCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                         <  2:840011/140‑1 (MQ=255)

                          ttCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                         <  2:2033461/140‑1 (MQ=255)

                          ttCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                         <  1:1679931/140‑1 (MQ=255)

                          ttCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                         <  1:113611/140‑1 (MQ=255)

                          ttCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACCCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                         <  1:552797/140‑1 (MQ=255)

                          ttCGCTCCCCATCTTCCTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                         <  1:1399723/140‑1 (MQ=255)

                           tCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAg                                                                                                            <  2:1848082/136‑1 (MQ=255)

                           tCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAg                                                                                                            >  1:1848082/1‑136 (MQ=255)

                               tcctcATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCtt                                                                                                    <  1:1316983/140‑1 (MQ=255)

                               tcctcATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCtt                                                                                                    <  2:1382862/140‑1 (MQ=255)

                                cctcATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTGCAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTc                                                                                                   >  2:566818/1‑140 (MQ=255)

                                 ctcATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCa                                                                                                  <  1:1451496/140‑1 (MQ=255)

                                   cATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATg                                                                                                >  2:2164933/1‑140 (MQ=255)

                                        tACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGa                                                                                           <  2:1878406/140‑1 (MQ=255)

                                         aCTTTTCTACAGACAAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGa                                                                                           >  1:157891/1‑140 (MQ=255)

                                         aCTTTTCTACAGAC‑‑AAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAAc                                                                                         >  1:325970/1‑140 (MQ=255)

                                             ttCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCtt                                                                                                    <  1:1384033/126‑1 (MQ=255)

                                             ttCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCtt                                                                                                    >  2:1384033/1‑126 (MQ=255)

                                              tCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCt                                                                                     >  1:1124664/1‑140 (MQ=255)

                                              tCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCt                                                                                     <  1:1709493/140‑1 (MQ=255)

                                                tACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCg                                                                                   >  1:1808839/1‑140 (MQ=255)

                                                tACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCg                                                                                   >  1:2127660/1‑140 (MQ=255)

                                                tACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCg                                                                                   >  1:1497920/1‑140 (MQ=255)

                                                tACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCg                                                                                   >  1:340742/1‑140 (MQ=255)

                                                  cAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGcc                                                                                 <  2:1458972/140‑1 (MQ=255)

                                                   aGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCa                                                                                                  >  1:1370618/1‑122 (MQ=255)

                                                   aGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCa                                                                                                  <  2:1370618/122‑1 (MQ=255)

                                                    gAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCtt                                                                               <  2:1497920/140‑1 (MQ=255)

                                                     aC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTc                                                                              >  1:1794181/1‑140 (MQ=255)

                                                        aaaaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTg                                                                            >  2:1355620/1‑140 (MQ=255)

                                                          aaaaaaGTCGACTCATCAGTCGCCTTAAAATTAAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGcc                                                                          >  2:1892736/1‑140 (MQ=255)

                                                          aaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGcc                                                                          <  2:205892/140‑1 (MQ=255)

                                                             aaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTc                                                                       <  1:644924/140‑1 (MQ=255)

                                                               aGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCACGCCTTCTGCCGTccc                                                                     <  1:476567/140‑1 (MQ=255)

                                                                ggCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCt                                                                                     <  1:165918/123‑1 (MQ=255)

                                                                ggCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCt                                                                                     >  2:165918/1‑123 (MQ=255)

                                                                ggCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTcccc                                                                    >  1:990553/1‑140 (MQ=255)

                                                                  cGACTCATCAGTTACTATAAAAACCAGTATCCCAGCGCCGCCTTCTGCGTCGCTACAATTGATGCG‑ATTCCCCCGCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAAAGGAAAGCCTTCTGCTGTCCCCTg                                                                  <  2:1508150/140‑1 (MQ=255)

                                                                  cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                  <  2:495568/140‑1 (MQ=255)

                                                                  cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                  <  2:325970/140‑1 (MQ=255)

                                                                  cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                  <  2:157891/140‑1 (MQ=255)

                                                                  cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                  <  2:83536/140‑1 (MQ=255)

                                                                  cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                  <  1:2045679/140‑1 (MQ=255)

                                                                  cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                  <  1:803701/140‑1 (MQ=255)

                                                                  cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                  <  2:1124664/140‑1 (MQ=255)

                                                                         tcaGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCa                                                           <  1:1449210/140‑1 (MQ=255)

                                                                          caGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCaa                                                          <  1:331993/140‑1 (MQ=255)

                                                                            gTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatg                                                        >  1:1790330/1‑140 (MQ=255)

                                                                              cGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatgat                                                      <  1:2164933/140‑1 (MQ=255)

                                                                               gCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                  <  2:2026217/127‑1 (MQ=255)

                                                                               gCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatgatg                                                     <  2:1536060/140‑1 (MQ=255)

                                                                               gCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatgatg                                                     >  1:295979/1‑140 (MQ=255)

                                                                               gCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatgatg                                                     >  1:632889/1‑140 (MQ=255)

                                                                                 cTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgcg                                                   <  2:1507205/140‑1 (MQ=255)

                                                                                  ttAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgcgc                                                  >  1:1152767/1‑140 (MQ=255)

                                                                                         tCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCtt                                           <  2:1790330/140‑1 (MQ=255)

                                                                                           aGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCg                                         <  2:1794181/140‑1 (MQ=255)

                                                                                            gTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCgg                                        >  1:579817/1‑140 (MQ=255)

                                                                                             tttGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatg                                                        <  2:439153/123‑1 (MQ=255)

                                                                                             tttGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatg                                                        >  1:439153/1‑123 (MQ=255)

                                                                                             tttGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGt                                       >  1:27235/1‑140 (MQ=255)

                                                                                              ttGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtc                                      >  1:1877363/1‑140 (MQ=255)

                                                                                               tGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtca                                     <  2:1215673/140‑1 (MQ=255)

                                                                                                gCCAGCGCCGCCTTCTGCGTCGCTGCAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcat                                    <  1:566818/140‑1 (MQ=255)

                                                                                                gCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcat                                    <  1:340794/140‑1 (MQ=255)

                                                                                                gCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcat                                    <  1:237619/140‑1 (MQ=255)

                                                                                                  cAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcatca                                  >  1:1423986/1‑140 (MQ=255)

                                                                                                  cAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcatca                                  <  2:295979/140‑1 (MQ=255)

                                                                                                    gcgcCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACt                                <  2:340742/140‑1 (MQ=255)

                                                                                                    gcgcCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACt                                <  1:758916/140‑1 (MQ=255)

                                                                                                      gccgccTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTAc                              >  1:711572/1‑140 (MQ=255)

                                                                                                               tGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGt                                       >  2:861982/1‑122 (MQ=255)

                                                                                                                    cGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGAGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGtct                >  2:746381/1‑140 (MQ=255)

                                                                                                                      cTACAATGGATTCGAATC‑CCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTc                          <  1:1934766/128‑1 (MQ=255)

                                                                                                                      cTACAATGGATTCGAATC‑CCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTc                          >  2:1934766/1‑128 (MQ=255)

                                                                                                                       tACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTg             <  2:2107263/140‑1 (MQ=255)

                                                                                                                                  cg‑attcCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCt  <  1:2186401/140‑1 (MQ=255)

AACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATT‑CCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCT  >  NC_000913/3815746‑3816013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`