Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,556,207	0	T	C	28.8%	108.2 / 16.3	59	S215G (AGT→GGT) ‡	rtcA	RNA 3'‑terminal phosphate cyclase
Reads supporting (aligned to +/- strand):  ref base T (38/4);  new base C (0/17);  total (38/21)
Fisher's exact test for biased strand distribution p-value = 2.16e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGT  >  NC_000913/3556070‑3556345                                                                                                                                           |                                                                                                                                           cTCGGCACTGACGCGCTTTACACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTcc                                                                                                                                          >  1:1409409/1‑140 (MQ=255)    ggCATTGCCGCGCTTTTCCCCCACAAAAAAAAGGCGTTCGGTGATATTTTCTTTTCCGCCTTCAAGCGAGACGGTATTCCCCGGCCCCGGGCCGCGGGGCAGTTTATGAATTTTTTGTTCATGC‑GGGAAAAA‑cccccccg                                                                                                                                      <  2:172673/140‑3 (MQ=255)    ggCACTGCCGCGTTTTCCCCCAACAAAAAAAAAGCGTGCGGTGATATTTACCCTTTCGACTTCAAGCGAGACTGTATCCCCCGCCCCCTGGTCCCGCGGAGGTTTATAAATATTCTTTTCTTGCAGGGAAAAA‑CCCCCCg                                                                                                                                       <  2:82345/140‑1 (MQ=255)     gCACTGACCCGCTTTCCCCCGACAAAAAAAAGGCGTTGGGTAATTTTTTCCTTTTCGACTAAAAGCGAGACGATATTCCCCGGCCCCTGGTCGCGCGGCAGTTTATGAATATTCTGTTCAGGCAGGAAAAAC‑CCCCCCGc                                                                                                                                      <  2:1333773/140‑1 (MQ=255)           aCGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAgtgt                                                                                                                                >  1:1405950/1‑140 (MQ=255)           aCGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAgtgt                                                                                                                                >  1:1660201/1‑140 (MQ=255)             gcgtTTTTCCCCGACGAAAAAAAAGCGTTGGGTGATTTTTTCCCTTTCGACTTCAAGCGAGACGGTATTCCCCGGCCCCTGGTCGCGCGGCAGTTTATGAATTTTCTGTTCATGCAGGAAAAAC‑CTCCCCGCCAGTGTAg                                                                                                                              <  2:260899/140‑1 (MQ=255)                cTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGa                                                                                                                           >  1:1667992/1‑140 (MQ=255)                     cACCGCCGAAAAAAAGGCGTCGGGTGTTTTTTTCTTTTGCGACTTCAAGGGAGTCGGTTTTCCCCGCCCCCGGGGCGCGGGGCAGGTTATAAATATTGTTTTCTGGGAGGAAAAAC‑CTCGCCGCCAGTGTAGCGATTTCa                                                                                                                      <  2:282132/140‑1 (MQ=255)                       ccGACGAAAAAAAAGCGTTCGGTGATTTTTTCACTTGCGACTTCAAGCGAGACGGTATTCCCCGGCCCCTGGTCGCGGGGCAGTTTATGAATTTTCTGTTCATGAAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACg                                                                                                                    <  2:434837/140‑1 (MQ=255)                       ccGACAAAAAAAAAGCGTTCGGTGTTTTTTTCTCTTTCGACTTAAAGCGAGACGTTATTCCCCGCCCCCTGGCCGCGCGGCAGTTTATGAATTTTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACg                                                                                                                    <  2:591416/140‑1 (MQ=255)                         gacgacAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCt                                                                                                                  >  1:591416/1‑140 (MQ=255)                            aaaaaaaagGCGTTCGGTAATTTTTTCCCTTCCGACTTCAAGCGAGACGTTATTCCCCGCCCCCGGGCCGCGCGGCAGTTTATGAATATTCTGTTCAGGAAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAg                                                                                                               <  2:523037/137‑1 (MQ=255)                             acAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGc                                                                                                              >  1:1916997/1‑140 (MQ=255)                             acAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGc                                                                                                              >  1:844711/1‑140 (MQ=255)                             acAAAAAAGCGTTCGGTGATATTTTCACTGTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGc                                                                                                              >  1:1881053/1‑140 (MQ=255)                               aaaaaaGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCaa                                                                                                            >  1:2123103/1‑140 (MQ=255)                               aaaaaaGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCaa                                                                                                            >  1:1599231/1‑140 (MQ=255)                                       gTTGGGTTATTTTTTCTTTTTCGACTACAAGCGAGCGGTTATTCCCCGCCCCCTGGCCGCGGGGCGGTTTATAAATTTTTTTTTCAGGCGGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGgcgc                                                                                                    <  2:1667992/140‑1 (MQ=255)                                        ttCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGcgcg                                                                                                   >  2:2043320/1‑140 (MQ=255)                                        ttCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGcgcg                                                                                                   >  1:1468263/1‑140 (MQ=255)                                             taattttttCCCTTTCGCCTCAAAGCGAGACGGTTTTCCCCGCCCCTTGGTCGCGCGGCAGGTTATAAATATTCTTTTCAGGCAGGGAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGcaca                                                                                              <  2:908368/135‑1 (MQ=255)                                             tGATATTTTCCCTTCCGACTTCAAGCGAGACGTTATTCCCCGCCCCCGGGTCGCGCGGCAGTTTATAAATTTTCTGTTCATGCGGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGcaca                                                                                              <  2:395353/140‑1 (MQ=255)                                                ttttttCATTTTCGACTTCAAGCGAGCCGTTATTCCCCGGCCCTGGGCCGCGGGGCAGGTTATGAATTTTCTTTTCATGAAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCa                                                                                           <  2:2034497/138‑1 (MQ=255)                                                 tttttCCCTTTCGATTTCAAGCGAGACGTTTTTCCCGGGCCCCGGGCCGCGGGGAGGTTTATAAATTTTCTGTTCATGAAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAg                                                                                          <  2:104093/139‑1 (MQ=255)                                                  ttttCTCTTTCGACTTCAAGCGAGACGTTATTCCCCGGCCCCTGGTCGCGCGGCAGTTTATAAATATTCTGTTCATG‑AGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGc                                                                                         <  2:525984/140‑1 (MQ=255)                                                     tCACTTTCGACTTCAAGCGAGCGGTTTTTCCCCGCCCCCTGGCCGCGCGGCAGGTTATGAATTTTCTGTTCATGCGGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTaa                                                                                      <  2:649230/140‑1 (MQ=255)                                                       aCTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATa                                                                                    >  1:585784/1‑140 (MQ=255)                                                          ttCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGaa                                                                                 >  1:1074320/1‑140 (MQ=255)                                                          ttCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGaa                                                                                 >  1:80080/1‑140 (MQ=255)                                                          ttCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGaa                                                                                 >  2:19306/1‑140 (MQ=255)                                                           tCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAAc                                                                                >  2:2086139/1‑140 (MQ=255)                                                                          gcGGTTTTCCCCGGCCCCGGGTCGCGCGGAGGGTTATGAATTTTCTTTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTg                                                                 <  2:1169418/139‑1 (MQ=255)                                                                            ggTATTCCCCGCCCCCTGGCCGCGCGGCAGGTTATGAATTTTTTTTTCATGCGGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGca                                                               <  1:2113829/140‑1 (MQ=255)                                                                              tatcccccGCCCCCGGGTCGCGGGGAAGGTTATAAATATTCTGTTCATGCAGGGAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGcaca                                                             <  2:1074320/135‑1 (MQ=255)                                                                               aTTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACaa                                                            >  2:617642/1‑140 (MQ=255)                                                                                        ccccTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCgcgc                                                   >  1:370435/1‑140 (MQ=255)                                                                                        ccccTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcgc                                                   >  2:132666/1‑140 (MQ=255)                                                                                        ccccTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcgc                                                   >  1:909201/1‑140 (MQ=255)                                                                                        ccccTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcgc                                                   >  1:939629/1‑140 (MQ=255)                                                                                           cTGGTCGCGCGGCAGTTTATGAATTTTTTGTTCATGCGGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCt                                                <  1:1753007/140‑1 (MQ=255)                                                                                             ggTCGCGGGGCGGTTTATAAATTTTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCg                                              <  1:1491562/140‑1 (MQ=255)                                                                                               tCGCGCGGCAGTTTATGAATTTTCTTTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGcc                                            <  1:653862/140‑1 (MQ=255)                                                                                                ggcgcgGAAGGTTATAAATATTCTGTTAATGGAGAGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTT‑‑CGCGCTCGCCAAg                                         <  2:370435/139‑1 (MQ=255)                                                                                                  ggcgGCAGTTTATAAATTTTCTGTTCATGAAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAg                                         <  2:1405950/139‑1 (MQ=255)                                                                                                  cgcgGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCTACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAg                                         >  2:581830/1‑140 (MQ=255)                                                                                                  cgcgGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAg                                         >  1:2187587/1‑140 (MQ=255)                                                                                                       cAGTTTATGAATTTTTTGTTCATGAAGAAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCa                                    <  2:909201/140‑1 (MQ=255)                                                                                                              taaaTTTTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGtt                             <  1:1661652/138‑1 (MQ=255)                                                                                                                    ttCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa                       >  2:1816360/1‑140 (MQ=255)                                                                                                                     tCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAAcctg                      >  1:1445698/1‑137 (MQ=255)                                                                                                                     tCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGAt                      >  1:1129280/1‑140 (MQ=255)                                                                                                                     tCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGAt                      >  1:1737325/1‑140 (MQ=255)                                                                                                                        gTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGcc                   >  2:860754/1‑140 (MQ=255)                                                                                                                         ttCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa                  >  1:37279/1‑140 (MQ=255)                                                                                                                         ttCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa                  >  1:2048492/1‑140 (MQ=255)                                                                                                                         ttCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa                  >  1:1880038/1‑140 (MQ=255)                                                                                                                         ttCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa                  >  1:1026262/1‑140 (MQ=255)                                                                                                                         ttCAGGAAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa                  <  2:2073426/140‑1 (MQ=255)                                                                                                                          tCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCAc                 >  2:173752/1‑140 (MQ=255)                                                                                                                            aTGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACgg               >  2:472442/1‑140 (MQ=255)                                                                                                                            aTGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACgg               >  2:82833/1‑140 (MQ=255)                                                                                                                            aTGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACgg               >  2:989816/1‑140 (MQ=255)                                                                                                                                   gAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGAc        >  1:67292/1‑140 (MQ=255)                                                                                                                                     aaaa‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACtt      <  2:1880038/140‑1 (MQ=255)                                                                                                                                          cTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGt  <  2:2123103/140‑1 (MQ=255)                                                                                                                                          cTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGt  <  2:1599231/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                           CTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGT  >  NC_000913/3556070‑3556345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑