BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,021,557	G→A	L18L (CTG→CTA)	fliO →	flagellar biosynthesis protein FliO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,021,557	0	G	A	100.0%	412.1 / NA	119	L18L (CTG→CTA)	fliO	flagellar biosynthesis protein FliO
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (66/53); total (66/53)

Only 100 of 126 total aligned reads displayed.

TGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGG  >  NC_000913/2021422‑2021691

    cGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGt                                                                                                                                <  2:1130841/140‑1 (MQ=255)

    cGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGt                                                                                                                                <  2:745901/140‑1 (MQ=255)

       tGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAg                                                                                                                             >  2:709168/1‑140 (MQ=255)

            aTAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAgcggcg                                                                                                                        <  2:1380220/140‑1 (MQ=255)

                     gCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCg                                                                                                               <  2:1272628/140‑1 (MQ=255)

                      cGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGc                                                                                                              <  1:321566/140‑1 (MQ=255)

                        tGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCAACGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGccc                                                                                                            >  2:1067677/1‑139 (MQ=255)

                        tGCGGATCACCGATATAATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCa                                                                                                            >  2:1411690/1‑140 (MQ=255)

                         gCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCat                                                                                                           >  1:406237/1‑140 (MQ=255)

                          cGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGc                                                                                                              >  1:2095500/1‑136 (MQ=255)

                          cGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGc                                                                                                              >  2:2143549/1‑136 (MQ=255)

                          cGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGc                                                                                                              <  2:2095500/136‑1 (MQ=255)

                          cGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGc                                                                                                              <  1:2143549/136‑1 (MQ=255)

                          cGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGc                                                                                                              >  2:1082855/1‑136 (MQ=255)

                          cGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCatt                                                                                                          >  2:869069/1‑140 (MQ=255)

                          cGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCatt                                                                                                          >  1:1532857/1‑140 (MQ=255)

                          cGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCatt                                                                                                          >  2:515301/1‑140 (MQ=255)

                           ggATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCatta                                                                                                         <  2:1532857/140‑1 (MQ=255)

                            gATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCattat                                                                                                        <  1:1230276/140‑1 (MQ=255)

                                aCCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGcc                                                                                                    <  1:327552/140‑1 (MQ=255)

                                aCCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGcc                                                                                                    <  2:1408345/140‑1 (MQ=255)

                                     tatCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGAt                                                                                               <  2:1999468/140‑1 (MQ=255)

                                       tCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATcc                                                                                             >  1:1290515/1‑140 (MQ=255)

                                         aTTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTc                                                                                           >  1:954933/1‑140 (MQ=255)

                                         aTTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTc                                                                                           >  2:2132712/1‑140 (MQ=255)

                                          ttACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCg                                                                                          >  2:644623/1‑140 (MQ=255)

                                          ttACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCg                                                                                          <  1:1997237/140‑1 (MQ=255)

                                           tACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCgc                                                                                         <  2:15167/140‑1 (MQ=255)

                                               ccGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTTCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCgctgct                                                                                     >  1:588640/1‑140 (MQ=255)

                                               ccGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCgctgct                                                                                     >  1:1837265/1‑140 (MQ=255)

                                                cGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCTACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGctgctg                                                                                    >  2:234941/1‑140 (MQ=255)

                                                cGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGctgctg                                                                                    >  2:559935/1‑140 (MQ=255)

                                                cGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCATTTATTGCCCTGATCCTCGctgctg                                                                                    >  1:149423/1‑140 (MQ=255)

                                                cGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAAGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGctgctg                                                                                    >  1:1043660/1‑140 (MQ=255)

                                                 gTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCtgctgc                                                                                   >  2:126609/1‑140 (MQ=255)

                                                 gTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTCTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCtgctgc                                                                                   <  2:699792/140‑1 (MQ=255)

                                                  tCTGAGCGAATGCGCCGCCTGTGCTGTTAGTGATGAATAAACACGCTACTGAGCAATCTTCCGCGCCGGTTTCTGATGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGcc                                                                                  >  2:2219545/1‑140 (MQ=255)

                                                  tCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGTTGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGcc                                                                                  >  1:1713648/1‑140 (MQ=255)

                                                  tCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGcc                                                                                  >  1:960017/1‑140 (MQ=255)

                                                  tCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGcc                                                                                  >  2:2190910/1‑140 (MQ=255)

                                                  tCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGcc                                                                                  >  2:171152/1‑140 (MQ=255)

                                                  tCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGcc                                                                                  >  1:423899/1‑140 (MQ=255)

                                                  tCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGcc                                                                                  >  1:1329349/1‑140 (MQ=255)

                                                  tCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGcc                                                                                  >  2:70734/1‑140 (MQ=255)

                                                  tCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGcc                                                                                  >  1:1802062/1‑140 (MQ=255)

                                                  tCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGcc                                                                                  >  1:1850936/1‑140 (MQ=255)

                                                  tCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGcc                                                                                  >  1:1542619/1‑140 (MQ=255)

                                                  tCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGcc                                                                                  >  1:308480/1‑140 (MQ=255)

                                                  tCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGcc                                                                                  >  1:472735/1‑140 (MQ=255)

                                                  tCTGAGCGAATGCGCCACCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGcc                                                                                  >  2:1957684/1‑140 (MQ=255)

                                                    tGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCctg                                                                                <  1:328131/140‑1 (MQ=255)

                                                    tGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCctg                                                                                <  1:1590009/140‑1 (MQ=255)

                                                     gAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCctgg                                                                               >  1:1733949/1‑140 (MQ=255)

                                                            tGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTaa                                                                        >  2:1860006/1‑140 (MQ=255)

                                                            tGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTaa                                                                        <  2:88898/140‑1 (MQ=255)

                                                            tGCCCCGCCCGGGCCGGTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTaa                                                                        <  2:2161809/140‑1 (MQ=255)

                                                              cgccgcCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTaaaa                                                                      <  2:1618717/140‑1 (MQ=255)

                                                              cgccgcCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTaaaa                                                                      <  1:1575116/140‑1 (MQ=255)

                                                                         ccGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGAtt                                                           <  2:1837265/140‑1 (MQ=255)

                                                                           gTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTg                                                         <  1:456365/140‑1 (MQ=255)

                                                                             tAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGcc                                                       >  1:731071/1‑140 (MQ=255)

                                                                                  atgaATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCTACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTaa                                                  <  1:234941/140‑1 (MQ=255)

                                                                                   tgaATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTaaa                                                 <  1:102718/140‑1 (MQ=255)

                                                                                       tAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCa                                             <  2:1001859/140‑1 (MQ=255)

                                                                                       tAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCa                                             <  1:849679/140‑1 (MQ=255)

                                                                                       tAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCa                                             <  1:1341763/140‑1 (MQ=255)

                                                                                       tAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCa                                             >  2:213919/1‑140 (MQ=255)

                                                                                       tAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCa                                             <  1:869069/140‑1 (MQ=255)

                                                                                       tAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCa                                             <  2:1802062/140‑1 (MQ=255)

                                                                                       tAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCCGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCa                                             <  1:1123403/140‑1 (MQ=255)

                                                                                        aaCCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCAc                                            <  1:1574612/140‑1 (MQ=255)

                                                                                         aCCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGTGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACt                                           >  1:1071025/1‑140 (MQ=255)

                                                                                         aCCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACt                                           >  2:2043668/1‑140 (MQ=255)

                                                                                         aCCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACt                                           <  2:588640/140‑1 (MQ=255)

                                                                                          ccACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCa                                             <  1:1255360/137‑1 (MQ=255)

                                                                                          ccACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCa                                             >  2:1255360/1‑137 (MQ=255)

                                                                                          ccACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTg                                          >  1:1730034/1‑140 (MQ=255)

                                                                                            aCGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGc                                        >  2:176556/1‑140 (MQ=255)

                                                                                             cGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCa                                             >  2:1568429/1‑134 (MQ=255)

                                                                                             cGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCa                                             <  1:1568429/134‑1 (MQ=255)

                                                                                             cGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCg                                       >  1:925064/1‑140 (MQ=255)

                                                                                               cTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGtt                                     >  1:1364857/1‑140 (MQ=255)

                                                                                                 aCTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTaa                                   <  2:1406810/140‑1 (MQ=255)

                                                                                                  cTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGATGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAAc                                  <  1:1625754/140‑1 (MQ=255)

                                                                                                     tgCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGt                               <  2:1850936/140‑1 (MQ=255)

                                                                                                     tgCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGt                               >  2:1282229/1‑140 (MQ=255)

                                                                                                     tgCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGt                               >  1:2158152/1‑140 (MQ=255)

                                                                                                       cAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCt                             >  1:1700075/1‑140 (MQ=255)

                                                                                                       cAATCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCt                             >  1:2051539/1‑140 (MQ=255)

                                                                                                          tCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGaa                          >  1:1358585/1‑140 (MQ=255)

                                                                                                          tCTTCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGaa                          >  1:675690/1‑140 (MQ=255)

                                                                                                            ttCCGCGCCGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGaaaa                        >  1:1056113/1‑140 (MQ=255)

                                                                                                                   ccGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCATTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAgcgc                 <  1:2219545/140‑1 (MQ=255)

                                                                                                                   ccGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAgcgc                 <  2:2000389/140‑1 (MQ=255)

                                                                                                                   ccGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAgcgc                 <  1:70734/140‑1 (MQ=255)

                                                                                                                   ccGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAgcgc                 <  2:960017/140‑1 (MQ=255)

                                                                                                                   ccGGTTTCTGCTGCGCCACTACTGCAGGTGAGCGGAGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAgcgc                 <  2:1056113/140‑1 (MQ=255)

                                                                                                                     ggTTTCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCa               <  1:2190910/140‑1 (MQ=255)

                                                                                                                        ttCTGCTGCGCCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTg            <  2:1542619/140‑1 (MQ=255)

                                                                                                                                cgcCACTACTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTg    >  1:688846/1‑140 (MQ=255)

TGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGTCTGAAAATTAGCGCCAGTGCTTCACTGGG  >  NC_000913/2021422‑2021691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`