BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,624,927	G→A	L628L (CTG→CTA)	ppk →	polyphosphate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,624,927	0	G	A	99.4%	542.0 / NA	156	L628L (CTG→CTA)	ppk	polyphosphate kinase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (86/69); minor base T (0/1); total (86/70)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 159 total aligned reads displayed.

ATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTA  >  NC_000913/2624790‑2625056

aTCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCgctact                                                                                                                                 <  1:95646/140‑1 (MQ=255)

 tCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTc                                                                                                                                >  1:1829379/1‑140 (MQ=255)

 tCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTc                                                                                                                                >  2:2103756/1‑140 (MQ=255)

   aGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTATACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGa                                                                                                                              >  2:1636401/1‑140 (MQ=255)

     tATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATc                                                                                                                            <  1:139029/140‑1 (MQ=255)

            gACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCt                                                                                                                     >  1:736367/1‑140 (MQ=255)

             aCCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGTGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTg                                                                                                                    >  2:1275160/1‑140 (MQ=255)

             aCCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTg                                                                                                                    >  2:86783/1‑140 (MQ=255)

             aCCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTg                                                                                                                    <  2:118924/140‑1 (MQ=255)

             aCCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTg                                                                                                                    >  2:1695339/1‑140 (MQ=255)

               cGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGaa                                                                                                                  >  2:2383576/1‑140 (MQ=255)

               cGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGaa                                                                                                                  >  1:1233722/1‑140 (MQ=255)

                gTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAag                                                                                                                 <  2:418210/140‑1 (MQ=255)

                 ttACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAagc                                                                                                                >  2:1322613/1‑140 (MQ=255)

                 ttACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAagc                                                                                                                >  2:717470/1‑140 (MQ=255)

                 ttACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAagc                                                                                                                >  2:853052/1‑140 (MQ=255)

                  tACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTATACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAagca                                                                                                               <  1:1636401/140‑1 (MQ=255)

                    ccTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGTATGACGCGGAATATTGATTATCGTATTGAAGTGGCGACCCCGCTACTCGATCCGCGCCTGAagcagc                                                                                                             <  2:1867567/140‑1 (MQ=255)

                    ccTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAagcagc                                                                                                             <  2:536303/140‑1 (MQ=255)

                    ccTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAagcagc                                                                                                             <  1:295788/140‑1 (MQ=255)

                    ccTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAagcagc                                                                                                             <  2:553329/140‑1 (MQ=255)

                    ccTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAagcagc                                                                                                             <  2:736367/140‑1 (MQ=255)

                         aaCATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTa                                                                                                        <  1:1186796/140‑1 (MQ=255)

                           cATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACt                                                                                                      >  1:1998407/1‑140 (MQ=255)

                             tGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTgg                                                                                                    <  1:1695339/140‑1 (MQ=255)

                              gACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGa                                                                                                   >  2:923521/1‑140 (MQ=255)

                              gACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGa                                                                                                   >  1:1778494/1‑140 (MQ=255)

                              gACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGGACTGGa                                                                                                   >  2:2286849/1‑140 (MQ=255)

                              gACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCCACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTa                                                                                                        >  2:732604/1‑135 (MQ=255)

                               aCCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGaa                                                                                                                  <  1:2367342/124‑1 (MQ=255)

                               aCCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGaa                                                                                                                  >  2:2367342/1‑124 (MQ=255)

                               aCCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAagcagc                                                                                                             >  2:1358906/1‑129 (MQ=255)

                               aCCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAagcagc                                                                                                             <  2:617542/129‑1 (MQ=255)

                               aCCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGAc                                                                                                  <  1:111649/140‑1 (MQ=255)

                               aCCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGGCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGGTTATCGTATTGAAGGGGCGACGCCGCTACTCGATCCGCGCCTGAagcagc                                                                                                             >  1:617542/1‑129 (MQ=255)

                                   ggTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAagcagc                                                                                                             <  1:736027/125‑1 (MQ=255)

                                    gTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGAcatcat                                                                                             <  2:1624986/140‑1 (MQ=255)

                                    gTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGAcatcat                                                                                             <  1:717470/140‑1 (MQ=255)

                                    gTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGAcatcat                                                                                             <  1:1548198/140‑1 (MQ=255)

                                      ttATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCg                                                                                           <  2:1962852/140‑1 (MQ=255)

                                        atatTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGAc                                                                                         >  2:1226135/1‑140 (MQ=255)

                                        atatTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGAc                                                                                         <  2:1091013/140‑1 (MQ=255)

                                         tatTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACa                                                                                        >  1:1494237/1‑140 (MQ=255)

                                           tttttGAAAATGGCGGTGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACata                                                                                      <  2:1024534/140‑1 (MQ=255)

                                           tttttGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACata                                                                                      <  1:715126/140‑1 (MQ=255)

                                           tttttGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACata                                                                                      <  1:1062883/140‑1 (MQ=255)

                                            ttttGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACatat                                                                                     <  2:1494237/140‑1 (MQ=255)

                                              ttGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACata                                                                                      <  2:1724450/137‑1 (MQ=255)

                                              ttGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTg                                                                                   >  1:138708/1‑140 (MQ=255)

                                              ttGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTg                                                                                   <  2:266649/140‑1 (MQ=255)

                                              ttGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTg                                                                                   >  1:1138908/1‑140 (MQ=255)

                                              ttGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTg                                                                                   >  1:1622799/1‑140 (MQ=255)

                                              ttGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTg                                                                                   >  1:2001895/1‑140 (MQ=255)

                                                            gATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGt                                                                     <  1:457220/140‑1 (MQ=255)

                                                            gATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGt                                                                     <  1:1780926/140‑1 (MQ=255)

                                                             aTAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTc                                                                    <  1:1322613/140‑1 (MQ=255)

                                                                aaaaGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCaaa                                                                 <  1:2352008/140‑1 (MQ=255)

                                                                aaaaGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCaaa                                                                 <  1:616883/140‑1 (MQ=255)

                                                                aaaaGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCaaa                                                                 <  1:1570585/140‑1 (MQ=255)

                                                                 aaaGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATTCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAg                                                                <  2:2156360/140‑1 (MQ=255)

                                                                 aaaGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAg                                                                <  2:177726/140‑1 (MQ=255)

                                                                  aaGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGc                                                               <  2:1829379/140‑1 (MQ=255)

                                                                        tACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTta                                                         >  1:1870232/1‑140 (MQ=255)

                                                                           cTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTatat                                                      <  2:1506101/140‑1 (MQ=255)

                                                                           cTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTatat                                                      <  2:612358/140‑1 (MQ=255)

                                                                             ttcttcCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCg                                                    >  2:2312330/1‑140 (MQ=255)

                                                                               cttcCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGAt                                                  >  1:689882/1‑140 (MQ=255)

                                                                               cttcCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGAt                                                  >  1:2389258/1‑140 (MQ=255)

                                                                               cttcCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGAt                                                  >  2:489802/1‑140 (MQ=255)

                                                                                    gccgACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGa                                             >  1:1695346/1‑140 (MQ=255)

                                                                                        aCTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTc                                         >  2:768888/1‑140 (MQ=255)

                                                                                          tGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAg                                       >  1:844096/1‑140 (MQ=255)

                                                                                           ggATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGt                                      <  2:689882/140‑1 (MQ=255)

                                                                                             aTGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTaa                                    <  2:1695346/140‑1 (MQ=255)

                                                                                                 cgcgCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGc                                >  1:281500/1‑140 (MQ=255)

                                                                                                      aaTATTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGt                           >  2:32713/1‑140 (MQ=255)

                                                                                                       atatTGATTATCGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGtt                          <  2:183751/140‑1 (MQ=255)

                                                                                                                  cGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCaa               >  2:544251/1‑140 (MQ=255)

                                                                                                                  cGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCaa               >  2:1117409/1‑140 (MQ=255)

                                                                                                                  cGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCaa               >  2:3910/1‑140 (MQ=255)

                                                                                                                  cGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCaa               >  2:175378/1‑140 (MQ=255)

                                                                                                                  cGTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCaa               >  1:901729/1‑140 (MQ=255)

                                                                                                                   gTATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAAt              <  1:853052/140‑1 (MQ=255)

                                                                                                                    tATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATc             <  2:281500/140‑1 (MQ=255)

                                                                                                                    tATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATc             >  1:1301702/1‑140 (MQ=255)

                                                                                                                    tATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATc             >  1:1616679/1‑140 (MQ=255)

                                                                                                                    tATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATc             >  2:687220/1‑140 (MQ=255)

                                                                                                                    tATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATc             >  1:423338/1‑140 (MQ=255)

                                                                                                                    tATTGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATc             >  2:2315556/1‑140 (MQ=255)

                                                                                                                      ttGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATcgc           >  1:256655/1‑140 (MQ=255)

                                                                                                                      ttGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATcgc           >  1:2282132/1‑140 (MQ=255)

                                                                                                                      ttGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATcgc           >  2:406513/1‑140 (MQ=255)

                                                                                                                       tGAAGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATcgcc          >  2:1067226/1‑140 (MQ=255)

                                                                                                                         aaGTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATcgccg         <  2:366943/139‑1 (MQ=255)

                                                                                                                           gTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCaa      <  1:406513/140‑1 (MQ=255)

                                                                                                                           gTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCaa      <  2:786629/140‑1 (MQ=255)

                                                                                                                           gTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCaa      >  1:1141685/1‑140 (MQ=255)

                                                                                                                           gTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCa       >  2:1903445/1‑139 (MQ=255)

                                                                                                                           gTGGCGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCa       <  1:1903445/139‑1 (MQ=255)

                                                                                                                               cGACGCCGCTACTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTa  <  1:1067226/140‑1 (MQ=255)

ATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTA  >  NC_000913/2624790‑2625056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`