BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,223,159	G→T	R176L (CGC→CTC)	ebgA →	evolved beta‑D‑galactosidase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,223,159	0	G	T	100.0%	390.8 / NA	109	R176L (CGC→CTC)	ebgA	evolved beta‑D‑galactosidase subunit alpha
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (55/54); total (55/54)

Only 100 of 112 total aligned reads displayed.

TAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGAC  >  NC_000913/3223022‑3223295

tAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGctcg                                                                                                                                        >  1:1018315/1‑140 (MQ=255)

        aCGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAg                                                                                                                                >  2:1272642/1‑140 (MQ=255)

        aCGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAg                                                                                                                                >  2:1656606/1‑140 (MQ=255)

        aCGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAg                                                                                                                                >  1:526096/1‑140 (MQ=255)

            cGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTggg                                                                                                                            >  2:649758/1‑140 (MQ=255)

            cGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTggg                                                                                                                            >  1:672930/1‑140 (MQ=255)

            cGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTggg                                                                                                                            >  1:1551547/1‑140 (MQ=255)

            cGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTggg                                                                                                                            <  2:636580/140‑1 (MQ=255)

            cGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTACTCGTGATGCAGTggg                                                                                                                            <  1:593873/140‑1 (MQ=255)

             gTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGc                                                                                                                           <  1:385259/140‑1 (MQ=255)

              tCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCg                                                                                                                          <  1:237340/140‑1 (MQ=255)

              tCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCg                                                                                                                          <  1:2351207/140‑1 (MQ=255)

              tCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCg                                                                                                                          <  2:244137/140‑1 (MQ=255)

                        ttttGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTggg                                                                                                                            <  2:24616/128‑1 (MQ=255)

                        ttttGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTggg                                                                                                                            >  1:24616/1‑128 (MQ=255)

                        ttttGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTctacct                                                                                                                <  2:1884884/140‑1 (MQ=255)

                         tttGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTctaccta                                                                                                               <  2:123558/140‑1 (MQ=255)

                              aGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTgg                                                                                                          <  1:1541007/140‑1 (MQ=255)

                               gTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGGTGCAGTGGGCGGACTCTACCTACGTGGa                                                                                                         >  2:209813/1‑140 (MQ=255)

                               gTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGa                                                                                                         >  2:415303/1‑140 (MQ=255)

                               gTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGa                                                                                                         >  2:1116550/1‑140 (MQ=255)

                                tCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGaa                                                                                                        >  2:567813/1‑140 (MQ=255)

                                 cTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAg                                                                                                       <  1:754188/140‑1 (MQ=255)

                                    tGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGAcc                                                                                                    >  2:1220166/1‑140 (MQ=255)

                                       tAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAgg                                                                                                 <  1:762233/140‑1 (MQ=255)

                                         aCGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGat                                                                                               <  1:544500/140‑1 (MQ=255)

                                         aCGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGat                                                                                               <  1:649758/140‑1 (MQ=255)

                                          cGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGata                                                                                              >  2:1492663/1‑140 (MQ=255)

                                              cAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGAcc                                                                                                    <  2:724295/130‑1 (MQ=255)

                                              cAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGAcc                                                                                                    >  1:724295/1‑130 (MQ=255)

                                              cAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATAtgtg                                                                                          >  2:485611/1‑140 (MQ=255)

                                              cAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATAtgtg                                                                                          >  2:1086098/1‑140 (MQ=255)

                                              cAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGT‑‑TGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATgtggt                                                                                        >  1:2285310/1‑140 (MQ=255)

                                                gTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATgtggt                                                                                        >  2:3402/1‑140 (MQ=255)

                                                gTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATgtggt                                                                                        >  2:1400714/1‑140 (MQ=255)

                                                 tATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATgtggtg                                                                                       <  2:611530/140‑1 (MQ=255)

                                                      gggTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAg                                                                                  <  2:2346048/140‑1 (MQ=255)

                                                       ggTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGc                                                                                 >  2:1975203/1‑140 (MQ=255)

                                                         tttCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCgg                                                                               >  2:602955/1‑140 (MQ=255)

                                                         tttCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCgg                                                                               >  1:537513/1‑140 (MQ=255)

                                                          ttCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCggg                                                                              <  2:1800604/140‑1 (MQ=255)

                                                           tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCgggg                                                                             >  2:1742045/1‑140 (MQ=255)

                                                           tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCgggg                                                                             >  2:1177508/1‑140 (MQ=255)

                                                           tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCgggg                                                                             >  2:1427816/1‑140 (MQ=255)

                                                           tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCgggg                                                                             >  1:690106/1‑140 (MQ=255)

                                                           tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCgggg                                                                             >  1:1178589/1‑140 (MQ=255)

                                                           tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCgggg                                                                             >  1:1798732/1‑140 (MQ=255)

                                                           tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCgggg                                                                             >  1:424597/1‑140 (MQ=255)

                                                           tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCgggg                                                                             >  2:2087226/1‑140 (MQ=255)

                                                           tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCgggg                                                                             >  2:658625/1‑140 (MQ=255)

                                                           tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCgggg                                                                             >  2:1803177/1‑140 (MQ=255)

                                                             aGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGAt                                                                           >  1:94250/1‑140 (MQ=255)

                                                              gCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATc                                                                          <  2:483720/140‑1 (MQ=255)

                                                                  gggCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTcc                                                                      >  1:1166538/1‑140 (MQ=255)

                                                                   ggCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCcg                                                                     <  1:3402/140‑1 (MQ=255)

                                                                   ggCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCcg                                                                     <  2:2204306/140‑1 (MQ=255)

                                                                   ggCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCcg                                                                     <  2:243843/140‑1 (MQ=255)

                                                                   ggCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCcg                                                                     <  2:1551547/140‑1 (MQ=255)

                                                                   ggCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCcg                                                                     <  1:833122/140‑1 (MQ=255)

                                                                   ggCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCcg                                                                     <  2:424597/140‑1 (MQ=255)

                                                                     cAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCcgcg                                                                   <  2:1785175/140‑1 (MQ=255)

                                                                     cAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCcgcg                                                                   <  1:1086098/140‑1 (MQ=255)

                                                                     cAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCcgcg                                                                   <  2:1161338/140‑1 (MQ=255)

                                                                     cAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCcgcg                                                                   <  1:1927970/140‑1 (MQ=255)

                                                                      aatccgcTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGa                                                                  <  1:2132041/134‑1 (MQ=255)

                                                                      aGTCGCCTGACCGCAGAGGTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGa                                                                  >  1:852073/1‑140 (MQ=255)

                                                                       gTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGAt                                                                 >  2:1540832/1‑140 (MQ=255)

                                                                        tCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATg                                                                >  1:781091/1‑140 (MQ=255)

                                                                             tGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTAt                                                           >  2:2185916/1‑140 (MQ=255)

                                                                                 cGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTgg                                                       <  2:1533188/140‑1 (MQ=255)

                                                                                 cGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTgg                                                       >  2:834082/1‑140 (MQ=255)

                                                                                 cGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTgg                                                       <  2:781091/140‑1 (MQ=255)

                                                                                   cAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTc                                                     >  1:2139747/1‑140 (MQ=255)

                                                                                         ttGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGaaaa                                               <  1:485611/140‑1 (MQ=255)

                                                                                         ttGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGaaaa                                               <  2:537513/140‑1 (MQ=255)

                                                                                             cATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACAcc                                           <  1:1742045/140‑1 (MQ=255)

                                                                                                  gcgcGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACg                                      >  2:2369841/1‑140 (MQ=255)

                                                                                                  gcgcGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACg                                      <  1:567813/140‑1 (MQ=255)

                                                                                                   cgcgATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGc                                     <  1:602955/140‑1 (MQ=255)

                                                                                                        tGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAACCACCTAACGCATAtt                                >  1:1133790/1‑140 (MQ=255)

                                                                                                        tGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATAtt                                <  2:2139747/140‑1 (MQ=255)

                                                                                                        tGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATAtt                                <  2:1178589/140‑1 (MQ=255)

                                                                                                        tGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATAtt                                <  1:1400714/140‑1 (MQ=255)

                                                                                                        tGGTTAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATAtt                                <  1:1492663/140‑1 (MQ=255)

                                                                                                           ttAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATAtt                                >  2:761851/1‑137 (MQ=255)

                                                                                                           ttAAAACCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATAtt                                <  1:761851/137‑1 (MQ=255)

                                                                                                             aaaaCCGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGa                           <  2:1018315/140‑1 (MQ=255)

                                                                                                                 ccGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTc                       <  1:1177508/140‑1 (MQ=255)

                                                                                                                  cGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCa                      <  1:2185916/140‑1 (MQ=255)

                                                                                                                  cGGCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCa                      <  2:1347054/140‑1 (MQ=255)

                                                                                                                    gCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAATGATTTCACt                    >  1:1106829/1‑140 (MQ=255)

                                                                                                                    gCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACt                    >  1:1764013/1‑140 (MQ=255)

                                                                                                                    gCGACAACCTGTTGTGTGTGCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACt                    >  1:2079155/1‑140 (MQ=255)

                                                                                                                                    tgtgCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTg    >  2:1085652/1‑140 (MQ=255)

                                                                                                                                    tgtgCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTg    >  1:1878975/1‑140 (MQ=255)

                                                                                                                                    tgtgCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTg    >  1:1141962/1‑140 (MQ=255)

                                                                                                                                      tgCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTGCCGACTTTGAc  <  2:852073/140‑1 (MQ=255)

                                                                                                                                      tgCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGAc  <  1:634470/140‑1 (MQ=255)

                                                                                                                                      tgCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGAc  <  1:415303/140‑1 (MQ=255)

                                                                                                                                      tgCTCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGAc  >  1:1107946/1‑140 (MQ=255)

TAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGAC  >  NC_000913/3223022‑3223295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`