Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,277,596	C→A	R433S (CGT→AGT)	yejF →	putative oligopeptide ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,277,596	0	C	A	100.0%	316.0 / NA	88	R433S (CGT→AGT)	yejF	putative oligopeptide ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (43/45);  total (43/45)

CTTACGGGTTCACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCATCAACTG  >  NC_000913/2277463‑2277730                                                                                                                                      |                                                                                                                                       cTTACGGGTTCACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTg                                                                                                                                  >  2:995204/1‑140 (MQ=255) cTTACGGGTTCACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTg                                                                                                                                  >  2:770516/1‑140 (MQ=255)    aCGGGTTCACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGa                                                                                                                               >  1:1011900/1‑140 (MQ=255)    aCGGGTTCACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGa                                                                                                                               >  1:1236434/1‑140 (MQ=255)        gTTCACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGc                                                                                                                           <  2:41450/140‑1 (MQ=255)         ttCACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGcc                                                                                                                          >  1:439015/1‑140 (MQ=255)          tCACCAGCCGACGCTTTCTGTCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCa                                                                                                                         >  2:359006/1‑140 (MQ=255)          tCACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCa                                                                                                                         <  1:141889/140‑1 (MQ=255)          tCACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCa                                                                                                                         <  1:67426/140‑1 (MQ=255)           cACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTa                                                                                                                                     >  1:958694/1‑127 (MQ=255)           cACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTa                                                                                                                                     <  2:958694/127‑1 (MQ=255)           cACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAg                                                                                                                        >  2:83737/1‑140 (MQ=255)           cACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAg                                                                                                                        >  1:182618/1‑140 (MQ=255)           cACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAg                                                                                                                        >  2:1227913/1‑140 (MQ=255)           cACCAGCCGACGCTTTCTGCCGCACAACGCG‑ACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAgg                                                                                                                       >  2:159339/1‑140 (MQ=255)                   gACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCTGGGCATTaa                                                                                                                <  2:439015/140‑1 (MQ=255)                   gACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTaa                                                                                                                >  1:964241/1‑140 (MQ=255)                    aCGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAAt                                                                                                               >  1:1398474/1‑140 (MQ=255)                    aCGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAAt                                                                                                               >  1:23374/1‑140 (MQ=255)                          tCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTaa                                                                                                         >  2:467675/1‑140 (MQ=255)                            tGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGc                                                                                                       <  2:1011900/140‑1 (MQ=255)                             gCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGcc                                                                                                      <  2:23374/140‑1 (MQ=255)                             gCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGcc                                                                                                      <  2:1027916/140‑1 (MQ=255)                              ccGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGccc                                                                                                     <  2:1220366/140‑1 (MQ=255)                                gCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTc                                                                                                   >  1:75686/1‑140 (MQ=255)                                gCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTc                                                                                                   <  1:711468/140‑1 (MQ=255)                                gCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTc                                                                                                   <  2:70424/140‑1 (MQ=255)                                 cacaACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCg                                                                                                  <  2:1236434/140‑1 (MQ=255)                                 cacaACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCg                                                                                                  <  2:781865/140‑1 (MQ=255)                                 cacaACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCg                                                                                                  <  2:97095/140‑1 (MQ=255)                                 cacaACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCg                                                                                                  <  2:1268942/140‑1 (MQ=255)                                 cacaACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCg                                                                                                  <  1:359006/140‑1 (MQ=255)                                  acaACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGc                                                                                                 >  1:574721/1‑140 (MQ=255)                                      cgcgAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGAt                                                                                             >  2:253378/1‑140 (MQ=255)                                          aacaacAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATa                                                                                         <  1:550299/140‑1 (MQ=255)                                                   tGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGaa                                                                                <  2:964241/140‑1 (MQ=255)                                                     aTAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAAcc                                                                              >  2:897018/1‑140 (MQ=255)                                                     aTAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAAcc                                                                              <  1:995204/140‑1 (MQ=255)                                                     aTAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAAcc                                                                              >  1:860526/1‑140 (MQ=255)                                                     aTAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAAcc                                                                              >  1:397794/1‑140 (MQ=255)                                                       aGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGa                                                                            >  1:714097/1‑140 (MQ=255)                                                       aGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGa                                                                            >  1:709383/1‑140 (MQ=255)                                                       aGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGa                                                                            <  1:83737/140‑1 (MQ=255)                                                          cGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGAcat                                                                         <  2:714097/140‑1 (MQ=255)                                                          cGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTCGATGAACCGAcat                                                                         >  1:115214/1‑140 (MQ=255)                                                             gATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGAcatcat                                                                      >  2:1219296/1‑140 (MQ=255)                                                               tGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACAtcatca                                                                    >  1:480458/1‑140 (MQ=255)                                                                    gAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGa                                                               <  2:853879/140‑1 (MQ=255)                                                                    gAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGa                                                               <  1:159339/140‑1 (MQ=255)                                                                      aGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACa                                                             <  2:860526/140‑1 (MQ=255)                                                                       gTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACaa                                                            <  1:705347/140‑1 (MQ=255)                                                                       gTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACaa                                                            <  1:253215/140‑1 (MQ=255)                                                                        tGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACaaa                                                           >  1:703256/1‑140 (MQ=255)                                                                        tGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACaaa                                                           >  1:229276/1‑140 (MQ=255)                                                                         gggATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACaaaa                                                          >  1:1319335/1‑140 (MQ=255)                                                                            aTTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACgg                                                       >  1:235646/1‑140 (MQ=255)                                                                              tAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTa                                                     <  2:1398474/140‑1 (MQ=255)                                                                              tAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTa                                                     <  2:397794/140‑1 (MQ=255)                                                                              tAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTa                                                     <  1:1437804/140‑1 (MQ=255)                                                                              tAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTa                                                     <  2:229276/140‑1 (MQ=255)                                                                              tAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGAAATCATCACTCGACAAAACGGTa                                                     <  2:700389/140‑1 (MQ=255)                                                                               aGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTAc                                                    <  1:770516/140‑1 (MQ=255)                                                                                  tCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAgg                                                 >  2:1285638/1‑140 (MQ=255)                                                                                  tCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAgg                                                 >  2:942175/1‑140 (MQ=255)                                                                                     tGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGgcgc                                              >  1:441417/1‑140 (MQ=255)                                                                                     tGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGgcgc                                              >  1:407690/1‑140 (MQ=255)                                                                                     tGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGgcgc                                              <  2:1224537/140‑1 (MQ=255)                                                                                     tGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGgcgc                                              >  1:1332768/1‑140 (MQ=255)                                                                                      gAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACaaaa                                                          <  1:72637/127‑1 (MQ=255)                                                                                      gAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACaaaa                                                          >  2:72637/1‑127 (MQ=255)                                                                                           acGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAatat                                        <  1:569820/140‑1 (MQ=255)                                                                                              ccACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGa                                     <  2:407690/140‑1 (MQ=255)                                                                                              ccACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTCGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGa                                     <  2:115214/140‑1 (MQ=255)                                                                                                aCCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGCCATGCGCACTCGACAAAACGGTACAGGCGCAAATATTGACg                                   <  2:448535/140‑1 (MQ=255)                                                                                                aCCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACg                                   <  2:480458/140‑1 (MQ=255)                                                                                                aCCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACg                                   <  1:213092/140‑1 (MQ=255)                                                                                                aCCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACg                                   <  2:441417/140‑1 (MQ=255)                                                                                                aCCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACg                                   <  2:235646/140‑1 (MQ=255)                                                                                                  cGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCt                                 >  2:1433367/1‑140 (MQ=255)                                                                                                  cGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCt                                 >  2:992671/1‑140 (MQ=255)                                                                                                            cggAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGAc                                    <  2:726543/127‑1 (MQ=255)                                                                                                            cggAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGAc                                    >  1:726543/1‑127 (MQ=255)                                                                                                            cggAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCa                       >  1:610462/1‑140 (MQ=255)                                                                                                            cggAGTTCTCTGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCa                       >  1:222930/1‑140 (MQ=255)                                                                                                                    tctGGTGGTCAGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGcaaca               <  1:897018/140‑1 (MQ=255)                                                                                                                              aGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCATCaa     >  1:1320721/1‑140 (MQ=255)                                                                                                                              aGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCATCaa     >  2:886547/1‑140 (MQ=255)                                                                                                                              aGCGACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCATCaa     <  1:1202734/140‑1 (MQ=255)                                                                                                                                 gACAAAGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCATCAACTg  <  1:992671/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       CTTACGGGTTCACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCATCAACTG  >  NC_000913/2277463‑2277730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑