Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,424,614	G→T	A207E (GCG→GAG)	hisM ←	lysine/arginine/ornithine ABC transporter/histidine ABC transporter, membrane subunit HisM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,424,614	0	G	T	100.0%	321.9 / NA	90	A207E (GCG→GAG)	hisM	lysine/arginine/ornithine ABC transporter/histidine ABC transporter, membrane subunit HisM
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (55/35);  total (55/35)

TAACGTTTAATTTATTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGC  >  NC_000913/2424485‑2424750                                                                                                                                  |                                                                                                                                         tAACGTTTAATTTATTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGcc                                                                                                                                >  2:1357446/1‑140 (MQ=255)  aaCGTTTAATTTATTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCg                                                                                                                               <  2:272656/140‑1 (MQ=255)  aaCGTTTAATTTATTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCg                                                                                                                               <  2:910060/140‑1 (MQ=255)    cGTTTAATTTATTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTACAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGaa                                                                                                                             <  1:1304148/140‑1 (MQ=255)      tttAATTTATTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAgg                                                                                                                           <  1:859398/140‑1 (MQ=255)      tttAATTTATTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAgg                                                                                                                           <  2:988644/140‑1 (MQ=255)      tttAATTTATTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAgg                                                                                                                           <  1:374672/140‑1 (MQ=255)      tttAATTTATTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAgg                                                                                                                           <  1:2642/140‑1 (MQ=255)           tttATTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTa                                                                                                                      >  2:886124/1‑140 (MQ=255)            ttATTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTaa                                                                                                                     <  1:886124/140‑1 (MQ=255)            ttATTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTaa                                                                                                                     <  1:560033/140‑1 (MQ=255)              aTTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTaaaa                                                                                                                   >  2:1420518/1‑140 (MQ=255)               ttCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAg                                                                                                                  >  1:389435/1‑140 (MQ=255)                          cGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGTCATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTg                                                                                                       >  1:45449/1‑140 (MQ=255)                            tATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTggc                                                                                                     <  2:389435/140‑1 (MQ=255)                                ctcAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGgcggcg                                                                                                 >  2:778721/1‑140 (MQ=255)                                  cAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGtt                                                                                               >  1:674376/1‑140 (MQ=255)                                  cAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGtt                                                                                               >  2:637260/1‑140 (MQ=255)                                   aGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTa                                                                                              >  2:112728/1‑140 (MQ=255)                                   aGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTa                                                                                              >  1:364111/1‑140 (MQ=255)                                   aGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTa                                                                                              >  2:675227/1‑140 (MQ=255)                                    gTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTaa                                                                                             >  2:913960/1‑140 (MQ=255)                                    gTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTaa                                                                                             >  1:274698/1‑140 (MQ=255)                                    gTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTaa                                                                                             >  1:778517/1‑140 (MQ=255)                                     tGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTggc                                                                                                     <  1:967478/131‑1 (MQ=255)                                     tGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTggc                                                                                                     >  2:967478/1‑131 (MQ=255)                                     tGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAat                                                                                            >  2:125646/1‑140 (MQ=255)                                           gaagaaGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATcgcg                                                                                      >  2:1126954/1‑140 (MQ=255)                                             agaagGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCggg                                                                                    <  1:913960/140‑1 (MQ=255)                                                   tttCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTAttt                                                                              >  1:1043947/1‑140 (MQ=255)                                                   tttCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTAttt                                                                              >  1:245443/1‑140 (MQ=255)                                                      cacaTGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTca                                                                           >  1:795843/1‑140 (MQ=255)                                                      cacaTGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTca                                                                           >  2:1138235/1‑140 (MQ=255)                                                        caTGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTcagc                                                                         <  2:795843/140‑1 (MQ=255)                                                        caTGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTcagc                                                                         <  1:112728/140‑1 (MQ=255)                                                        caTGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTcagc                                                                         <  2:674376/140‑1 (MQ=255)                                                            ctgcAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGAt                                                                     >  2:1107938/1‑140 (MQ=255)                                                             tgcAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATc                                                                    >  2:738062/1‑140 (MQ=255)                                                               cAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCg                                                                  >  1:991671/1‑140 (MQ=255)                                                               cAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCg                                                                  >  1:1146802/1‑140 (MQ=255)                                                                     gCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCg                                                            >  1:1002164/1‑140 (MQ=255)                                                                           ttCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAg                                                      >  2:1371433/1‑140 (MQ=255)                                                                             ccGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTa                                                    <  1:1178619/140‑1 (MQ=255)                                                                                ctctGCGAAAGAGGCTGATGAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGCTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCACCAGATCCGGCACCGTGGCAGTAAAt                                                 >  2:968138/1‑140 (MQ=255)                                                                                ctctGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAAt                                                 >  1:646813/1‑140 (MQ=255)                                                                                ctctGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAAt                                                 >  2:254929/1‑140 (MQ=255)                                                                                ctctGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAAt                                                 <  1:1420518/140‑1 (MQ=255)                                                                                  ctGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGc                                               <  2:646813/140‑1 (MQ=255)                                                                                  ctGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGc                                               >  1:728434/1‑140 (MQ=255)                                                                                   tGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGcc                                              >  1:452821/1‑140 (MQ=255)                                                                                    gCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCa                                             <  1:1107938/140‑1 (MQ=255)                                                                                    gCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCa                                             <  1:761262/140‑1 (MQ=255)                                                                                         agagGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCa                                        <  1:1357446/140‑1 (MQ=255)                                                                                             gCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTag                                    >  1:324919/1‑140 (MQ=255)                                                                                             gCTGATCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTag                                    >  2:1054150/1‑140 (MQ=255)                                                                                                 aTCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTg                                <  1:1395654/140‑1 (MQ=255)                                                                                                 aTCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTg                                <  1:1138235/140‑1 (MQ=255)                                                                                                 aTCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTg                                <  1:778721/140‑1 (MQ=255)                                                                                                 aTCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTg                                <  1:531098/140‑1 (MQ=255)                                                                                                  tCAGGACATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTgc                               >  1:791399/1‑140 (MQ=255)                                                                                                       aCATAAGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCAt                          <  2:991671/140‑1 (MQ=255)                                                                                                           aaGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAgg                      >  2:168559/1‑140 (MQ=255)                                                                                                           aaGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAgg                      >  1:1332292/1‑140 (MQ=255)                                                                                                           aaGAGATGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAgg                      >  2:88644/1‑140 (MQ=255)                                                                                                             gagaTGATTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGTAGCATCAGGAt                    <  1:1414984/140‑1 (MQ=255)                                                                                                                atgatTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCAt                          <  1:615821/131‑1 (MQ=255)                                                                                                                atgatTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCAt                          >  2:615821/1‑131 (MQ=255)                                                                                                                atgatTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCAc                 >  1:225472/1‑140 (MQ=255)                                                                                                                atgatTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCAc                 >  2:657497/1‑140 (MQ=255)                                                                                                                atgatTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCAc                 >  1:1377473/1‑140 (MQ=255)                                                                                                                atgatTAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCAc                 >  2:177513/1‑140 (MQ=255)                                                                                                                    ttAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCg             <  1:88644/140‑1 (MQ=255)                                                                                                                    ttAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCg             <  2:274698/140‑1 (MQ=255)                                                                                                                    ttAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCg             <  1:1371433/140‑1 (MQ=255)                                                                                                                    ttAAATAGAGCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCg             <  1:177513/140‑1 (MQ=255)                                                                                                                     tAAATAGAGCGCCTCGGCAAAGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGGAACGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGt            <  1:637260/140‑1 (MQ=255)                                                                                                                            agCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTa     >  2:1000217/1‑140 (MQ=255)                                                                                                                            agCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTa     >  1:336304/1‑140 (MQ=255)                                                                                                                            agCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTa     >  1:129241/1‑140 (MQ=255)                                                                                                                            agCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTa     >  2:66158/1‑140 (MQ=255)                                                                                                                            agCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTa     >  2:596442/1‑140 (MQ=255)                                                                                                                             gCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTAt    >  2:666656/1‑140 (MQ=255)                                                                                                                             gCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTAt    >  2:815022/1‑140 (MQ=255)                                                                                                                             gCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTAt    <  2:336304/140‑1 (MQ=255)                                                                                                                             gCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTAt    >  1:897175/1‑140 (MQ=255)                                                                                                                             gCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTAt    >  1:1316988/1‑140 (MQ=255)                                                                                                                             gCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTAt    >  2:1249242/1‑140 (MQ=255)                                                                                                                             gCACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTAt    >  1:1093417/1‑140 (MQ=255)                                                                                                                              cACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGc         <  1:371273/134‑1 (MQ=255)                                                                                                                              cACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGc         >  2:371273/1‑134 (MQ=255)                                                                                                                              cACCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATg   >  1:91413/1‑140 (MQ=255)                                                                                                                               aCCTCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGc  <  1:596442/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                         TAACGTTTAATTTATTCTCGGACATCGTATTCTCAGTGCGTTGAAGAAGGTTTCACATGCTGCAACCAGCGTTTTTCCGCTCTGCGAAAGAGGCTGATCAGGACATAAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGC  >  NC_000913/2424485‑2424750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑