Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,511,677	C→A	D344Y (GAT→TAT)	mntH ←	Mn(2(+))/Fe(2(+)): H(+) symporter MntH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,511,677	0	C	A	100.0%	180.3 / NA	51	D344Y (GAT→TAT)	mntH	Mn(2(+))/Fe(2(+)): H(+) symporter MntH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (22/29);  total (22/29)

TCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGA  >  NC_000913/2511542‑2511806                                                                                                                                        |                                                                                                                                  tctgtTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAAt                                                                                                                               >  1:1016814/1‑140 (MQ=255) tctgtTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAAt                                                                                                                               >  2:9316/1‑140 (MQ=255) tctgtTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAAt                                                                                                                               >  1:24073/1‑140 (MQ=255) tctgtTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAAt                                                                                                                               >  2:363457/1‑140 (MQ=255)    gtTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATccc                                                                                                                            <  1:1332603/140‑1 (MQ=255)          cgcgTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGGACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGa                                                                                                                      <  2:1167896/140‑1 (MQ=255)           gcgTTTGCTGTTCACCAGATCGCCCATCAACTTGGTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGaa                                                                                                                     <  2:670522/140‑1 (MQ=255)           gcgTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGaa                                                                                                                     <  1:717086/140‑1 (MQ=255)            cgTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAAt                                                                                                                    <  2:1027124/140‑1 (MQ=255)              tttGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAATGa                                                                                                                  <  1:540441/140‑1 (MQ=255)               ttGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGaa                                                                                                                     <  2:911818/136‑1 (MQ=255)               ttGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGaa                                                                                                                     >  1:911818/1‑136 (MQ=255)               ttGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAATGAc                                                                                                                 >  2:1230186/1‑140 (MQ=255)                tGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAATGACa                                                                                                                >  1:1103579/1‑140 (MQ=255)                    gTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAATGACAATaa                                                                                                            <  2:1036223/140‑1 (MQ=255)                       cACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAAt                                                                                                                    <  1:448732/129‑1 (MQ=255)                       cACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAAt                                                                                                                    >  2:448732/1‑129 (MQ=255)                        aCCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAAACCGTGTCGGATATAATCCCATCAGAATGACAATAAATGa                                                                                                        <  2:698026/140‑1 (MQ=255)                         ccAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAATGACAATAAATGAc                                                                                                       >  1:1072453/1‑140 (MQ=255)                           aGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAATGACAATAAATGACgg                                                                                                     <  1:9316/140‑1 (MQ=255)                            gATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAATGACAATAAATGACGGc                                                                                                    <  1:66192/140‑1 (MQ=255)                            gATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAATGACAATAAATGACGGc                                                                                                    <  2:233483/140‑1 (MQ=255)                                 cccATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAATGACAATAAATGACGGCAACAt                                                                                               <  2:462685/140‑1 (MQ=255)                                    aTCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAATGACAATAAATGACGGCAACATGGt                                                                                            <  2:24073/140‑1 (MQ=255)                                    aTCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAATGACAATAAATGACGGCAACATGGt                                                                                            <  2:1016814/140‑1 (MQ=255)                                     tCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAATGACAATAAATGACGGCAACATGGTg                                                                                           <  2:349360/140‑1 (MQ=255)                                       aaCTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGAc                                                                                         >  1:409284/1‑140 (MQ=255)                                          ttGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGt                                                                                      <  1:504428/140‑1 (MQ=255)                                              tGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTacga                                                                                  >  2:573165/1‑140 (MQ=255)                                              tGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTacga                                                                                  <  1:1230186/140‑1 (MQ=255)                                               gTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTacgac                                                                                 <  1:44633/140‑1 (MQ=255)                                                    tGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCAcc                                                                            >  1:1019648/1‑140 (MQ=255)                                                        gAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCcaca                                                                        <  2:596398/140‑1 (MQ=255)                                                         aaaaTCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAg                                                                       <  1:573165/140‑1 (MQ=255)                                                         aaaaTCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAg                                                                       <  1:363457/140‑1 (MQ=255)                                                                gcagTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGata                                                                >  2:905225/1‑140 (MQ=255)                                                                gcagTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGata                                                                >  1:664512/1‑140 (MQ=255)                                                                           aGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCg                                                                     >  1:120510/1‑124 (MQ=255)                                                                           aGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCg                                                                     <  2:120510/124‑1 (MQ=255)                                                                              gcCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGaa                                                  >  1:899663/1‑140 (MQ=255)                                                                               ccAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAg                                                 <  2:664512/140‑1 (MQ=255)                                                                               ccAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAg                                                 <  2:855955/140‑1 (MQ=255)                                                                                cAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGc                                                >  2:861787/1‑140 (MQ=255)                                                                                cAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGc                                                <  1:861787/140‑1 (MQ=255)                                                                                 agagCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGcc                                               >  1:755196/1‑140 (MQ=255)                                                                                      gATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCa                                          <  1:279096/140‑1 (MQ=255)                                                                                                        tACCTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACCCCCCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCCCCCCCTGCCCCGCCa                        <  2:1072453/140‑1 (MQ=255)                                                                                                          ccTGACTCATAACCAGAATCCGTGTCGGATATAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAgt                      >  1:1431651/1‑140 (MQ=255)                                                                                                                  aTACCCAGAATCCAGGTCGGATATAATCCCATCAGAATGACAAAAACTGACGGGAACATGGTGACTAAACGAGGCACCCACAGCGGGATATGAAAGCGAATGAAGCACTGCATCACCACCCACCCCGACAAAGTcaccac              >  2:103299/1‑140 (MQ=255)                                                                                                                    aaCCAGAACCCGTGTGGGATATAACCCCATCAGAATGACAAAAAATGACGGCAACATGGTGACTGTACGACGCCCCCACGGCGGGATATGAAAGCGAATGAAGCCCTGCATCCCCCCCTGCCCCGCCAGTGTCCccacca            <  2:755196/140‑1 (MQ=255)                                                                                                                        aGAATCCGTGTCGGATATAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGt        >  1:1161889/1‑140 (MQ=255)                                                                                                                            tCCGTGTCGGATATAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGaa    >  1:941911/1‑140 (MQ=255)                                                                                                                              cGTGTCGGATATAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGa  >  2:1048173/1‑140 (MQ=255)                                                                                                                              cGTGTCGGATATAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCAAGTGTCCCCACCACCGTTGAAGa  <  1:1373834/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                  TCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGA  >  NC_000913/2511542‑2511806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑